Restriction map for
pF1KB0452
(Circular) (Six-base) MAPSORT of: pF1KB0452.seq Check: 7289 from: 1 to: 5870
pF1KB0452 5870 bp
With 182 enzymes: SgfI *
November 15, 2011 13:19 ..
AccI GT'mk_AC
Cuts at: 137 1129 2908 137
Size: 992 1779 3099
Fragments arranged by size:
3099 1779 992
Acc65I G'GTAC_C
Cuts at: 1613 2886 1613
Size: 1273 4597
AclI AA'CG_TT
Cuts at: 5710 5710
Size: 5870
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1031 1889 2020 2529 3695 4127 4874 5142
Size: 364 858 131 509 1166 432 747 268
Cuts at: 5142 667
Size: 1395
Fragments arranged by size:
1395 1166 858 747 509 432 364 268
131
AfeI AGC'GCT
Cuts at: 1092 1092
Size: 5870
AflII C'TTAA_G
Cuts at: 621 621
Size: 5870
AflIII A'CryG_T
Cuts at: 1831 2247 2292 3222 4326 5303 1831
Size: 416 45 930 1104 977 2398
Fragments arranged by size:
2398 1104 977 930 416 45
AgeI A'CCGG_T
Cuts at: 4258 4258
Size: 5870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 550 2729 550
Size: 2179 3691
AlwNI CAG_nnn'CTG
Cuts at: 183 505 546 1295 1572 2082 3175 4742 183
Size: 322 41 749 277 510 1093 1567 1311
Fragments arranged by size:
1567 1311 1093 749 510 322 277 41
ApaI G_GGCC'C
Cuts at: 223 607 1681 223
Size: 384 1074 4412
Fragments arranged by size:
4412 1074 384
ApaLI G'TGCA_C
Cuts at: 4640 4640
Size: 5870
ApoI r'AATT_y
Cuts at: 1921 2515 2581 2874 5223 5479 1921
Size: 594 66 293 2349 256 2312
Fragments arranged by size:
2349 2312 594 293 256 66
AseI AT'TA_AT
Cuts at: 20 20
Size: 5870
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5870
AvaI C'yCGr_G
Cuts at: 216 481 744 2890 216
Size: 265 263 2146 3196
Fragments arranged by size:
3196 2146 265 263
AvrII C'CTAG_G
Cuts at: 1074 1074
Size: 5870
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1867 1900 1867
Size: 33 5837
BamHI G'GATC_C
Cuts at: 2895 2895
Size: 5870
BanI G'GyrC_C
Cuts at: 227 259 1613 2471 2886 3300 3548 3583 227
Size: 32 1354 858 415 414 248 35 2514
Fragments arranged by size:
2514 1354 858 415 414 248 35 32
BanII G_rGCy'C
Cuts at: 223 607 753 1108 1681 1827 1992 2339 2884
Size: 384 146 355 573 146 165 347 545
Cuts at: 2884 3914 223
Size: 1030 2179
Fragments arranged by size:
2179 1030 573 545 384 355 347 165
146 146
BbeI G_GCGC'C
Cuts at: 263 3304 3552 263
Size: 3041 248 2581
Fragments arranged by size:
3041 2581 248
BbsI GAAGACnn'nnnn_
Cuts at: 1783 1842 1945 1783
Size: 59 103 5708
Fragments arranged by size:
5708 103 59
BbvCI CC'TCA_GC
Cuts at: 1383 1383
Size: 5870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1153 1187 2179 2213 2706 2740 1153
Size: 34 992 34 493 34 4283
Fragments arranged by size:
4283 992 493 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 717 3768 4529 5870 717
Size: 3051 761 1341 717
Fragments arranged by size:
3051 1341 761 717
BclI T'GATC_A
Cuts at: 3390 3390
Size: 5870
BglI GCCn_nnn'nGGC
Cuts at: 416 517 748 961 1300 1346 416
Size: 101 231 213 339 46 4940
Fragments arranged by size:
4940 339 231 213 101 46
BglII A'GATC_T
Cuts at: 3385 5422 3385
Size: 2037 3833
BlpI GC'TnA_GC
Cuts at: 2982 2982
Size: 5870
Bme1580I G_kGCm'C
Cuts at: 223 607 787 957 1643 1681 2819 3495 3588
Size: 384 180 170 686 38 1138 676 93
Cuts at: 3588 4644 223
Size: 1056 1449
Fragments arranged by size:
1449 1138 1056 686 676 384 180 170
93 38
BmrI ACTGGGnnnn_n'
Cuts at: 1643 2236 3082 3163 3498 5642 1643
Size: 593 846 81 335 2144 1871
Fragments arranged by size:
2144 1871 846 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 588 2820 4237 588
Size: 2232 1417 2221
Fragments arranged by size:
2232 2221 1417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 527 1288 2367 490
Size: 37 761 1079 3993
Fragments arranged by size:
3993 1079 761 37
Bpu10I CC'TnA_GC
Cuts at: 655 1383 655
Size: 728 5142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2649 3046 3925 4417 4715 2465
Size: 184 397 879 492 298 3620
Fragments arranged by size:
3620 879 492 397 298 184
BsaI GGTCTCn'nnnn_
Cuts at: 5574 5574
Size: 5870
BsaAI yAC'GTr
Cuts at: 2250 2295 3223 2250
Size: 45 928 4897
Fragments arranged by size:
4897 928 45
BsaBI GATnn'nnATC
Cuts at: 6 3384 5421 6
Size: 3378 2037 455
Fragments arranged by size:
3378 2037 455
BsaHI Gr'CG_yC
Cuts at: 260 3301 3549 4251 5747 260
Size: 3041 248 702 1496 383
Fragments arranged by size:
3041 1496 702 383 248
BsaWI w'CCGG_w
Cuts at: 558 1150 1699 2781 3059 3580 4258 4532 4679
Size: 592 549 1082 278 521 678 274 147
Cuts at: 4679 5090 558
Size: 411 1338
Fragments arranged by size:
1338 1082 678 592 549 521 411 278
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 500 530 500
Size: 30 5840
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 182 2080 182
Size: 1898 3972
BseYI C'CCAG_C
Cuts at: 126 600 3295 4053 4630 5126 126
Size: 474 2695 758 577 496 870
Fragments arranged by size:
2695 870 758 577 496 474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1376 2725 136
Size: 1240 1349 3281
Fragments arranged by size:
3281 1349 1240
BsiEI CG_ry'CG
Cuts at: 104 148 2625 3412 3458 4666 104
Size: 44 2477 787 46 1208 1308
Fragments arranged by size:
2477 1308 1208 787 46 44
BsiHKAI G_wGCw'C
Cuts at: 1009 1108 1158 1827 2884 3662 3852 4644 1009
Size: 99 50 669 1057 778 190 792 2235
Fragments arranged by size:
2235 1057 792 778 669 190 99 50
BsmI GAATG_Cn'
Cuts at: 973 973
Size: 5870
Bsp1286I G_dGCh'C
Cuts at: 223 607 753 787 957 1009 1108 1158 1643
Size: 384 146 34 170 52 99 50 485
Cuts at: 1643 1681 1827 1992 2339 2819 2884 3495 3588
Size: 38 146 165 347 480 65 611 93
Cuts at: 3588 3662 3852 3914 4644 223
Size: 74 190 62 730 1449
Fragments arranged by size:
1449 730 611 485 480 384 347 190
170 165 146 146 99 93 74 65
62 52 50 38 34
BspEI T'CCGG_A
Cuts at: 558 1150 1699 2781 5090 558
Size: 592 549 1082 2309 1338
Fragments arranged by size:
2309 1338 1082 592 549
BspHI T'CATG_A
Cuts at: 5046 5046
Size: 5870
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1789 2753 2920 3134 3436 3817 1789
Size: 964 167 214 302 381 3842
Fragments arranged by size:
3842 964 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 4162 4216 5700 5865 50
Size: 4112 54 1484 165 55
Fragments arranged by size:
4112 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2630 3782 2630
Size: 1152 4718
BsrFI r'CCGG_y
Cuts at: 3868 4049 4258 3868
Size: 181 209 5480
Fragments arranged by size:
5480 209 181
BsrGI T'GTAC_A
Cuts at: 2244 2447 2244
Size: 203 5667
BssSI C'ACGA_G
Cuts at: 4141 4499 4141
Size: 358 5512
BstAPI GCAn_nnn'nTGC
Cuts at: 546 3247 546
Size: 2701 3169
BstBI TT'CG_AA
Cuts at: 2598 4231 2598
Size: 1633 4237
BstEII G'GTnAC_C
Cuts at: 1376 1376
Size: 5870
BstXI CCAn_nnnn'nTGG
Cuts at: 846 1441 2694 846
Size: 595 1253 4022
Fragments arranged by size:
4022 1253 595
BstYI r'GATC_y
Cuts at: 1 2822 2895 3362 3385 3720 3966 4978 5064
Size: 2821 73 467 23 335 246 1012 86
Cuts at: 5064 5076 5422 1
Size: 12 346 449
Fragments arranged by size:
2821 1012 467 449 346 335 246 86
73 23 12
Bsu36I CC'TnA_GG
Cuts at: 1565 1565
Size: 5870
BtgI C'CryG_G
Cuts at: 107 515 839 2351 107
Size: 408 324 1512 3626
Fragments arranged by size:
3626 1512 408 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3820 4001 4164 3820
Size: 181 163 5526
Fragments arranged by size:
5526 181 163
BtsI GCAGTG_nn'
Cuts at: 833 1321 2533 3113 833
Size: 488 1212 580 3590
Fragments arranged by size:
3590 1212 580 488
DraI TTT'AAA
Cuts at: 2869 2869
Size: 5870
DraIII CAC_nnn'GTG
Cuts at: 2766 2766
Size: 5870
DrdI GACnn_nn'nnGTC
Cuts at: 3576 4434 3576
Size: 858 5012
EaeI y'GGCC_r
Cuts at: 145 518 962 1214 3455 3629 4020 4047 5392
Size: 373 444 252 2241 174 391 27 1345
Cuts at: 5392 145
Size: 623
Fragments arranged by size:
2241 1345 623 444 391 373 252 174
27
EagI C'GGCC_G
Cuts at: 145 3455 145
Size: 3310 2560
EarI CTCTTCn'nnn_
Cuts at: 1420 2548 3893 4103 1420
Size: 1128 1345 210 3187
Fragments arranged by size:
3187 1345 1128 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1223 4078 4388 4534 5680 1223
Size: 2855 310 146 1146 1413
Fragments arranged by size:
2855 1413 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1106 1825 2882 1106
Size: 719 1057 4094
Fragments arranged by size:
4094 1057 719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 527 667 1031 1288 1889 2020 2367 2529
Size: 37 140 364 257 601 131 347 162
Cuts at: 2529 3695 4127 4874 5142 490
Size: 1166 432 747 268 1218
Fragments arranged by size:
1218 1166 747 601 432 364 347 268
257 162 140 131 37
EcoNI CCTnn'n_nnAGG
Cuts at: 433 658 433
Size: 225 5645
EcoO109I rG'GnC_Cy
Cuts at: 220 409 604 1329 1363 1678 3009 220
Size: 189 195 725 34 315 1331 3081
Fragments arranged by size:
3081 1331 725 315 195 189 34
EcoRI G'AATT_C
Cuts at: 2515 2874 2515
Size: 359 5511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1869 3889 1869
Size: 2020 3850
FspI TGC'GCA
Cuts at: 3651 3651
Size: 5870
HaeII r_GCGC'y
Cuts at: 263 1094 1295 3304 3552 4574 5553 263
Size: 831 201 2009 248 1022 979 580
Fragments arranged by size:
2009 1022 979 831 580 248 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 1681 1713 3712 3742 3744 3774 479
Size: 32 1170 32 1999 30 2 30 2575
Fragments arranged by size:
2575 1999 1170 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2379 2909 2379
Size: 530 5340
HindIII A'AGCT_T
Cuts at: 1802 1802
Size: 5870
KasI G'GCGC_C
Cuts at: 259 3300 3548 259
Size: 3041 248 2581
Fragments arranged by size:
3041 2581 248
KpnI G_GTAC'C
Cuts at: 1617 2890 1617
Size: 1273 4597
MluI A'CGCG_T
Cuts at: 5303 5303
Size: 5870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 842 4541 4725 842
Size: 3699 184 1987
Fragments arranged by size:
3699 1987 184
MscI TGG'CCA
Cuts at: 3631 3631
Size: 5870
MslI CAynn'nnrTG
Cuts at: 112 2806 3859 3986 112
Size: 2694 1053 127 1996
Fragments arranged by size:
2694 1996 1053 127
MspA1I CmG'CkG
Cuts at: 546 577 629 637 725 934 1171 1278 1360
Size: 31 52 8 88 209 237 107 82
Cuts at: 1360 1867 2981 3295 3655 4668 4913 546
Size: 507 1114 314 360 1013 245 1503
Fragments arranged by size:
1503 1114 1013 507 360 314 245 237
209 107 88 82 52 31 8
NaeI GCC'GGC
Cuts at: 4051 4051
Size: 5870
NarI GG'CG_CC
Cuts at: 260 3301 3549 260
Size: 3041 248 2581
Fragments arranged by size:
3041 2581 248
NcoI C'CATG_G
Cuts at: 107 839 2351 107
Size: 732 1512 3626
Fragments arranged by size:
3626 1512 732
NgoMIV G'CCGG_C
Cuts at: 4049 4049
Size: 5870
NruI TCG'CGA
Cuts at: 1187 1187
Size: 5870
NspI r_CATG'y
Cuts at: 906 1719 1835 2433 2639 2923 3423 4330 906
Size: 813 116 598 206 284 500 907 2446
Fragments arranged by size:
2446 907 813 598 500 284 206 116
PciI A'CATG_T
Cuts at: 1831 4326 1831
Size: 2495 3375
PflMI CCAn_nnn'nTGG
Cuts at: 505 2357 2563 505
Size: 1852 206 3812
Fragments arranged by size:
3812 1852 206
PfoI T'CCnGG_A
Cuts at: 428 1108 2135 2326 2383 2440 2693 428
Size: 680 1027 191 57 57 253 3605
Fragments arranged by size:
3605 1027 680 253 191 57 57
PmeI GTTT'AAAC
Cuts at: 2869 2869
Size: 5870
PmlI CAC'GTG
Cuts at: 2250 2295 2250
Size: 45 5825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5009 5041 5009
Size: 32 5838
PpuMI rG'GwC_Cy
Cuts at: 1329 1363 1329
Size: 34 5836
PspOMI G'GGCC_C
Cuts at: 219 603 1677 219
Size: 384 1074 4412
Fragments arranged by size:
4412 1074 384
PstI C_TGCA'G
Cuts at: 281 1098 2917 3602 281
Size: 817 1819 685 2549
Fragments arranged by size:
2549 1819 817 685
PvuI CG_AT'CG
Cuts at: 104 2625 104
Size: 2521 3349
PvuII CAG'CTG
Cuts at: 546 577 629 1171 3295 3655 546
Size: 31 52 542 2124 360 2761
Fragments arranged by size:
2761 2124 542 360 52 31
RsrII CG'GwC_CG
Cuts at: 1219 4065 1219
Size: 2846 3024
SacI G_AGCT'C
Cuts at: 1108 1827 2884 1108
Size: 719 1057 4094
Fragments arranged by size:
4094 1057 719
SalI G'TCGA_C
Cuts at: 2907 2907
Size: 5870
SanDI GG'GwC_CC
Cuts at: 1363 1363
Size: 5870
SapI GCTCTTCn'nnn_
Cuts at: 3893 4103 3893
Size: 210 5660
SbfI CC_TGCA'GG
Cuts at: 2917 2917
Size: 5870
ScaI AGT'ACT
Cuts at: 2323 5387 2323
Size: 3064 2806
SexAI A'CCwGG_T
Cuts at: 422 422
Size: 5870
SfcI C'TryA_G
Cuts at: 33 277 1094 1535 2023 2913 3598 4591 4782
Size: 244 817 441 488 890 685 993 191
Cuts at: 4782 33
Size: 1121
Fragments arranged by size:
1121 993 890 817 685 488 441 244
191
SfoI GGC'GCC
Cuts at: 261 3302 3550 261
Size: 3041 248 2581
Fragments arranged by size:
3041 2581 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5870
SmaI CCC'GGG
Cuts at: 218 2892 218
Size: 2674 3196
SmlI C'TyrA_G
Cuts at: 621 2480 2664 3025 3940 4432 4694 621
Size: 1859 184 361 915 492 262 1797
Fragments arranged by size:
1859 1797 915 492 361 262 184
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5870
SphI G_CATG'C
Cuts at: 2433 2639 2923 3423 2433
Size: 206 284 500 4880
Fragments arranged by size:
4880 500 284 206
StuI AGG'CCT
Cuts at: 2269 2269
Size: 5870
StyI C'CwwG_G
Cuts at: 107 764 809 839 1074 2264 2351 3004 3358
Size: 657 45 30 235 1190 87 653 354
Cuts at: 3358 107
Size: 2619
Fragments arranged by size:
2619 1190 657 653 354 235 87 45
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 245 838 1799 2875 3453 3586 4045 4254 245
Size: 593 961 1076 578 133 459 209 1861
Fragments arranged by size:
1861 1076 961 593 578 459 209 133
TatI w'GTAC_w
Cuts at: 2244 2321 2447 5385 2244
Size: 77 126 2938 2729
Fragments arranged by size:
2938 2729 126 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 2506 2538 5387 5419 500
Size: 32 1974 32 2849 32 951
Fragments arranged by size:
2849 1974 951 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2088 3667 2088
Size: 1579 4291
XbaI T'CTAG_A
Cuts at: 2901 2901
Size: 5870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 420 3365 420
Size: 2945 2925
XmaI C'CCGG_G
Cuts at: 216 2890 216
Size: 2674 3196
XmnI GAAnn'nnTTC
Cuts at: 184 887 184
Size: 703 5167
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI AscI BfrBI BmgBI
BmtI BsiWI BsmBI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI