Restriction map for
pF1KB0459
(Circular) (Six-base) MAPSORT of: pF1KB0459.seq Check: 8846 from: 1 to: 4637
pF1KB0459 4637 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1555 1555
Size: 4637
AatII G_ACGT'C
Cuts at: 608 608
Size: 4637
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 368 1430 1653 368
Size: 1062 223 3352
Fragments arranged by size:
3352 1062 223
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2894 3641 3909 2462
Size: 432 747 268 3190
Fragments arranged by size:
3190 747 432 268
AflIII A'CryG_T
Cuts at: 1989 3093 4070 1989
Size: 1104 977 2556
Fragments arranged by size:
2556 1104 977
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AhdI GACnn_n'nnGTC
Cuts at: 164 1067 164
Size: 903 3734
AleI CACnn'nnGTG
Cuts at: 389 389
Size: 4637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 523
Size: 32 4605
AlwNI CAG_nnn'CTG
Cuts at: 180 516 1008 1098 1490 1942 3509 180
Size: 336 492 90 392 452 1567 1308
Fragments arranged by size:
1567 1308 492 452 392 336 90
ApaI G_GGCC'C
Cuts at: 255 255
Size: 4637
ApaLI G'TGCA_C
Cuts at: 1156 1468 3407 1156
Size: 312 1939 2386
Fragments arranged by size:
2386 1939 312
ApoI r'AATT_y
Cuts at: 1641 3990 4246 1641
Size: 2349 256 2032
Fragments arranged by size:
2349 2032 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 255 492 526 1064 1288 1447 1547 1657 255
Size: 237 34 538 224 159 100 110 3235
Fragments arranged by size:
3235 538 237 224 159 110 100 34
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 209 259 368 1284 1430 1567 1653 2067 2315
Size: 50 109 916 146 137 86 414 248
Cuts at: 2315 2350 209
Size: 35 2496
Fragments arranged by size:
2496 916 414 248 146 137 109 86
50 35
BanII G_rGCy'C
Cuts at: 190 255 1077 1380 1505 1631 1651 2681 190
Size: 65 822 303 125 126 20 1030 2146
Fragments arranged by size:
2146 1030 822 303 126 125 65 20
BbeI G_GCGC'C
Cuts at: 1288 2071 2319 1288
Size: 783 248 3606
Fragments arranged by size:
3606 783 248
BbsI GAAGACnn'nnnn_
Cuts at: 1353 1353
Size: 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 2157 2157
Size: 4637
BglI GCCn_nnn'nGGC
Cuts at: 243 569 243
Size: 326 4311
BglII A'GATC_T
Cuts at: 488 2152 4189 488
Size: 1664 2037 936
Fragments arranged by size:
2037 1664 936
BlpI GC'TnA_GC
Cuts at: 460 1091 1749 460
Size: 631 658 3348
Fragments arranged by size:
3348 658 631
Bme1580I G_kGCm'C
Cuts at: 255 262 1160 1472 1572 2262 2355 3411 255
Size: 7 898 312 100 690 93 1056 1481
Fragments arranged by size:
1481 1056 898 690 312 100 93 7
BmgBI CAC'GTC
Cuts at: 330 1473 330
Size: 1143 3494
BmrI ACTGGGnnnn_n'
Cuts at: 258 1849 1930 2265 4409 258
Size: 1591 81 335 2144 486
Fragments arranged by size:
2144 1591 486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 459 1380 3004 459
Size: 921 1624 2092
Fragments arranged by size:
2092 1624 921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1159 1353 364
Size: 795 194 3648
Fragments arranged by size:
3648 795 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1242 1813 2692 3184 3482 1242
Size: 571 879 492 298 2397
Fragments arranged by size:
2397 879 571 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 729 1194 4341 729
Size: 465 3147 1025
Fragments arranged by size:
3147 1025 465
BsaAI yAC'GTr
Cuts at: 1029 1116 1990 1029
Size: 87 874 3676
Fragments arranged by size:
3676 874 87
BsaBI GATnn'nnATC
Cuts at: 6 2151 4188 6
Size: 2145 2037 455
Fragments arranged by size:
2145 2037 455
BsaHI Gr'CG_yC
Cuts at: 605 1285 1595 2068 2316 3018 4514 605
Size: 680 310 473 248 702 1496 728
Fragments arranged by size:
1496 728 702 680 473 310 248
BsaWI w'CCGG_w
Cuts at: 1826 2347 3025 3299 3446 3857 1826
Size: 521 678 274 147 411 2606
Fragments arranged by size:
2606 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1414 1444 1414
Size: 30 4607
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 787 784
Size: 3 4634
BseYI C'CCAG_C
Cuts at: 248 415 1490 1572 2062 2820 3397 3893 248
Size: 167 1075 82 490 758 577 496 992
Fragments arranged by size:
1075 992 758 577 496 490 167 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 705 982 1096 1583 610
Size: 95 277 114 487 3664
Fragments arranged by size:
3664 487 277 114 95
BsiEI CG_ry'CG
Cuts at: 104 767 944 2179 2225 3433 104
Size: 663 177 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 663 177 46
BsiHKAI G_wGCw'C
Cuts at: 287 828 1028 1160 1472 1651 2429 2619 3411
Size: 541 200 132 312 179 778 190 792
Cuts at: 3411 287
Size: 1513
Fragments arranged by size:
1513 792 778 541 312 200 190 179
132
BsmBI CGTCTCn'nnnn_
Cuts at: 228 931 985 1587 228
Size: 703 54 602 3278
Fragments arranged by size:
3278 703 602 54
Bsp1286I G_dGCh'C
Cuts at: 190 255 262 287 828 1028 1077 1160 1380
Size: 65 7 25 541 200 49 83 220
Cuts at: 1380 1472 1505 1572 1631 1651 2262 2355 2429
Size: 92 33 67 59 20 611 93 74
Cuts at: 2429 2619 2681 3411 190
Size: 190 62 730 1416
Fragments arranged by size:
1416 730 611 541 220 200 190 93
92 83 74 67 65 62 59 49
33 25 20 7
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 947 1478 3813 947
Size: 531 2335 1771
Fragments arranged by size:
2335 1771 531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 812 1555 1687 1901 2203 2584 812
Size: 743 132 214 302 381 2865
Fragments arranged by size:
2865 743 381 302 214 132
BsrBI CCG'CTC
Cuts at: 50 432 1090 1242 2929 2983 4467 4632 50
Size: 382 658 152 1687 54 1484 165 55
Fragments arranged by size:
1687 1484 658 382 165 152 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 1262 1511 2635 2816 3025 1262
Size: 249 1124 181 209 2874
Fragments arranged by size:
2874 1124 249 209 181
BsrGI T'GTAC_A
Cuts at: 1010 1010
Size: 4637
BssHII G'CGCG_C
Cuts at: 559 559
Size: 4637
BssSI C'ACGA_G
Cuts at: 964 1078 2908 3266 964
Size: 114 1830 358 2335
Fragments arranged by size:
2335 1830 358 114
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 4637
BstXI CCAn_nnnn'nTGG
Cuts at: 391 391
Size: 4637
BstYI r'GATC_y
Cuts at: 1 488 1662 2129 2152 2487 2733 3745 3831
Size: 487 1174 467 23 335 246 1012 86
Cuts at: 3831 3843 4189 1
Size: 12 346 449
Fragments arranged by size:
1174 1012 487 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 162 472 480 1418 162
Size: 310 8 938 3381
Fragments arranged by size:
3381 938 310 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 1566 1880 1566
Size: 314 4323
ClaI AT'CG_AT
Cuts at: 650 650
Size: 4637
DraI TTT'AAA
Cuts at: 1636 1636
Size: 4637
DraIII CAC_nnn'GTG
Cuts at: 266 266
Size: 4637
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 597 636 764 779 887 941 1118 1169 1205
Size: 39 128 15 108 54 177 51 36
Cuts at: 1205 1229 1513 1532 2222 2396 2787 2814 4159
Size: 24 284 19 690 174 391 27 1345
Cuts at: 4159 597
Size: 1075
Fragments arranged by size:
1345 1075 690 391 284 177 174 128
108 54 51 39 36 27 24 19
15
EagI C'GGCC_G
Cuts at: 764 941 2222 764
Size: 177 1281 3179
Fragments arranged by size:
3179 1281 177
EarI CTCTTCn'nnn_
Cuts at: 129 2660 2870 129
Size: 2531 210 1896
Fragments arranged by size:
2531 1896 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1649 1649
Size: 4637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1159 1353 2462 2894 3641 3909 364
Size: 795 194 1109 432 747 268 1092
Fragments arranged by size:
1109 1092 795 747 432 268 194
EcoNI CCTnn'n_nnAGG
Cuts at: 152 1000 152
Size: 848 3789
EcoO109I rG'GnC_Cy
Cuts at: 158 252 438 1197 1462 1776 158
Size: 94 186 759 265 314 3019
Fragments arranged by size:
3019 759 314 265 186 94
EcoRI G'AATT_C
Cuts at: 1641 1641
Size: 4637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4637
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 249 424 716 1288 2071 2319 3341 4320 249
Size: 175 292 572 783 248 1022 979 566
Fragments arranged by size:
1022 979 783 572 566 292 248 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 150 182 815 847 1053 1085 2479 2509 2511
Size: 32 633 32 206 32 1394 30 2
Cuts at: 2511 2541 150
Size: 30 2246
Fragments arranged by size:
2246 1394 633 206 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1676 1676
Size: 4637
HindIII A'AGCT_T
Cuts at: 1150 1150
Size: 4637
KasI G'GCGC_C
Cuts at: 1284 2067 2315 1284
Size: 783 248 3606
Fragments arranged by size:
3606 783 248
KpnI G_GTAC'C
Cuts at: 372 1434 1657 372
Size: 1062 223 3352
Fragments arranged by size:
3352 1062 223
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1391 3308 3492 1304
Size: 87 1917 184 2449
Fragments arranged by size:
2449 1917 184 87
MscI TGG'CCA
Cuts at: 781 1207 1231 2398 781
Size: 426 24 1167 3020
Fragments arranged by size:
3020 1167 426 24
MslI CAynn'nnrTG
Cuts at: 389 682 1018 2626 2753 389
Size: 293 336 1608 127 2273
Fragments arranged by size:
2273 1608 336 293 127
MspA1I CmG'CkG
Cuts at: 415 516 1487 1748 2062 2422 3435 3680 415
Size: 101 971 261 314 360 1013 245 1372
Fragments arranged by size:
1372 1013 971 360 314 261 245 101
NaeI GCC'GGC
Cuts at: 1264 1513 2818 1264
Size: 249 1305 3083
Fragments arranged by size:
3083 1305 249
NarI GG'CG_CC
Cuts at: 1285 2068 2316 1285
Size: 783 248 3606
Fragments arranged by size:
3606 783 248
NdeI CA'TA_TG
Cuts at: 1368 1368
Size: 4637
NgoMIV G'CCGG_C
Cuts at: 1262 1511 2816 1262
Size: 249 1305 3083
Fragments arranged by size:
3083 1305 249
NspI r_CATG'y
Cuts at: 1690 2190 3097 1690
Size: 500 907 3230
Fragments arranged by size:
3230 907 500
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PfoI T'CCnGG_A
Cuts at: 341 929 341
Size: 588 4049
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PmlI CAC'GTG
Cuts at: 1029 1116 1029
Size: 87 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 646 678 3776 3808 646
Size: 32 3098 32 1475
Fragments arranged by size:
3098 1475 32 32
PpuMI rG'GwC_Cy
Cuts at: 158 1197 158
Size: 1039 3598
PshAI GACnn'nnGTC
Cuts at: 943 943
Size: 4637
PspOMI G'GGCC_C
Cuts at: 251 251
Size: 4637
PstI C_TGCA'G
Cuts at: 521 809 863 1103 1684 2369 521
Size: 288 54 240 581 685 2789
Fragments arranged by size:
2789 685 581 288 240 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 415 516 1487 2062 2422 415
Size: 101 971 575 360 2630
Fragments arranged by size:
2630 971 575 360 101
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 1651 1651
Size: 4637
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SanDI GG'GwC_CC
Cuts at: 158 158
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 2660 2870 2660
Size: 210 4427
SbfI CC_TGCA'GG
Cuts at: 863 1684 863
Size: 821 3816
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SexAI A'CCwGG_T
Cuts at: 971 1043 1199 971
Size: 72 156 4409
Fragments arranged by size:
4409 156 72
SfcI C'TryA_G
Cuts at: 33 517 805 859 1099 1680 2365 3358 3549
Size: 484 288 54 240 581 685 993 191
Cuts at: 3549 33
Size: 1121
Fragments arranged by size:
1121 993 685 581 484 288 240 191
54
SfoI GGC'GCC
Cuts at: 1286 2069 2317 1286
Size: 783 248 3606
Fragments arranged by size:
3606 783 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 257 1066 1549 1659 257
Size: 809 483 110 3235
Fragments arranged by size:
3235 809 483 110
SmlI C'TyrA_G
Cuts at: 1257 1792 2707 3199 3461 1257
Size: 535 915 492 262 2433
Fragments arranged by size:
2433 915 535 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1690 2190 1690
Size: 500 4137
StuI AGG'CCT
Cuts at: 349 925 349
Size: 576 4061
StyI C'CwwG_G
Cuts at: 1208 1771 2125 1208
Size: 563 354 3720
Fragments arranged by size:
3720 563 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 2220 2353 2812 3021 277
Size: 1943 133 459 209 1893
Fragments arranged by size:
1943 1893 459 209 133
TatI w'GTAC_w
Cuts at: 1010 4152 1010
Size: 3142 1495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 331 577 759 2434 331
Size: 246 182 1675 2534
Fragments arranged by size:
2534 1675 246 182
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 245 2132 245
Size: 1887 2750
XmaI C'CCGG_G
Cuts at: 255 1064 1547 1657 255
Size: 809 483 110 3235
Fragments arranged by size:
3235 809 483 110
ZraI GAC'GTC
Cuts at: 606 606
Size: 4637
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AscI AvrII BaeI BbvCI BfrBI
BmtI Bpu10I BsiWI BsmI BstZ17I Bsu36I CspCI EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PflMI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI