Restriction map for
pF1KB0473
(Circular) (Six-base) MAPSORT of: pF1KB0473.seq Check: 1647 from: 1 to: 4610
pF1KB0473 4610 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 894 894
Size: 4610
AccI GT'mk_AC
Cuts at: 320 1309 1648 320
Size: 989 339 3282
Fragments arranged by size:
3282 989 339
Acc65I G'GTAC_C
Cuts at: 964 1626 964
Size: 662 3948
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1580 2435 2867 3614 3882 163
Size: 1417 855 432 747 268 891
Fragments arranged by size:
1417 891 855 747 432 268
AflIII A'CryG_T
Cuts at: 404 1962 3066 4043 404
Size: 1558 1104 977 971
Fragments arranged by size:
1558 1104 977 971
AgeI A'CCGG_T
Cuts at: 2998 2998
Size: 4610
AleI CACnn'nnGTG
Cuts at: 1072 1072
Size: 4610
AlwNI CAG_nnn'CTG
Cuts at: 907 916 1017 1290 1915 3482 907
Size: 9 101 273 625 1567 2035
Fragments arranged by size:
2035 1567 625 273 101 9
ApaLI G'TGCA_C
Cuts at: 625 3380 625
Size: 2755 1855
ApoI r'AATT_y
Cuts at: 1614 3963 4219 1614
Size: 2349 256 2005
Fragments arranged by size:
2349 2005 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 160 701 1204 1254 1630 160
Size: 541 503 50 376 3140
Fragments arranged by size:
3140 541 503 376 50
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 964 1053 1626 2040 2288 2323 964
Size: 89 573 414 248 35 3251
Fragments arranged by size:
3251 573 414 248 89 35
BanII G_rGCy'C
Cuts at: 331 1172 1624 2654 331
Size: 841 452 1030 2287
Fragments arranged by size:
2287 1030 841 452
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbsI GAAGACnn'nnnn_
Cuts at: 773 773
Size: 4610
BbvCI CC'TCA_GC
Cuts at: 584 584
Size: 4610
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3269 4610 2508
Size: 761 1341 2508
Fragments arranged by size:
2508 1341 761
BclI T'GATC_A
Cuts at: 2130 2130
Size: 4610
BglI GCCn_nnn'nGGC
Cuts at: 1516 1516
Size: 4610
BglII A'GATC_T
Cuts at: 1469 2125 4162 1469
Size: 656 2037 1917
Fragments arranged by size:
2037 1917 656
BlpI GC'TnA_GC
Cuts at: 284 1228 1722 284
Size: 944 494 3172
Fragments arranged by size:
3172 944 494
Bme1580I G_kGCm'C
Cuts at: 629 976 1024 2235 2328 3384 629
Size: 347 48 1211 93 1056 1855
Fragments arranged by size:
1855 1211 1056 347 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 753 1822 1903 2238 4382 753
Size: 1069 81 335 2144 981
Fragments arranged by size:
2144 1069 981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 1079 1186 893
Size: 186 107 4317
Fragments arranged by size:
4317 186 107
Bpu10I CC'TnA_GC
Cuts at: 584 932 584
Size: 348 4262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1786 2665 3157 3455 1786
Size: 879 492 298 2941
Fragments arranged by size:
2941 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1165 4314 1165
Size: 3149 1461
BsaAI yAC'GTr
Cuts at: 324 615 1963 324
Size: 291 1348 2971
Fragments arranged by size:
2971 1348 291
BsaBI GATnn'nnATC
Cuts at: 6 2124 4161 6
Size: 2118 2037 455
Fragments arranged by size:
2118 2037 455
BsaHI Gr'CG_yC
Cuts at: 157 2041 2289 2991 4487 157
Size: 1884 248 702 1496 280
Fragments arranged by size:
1884 1496 702 280 248
BsaWI w'CCGG_w
Cuts at: 1799 2320 2998 3272 3419 3830 1799
Size: 521 678 274 147 411 2579
Fragments arranged by size:
2579 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 941 971 941
Size: 30 4580
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 1207 1396 1456 1459 601
Size: 606 189 60 3 3752
Fragments arranged by size:
3752 606 189 60 3
BseYI C'CCAG_C
Cuts at: 138 345 510 541 560 960 993 1010 1080
Size: 207 165 31 19 400 33 17 70
Cuts at: 1080 1155 2035 2793 3370 3866 138
Size: 75 880 758 577 496 882
Fragments arranged by size:
882 880 758 577 496 400 207 165
75 70 33 31 19 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 675 458
Size: 217 4393
BsiEI CG_ry'CG
Cuts at: 104 2152 2198 3406 104
Size: 2048 46 1208 1308
Fragments arranged by size:
2048 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 127 629 1244 1624 2402 2592 3384 127
Size: 502 615 380 778 190 792 1353
Fragments arranged by size:
1353 792 778 615 502 380 190
Bsp1286I G_dGCh'C
Cuts at: 127 331 629 976 1024 1172 1244 1624 2235
Size: 204 298 347 48 148 72 380 611
Cuts at: 2235 2328 2402 2592 2654 3384 127
Size: 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 380 347 298 204 190
148 93 74 72 62 48
BspEI T'CCGG_A
Cuts at: 3830 3830
Size: 4610
BspHI T'CATG_A
Cuts at: 3786 3786
Size: 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 894 1660 1874 2176 2557 278
Size: 616 766 214 302 381 2331
Fragments arranged by size:
2331 766 616 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1215 2902 2956 4440 4605 50
Size: 1165 1687 54 1484 165 55
Fragments arranged by size:
1687 1484 1165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2522 2522
Size: 4610
BsrFI r'CCGG_y
Cuts at: 2608 2789 2998 2608
Size: 181 209 4220
Fragments arranged by size:
4220 209 181
BssSI C'ACGA_G
Cuts at: 2881 3239 2881
Size: 358 4252
BstAPI GCAn_nnn'nTGC
Cuts at: 907 916 1290 1987 907
Size: 9 374 697 3530
Fragments arranged by size:
3530 697 374 9
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstEII G'GTnAC_C
Cuts at: 1101 1101
Size: 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 1057 1057
Size: 4610
BstYI r'GATC_y
Cuts at: 1 1469 1635 2102 2125 2460 2706 3718 3804
Size: 1468 166 467 23 335 246 1012 86
Cuts at: 3804 3816 4162 1
Size: 12 346 449
Fragments arranged by size:
1468 1012 467 449 346 335 246 166
86 23 12
BtgI C'CryG_G
Cuts at: 107 200 107
Size: 93 4517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 225 2560 2741 2904 225
Size: 2335 181 163 1931
Fragments arranged by size:
2335 1931 181 163
BtsI GCAGTG_nn'
Cuts at: 914 947 1853 914
Size: 33 906 3671
Fragments arranged by size:
3671 906 33
ClaI AT'CG_AT
Cuts at: 1352 1352
Size: 4610
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DraIII CAC_nnn'GTG
Cuts at: 1278 1278
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3174 2316
Size: 858 3752
EaeI y'GGCC_r
Cuts at: 302 807 944 1139 1216 1527 1542 2195 2369
Size: 505 137 195 77 311 15 653 174
Cuts at: 2369 2760 2787 4132 302
Size: 391 27 1345 780
Fragments arranged by size:
1345 780 653 505 391 311 195 174
137 77 27 15
EagI C'GGCC_G
Cuts at: 2195 2195
Size: 4610
EarI CTCTTCn'nnn_
Cuts at: 1184 2633 2843 1184
Size: 1449 210 2951
Fragments arranged by size:
2951 1449 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 2818 3128 3274 4420 352
Size: 2466 310 146 1146 542
Fragments arranged by size:
2466 1146 542 310 146
EcoICRI GAG'CTC
Cuts at: 1622 1622
Size: 4610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 893 1079 1186 1580 2435 2867 3614 3882
Size: 730 186 107 394 855 432 747 268
Cuts at: 3882 163
Size: 891
Fragments arranged by size:
891 855 747 730 432 394 268 186
107
EcoNI CCTnn'n_nnAGG
Cuts at: 181 181
Size: 4610
EcoO109I rG'GnC_Cy
Cuts at: 174 462 633 705 1518 1749 174
Size: 288 171 72 813 231 3035
Fragments arranged by size:
3035 813 288 231 171 72
EcoRI G'AATT_C
Cuts at: 1614 1614
Size: 4610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 4610
FspI TGC'GCA
Cuts at: 1327 2391 1327
Size: 1064 3546
HaeII r_GCGC'y
Cuts at: 301 1268 2044 2292 3314 4293 301
Size: 967 776 248 1022 979 618
Fragments arranged by size:
1022 979 967 776 618 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 763 795 915 947 2452 2482 2484
Size: 32 464 32 120 32 1505 30 2
Cuts at: 2484 2514 267
Size: 30 2363
Fragments arranged by size:
2363 1505 464 120 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1649 1649
Size: 4610
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 968 1630 968
Size: 662 3948
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1174 3281 3465 1174
Size: 2107 184 2319
Fragments arranged by size:
2319 2107 184
MscI TGG'CCA
Cuts at: 304 809 1141 2371 304
Size: 505 332 1230 2543
Fragments arranged by size:
2543 1230 505 332
MslI CAynn'nnrTG
Cuts at: 403 529 613 1072 1279 2599 2726 403
Size: 126 84 459 207 1320 127 2287
Fragments arranged by size:
2287 1320 459 207 127 126 84
MspA1I CmG'CkG
Cuts at: 288 294 480 892 904 955 1532 1721 2035
Size: 6 186 412 12 51 577 189 314
Cuts at: 2035 2395 3408 3653 288
Size: 360 1013 245 1245
Fragments arranged by size:
1245 1013 577 412 360 314 245 189
186 51 12 6
NaeI GCC'GGC
Cuts at: 2791 2791
Size: 4610
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NcoI C'CATG_G
Cuts at: 107 200 107
Size: 93 4517
NgoMIV G'CCGG_C
Cuts at: 2789 2789
Size: 4610
NspI r_CATG'y
Cuts at: 408 1663 2163 3070 408
Size: 1255 500 907 1948
Fragments arranged by size:
1948 1255 907 500
PasI CC'CwG_GG
Cuts at: 969 1017 969
Size: 48 4562
PciI A'CATG_T
Cuts at: 404 3066 404
Size: 2662 1948
PflMI CCAn_nnn'nTGG
Cuts at: 1017 1017
Size: 4610
PfoI T'CCnGG_A
Cuts at: 1163 1163
Size: 4610
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4578
PpuMI rG'GwC_Cy
Cuts at: 462 633 705 462
Size: 171 72 4367
Fragments arranged by size:
4367 171 72
PshAI GACnn'nnGTC
Cuts at: 987 987
Size: 4610
PstI C_TGCA'G
Cuts at: 275 912 1657 2342 275
Size: 637 745 685 2543
Fragments arranged by size:
2543 745 685 637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4610
PvuII CAG'CTG
Cuts at: 480 904 2035 2395 480
Size: 424 1131 360 2695
Fragments arranged by size:
2695 1131 424 360
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 1624 1624
Size: 4610
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SanDI GG'GwC_CC
Cuts at: 705 705
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 2633 2843 2633
Size: 210 4400
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 814 4127 814
Size: 3313 1297
SfcI C'TryA_G
Cuts at: 33 271 908 1310 1653 2338 3331 3522 33
Size: 238 637 402 343 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 402 343 238 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1516 1516
Size: 4610
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 703 1632 703
Size: 929 3681
SmlI C'TyrA_G
Cuts at: 1765 2680 3172 3434 1765
Size: 915 492 262 2941
Fragments arranged by size:
2941 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1663 2163 1663
Size: 500 4110
StuI AGG'CCT
Cuts at: 370 670 878 370
Size: 300 208 4102
Fragments arranged by size:
4102 300 208
StyI C'CwwG_G
Cuts at: 107 200 485 1343 1744 2098 107
Size: 93 285 858 401 354 2619
Fragments arranged by size:
2619 858 401 354 285 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 793 2193 2326 2785 2994 793
Size: 1400 133 459 209 2409
Fragments arranged by size:
2409 1400 459 209 133
TatI w'GTAC_w
Cuts at: 812 4125 812
Size: 3313 1297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 941 973 4127 4159 941
Size: 32 3154 32 1392
Fragments arranged by size:
3154 1392 32 32
Tth111I GACn'n_nGTC
Cuts at: 418 2407 418
Size: 1989 2621
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 1113 1522 2105 273
Size: 840 409 583 2778
Fragments arranged by size:
2778 840 583 409
XmaI C'CCGG_G
Cuts at: 701 1630 701
Size: 929 3681
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspOMI PspXI PsrI SacII
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI