Restriction map for
pF1KB0463
(Circular) (Six-base) MAPSORT of: pF1KB0463.seq Check: 786 from: 1 to: 4640
pF1KB0463 4640 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 617 617
Size: 4640
AccI GT'mk_AC
Cuts at: 1678 1678
Size: 4640
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 907 1323 2465 2897 3644 3912 859
Size: 48 416 1142 432 747 268 1587
Fragments arranged by size:
1587 1142 747 432 416 268 48
AflII C'TTAA_G
Cuts at: 1503 1503
Size: 4640
AflIII A'CryG_T
Cuts at: 1992 3096 4073 1992
Size: 1104 977 2559
Fragments arranged by size:
2559 1104 977
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 675 942 969 1945 3512 675
Size: 267 27 976 1567 1803
Fragments arranged by size:
1803 1567 976 267 27
ApaI G_GGCC'C
Cuts at: 354 1448 354
Size: 1094 3546
ApaLI G'TGCA_C
Cuts at: 3410 3410
Size: 4640
ApoI r'AATT_y
Cuts at: 1644 3993 4249 1644
Size: 2349 256 2035
Fragments arranged by size:
2349 2035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 778 814 1520 1660 778
Size: 36 706 140 3758
Fragments arranged by size:
3758 706 140 36
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 331 544 573 616 1151 1656 2070 2318 2353
Size: 213 29 43 535 505 414 248 35
Cuts at: 2353 331
Size: 2618
Fragments arranged by size:
2618 535 505 414 248 213 43 35
29
BanII G_rGCy'C
Cuts at: 354 787 1337 1448 1654 2684 354
Size: 433 550 111 206 1030 2310
Fragments arranged by size:
2310 1030 550 433 206 111
BbeI G_GCGC'C
Cuts at: 548 620 1155 2074 2322 548
Size: 72 535 919 248 2866
Fragments arranged by size:
2866 919 535 248 72
BbsI GAAGACnn'nnnn_
Cuts at: 1104 1446 1104
Size: 342 4298
BbvCI CC'TCA_GC
Cuts at: 155 155
Size: 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 645 679 805 839 90
Size: 34 521 34 126 34 3891
Fragments arranged by size:
3891 521 126 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2538 3299 4640 2538
Size: 761 1341 2538
Fragments arranged by size:
2538 1341 761
BclI T'GATC_A
Cuts at: 971 2160 971
Size: 1189 3451
BglI GCCn_nnn'nGGC
Cuts at: 485 851 1181 485
Size: 366 330 3944
Fragments arranged by size:
3944 366 330
BglII A'GATC_T
Cuts at: 1541 2155 4192 1541
Size: 614 2037 1989
Fragments arranged by size:
2037 1989 614
BlpI GC'TnA_GC
Cuts at: 881 994 1752 881
Size: 113 758 3769
Fragments arranged by size:
3769 758 113
Bme1580I G_kGCm'C
Cuts at: 354 578 700 1448 2265 2358 3414 354
Size: 224 122 748 817 93 1056 1580
Fragments arranged by size:
1580 1056 817 748 224 122 93
BmgBI CAC'GTC
Cuts at: 284 284
Size: 4640
BmrI ACTGGGnnnn_n'
Cuts at: 179 1852 1933 2268 4412 179
Size: 1673 81 335 2144 407
Fragments arranged by size:
2144 1673 407 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 890 919 928 1249 311
Size: 579 29 9 321 3702
Fragments arranged by size:
3702 579 321 29 9
Bpu10I CC'TnA_GC
Cuts at: 155 155
Size: 4640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 1816 2695 3187 3485 1627
Size: 189 879 492 298 2782
Fragments arranged by size:
2782 879 492 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 1128 4344 1128
Size: 3216 1424
BsaAI yAC'GTr
Cuts at: 585 1993 585
Size: 1408 3232
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 545 617 1152 2071 2319 3021 4517 545
Size: 72 535 919 248 702 1496 668
Fragments arranged by size:
1496 919 702 668 535 248 72
BsaWI w'CCGG_w
Cuts at: 1559 1829 2350 3028 3302 3449 3860 1559
Size: 270 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 731 761 1082 1112 731
Size: 30 321 30 4259
Fragments arranged by size:
4259 321 30 30
BseYI C'CCAG_C
Cuts at: 288 313 468 675 854 1479 1594 2065 2823
Size: 25 155 207 179 625 115 471 758
Cuts at: 2823 3400 3896 288
Size: 577 496 1032
Fragments arranged by size:
1032 758 625 577 496 471 207 179
155 115 25
BsiEI CG_ry'CG
Cuts at: 104 983 2182 2228 3436 104
Size: 879 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 879 46
BsiHKAI G_wGCw'C
Cuts at: 1337 1654 2432 2622 3414 1337
Size: 317 778 190 792 2563
Fragments arranged by size:
2563 792 778 317 190
BsmI GAATG_Cn'
Cuts at: 656 656
Size: 4640
BsmBI CGTCTCn'nnnn_
Cuts at: 274 1260 274
Size: 986 3654
Bsp1286I G_dGCh'C
Cuts at: 354 578 700 787 1337 1448 1654 2265 2358
Size: 224 122 87 550 111 206 611 93
Cuts at: 2358 2432 2622 2684 3414 354
Size: 74 190 62 730 1580
Fragments arranged by size:
1580 730 611 550 224 206 190 122
111 93 87 74 62
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 3816 3816
Size: 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 617 750 1690 1904 2206 2587 617
Size: 133 940 214 302 381 2670
Fragments arranged by size:
2670 940 381 302 214 133
BsrBI CCG'CTC
Cuts at: 50 918 1143 2932 2986 4470 4635 50
Size: 868 225 1789 54 1484 165 55
Fragments arranged by size:
1789 1484 868 225 165 55 54
BsrDI GCAATG_nn'
Cuts at: 974 2552 974
Size: 1578 3062
BsrFI r'CCGG_y
Cuts at: 959 2638 2819 3028 959
Size: 1679 181 209 2571
Fragments arranged by size:
2571 1679 209 181
BssSI C'ACGA_G
Cuts at: 2911 3269 2911
Size: 358 4282
BstAPI GCAn_nnn'nTGC
Cuts at: 2017 2017
Size: 4640
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstXI CCAn_nnnn'nTGG
Cuts at: 585 1371 585
Size: 786 3854
BstYI r'GATC_y
Cuts at: 1 1541 1665 2132 2155 2490 2736 3748 3834
Size: 1540 124 467 23 335 246 1012 86
Cuts at: 3834 3846 4192 1
Size: 12 346 449
Fragments arranged by size:
1540 1012 467 449 346 335 246 124
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 305 305
Size: 4640
BtgI C'CryG_G
Cuts at: 359 374 1364 359
Size: 15 990 3635
Fragments arranged by size:
3635 990 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 698 715 1883 698
Size: 17 1168 3455
Fragments arranged by size:
3455 1168 17
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 2346 3204 2346
Size: 858 3782
EaeI y'GGCC_r
Cuts at: 362 491 641 712 919 1265 1328 2225 2399
Size: 129 150 71 207 346 63 897 174
Cuts at: 2399 2790 2817 4162 362
Size: 391 27 1345 840
Fragments arranged by size:
1345 897 840 391 346 207 174 150
129 71 63 27
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 1342 2663 2873 1342
Size: 1321 210 3109
Fragments arranged by size:
3109 1321 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2848 3158 3304 4450 2848
Size: 310 146 1146 3038
Fragments arranged by size:
3038 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1335 1652 1335
Size: 317 4323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 859 890 907 919 928 1249 1323 2465
Size: 548 31 17 12 9 321 74 1142
Cuts at: 2465 2897 3644 3912 311
Size: 432 747 268 1039
Fragments arranged by size:
1142 1039 747 548 432 321 268 74
31 17 12 9
EcoO109I rG'GnC_Cy
Cuts at: 173 301 310 351 820 1348 1444 1445 1779
Size: 128 9 41 469 528 96 1 334
Cuts at: 1779 173
Size: 3034
Fragments arranged by size:
3034 528 469 334 128 96 41 9
1
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4640
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 548 620 846 1155 2074 2322 3344 4323 548
Size: 72 226 309 919 248 1022 979 865
Fragments arranged by size:
1022 979 919 865 309 248 226 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 521 553 1149 1181 1200 1232 1528 1554 1560
Size: 32 596 32 19 32 296 26 6
Cuts at: 1560 1586 2482 2512 2514 2544 521
Size: 26 896 30 2 30 2617
Fragments arranged by size:
2617 896 596 296 32 32 32 30
30 26 26 19 6 2
HincII GTy'rAC
Cuts at: 450 1104 1679 450
Size: 654 575 3411
Fragments arranged by size:
3411 654 575
KasI G'GCGC_C
Cuts at: 544 616 1151 2070 2318 544
Size: 72 535 919 248 2866
Fragments arranged by size:
2866 919 535 248 72
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 147 1409 3311 3495 147
Size: 1262 1902 184 1292
Fragments arranged by size:
1902 1292 1262 184
MscI TGG'CCA
Cuts at: 364 493 643 714 1267 2401 364
Size: 129 150 71 553 1134 2603
Fragments arranged by size:
2603 1134 553 150 129 71
MslI CAynn'nnrTG
Cuts at: 583 1204 1369 1411 2629 2756 583
Size: 621 165 42 1218 127 2467
Fragments arranged by size:
2467 1218 621 165 127 42
MspA1I CmG'CkG
Cuts at: 756 993 1549 1751 2065 2425 3438 3683 756
Size: 237 556 202 314 360 1013 245 1713
Fragments arranged by size:
1713 1013 556 360 314 245 237 202
NaeI GCC'GGC
Cuts at: 2821 2821
Size: 4640
NarI GG'CG_CC
Cuts at: 545 617 1152 2071 2319 545
Size: 72 535 919 248 2866
Fragments arranged by size:
2866 919 535 248 72
NcoI C'CATG_G
Cuts at: 1364 1364
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 2819 2819
Size: 4640
NspI r_CATG'y
Cuts at: 1188 1693 2193 3100 1188
Size: 505 500 907 2728
Fragments arranged by size:
2728 907 505 500
PasI CC'CwG_GG
Cuts at: 621 621
Size: 4640
PciI A'CATG_T
Cuts at: 3096 3096
Size: 4640
PflMI CCAn_nnn'nTGG
Cuts at: 347 675 710 942 1170 1316 347
Size: 328 35 232 228 146 3671
Fragments arranged by size:
3671 328 232 228 146 35
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PpuMI rG'GwC_Cy
Cuts at: 173 310 1348 173
Size: 137 1038 3465
Fragments arranged by size:
3465 1038 137
PshAI GACnn'nnGTC
Cuts at: 178 178
Size: 4640
PspOMI G'GGCC_C
Cuts at: 350 1444 350
Size: 1094 3546
PspXI vC'TCGA_Gb
Cuts at: 1520 1520
Size: 4640
PstI C_TGCA'G
Cuts at: 218 755 992 1687 2372 218
Size: 537 237 695 685 2486
Fragments arranged by size:
2486 695 685 537 237
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 756 993 1549 2065 2425 756
Size: 237 556 516 360 2971
Fragments arranged by size:
2971 556 516 360 237
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 1337 1654 1337
Size: 317 4323
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SanDI GG'GwC_CC
Cuts at: 173 310 1348 173
Size: 137 1038 3465
Fragments arranged by size:
3465 1038 137
SapI GCTCTTCn'nnn_
Cuts at: 1342 2663 2873 1342
Size: 1321 210 3109
Fragments arranged by size:
3109 1321 210
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SfcI C'TryA_G
Cuts at: 33 214 751 988 1683 2368 3361 3552 33
Size: 181 537 237 695 685 993 191 1121
Fragments arranged by size:
1121 993 695 685 537 237 191 181
SfoI GGC'GCC
Cuts at: 546 618 1153 2072 2320 546
Size: 72 535 919 248 2866
Fragments arranged by size:
2866 919 535 248 72
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 814 1503 1520 1606 1795 2710 3202 3464 814
Size: 689 17 86 189 915 492 262 1990
Fragments arranged by size:
1990 915 689 492 262 189 86 17
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StyI C'CwwG_G
Cuts at: 525 1118 1315 1364 1774 2128 525
Size: 593 197 49 410 354 3037
Fragments arranged by size:
3037 593 410 354 197 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2223 2356 2815 3024 2223
Size: 133 459 209 3839
Fragments arranged by size:
3839 459 209 133
TatI w'GTAC_w
Cuts at: 4155 4155
Size: 4640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1082 1114 4157 4189 1082
Size: 32 3043 32 1533
Fragments arranged by size:
3043 1533 32 32
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 1006 2135 372
Size: 634 1129 2877
Fragments arranged by size:
2877 1129 634
XhoI C'TCGA_G
Cuts at: 814 1520 814
Size: 706 3934
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BmtI BseRI BsgI BsiWI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PsiI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI