Restriction map for
pF1KB0467
(Circular) (Six-base) MAPSORT of: pF1KB0467.seq Check: 1530 from: 1 to: 4655
pF1KB0467 4655 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1522 1522
Size: 4655
AccI GT'mk_AC
Cuts at: 1693 1693
Size: 4655
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 1431 4495 1431
Size: 3064 1591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1444 2480 2912 3659 3927 880
Size: 564 1036 432 747 268 1608
Fragments arranged by size:
1608 1036 747 564 432 268
AflIII A'CryG_T
Cuts at: 2007 3111 4088 2007
Size: 1104 977 2574
Fragments arranged by size:
2574 1104 977
AgeI A'CCGG_T
Cuts at: 1647 3043 1647
Size: 1396 3259
AlwNI CAG_nnn'CTG
Cuts at: 858 1960 3527 858
Size: 1102 1567 1986
Fragments arranged by size:
1986 1567 1102
ApaI G_GGCC'C
Cuts at: 154 1059 154
Size: 905 3750
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 1183 1413 1659 4008 4264 1183
Size: 230 246 2349 256 1574
Fragments arranged by size:
2349 1574 256 246 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 127 1671 2085 2333 2368 127
Size: 1544 414 248 35 2414
Fragments arranged by size:
2414 1544 414 248 35
BanII G_rGCy'C
Cuts at: 154 599 1059 1669 2699 154
Size: 445 460 610 1030 2110
Fragments arranged by size:
2110 1030 610 460 445
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 734 1211 734
Size: 477 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 837 871 837
Size: 34 4621
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3314 4655 2553
Size: 761 1341 2553
Fragments arranged by size:
2553 1341 761
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BglI GCCn_nnn'nGGC
Cuts at: 763 763
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 206 676 1767 206
Size: 470 1091 3094
Fragments arranged by size:
3094 1091 470
Bme1580I G_kGCm'C
Cuts at: 154 1059 2280 2373 3429 154
Size: 905 1221 93 1056 1380
Fragments arranged by size:
1380 1221 1056 905 93
BmgBI CAC'GTC
Cuts at: 1156 1156
Size: 4655
BmrI ACTGGGnnnn_n'
Cuts at: 1628 1867 1948 2283 4427 1628
Size: 239 81 335 2144 1856
Fragments arranged by size:
2144 1856 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
Bpu10I CC'TnA_GC
Cuts at: 1172 1172
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 412 2008 412
Size: 1596 3059
BsaBI GATnn'nnATC
Cuts at: 6 1468 2169 4206 6
Size: 1462 701 2037 455
Fragments arranged by size:
2037 1462 701 455
BsaHI Gr'CG_yC
Cuts at: 1425 2086 2334 3036 4532 1425
Size: 661 248 702 1496 1548
Fragments arranged by size:
1548 1496 702 661 248
BsaWI w'CCGG_w
Cuts at: 1647 1844 2365 3043 3317 3464 3875 1647
Size: 197 521 678 274 147 411 2427
Fragments arranged by size:
2427 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1564 1594 1564
Size: 30 4625
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 607 655 685 783 883 493
Size: 114 48 30 98 100 4265
Fragments arranged by size:
4265 114 100 98 48 30
BseYI C'CCAG_C
Cuts at: 2080 2838 3415 3911 2080
Size: 758 577 496 2824
Fragments arranged by size:
2824 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 599 1669 2447 2637 3429 212
Size: 387 1070 778 190 792 1438
Fragments arranged by size:
1438 1070 792 778 387 190
BsmI GAATG_Cn'
Cuts at: 816 816
Size: 4655
BsmBI CGTCTCn'nnnn_
Cuts at: 1013 1146 1013
Size: 133 4522
Bsp1286I G_dGCh'C
Cuts at: 154 212 599 1059 1669 2280 2373 2447 2637
Size: 58 387 460 610 611 93 74 190
Cuts at: 2637 2699 3429 154
Size: 62 730 1380
Fragments arranged by size:
1380 730 611 610 460 387 190 93
74 62 58
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 3831 3831
Size: 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1522 1705 1919 2221 2602 1522
Size: 183 214 302 381 3575
Fragments arranged by size:
3575 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 282 645 2947 3001 4485 4650 50
Size: 232 363 2302 54 1484 165 55
Fragments arranged by size:
2302 1484 363 232 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 2567
Size: 4655
BsrFI r'CCGG_y
Cuts at: 1647 2653 2834 3043 1647
Size: 1006 181 209 3259
Fragments arranged by size:
3259 1006 209 181
BsrGI T'GTAC_A
Cuts at: 110 1291 110
Size: 1181 3474
BssSI C'ACGA_G
Cuts at: 1045 2926 3284 1045
Size: 1881 358 2416
Fragments arranged by size:
2416 1881 358
BstAPI GCAn_nnn'nTGC
Cuts at: 658 1628 2032 658
Size: 970 404 3281
Fragments arranged by size:
3281 970 404
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 124 124
Size: 4655
BstYI r'GATC_y
Cuts at: 1 178 1680 2147 2170 2505 2751 3763 3849
Size: 177 1502 467 23 335 246 1012 86
Cuts at: 3849 3861 4207 1
Size: 12 346 449
Fragments arranged by size:
1502 1012 467 449 346 335 246 177
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 1898 1898
Size: 4655
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 2361 3219 2361
Size: 858 3797
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 692 860 1070 1397 1502 2678 2888 692
Size: 168 210 327 105 1176 210 2459
Fragments arranged by size:
2459 1176 327 210 210 168 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 597 1667 597
Size: 1070 3585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1444 2480 2912 3659 3927 880
Size: 564 1036 432 747 268 1608
Fragments arranged by size:
1608 1036 747 564 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 191 191
Size: 4655
EcoO109I rG'GnC_Cy
Cuts at: 150 151 1055 1056 1575 1794 150
Size: 1 904 1 519 219 3011
Fragments arranged by size:
3011 904 519 219 1 1
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4655
FspI TGC'GCA
Cuts at: 1621 2436 1621
Size: 815 3840
HaeII r_GCGC'y
Cuts at: 2089 2337 3359 4338 2089
Size: 248 1022 979 2406
Fragments arranged by size:
2406 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 338 370 1564 1596 2497 2527 2529 2559 338
Size: 32 1194 32 901 30 2 30 2434
Fragments arranged by size:
2434 1194 901 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
HindIII A'AGCT_T
Cuts at: 1639 1639
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MfeI C'AATT_G
Cuts at: 457 457
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 252 3326 3510 252
Size: 3074 184 1397
Fragments arranged by size:
3074 1397 184
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 914 1373 2644 2771 914
Size: 459 1271 127 2798
Fragments arranged by size:
2798 1271 459 127
MspA1I CmG'CkG
Cuts at: 120 138 855 1766 2080 2440 3453 3698 120
Size: 18 717 911 314 360 1013 245 1077
Fragments arranged by size:
1077 1013 911 717 360 314 245 18
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NspI r_CATG'y
Cuts at: 1631 1708 2208 3115 1631
Size: 77 500 907 3171
Fragments arranged by size:
3171 907 500 77
PciI A'CATG_T
Cuts at: 3111 3111
Size: 4655
PflMI CCAn_nnn'nTGG
Cuts at: 719 719
Size: 4655
PfoI T'CCnGG_A
Cuts at: 1568 1568
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 691 723 3794 3826 691
Size: 32 3071 32 1520
Fragments arranged by size:
3071 1520 32 32
PpuMI rG'GwC_Cy
Cuts at: 1575 1575
Size: 4655
PspOMI G'GGCC_C
Cuts at: 150 1055 150
Size: 905 3750
PstI C_TGCA'G
Cuts at: 1702 2387 1702
Size: 685 3970
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 855 2080 2440 855
Size: 1225 360 3070
Fragments arranged by size:
3070 1225 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 599 1669 599
Size: 1070 3585
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 692 2678 2888 692
Size: 1986 210 2459
Fragments arranged by size:
2459 1986 210
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SfcI C'TryA_G
Cuts at: 33 1698 2383 3376 3567 33
Size: 1665 685 993 191 1121
Fragments arranged by size:
1665 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1631 1708 2208 1631
Size: 77 500 4078
Fragments arranged by size:
4078 500 77
SspI AAT'ATT
Cuts at: 1141 1196 1141
Size: 55 4600
StyI C'CwwG_G
Cuts at: 198 740 1789 2143 198
Size: 542 1049 354 2710
Fragments arranged by size:
2710 1049 542 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 746 1600 2238 2371 2830 3039 746
Size: 854 638 133 459 209 2362
Fragments arranged by size:
2362 854 638 459 209 133
TatI w'GTAC_w
Cuts at: 110 1291 1445 4170 110
Size: 1181 154 2725 595
Fragments arranged by size:
2725 1181 595 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 4623
Tth111I GACn'n_nGTC
Cuts at: 1564 2452 1564
Size: 888 3767
XbaI T'CTAG_A
Cuts at: 216 1686 216
Size: 1470 3185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 162 2150 162
Size: 1988 2667
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
XmnI GAAnn'nnTTC
Cuts at: 1001 1001
Size: 4655
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BpmI BsgI BsiWI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI