Restriction map for
pF1KB0449
(Circular) (Six-base) MAPSORT of: pF1KB0449.seq Check: 2959 from: 1 to: 4535
pF1KB0449 4535 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AatII G_ACGT'C
Cuts at: 386 386
Size: 4535
AccI GT'mk_AC
Cuts at: 1573 1573
Size: 4535
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 718 805 925 964 1459 2360 2792 3539
Size: 9 87 120 39 495 901 432 747
Cuts at: 3539 3807 709
Size: 268 1437
Fragments arranged by size:
1437 901 747 495 432 268 120 87
39 9
AflIII A'CryG_T
Cuts at: 1887 2991 3968 1887
Size: 1104 977 2454
Fragments arranged by size:
2454 1104 977
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AleI CACnn'nnGTG
Cuts at: 757 757
Size: 4535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 795 918 1360 1840 3407 795
Size: 123 442 480 1567 1923
Fragments arranged by size:
1923 1567 480 442 123
ApaLI G'TGCA_C
Cuts at: 1402 3305 1402
Size: 1903 2632
ApoI r'AATT_y
Cuts at: 193 430 1539 3888 4144 193
Size: 237 1109 2349 256 584
Fragments arranged by size:
2349 1109 584 256 237
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1131 1177 1555 1131
Size: 46 378 4111
Fragments arranged by size:
4111 378 46
BamHI G'GATC_C
Cuts at: 1048 1560 1048
Size: 512 4023
BanI G'GyrC_C
Cuts at: 565 1551 1965 2213 2248 565
Size: 986 414 248 35 2852
Fragments arranged by size:
2852 986 414 248 35
BanII G_rGCy'C
Cuts at: 460 1138 1549 2579 460
Size: 678 411 1030 2416
Fragments arranged by size:
2416 1030 678 411
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 573 1421 573
Size: 848 3687
BciVI GTATCCnnnnn_n'
Cuts at: 1284 2433 3194 4535 1284
Size: 1149 761 1341 1284
Fragments arranged by size:
1341 1284 1149 761
BclI T'GATC_A
Cuts at: 110 2055 110
Size: 1945 2590
BglII A'GATC_T
Cuts at: 462 2050 4087 462
Size: 1588 2037 910
Fragments arranged by size:
2037 1588 910
BlpI GC'TnA_GC
Cuts at: 478 1647 478
Size: 1169 3366
Bme1580I G_kGCm'C
Cuts at: 161 568 986 1406 2160 2253 3309 161
Size: 407 418 420 754 93 1056 1387
Fragments arranged by size:
1387 1056 754 420 418 407 93
BmgBI CAC'GTC
Cuts at: 1199 1199
Size: 4535
BmrI ACTGGGnnnn_n'
Cuts at: 565 1747 1828 2163 4307 565
Size: 1182 81 335 2144 793
Fragments arranged by size:
2144 1182 793 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 416 2902 416
Size: 2486 2049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1438 658
Size: 780 3755
Bpu10I CC'TnA_GC
Cuts at: 1312 1312
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1711 2590 3082 3380 1711
Size: 879 492 298 2866
Fragments arranged by size:
2866 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 257 1391 4239 163
Size: 94 1134 2848 459
Fragments arranged by size:
2848 1134 459 94
BsaAI yAC'GTr
Cuts at: 540 1888 540
Size: 1348 3187
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 383 1966 2214 2916 4412 383
Size: 1583 248 702 1496 506
Fragments arranged by size:
1583 1496 702 506 248
BsaWI w'CCGG_w
Cuts at: 1382 1724 2245 2923 3197 3344 3755 1382
Size: 342 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 342 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1385 1408 1415 1438 1385
Size: 23 7 23 4482
Fragments arranged by size:
4482 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 661 1165 325
Size: 336 504 3695
Fragments arranged by size:
3695 504 336
BseYI C'CCAG_C
Cuts at: 1044 1960 2718 3295 3791 1044
Size: 916 758 577 496 1788
Fragments arranged by size:
1788 916 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 358 645 877 1141 300
Size: 58 287 232 264 3694
Fragments arranged by size:
3694 287 264 232 58
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3331 104
Size: 1973 46 1208 1308
Fragments arranged by size:
1973 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 644 1406 1549 2327 2517 3309 644
Size: 762 143 778 190 792 1870
Fragments arranged by size:
1870 792 778 762 190 143
BsmBI CGTCTCn'nnnn_
Cuts at: 1189 1189
Size: 4535
Bsp1286I G_dGCh'C
Cuts at: 161 460 568 644 986 1138 1406 1549 2160
Size: 299 108 76 342 152 268 143 611
Cuts at: 2160 2253 2327 2517 2579 3309 161
Size: 93 74 190 62 730 1387
Fragments arranged by size:
1387 730 611 342 299 268 190 152
143 108 93 76 74 62
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 113 815 3711 113
Size: 702 2896 937
Fragments arranged by size:
2896 937 702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1585 1799 2101 2482 926
Size: 659 214 302 381 2979
Fragments arranged by size:
2979 659 381 302 214
BsrBI CCG'CTC
Cuts at: 50 477 1266 2827 2881 4365 4530 50
Size: 427 789 1561 54 1484 165 55
Fragments arranged by size:
1561 1484 789 427 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2447 2447
Size: 4535
BsrFI r'CCGG_y
Cuts at: 224 2533 2714 2923 224
Size: 2309 181 209 1836
Fragments arranged by size:
2309 1836 209 181
BssSI C'ACGA_G
Cuts at: 643 2806 3164 643
Size: 2163 358 2014
Fragments arranged by size:
2163 2014 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1912 1360
Size: 552 3983
BstBI TT'CG_AA
Cuts at: 2896 2896
Size: 4535
BstEII G'GTnAC_C
Cuts at: 1011 1011
Size: 4535
BstXI CCAn_nnnn'nTGG
Cuts at: 1008 1008
Size: 4535
BstYI r'GATC_y
Cuts at: 1 462 629 887 1048 1445 1560 2027 2050
Size: 461 167 258 161 397 115 467 23
Cuts at: 2050 2385 2631 3643 3729 3741 4087 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 461 449 397 346 335 258
246 167 161 115 86 23 12
BtgI C'CryG_G
Cuts at: 374 407 374
Size: 33 4502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 662 1418 1778 662
Size: 756 360 3419
Fragments arranged by size:
3419 756 360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1053 1088 1053
Size: 35 4500
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3099 2241
Size: 858 3677
EaeI y'GGCC_r
Cuts at: 950 965 1109 2120 2294 2685 2712 4057 950
Size: 15 144 1011 174 391 27 1345 1428
Fragments arranged by size:
1428 1345 1011 391 174 144 27 15
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 1132 1187 2558 2768 1132
Size: 55 1371 210 2899
Fragments arranged by size:
2899 1371 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 243 406 2743 3053 3199 4345 243
Size: 163 2337 310 146 1146 433
Fragments arranged by size:
2337 1146 433 310 163 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 709 718 805 925 964 1438 1459 2360
Size: 51 9 87 120 39 474 21 901
Cuts at: 2360 2792 3539 3807 658
Size: 432 747 268 1386
Fragments arranged by size:
1386 901 747 474 432 268 120 87
51 39 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 424 424
Size: 4535
EcoO109I rG'GnC_Cy
Cuts at: 861 900 1028 1121 1181 1461 1674 861
Size: 39 128 93 60 280 213 3722
Fragments arranged by size:
3722 280 213 128 93 60 39
EcoRI G'AATT_C
Cuts at: 193 430 1539 193
Size: 237 1109 3189
Fragments arranged by size:
3189 1109 237
EcoRV GAT'ATC
Cuts at: 414 414
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4535
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 141 366 658 1969 2217 3239 4218 141
Size: 225 292 1311 248 1022 979 458
Fragments arranged by size:
1311 1022 979 458 292 248 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 447 479 1408 1440 2377 2407 2409 2439 447
Size: 32 929 32 937 30 2 30 2543
Fragments arranged by size:
2543 937 929 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1574 1574
Size: 4535
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1268 3206 3390 1268
Size: 1938 184 2413
Fragments arranged by size:
2413 1938 184
MscI TGG'CCA
Cuts at: 967 1111 2296 967
Size: 144 1185 3206
Fragments arranged by size:
3206 1185 144
MslI CAynn'nnrTG
Cuts at: 112 757 820 1075 2524 2651 112
Size: 645 63 255 1449 127 1996
Fragments arranged by size:
1996 1449 645 255 127 63
MspA1I CmG'CkG
Cuts at: 305 348 852 1646 1960 2320 3333 3578 305
Size: 43 504 794 314 360 1013 245 1262
Fragments arranged by size:
1262 1013 794 504 360 314 245 43
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 374 407 374
Size: 33 4502
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NspI r_CATG'y
Cuts at: 489 825 1350 1588 2088 2995 489
Size: 336 525 238 500 907 2029
Fragments arranged by size:
2029 907 525 500 336 238
PciI A'CATG_T
Cuts at: 2991 2991
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PpuMI rG'GwC_Cy
Cuts at: 900 1181 1461 900
Size: 281 280 3974
Fragments arranged by size:
3974 281 280
PstI C_TGCA'G
Cuts at: 1582 2267 1582
Size: 685 3850
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 852 1960 2320 852
Size: 1108 360 3067
Fragments arranged by size:
3067 1108 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SanDI GG'GwC_CC
Cuts at: 1181 1181
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 1060 4052 1060
Size: 2992 1543
SfcI C'TryA_G
Cuts at: 33 436 445 1236 1578 2263 3256 3447 33
Size: 403 9 791 342 685 993 191 1121
Fragments arranged by size:
1121 993 791 685 403 342 191 9
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1133 1557 1133
Size: 424 4111
SmlI C'TyrA_G
Cuts at: 1690 2605 3097 3359 1690
Size: 915 492 262 2866
Fragments arranged by size:
2866 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 825 1588 2088 825
Size: 763 500 3272
Fragments arranged by size:
3272 763 500
SspI AAT'ATT
Cuts at: 711 711
Size: 4535
StyI C'CwwG_G
Cuts at: 185 374 407 1669 2023 185
Size: 189 33 1262 354 2697
Fragments arranged by size:
2697 1262 354 189 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 734 2118 2251 2710 2919 238
Size: 496 1384 133 459 209 1854
Fragments arranged by size:
1854 1384 496 459 209 133
TatI w'GTAC_w
Cuts at: 1058 4050 1058
Size: 2992 1543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1385 1417 4052 4084 1385
Size: 32 2635 32 1836
Fragments arranged by size:
2635 1836 32 32
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 4535
XmaI C'CCGG_G
Cuts at: 1131 1555 1131
Size: 424 4111
ZraI GAC'GTC
Cuts at: 384 384
Size: 4535
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI