Restriction map for
pF1KB0458
(Circular) (Six-base) MAPSORT of: pF1KB0458.seq Check: 5869 from: 1 to: 5960
pF1KB0458 5960 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1006 1006
Size: 5960
AccI GT'mk_AC
Cuts at: 2998 2998
Size: 5960
Acc65I G'GTAC_C
Cuts at: 2976 2976
Size: 5960
AclI AA'CG_TT
Cuts at: 5800 5800
Size: 5960
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 977 2032 2071 2761 3785 4217 4964 5232
Size: 60 1055 39 690 1024 432 747 268
Cuts at: 5232 917
Size: 1645
Fragments arranged by size:
1645 1055 1024 747 690 432 268 60
39
AflIII A'CryG_T
Cuts at: 266 1444 3312 4416 5393 266
Size: 1178 1868 1104 977 833
Fragments arranged by size:
1868 1178 1104 977 833
AgeI A'CCGG_T
Cuts at: 4348 4348
Size: 5960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 360 2496 360
Size: 2136 3824
AlwNI CAG_nnn'CTG
Cuts at: 799 3265 4832 799
Size: 2466 1567 1927
Fragments arranged by size:
2466 1927 1567
ApaLI G'TGCA_C
Cuts at: 2689 4730 2689
Size: 2041 3919
ApoI r'AATT_y
Cuts at: 987 1005 1135 1309 1824 2602 2773 2964 5313
Size: 18 130 174 515 778 171 191 2349
Cuts at: 5313 5569 987
Size: 256 1378
Fragments arranged by size:
2349 1378 778 515 256 191 174 171
130 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5960
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5960
AvaI C'yCGr_G
Cuts at: 408 1230 1624 2156 2980 408
Size: 822 394 532 824 3388
Fragments arranged by size:
3388 824 822 532 394
AvrII C'CTAG_G
Cuts at: 657 657
Size: 5960
BamHI G'GATC_C
Cuts at: 2354 2985 2354
Size: 631 5329
BanI G'GyrC_C
Cuts at: 492 1857 1966 2976 3390 3638 3673 492
Size: 1365 109 1010 414 248 35 2779
Fragments arranged by size:
2779 1365 1010 414 248 109 35
BanII G_rGCy'C
Cuts at: 1346 1586 2261 2344 2702 2974 4004 1346
Size: 240 675 83 358 272 1030 3302
Fragments arranged by size:
3302 1030 675 358 272 240 83
BbeI G_GCGC'C
Cuts at: 3394 3642 3394
Size: 248 5712
BbsI GAAGACnn'nnnn_
Cuts at: 2749 2749
Size: 5960
BciVI GTATCCnnnnn_n'
Cuts at: 3858 4619 5960 3858
Size: 761 1341 3858
Fragments arranged by size:
3858 1341 761
BclI T'GATC_A
Cuts at: 3480 3480
Size: 5960
BfrBI ATG'CAT
Cuts at: 2391 2391
Size: 5960
BglII A'GATC_T
Cuts at: 1026 1889 3475 5512 1026
Size: 863 1586 2037 1474
Fragments arranged by size:
2037 1586 1474 863
BlpI GC'TnA_GC
Cuts at: 3072 3072
Size: 5960
Bme1580I G_kGCm'C
Cuts at: 1862 2693 3585 3678 4734 1862
Size: 831 892 93 1056 3088
Fragments arranged by size:
3088 1056 892 831 93
BmgBI CAC'GTC
Cuts at: 267 2115 2124 267
Size: 1848 9 4103
Fragments arranged by size:
4103 1848 9
BmrI ACTGGGnnnn_n'
Cuts at: 1785 2904 2919 3172 3253 3588 5732 1785
Size: 1119 15 253 81 335 2144 2013
Fragments arranged by size:
2144 2013 1119 335 253 81 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 171 4327 171
Size: 4156 1804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2686 2686
Size: 5960
Bpu10I CC'TnA_GC
Cuts at: 1585 1585
Size: 5960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2578 3136 4015 4507 4805 1360
Size: 1218 558 879 492 298 2515
Fragments arranged by size:
2515 1218 879 558 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 515 2354 5664 154
Size: 361 1839 3310 450
Fragments arranged by size:
3310 1839 450 361
BsaAI yAC'GTr
Cuts at: 1720 3313 1720
Size: 1593 4367
BsaBI GATnn'nnATC
Cuts at: 6 3474 5511 6
Size: 3468 2037 455
Fragments arranged by size:
3468 2037 455
BsaHI Gr'CG_yC
Cuts at: 1497 3391 3639 4341 5837 1497
Size: 1894 248 702 1496 1620
Fragments arranged by size:
1894 1620 1496 702 248
BsaWI w'CCGG_w
Cuts at: 552 2076 3149 3670 4348 4622 4769 5180 552
Size: 1524 1073 521 678 274 147 411 1332
Fragments arranged by size:
1524 1332 1073 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1396 1426 2227 2257 1396
Size: 30 801 30 5099
Fragments arranged by size:
5099 801 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 1293 2710 144
Size: 1149 1417 3394
Fragments arranged by size:
3394 1417 1149
BseYI C'CCAG_C
Cuts at: 675 1696 1971 3385 4143 4720 5216 675
Size: 1021 275 1414 758 577 496 1419
Fragments arranged by size:
1419 1414 1021 758 577 496 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1696 562
Size: 1134 4826
BsiEI CG_ry'CG
Cuts at: 104 3502 3548 4756 104
Size: 3398 46 1208 1308
Fragments arranged by size:
3398 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1346 2094 2344 2693 2702 2851 2974 3752 3942
Size: 748 250 349 9 149 123 778 190
Cuts at: 3942 4734 1346
Size: 792 2572
Fragments arranged by size:
2572 792 778 748 349 250 190 149
123 9
BsmI GAATG_Cn'
Cuts at: 1553 1553
Size: 5960
Bsp1286I G_dGCh'C
Cuts at: 1346 1586 1862 2094 2261 2344 2693 2702 2851
Size: 240 276 232 167 83 349 9 149
Cuts at: 2851 2974 3585 3678 3752 3942 4004 4734 1346
Size: 123 611 93 74 190 62 730 2572
Fragments arranged by size:
2572 730 611 349 276 240 232 190
167 149 123 93 83 74 62 9
BspEI T'CCGG_A
Cuts at: 552 2076 5180 552
Size: 1524 3104 1332
Fragments arranged by size:
3104 1524 1332
BspHI T'CATG_A
Cuts at: 5136 5136
Size: 5960
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 1006 2729 3010 3224 3526 3907 390
Size: 616 1723 281 214 302 381 2443
Fragments arranged by size:
2443 1723 616 381 302 281 214
BsrBI CCG'CTC
Cuts at: 50 4252 4306 5790 5955 50
Size: 4202 54 1484 165 55
Fragments arranged by size:
4202 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 885 2731 2817 3872 885
Size: 1846 86 1055 2973
Fragments arranged by size:
2973 1846 1055 86
BsrFI r'CCGG_y
Cuts at: 3958 4139 4348 3958
Size: 181 209 5570
Fragments arranged by size:
5570 209 181
BssSI C'ACGA_G
Cuts at: 184 1567 2260 2692 4231 4589 184
Size: 1383 693 432 1539 358 1555
Fragments arranged by size:
1555 1539 1383 693 432 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3337 3337
Size: 5960
BstBI TT'CG_AA
Cuts at: 2279 4321 2279
Size: 2042 3918
BstXI CCAn_nnnn'nTGG
Cuts at: 171 924 1794 171
Size: 753 870 4337
Fragments arranged by size:
4337 870 753
BstYI r'GATC_y
Cuts at: 1 709 825 1026 1283 1889 2354 2985 3452
Size: 708 116 201 257 606 465 631 467
Cuts at: 3452 3475 3810 4056 5068 5154 5166 5512 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 708 631 606 467 465 449 346
335 257 246 201 116 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 474 474
Size: 5960
BtgI C'CryG_G
Cuts at: 107 764 1809 107
Size: 657 1045 4258
Fragments arranged by size:
4258 1045 657
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 231 1990 2573 2931 3910 4091 4254 231
Size: 1759 583 358 979 181 163 1937
Fragments arranged by size:
1937 1759 979 583 358 181 163
BtsI GCAGTG_nn'
Cuts at: 128 257 443 3203 128
Size: 129 186 2760 2885
Fragments arranged by size:
2885 2760 186 129
ClaI AT'CG_AT
Cuts at: 1760 1760
Size: 5960
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1136 1171 1381 1416 1136
Size: 35 210 35 5680
Fragments arranged by size:
5680 210 35 35
DraI TTT'AAA
Cuts at: 2959 2959
Size: 5960
DraIII CAC_nnn'GTG
Cuts at: 727 1619 2089 727
Size: 892 470 4598
Fragments arranged by size:
4598 892 470
DrdI GACnn_nn'nnGTC
Cuts at: 271 668 3666 4524 271
Size: 397 2998 858 1707
Fragments arranged by size:
2998 1707 858 397
EaeI y'GGCC_r
Cuts at: 218 1796 3545 3719 4110 4137 5482 218
Size: 1578 1749 174 391 27 1345 696
Fragments arranged by size:
1749 1578 1345 696 391 174 27
EagI C'GGCC_G
Cuts at: 3545 3545
Size: 5960
EarI CTCTTCn'nnn_
Cuts at: 140 1903 3983 4193 140
Size: 1763 2080 210 1907
Fragments arranged by size:
2080 1907 1763 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4168 4478 4624 5770 4168
Size: 310 146 1146 4358
Fragments arranged by size:
4358 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1344 2342 2700 2972 1344
Size: 998 358 272 4332
Fragments arranged by size:
4332 998 358 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 977 2032 2071 2686 2761 3785 4217 4964
Size: 60 1055 39 615 75 1024 432 747
Cuts at: 4964 5232 917
Size: 268 1645
Fragments arranged by size:
1645 1055 1024 747 615 432 268 75
60 39
EcoO109I rG'GnC_Cy
Cuts at: 951 1248 1689 3099 951
Size: 297 441 1410 3812
Fragments arranged by size:
3812 1410 441 297
EcoRI G'AATT_C
Cuts at: 2602 2773 2964 2602
Size: 171 191 5598
Fragments arranged by size:
5598 191 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 881 1614 3979 881
Size: 733 2365 2862
Fragments arranged by size:
2862 2365 733
FspI TGC'GCA
Cuts at: 2823 3741 2823
Size: 918 5042
HaeII r_GCGC'y
Cuts at: 3394 3642 4664 5643 3394
Size: 248 1022 979 3711
Fragments arranged by size:
3711 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2376 2408 2496 2528 3802 3832 3834 3864 2376
Size: 32 88 32 1274 30 2 30 4472
Fragments arranged by size:
4472 1274 88 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2379 2999 2379
Size: 620 5340
HindIII A'AGCT_T
Cuts at: 1561 1561
Size: 5960
KasI G'GCGC_C
Cuts at: 3390 3638 3390
Size: 248 5712
KpnI G_GTAC'C
Cuts at: 2980 2980
Size: 5960
MfeI C'AATT_G
Cuts at: 1148 1148
Size: 5960
MluI A'CGCG_T
Cuts at: 5393 5393
Size: 5960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 258 1465 1681 4631 4815 258
Size: 1207 216 2950 184 1403
Fragments arranged by size:
2950 1403 1207 216 184
MscI TGG'CCA
Cuts at: 220 1798 3721 220
Size: 1578 1923 2459
Fragments arranged by size:
2459 1923 1578
MslI CAynn'nnrTG
Cuts at: 250 748 1471 2122 2386 2443 3949 4076 250
Size: 498 723 651 264 57 1506 127 2134
Fragments arranged by size:
2134 1506 723 651 498 264 127 57
MspA1I CmG'CkG
Cuts at: 675 924 1297 2868 3071 3385 3745 4758 5003
Size: 249 373 1571 203 314 360 1013 245
Cuts at: 5003 675
Size: 1632
Fragments arranged by size:
1632 1571 1013 373 360 314 249 245
203
NaeI GCC'GGC
Cuts at: 4141 4141
Size: 5960
NarI GG'CG_CC
Cuts at: 3391 3639 3391
Size: 248 5712
NcoI C'CATG_G
Cuts at: 107 764 107
Size: 657 5303
NdeI CA'TA_TG
Cuts at: 1453 1453
Size: 5960
NgoMIV G'CCGG_C
Cuts at: 4139 4139
Size: 5960
NsiI A_TGCA'T
Cuts at: 2393 2393
Size: 5960
NspI r_CATG'y
Cuts at: 1227 1448 1470 3013 3513 4420 1227
Size: 221 22 1543 500 907 2767
Fragments arranged by size:
2767 1543 907 500 221 22
PasI CC'CwG_GG
Cuts at: 685 685
Size: 5960
PciI A'CATG_T
Cuts at: 1444 4416 1444
Size: 2972 2988
PflMI CCAn_nnn'nTGG
Cuts at: 1539 2118 2351 1539
Size: 579 233 5148
Fragments arranged by size:
5148 579 233
PmeI GTTT'AAAC
Cuts at: 2959 2959
Size: 5960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5099 5131 5099
Size: 32 5928
PpuMI rG'GwC_Cy
Cuts at: 951 1689 951
Size: 738 5222
PstI C_TGCA'G
Cuts at: 1014 3007 3692 1014
Size: 1993 685 3282
Fragments arranged by size:
3282 1993 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5960
PvuII CAG'CTG
Cuts at: 675 1297 2868 3385 3745 675
Size: 622 1571 517 360 2890
Fragments arranged by size:
2890 1571 622 517 360
RsrII CG'GwC_CG
Cuts at: 4155 4155
Size: 5960
SacI G_AGCT'C
Cuts at: 1346 2344 2702 2974 1346
Size: 998 358 272 4332
Fragments arranged by size:
4332 998 358 272
SalI G'TCGA_C
Cuts at: 2997 2997
Size: 5960
SapI GCTCTTCn'nnn_
Cuts at: 1903 3983 4193 1903
Size: 2080 210 3670
Fragments arranged by size:
3670 2080 210
SbfI CC_TGCA'GG
Cuts at: 3007 3007
Size: 5960
ScaI AGT'ACT
Cuts at: 5477 5477
Size: 5960
SfcI C'TryA_G
Cuts at: 33 1010 1179 1682 3003 3688 4681 4872 33
Size: 977 169 503 1321 685 993 191 1121
Fragments arranged by size:
1321 1121 993 977 685 503 191 169
SfoI GGC'GCC
Cuts at: 3392 3640 3392
Size: 248 5712
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5960
SmaI CCC'GGG
Cuts at: 1232 2158 2982 1232
Size: 926 824 4210
Fragments arranged by size:
4210 926 824
SmlI C'TyrA_G
Cuts at: 1339 2593 3115 4030 4522 4784 1339
Size: 1254 522 915 492 262 2515
Fragments arranged by size:
2515 1254 915 522 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5960
SphI G_CATG'C
Cuts at: 1227 3013 3513 1227
Size: 1786 500 3674
Fragments arranged by size:
3674 1786 500
SspI AAT'ATT
Cuts at: 1437 1437
Size: 5960
StyI C'CwwG_G
Cuts at: 107 227 657 764 2285 2418 3094 3448 107
Size: 120 430 107 1521 133 676 354 2619
Fragments arranged by size:
2619 1521 676 430 354 133 120 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 667 1804 2614 3543 3676 4135 4344 667
Size: 1137 810 929 133 459 209 2283
Fragments arranged by size:
2283 1137 929 810 459 209 133
TatI w'GTAC_w
Cuts at: 1428 2225 2937 5475 1428
Size: 797 712 2538 1913
Fragments arranged by size:
2538 1913 797 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 853 885 1605 1637 2227 2259 2911 2943 5477
Size: 32 720 32 590 32 652 32 2534
Cuts at: 5477 5509 853
Size: 32 1304
Fragments arranged by size:
2534 1304 720 652 590 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 3757 2113
Size: 1644 4316
XbaI T'CTAG_A
Cuts at: 2991 2991
Size: 5960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 2535 3455 234
Size: 2301 920 2739
Fragments arranged by size:
2739 2301 920
XmaI C'CCGG_G
Cuts at: 1230 2156 2980 1230
Size: 926 824 4210
Fragments arranged by size:
4210 926 824
XmnI GAAnn'nnTTC
Cuts at: 1844 1921 1844
Size: 77 5883
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BaeI BbvCI BcgI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I EcoNI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI