Restriction map for
pF1KB0475
(Circular) (Six-base) MAPSORT of: pF1KB0475.seq Check: 5422 from: 1 to: 4664
pF1KB0475 4664 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 628 919 628
Size: 291 4373
AccI GT'mk_AC
Cuts at: 470 1702 470
Size: 1232 3432
Acc65I G'GTAC_C
Cuts at: 562 881 1680 562
Size: 319 799 3546
Fragments arranged by size:
3546 799 319
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 2921 3668 3936 2489
Size: 432 747 268 3217
Fragments arranged by size:
3217 747 432 268
AflIII A'CryG_T
Cuts at: 1289 2016 3120 4097 1289
Size: 727 1104 977 1856
Fragments arranged by size:
1856 1104 977 727
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AleI CACnn'nnGTG
Cuts at: 244 244
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 940 1353 1969 3536 940
Size: 413 616 1567 2068
Fragments arranged by size:
2068 1567 616 413
ApaLI G'TGCA_C
Cuts at: 3434 3434
Size: 4664
ApoI r'AATT_y
Cuts at: 400 1111 1668 4017 4273 400
Size: 711 557 2349 256 791
Fragments arranged by size:
2349 791 711 557 256
AscI GG'CGCG_CC
Cuts at: 1149 1149
Size: 4664
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 1684 1684
Size: 4664
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 130 562 747 881 1161 1247 1680 2094 2342
Size: 432 185 134 280 86 433 414 248
Cuts at: 2342 2377 130
Size: 35 2417
Fragments arranged by size:
2417 433 432 414 280 248 185 134
86 35
BanII G_rGCy'C
Cuts at: 1678 2708 1678
Size: 1030 3634
BbeI G_GCGC'C
Cuts at: 134 1251 2098 2346 134
Size: 1117 847 248 2452
Fragments arranged by size:
2452 1117 847 248
BbvCI CC'TCA_GC
Cuts at: 627 627
Size: 4664
BciVI GTATCCnnnnn_n'
Cuts at: 666 2562 3323 4664 666
Size: 1896 761 1341 666
Fragments arranged by size:
1896 1341 761 666
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 1776 1776
Size: 4664
Bme1580I G_kGCm'C
Cuts at: 752 1166 2289 2382 3438 752
Size: 414 1123 93 1056 1978
Fragments arranged by size:
1978 1123 1056 414 93
BmgBI CAC'GTC
Cuts at: 246 900 246
Size: 654 4010
BmrI ACTGGGnnnn_n'
Cuts at: 641 1408 1876 1957 2292 4436 641
Size: 767 468 81 335 2144 869
Fragments arranged by size:
2144 869 767 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 475 469
Size: 6 4658
Bpu10I CC'TnA_GC
Cuts at: 627 1083 1583 627
Size: 456 500 3708
Fragments arranged by size:
3708 500 456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1840 2719 3211 3509 1840
Size: 879 492 298 2995
Fragments arranged by size:
2995 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4368 4368
Size: 4664
BsaAI yAC'GTr
Cuts at: 1290 1656 2017 1290
Size: 366 361 3937
Fragments arranged by size:
3937 366 361
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 131 188 1248 1496 2095 2343 3045 4541 131
Size: 57 1060 248 599 248 702 1496 254
Fragments arranged by size:
1496 1060 702 599 254 248 248 57
BsaWI w'CCGG_w
Cuts at: 701 1556 1853 2374 3052 3326 3473 3884 701
Size: 855 297 521 678 274 147 411 1481
Fragments arranged by size:
1481 855 678 521 411 297 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1113 1143 1113
Size: 30 4634
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1121 1432 1435 1438 1441 1444 1447 1450 1121
Size: 311 3 3 3 3 3 3 4335
Fragments arranged by size:
4335 311 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 280 751 933 1325 2089 2847 3424 3920 280
Size: 471 182 392 764 758 577 496 1024
Fragments arranged by size:
1024 764 758 577 496 471 392 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 606 856 238
Size: 368 250 4046
Fragments arranged by size:
4046 368 250
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3460 104
Size: 2102 46 1208 1308
Fragments arranged by size:
2102 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1678 2456 2646 3438 1678
Size: 778 190 792 2904
Fragments arranged by size:
2904 792 778 190
BsmI GAATG_Cn'
Cuts at: 172 172
Size: 4664
BsmBI CGTCTCn'nnnn_
Cuts at: 1133 1133
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 752 1166 1678 2289 2382 2456 2646 2708 3438
Size: 414 512 611 93 74 190 62 730
Cuts at: 3438 752
Size: 1978
Fragments arranged by size:
1978 730 611 512 414 190 93 74
62
BspEI T'CCGG_A
Cuts at: 701 3884 701
Size: 3183 1481
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 893 919 1361 1714 1928 2230 2611 628
Size: 265 26 442 353 214 302 381 2681
Fragments arranged by size:
2681 442 381 353 302 265 214 26
BsrBI CCG'CTC
Cuts at: 50 2956 3010 4494 4659 50
Size: 2906 54 1484 165 55
Fragments arranged by size:
2906 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 1158 1250 1390 2662 2843 3052 1158
Size: 92 140 1272 181 209 2770
Fragments arranged by size:
2770 1272 209 181 140 92
BssHII G'CGCG_C
Cuts at: 1149 1149
Size: 4664
BssSI C'ACGA_G
Cuts at: 2935 3293 2935
Size: 358 4306
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 852 852
Size: 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 984 984
Size: 4664
BstYI r'GATC_y
Cuts at: 1 698 1689 2156 2179 2514 2760 3772 3858
Size: 697 991 467 23 335 246 1012 86
Cuts at: 3858 3870 4216 1
Size: 12 346 449
Fragments arranged by size:
1012 991 697 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 848 848
Size: 4664
BtgI C'CryG_G
Cuts at: 107 359 1166 1517 107
Size: 252 807 351 3254
Fragments arranged by size:
3254 807 351 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2614 2795 2958 2614
Size: 181 163 4320
Fragments arranged by size:
4320 181 163
BtsI GCAGTG_nn'
Cuts at: 255 578 1907 255
Size: 323 1329 3012
Fragments arranged by size:
3012 1329 323
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DraIII CAC_nnn'GTG
Cuts at: 1402 1402
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 341 536 614 1169 2249 2423 2814 2841 4186
Size: 195 78 555 1080 174 391 27 1345
Cuts at: 4186 341
Size: 819
Fragments arranged by size:
1345 1080 819 555 391 195 174 78
27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 1012 2687 2897 1012
Size: 1675 210 2779
Fragments arranged by size:
2779 1675 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 424 2872 3182 3328 4474 424
Size: 2448 310 146 1146 614
Fragments arranged by size:
2448 1146 614 310 146
EcoICRI GAG'CTC
Cuts at: 1676 1676
Size: 4664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 475 2489 2921 3668 3936 469
Size: 6 2014 432 747 268 1197
Fragments arranged by size:
2014 1197 747 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 606 1018 1043 1803 606
Size: 412 25 760 3467
Fragments arranged by size:
3467 760 412 25
EcoRI G'AATT_C
Cuts at: 400 1111 1668 400
Size: 711 557 3396
Fragments arranged by size:
3396 711 557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FseI GG_CCGG'CC
Cuts at: 1394 1394
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 134 1251 2098 2346 3368 4347 134
Size: 1117 847 248 1022 979 451
Fragments arranged by size:
1117 1022 979 847 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 2506 2536 2538 2568 143
Size: 32 2331 30 2 30 2239
Fragments arranged by size:
2331 2239 32 30 30 2
HincII GTy'rAC
Cuts at: 792 1703 792
Size: 911 3753
KasI G'GCGC_C
Cuts at: 130 1247 2094 2342 130
Size: 1117 847 248 2452
Fragments arranged by size:
2452 1117 847 248
KpnI G_GTAC'C
Cuts at: 566 885 1684 566
Size: 319 799 3546
Fragments arranged by size:
3546 799 319
MfeI C'AATT_G
Cuts at: 549 549
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3335 3519 3335
Size: 184 4480
MscI TGG'CCA
Cuts at: 343 538 616 2425 343
Size: 195 78 1809 2582
Fragments arranged by size:
2582 1809 195 78
MslI CAynn'nnrTG
Cuts at: 244 364 532 1292 2653 2780 244
Size: 120 168 760 1361 127 2128
Fragments arranged by size:
2128 1361 760 168 127 120
MspA1I CmG'CkG
Cuts at: 136 891 1590 1775 2089 2449 3462 3707 136
Size: 755 699 185 314 360 1013 245 1093
Fragments arranged by size:
1093 1013 755 699 360 314 245 185
NaeI GCC'GGC
Cuts at: 1252 1392 2845 1252
Size: 140 1453 3071
Fragments arranged by size:
3071 1453 140
NarI GG'CG_CC
Cuts at: 131 1248 2095 2343 131
Size: 1117 847 248 2452
Fragments arranged by size:
2452 1117 847 248
NcoI C'CATG_G
Cuts at: 107 359 107
Size: 252 4412
NgoMIV G'CCGG_C
Cuts at: 1250 1390 2843 1250
Size: 140 1453 3071
Fragments arranged by size:
3071 1453 140
NspI r_CATG'y
Cuts at: 1717 2217 3124 1717
Size: 500 907 3257
Fragments arranged by size:
3257 907 500
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PflMI CCAn_nnn'nTGG
Cuts at: 551 1402 551
Size: 851 3813
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PmlI CAC'GTG
Cuts at: 1290 1290
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 606 1018 1043 606
Size: 412 25 4227
Fragments arranged by size:
4227 412 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1342 1374 1342
Size: 32 4632
PstI C_TGCA'G
Cuts at: 262 890 1358 1711 2396 262
Size: 628 468 353 685 2530
Fragments arranged by size:
2530 685 628 468 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 1590 2089 2449 1590
Size: 499 360 3805
Fragments arranged by size:
3805 499 360
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1678 1678
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SanDI GG'GwC_CC
Cuts at: 1018 1018
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SfcI C'TryA_G
Cuts at: 33 258 886 1029 1272 1305 1354 1707 2392
Size: 225 628 143 243 33 49 353 685
Cuts at: 2392 3385 3576 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 628 353 243 225 191
143 49 33
SfoI GGC'GCC
Cuts at: 132 1249 2096 2344 132
Size: 1117 847 248 2452
Fragments arranged by size:
2452 1117 847 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 1686 1686
Size: 4664
SmlI C'TyrA_G
Cuts at: 1819 2734 3226 3488 1819
Size: 915 492 262 2995
Fragments arranged by size:
2995 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
StyI C'CwwG_G
Cuts at: 107 359 776 977 1798 2152 107
Size: 252 417 201 821 354 2619
Fragments arranged by size:
2619 821 417 354 252 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 1349 1390 2247 2380 2839 3048 310
Size: 1039 41 857 133 459 209 1926
Fragments arranged by size:
1926 1039 857 459 209 133 41
TatI w'GTAC_w
Cuts at: 4179 4179
Size: 4664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 757 789 1113 1145 4181 4213 757
Size: 32 324 32 3036 32 1208
Fragments arranged by size:
3036 1208 324 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 604 2461 604
Size: 1857 2807
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 336 2159 114
Size: 222 1823 2619
Fragments arranged by size:
2619 1823 222
XmaI C'CCGG_G
Cuts at: 1684 1684
Size: 4664
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AvrII
BaeI BbsI BcgI BfrBI BglI BmtI BsiWI BsrGI
BstZ17I ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspOMI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI