(Circular) (Six-base) MAPSORT of: pF1KB0464.seq Check: 4722 from: 1 to: 6071 pF1KB0464 6071 bp With 182 enzymes: SgfI * August 25, 2016 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1719 1719 Size: 6071 AccI GT'mk_AC Cuts at: 3109 3109 Size: 6071 Acc65I G'GTAC_C Cuts at: 3087 3087 Size: 6071 AclI AA'CG_TT Cuts at: 5911 5911 Size: 6071 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 319 1249 1738 2603 3896 4328 5075 5343 319 Size: 930 489 865 1293 432 747 268 1047 Fragments arranged by size: 1293 1047 930 865 747 489 432 268 AflII C'TTAA_G Cuts at: 1075 1075 Size: 6071 AflIII A'CryG_T Cuts at: 1577 3423 4527 5504 1577 Size: 1846 1104 977 2144 Fragments arranged by size: 2144 1846 1104 977 AgeI A'CCGG_T Cuts at: 1040 4459 1040 Size: 3419 2652 AhdI GACnn_n'nnGTC Cuts at: 2450 2450 Size: 6071 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1357 2899 1357 Size: 1542 4529 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1761 1793 1761 Size: 32 6039 AlwNI CAG_nnn'CTG Cuts at: 990 1510 3376 4943 990 Size: 520 1866 1567 2118 Fragments arranged by size: 2118 1866 1567 520 ApaI G_GGCC'C Cuts at: 2109 2109 Size: 6071 ApaLI G'TGCA_C Cuts at: 1581 2154 4841 1581 Size: 573 2687 2811 Fragments arranged by size: 2811 2687 573 ApoI r'AATT_y Cuts at: 247 291 1259 2685 2946 3033 3075 5424 5680 Size: 44 968 1426 261 87 42 2349 256 Cuts at: 5680 247 Size: 638 Fragments arranged by size: 2349 1426 968 638 261 256 87 44 42 AseI AT'TA_AT Cuts at: 20 20 Size: 6071 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6071 AvaI C'yCGr_G Cuts at: 3091 3091 Size: 6071 AvrII C'CTAG_G Cuts at: 1053 2700 1053 Size: 1647 4424 BamHI G'GATC_C Cuts at: 637 3096 637 Size: 2459 3612 BanI G'GyrC_C Cuts at: 1282 2263 3087 3501 3749 3784 1282 Size: 981 824 414 248 35 3569 Fragments arranged by size: 3569 981 824 414 248 35 BanII G_rGCy'C Cuts at: 414 1621 2109 2244 2698 3085 4115 414 Size: 1207 488 135 454 387 1030 2370 Fragments arranged by size: 2370 1207 1030 488 454 387 135 BbeI G_GCGC'C Cuts at: 3505 3753 3505 Size: 248 5823 BbsI GAAGACnn'nnnn_ Cuts at: 623 1403 2524 2801 623 Size: 780 1121 277 3893 Fragments arranged by size: 3893 1121 780 277 BbvCI CC'TCA_GC Cuts at: 1012 1741 2012 1012 Size: 729 271 5071 Fragments arranged by size: 5071 729 271 BciVI GTATCCnnnnn_n' Cuts at: 3969 4730 6071 3969 Size: 761 1341 3969 Fragments arranged by size: 3969 1341 761 BclI T'GATC_A Cuts at: 1688 3591 1688 Size: 1903 4168 BglII A'GATC_T Cuts at: 2557 2816 3586 5623 2557 Size: 259 770 2037 3005 Fragments arranged by size: 3005 2037 770 259 BlpI GC'TnA_GC Cuts at: 1070 3183 1070 Size: 2113 3958 Bme1580I G_kGCm'C Cuts at: 1431 1585 2109 2123 2158 3696 3789 4845 1431 Size: 154 524 14 35 1538 93 1056 2657 Fragments arranged by size: 2657 1538 1056 524 154 93 35 14 BmgBI CAC'GTC Cuts at: 2478 2478 Size: 6071 BmrI ACTGGGnnnn_n' Cuts at: 1333 1564 1995 3283 3364 3699 5843 1333 Size: 231 431 1288 81 335 2144 1561 Fragments arranged by size: 2144 1561 1288 431 335 231 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 433 1851 2541 4438 433 Size: 1418 690 1897 2066 Fragments arranged by size: 2066 1897 1418 690 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 484 961 1452 1696 1821 1909 2258 484 Size: 477 491 244 125 88 349 4297 Fragments arranged by size: 4297 491 477 349 244 125 88 Bpu10I CC'TnA_GC Cuts at: 1012 1741 2012 1012 Size: 729 271 5071 Fragments arranged by size: 5071 729 271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3247 4126 4618 4916 3247 Size: 879 492 298 4402 Fragments arranged by size: 4402 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1239 2133 5775 1239 Size: 894 3642 1535 Fragments arranged by size: 3642 1535 894 BsaAI yAC'GTr Cuts at: 1578 3424 1578 Size: 1846 4225 BsaBI GATnn'nnATC Cuts at: 6 2186 2666 3585 5622 6 Size: 2180 480 919 2037 455 Fragments arranged by size: 2180 2037 919 480 455 BsaHI Gr'CG_yC Cuts at: 3502 3750 4452 5948 3502 Size: 248 702 1496 3625 Fragments arranged by size: 3625 1496 702 248 BsaWI w'CCGG_w Cuts at: 597 1040 3260 3781 4459 4733 4880 5291 597 Size: 443 2220 521 678 274 147 411 1377 Fragments arranged by size: 2220 1377 678 521 443 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2113 2143 2394 2424 2889 2919 2113 Size: 30 251 30 465 30 5265 Fragments arranged by size: 5265 465 251 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1482 1750 2121 1482 Size: 268 371 5432 Fragments arranged by size: 5432 371 268 BseYI C'CCAG_C Cuts at: 748 790 1485 1510 2025 2986 3496 4254 4831 Size: 42 695 25 515 961 510 758 577 Cuts at: 4831 5327 748 Size: 496 1492 Fragments arranged by size: 1492 961 758 695 577 515 510 496 42 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1543 2144 1543 Size: 601 5470 BsiEI CG_ry'CG Cuts at: 104 3613 3659 4867 104 Size: 3509 46 1208 1308 Fragments arranged by size: 3509 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1585 2158 2178 3085 3863 4053 4845 1585 Size: 573 20 907 778 190 792 2811 Fragments arranged by size: 2811 907 792 778 573 190 20 BsmI GAATG_Cn' Cuts at: 129 2016 129 Size: 1887 4184 Bsp1286I G_dGCh'C Cuts at: 414 1431 1585 1621 2109 2123 2158 2178 2244 Size: 1017 154 36 488 14 35 20 66 Cuts at: 2244 2698 3085 3696 3789 3863 4053 4115 4845 Size: 454 387 611 93 74 190 62 730 Cuts at: 4845 414 Size: 1640 Fragments arranged by size: 1640 1017 730 611 488 454 387 190 154 93 74 66 62 36 35 20 14 BspEI T'CCGG_A Cuts at: 597 5291 597 Size: 4694 1377 BspHI T'CATG_A Cuts at: 1598 5247 1598 Size: 3649 2422 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1719 3121 3335 3637 4018 1719 Size: 1402 214 302 381 3772 Fragments arranged by size: 3772 1402 381 302 214 BsrBI CCG'CTC Cuts at: 50 4363 4417 5901 6066 50 Size: 4313 54 1484 165 55 Fragments arranged by size: 4313 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2883 3983 2883 Size: 1100 4971 BsrFI r'CCGG_y Cuts at: 1040 1304 1902 4069 4250 4459 1040 Size: 264 598 2167 181 209 2652 Fragments arranged by size: 2652 2167 598 264 209 181 BsrGI T'GTAC_A Cuts at: 2298 2298 Size: 6071 BssSI C'ACGA_G Cuts at: 4342 4700 4342 Size: 358 5713 BstAPI GCAn_nnn'nTGC Cuts at: 135 3448 135 Size: 3313 2758 BstBI TT'CG_AA Cuts at: 1257 4432 1257 Size: 3175 2896 BstEII G'GTnAC_C Cuts at: 523 523 Size: 6071 BstXI CCAn_nnnn'nTGG Cuts at: 834 1933 834 Size: 1099 4972 BstYI r'GATC_y Cuts at: 1 637 699 2557 2630 2816 3096 3563 3586 Size: 636 62 1858 73 186 280 467 23 Cuts at: 3586 3921 4167 5179 5265 5277 5623 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1858 1012 636 467 449 346 335 280 246 186 86 73 62 23 12 BtgI C'CryG_G Cuts at: 548 2918 3041 548 Size: 2370 123 3578 Fragments arranged by size: 3578 2370 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 599 4021 4202 4365 599 Size: 3422 181 163 2305 Fragments arranged by size: 3422 2305 181 163 BtsI GCAGTG_nn' Cuts at: 365 2156 3067 3314 365 Size: 1791 911 247 3122 Fragments arranged by size: 3122 1791 911 247 ClaI AT'CG_AT Cuts at: 3002 3002 Size: 6071 DraI TTT'AAA Cuts at: 3070 3070 Size: 6071 DrdI GACnn_nn'nnGTC Cuts at: 1504 3777 4635 1504 Size: 2273 858 2940 Fragments arranged by size: 2940 2273 858 EaeI y'GGCC_r Cuts at: 2184 3656 3830 4221 4248 5593 2184 Size: 1472 174 391 27 1345 2662 Fragments arranged by size: 2662 1472 1345 391 174 27 EagI C'GGCC_G Cuts at: 3656 3656 Size: 6071 EarI CTCTTCn'nnn_ Cuts at: 563 1119 1191 1422 1820 2331 2622 2738 2806 Size: 556 72 231 398 511 291 116 68 Cuts at: 2806 2868 4094 4304 563 Size: 62 1226 210 2330 Fragments arranged by size: 2330 1226 556 511 398 291 231 210 116 72 68 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2930 4279 4589 4735 5881 2930 Size: 1349 310 146 1146 3120 Fragments arranged by size: 3120 1349 1146 310 146 EcoICRI GAG'CTC Cuts at: 3083 3083 Size: 6071 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 319 484 961 1249 1452 1696 1738 1821 1909 Size: 165 477 288 203 244 42 83 88 Cuts at: 1909 2258 2603 3896 4328 5075 5343 319 Size: 349 345 1293 432 747 268 1047 Fragments arranged by size: 1293 1047 747 477 432 349 345 288 268 244 203 165 88 83 42 EcoNI CCTnn'n_nnAGG Cuts at: 674 1277 674 Size: 603 5468 EcoO109I rG'GnC_Cy Cuts at: 191 870 1514 2105 2827 3210 191 Size: 679 644 591 722 383 3052 Fragments arranged by size: 3052 722 679 644 591 383 EcoRI G'AATT_C Cuts at: 247 1259 3033 3075 247 Size: 1012 1774 42 3243 Fragments arranged by size: 3243 1774 1012 42 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1090 4090 1090 Size: 3000 3071 FspI TGC'GCA Cuts at: 3852 3852 Size: 6071 HaeII r_GCGC'y Cuts at: 2969 3505 3753 4775 5754 2969 Size: 536 248 1022 979 3286 Fragments arranged by size: 3286 1022 979 536 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 666 698 724 756 2436 2468 2761 2793 3913 Size: 32 26 32 1680 32 293 32 1120 Cuts at: 3913 3943 3945 3975 666 Size: 30 2 30 2762 Fragments arranged by size: 2762 1680 1120 293 32 32 32 32 30 30 26 2 HincII GTy'rAC Cuts at: 3110 3110 Size: 6071 HindIII A'AGCT_T Cuts at: 359 892 2196 2892 359 Size: 533 1304 696 3538 Fragments arranged by size: 3538 1304 696 533 KasI G'GCGC_C Cuts at: 3501 3749 3501 Size: 248 5823 KpnI G_GTAC'C Cuts at: 3091 3091 Size: 6071 MluI A'CGCG_T Cuts at: 5504 5504 Size: 6071 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 409 1460 2596 4742 4926 409 Size: 1051 1136 2146 184 1554 Fragments arranged by size: 2146 1554 1136 1051 184 MscI TGG'CCA Cuts at: 2186 3832 2186 Size: 1646 4425 MslI CAynn'nnrTG Cuts at: 805 2756 4060 4187 805 Size: 1951 1304 127 2689 Fragments arranged by size: 2689 1951 1304 127 MspA1I CmG'CkG Cuts at: 244 634 990 1794 1841 2025 3182 3496 3856 Size: 390 356 804 47 184 1157 314 360 Cuts at: 3856 4869 5114 244 Size: 1013 245 1201 Fragments arranged by size: 1201 1157 1013 804 390 360 356 314 245 184 47 NaeI GCC'GGC Cuts at: 4252 4252 Size: 6071 NarI GG'CG_CC Cuts at: 3502 3750 3502 Size: 248 5823 NcoI C'CATG_G Cuts at: 2918 3041 2918 Size: 123 5948 NgoMIV G'CCGG_C Cuts at: 4250 4250 Size: 6071 NspI r_CATG'y Cuts at: 814 3124 3624 4531 814 Size: 2310 500 907 2354 Fragments arranged by size: 2354 2310 907 500 PasI CC'CwG_GG Cuts at: 2109 2909 2109 Size: 800 5271 PciI A'CATG_T Cuts at: 4527 4527 Size: 6071 PflMI CCAn_nnn'nTGG Cuts at: 2924 2924 Size: 6071 PfoI T'CCnGG_A Cuts at: 1319 2471 3008 1319 Size: 1152 537 4382 Fragments arranged by size: 4382 1152 537 PmeI GTTT'AAAC Cuts at: 3070 3070 Size: 6071 PmlI CAC'GTG Cuts at: 1578 1578 Size: 6071 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5210 5242 5210 Size: 32 6039 PpuMI rG'GwC_Cy Cuts at: 191 870 1514 191 Size: 679 644 4748 Fragments arranged by size: 4748 679 644 PshAI GACnn'nnGTC Cuts at: 2255 2255 Size: 6071 PspOMI G'GGCC_C Cuts at: 2105 2105 Size: 6071 PstI C_TGCA'G Cuts at: 372 1001 2793 2888 3118 3803 372 Size: 629 1792 95 230 685 2640 Fragments arranged by size: 2640 1792 685 629 230 95 PvuI CG_AT'CG Cuts at: 104 104 Size: 6071 PvuII CAG'CTG Cuts at: 244 990 1841 3496 3856 244 Size: 746 851 1655 360 2459 Fragments arranged by size: 2459 1655 851 746 360 RsrII CG'GwC_CG Cuts at: 4266 4266 Size: 6071 SacI G_AGCT'C Cuts at: 3085 3085 Size: 6071 SalI G'TCGA_C Cuts at: 3108 3108 Size: 6071 SanDI GG'GwC_CC Cuts at: 1514 1514 Size: 6071 SapI GCTCTTCn'nnn_ Cuts at: 2738 4094 4304 2738 Size: 1356 210 4505 Fragments arranged by size: 4505 1356 210 SbfI CC_TGCA'GG Cuts at: 3118 3118 Size: 6071 ScaI AGT'ACT Cuts at: 5588 5588 Size: 6071 SexAI A'CCwGG_T Cuts at: 527 527 Size: 6071 SfcI C'TryA_G Cuts at: 33 368 997 2789 2884 3114 3799 4792 4983 Size: 335 629 1792 95 230 685 993 191 Cuts at: 4983 33 Size: 1121 Fragments arranged by size: 1792 1121 993 685 629 335 230 191 95 SfoI GGC'GCC Cuts at: 3503 3751 3503 Size: 248 5823 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6071 SmaI CCC'GGG Cuts at: 3093 3093 Size: 6071 SmlI C'TyrA_G Cuts at: 1075 3226 4141 4633 4895 1075 Size: 2151 915 492 262 2251 Fragments arranged by size: 2251 2151 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6071 SphI G_CATG'C Cuts at: 814 3124 3624 814 Size: 2310 500 3261 Fragments arranged by size: 3261 2310 500 StyI C'CwwG_G Cuts at: 213 833 866 1053 2700 2918 3041 3205 3559 Size: 620 33 187 1647 218 123 164 354 Cuts at: 3559 213 Size: 2725 Fragments arranged by size: 2725 1647 620 354 218 187 164 123 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 826 1401 1913 2085 3654 3787 4246 4455 826 Size: 575 512 172 1569 133 459 209 2442 Fragments arranged by size: 2442 1569 575 512 459 209 172 133 TatI w'GTAC_w Cuts at: 908 1974 2298 5586 908 Size: 1066 324 3288 1393 Fragments arranged by size: 3288 1393 1066 324 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2113 2145 2889 2921 5588 5620 2113 Size: 32 744 32 2667 32 2564 Fragments arranged by size: 2667 2564 744 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3868 3868 Size: 6071 XbaI T'CTAG_A Cuts at: 3102 3102 Size: 6071 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1327 2925 3566 114 Size: 1213 1598 641 2619 Fragments arranged by size: 2619 1598 1213 641 XmaI C'CCGG_G Cuts at: 3091 3091 Size: 6071 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AleI AscI BaeI BcgI BfrBI BglI BmtI BsiWI BsmBI BssHII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI