Restriction map for
pF1KB0477
(Circular) (Six-base) MAPSORT of: pF1KB0477.seq Check: 7060 from: 1 to: 4667
pF1KB0477 4667 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1096 1096
Size: 4667
AccI GT'mk_AC
Cuts at: 1705 1705
Size: 4667
Acc65I G'GTAC_C
Cuts at: 1683 1683
Size: 4667
AclI AA'CG_TT
Cuts at: 4507 4507
Size: 4667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1035 1213 2492 2924 3671 3939 631
Size: 404 178 1279 432 747 268 1359
Fragments arranged by size:
1359 1279 747 432 404 268 178
AflIII A'CryG_T
Cuts at: 773 2019 3123 4100 773
Size: 1246 1104 977 1340
Fragments arranged by size:
1340 1246 1104 977
AgeI A'CCGG_T
Cuts at: 785 3055 785
Size: 2270 2397
AleI CACnn'nnGTG
Cuts at: 1156 1156
Size: 4667
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1283 1315 1283
Size: 32 4635
AlwNI CAG_nnn'CTG
Cuts at: 393 519 609 1088 1183 1209 1972 3539 393
Size: 126 90 479 95 26 763 1567 1521
Fragments arranged by size:
1567 1521 763 479 126 95 90 26
ApaI G_GGCC'C
Cuts at: 465 465
Size: 4667
ApaLI G'TGCA_C
Cuts at: 3437 3437
Size: 4667
ApoI r'AATT_y
Cuts at: 953 1671 4020 4276 953
Size: 718 2349 256 1344
Fragments arranged by size:
2349 1344 718 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
AvaI C'yCGr_G
Cuts at: 167 665 713 1124 1687 167
Size: 498 48 411 563 3147
Fragments arranged by size:
3147 563 498 411 48
BamHI G'GATC_C
Cuts at: 1692 1692
Size: 4667
BanI G'GyrC_C
Cuts at: 885 1683 2097 2345 2380 885
Size: 798 414 248 35 3172
Fragments arranged by size:
3172 798 414 248 35
BanII G_rGCy'C
Cuts at: 335 465 816 1201 1681 2711 335
Size: 130 351 385 480 1030 2291
Fragments arranged by size:
2291 1030 480 385 351 130
BbeI G_GCGC'C
Cuts at: 2101 2349 2101
Size: 248 4419
BbvCI CC'TCA_GC
Cuts at: 275 394 275
Size: 119 4548
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3326 4667 2565
Size: 761 1341 2565
Fragments arranged by size:
2565 1341 761
BclI T'GATC_A
Cuts at: 2187 2187
Size: 4667
BglI GCCn_nnn'nGGC
Cuts at: 469 469
Size: 4667
BglII A'GATC_T
Cuts at: 984 1238 2182 4219 984
Size: 254 944 2037 1432
Fragments arranged by size:
2037 1432 944 254
BlpI GC'TnA_GC
Cuts at: 1779 1779
Size: 4667
Bme1580I G_kGCm'C
Cuts at: 465 528 644 1541 2292 2385 3441 465
Size: 63 116 897 751 93 1056 1691
Fragments arranged by size:
1691 1056 897 751 116 93 63
BmgBI CAC'GTC
Cuts at: 639 639
Size: 4667
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1879 1960 2295 4439 1303
Size: 576 81 335 2144 1531
Fragments arranged by size:
2144 1531 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 369 680 1215 3034 369
Size: 311 535 1819 2002
Fragments arranged by size:
2002 1819 535 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 445 830 1065 1210 1231 1522 1534 1585
Size: 96 385 235 145 21 291 12 51
Cuts at: 1585 349
Size: 3431
Fragments arranged by size:
3431 385 291 235 145 96 51 21
12
Bpu10I CC'TnA_GC
Cuts at: 275 394 275
Size: 119 4548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1843 2722 3214 3512 604
Size: 1239 879 492 298 1759
Fragments arranged by size:
1759 1239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4371 4371
Size: 4667
BsaAI yAC'GTr
Cuts at: 529 774 1593 2020 529
Size: 245 819 427 3176
Fragments arranged by size:
3176 819 427 245
BsaBI GATnn'nnATC
Cuts at: 6 1237 2181 4218 6
Size: 1231 944 2037 455
Fragments arranged by size:
2037 1231 944 455
BsaHI Gr'CG_yC
Cuts at: 2098 2346 3048 4544 2098
Size: 248 702 1496 2221
Fragments arranged by size:
2221 1496 702 248
BsaWI w'CCGG_w
Cuts at: 227 416 542 785 1856 2377 3055 3329 3476
Size: 189 126 243 1071 521 678 274 147
Cuts at: 3476 3887 227
Size: 411 1007
Fragments arranged by size:
1071 1007 678 521 411 274 243 189
147 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1207 1237 1283 1313 1426 1456 1207
Size: 30 46 30 113 30 4418
Fragments arranged by size:
4418 113 46 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 1228 601
Size: 627 4040
BseYI C'CCAG_C
Cuts at: 578 1251 1429 2092 2850 3427 3923 578
Size: 673 178 663 758 577 496 1322
Fragments arranged by size:
1322 758 673 663 577 496 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1365 514
Size: 851 3816
BsiEI CG_ry'CG
Cuts at: 104 2209 2255 3463 104
Size: 2105 46 1208 1308
Fragments arranged by size:
2105 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 674 1201 1535 1681 2459 2649 3441 335
Size: 339 527 334 146 778 190 792 1561
Fragments arranged by size:
1561 792 778 527 339 334 190 146
Bsp1286I G_dGCh'C
Cuts at: 335 465 528 644 674 816 1201 1535 1541
Size: 130 63 116 30 142 385 334 6
Cuts at: 1541 1681 2292 2385 2459 2649 2711 3441 335
Size: 140 611 93 74 190 62 730 1561
Fragments arranged by size:
1561 730 611 385 334 190 142 140
130 116 93 74 63 62 30 6
BspEI T'CCGG_A
Cuts at: 416 542 3887 416
Size: 126 3345 1196
Fragments arranged by size:
3345 1196 126
BspHI T'CATG_A
Cuts at: 3843 3843
Size: 4667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 608 806 1067 1096 1717 1931 2233 2614
Size: 237 198 261 29 621 214 302 381
Cuts at: 2614 371
Size: 2424
Fragments arranged by size:
2424 621 381 302 261 237 214 198
29
BsrBI CCG'CTC
Cuts at: 50 291 2959 3013 4497 4662 50
Size: 241 2668 54 1484 165 55
Fragments arranged by size:
2668 1484 241 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2579 2579
Size: 4667
BsrFI r'CCGG_y
Cuts at: 785 2665 2846 3055 785
Size: 1880 181 209 2397
Fragments arranged by size:
2397 1880 209 181
BssHII G'CGCG_C
Cuts at: 250 250
Size: 4667
BssSI C'ACGA_G
Cuts at: 1534 2938 3296 1534
Size: 1404 358 2905
Fragments arranged by size:
2905 1404 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2044 2044
Size: 4667
BstBI TT'CG_AA
Cuts at: 3028 3028
Size: 4667
BstEII G'GTnAC_C
Cuts at: 1362 1362
Size: 4667
BstXI CCAn_nnnn'nTGG
Cuts at: 484 778 484
Size: 294 4373
BstYI r'GATC_y
Cuts at: 1 413 539 984 1238 1692 2159 2182 2517
Size: 412 126 445 254 454 467 23 335
Cuts at: 2517 2763 3775 3861 3873 4219 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 454 449 445 412 346 335
254 246 126 86 23 12
BtgI C'CryG_G
Cuts at: 107 119 900 1043 107
Size: 12 781 143 3731
Fragments arranged by size:
3731 781 143 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1476 2617 2798 2961 1476
Size: 1141 181 163 3182
Fragments arranged by size:
3182 1141 181 163
BtsI GCAGTG_nn'
Cuts at: 461 914 1910 461
Size: 453 996 3218
Fragments arranged by size:
3218 996 453
DraI TTT'AAA
Cuts at: 1666 1666
Size: 4667
DraIII CAC_nnn'GTG
Cuts at: 1044 1455 1044
Size: 411 4256
DrdI GACnn_nn'nnGTC
Cuts at: 643 1063 2373 3231 643
Size: 420 1310 858 2079
Fragments arranged by size:
2079 1310 858 420
EaeI y'GGCC_r
Cuts at: 531 780 1644 2252 2426 2817 2844 4189 531
Size: 249 864 608 174 391 27 1345 1009
Fragments arranged by size:
1345 1009 864 608 391 249 174 27
EagI C'GGCC_G
Cuts at: 2252 2252
Size: 4667
EarI CTCTTCn'nnn_
Cuts at: 305 425 2690 2900 305
Size: 120 2265 210 2072
Fragments arranged by size:
2265 2072 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 373 850 2875 3185 3331 4477 373
Size: 477 2025 310 146 1146 563
Fragments arranged by size:
2025 1146 563 477 310 146
EcoICRI GAG'CTC
Cuts at: 333 1199 1679 333
Size: 866 480 3321
Fragments arranged by size:
3321 866 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 445 631 830 1035 1065 1210 1213 1231
Size: 96 186 199 205 30 145 3 18
Cuts at: 1231 1522 1534 1585 2492 2924 3671 3939 349
Size: 291 12 51 907 432 747 268 1077
Fragments arranged by size:
1077 907 747 432 291 268 205 199
186 145 96 51 30 18 12 3
EcoO109I rG'GnC_Cy
Cuts at: 462 501 1056 1180 1269 1806 462
Size: 39 555 124 89 537 3323
Fragments arranged by size:
3323 555 537 124 89 39
EcoRI G'AATT_C
Cuts at: 1671 1671
Size: 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4667
FspI TGC'GCA
Cuts at: 300 2448 300
Size: 2148 2519
HaeII r_GCGC'y
Cuts at: 132 610 765 2101 2349 3371 4350 132
Size: 478 155 1336 248 1022 979 449
Fragments arranged by size:
1336 1022 979 478 449 248 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 1207 1239 2509 2539 2541 2571 164
Size: 32 1011 32 1270 30 2 30 2260
Fragments arranged by size:
2260 1270 1011 32 32 30 30 2
HincII GTy'rAC
Cuts at: 837 1706 837
Size: 869 3798
HindIII A'AGCT_T
Cuts at: 853 853
Size: 4667
KasI G'GCGC_C
Cuts at: 2097 2345 2097
Size: 248 4419
KpnI G_GTAC'C
Cuts at: 1687 1687
Size: 4667
MluI A'CGCG_T
Cuts at: 4100 4100
Size: 4667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 209 663 732 1400 3338 3522 209
Size: 454 69 668 1938 184 1354
Fragments arranged by size:
1938 1354 668 454 184 69
MscI TGG'CCA
Cuts at: 782 1646 2428 782
Size: 864 782 3021
Fragments arranged by size:
3021 864 782
MslI CAynn'nnrTG
Cuts at: 877 883 1156 2656 2783 877
Size: 6 273 1500 127 2761
Fragments arranged by size:
2761 1500 273 127 6
MspA1I CmG'CkG
Cuts at: 279 402 442 902 1006 1206 1778 2092 2452
Size: 123 40 460 104 200 572 314 360
Cuts at: 2452 3465 3710 279
Size: 1013 245 1236
Fragments arranged by size:
1236 1013 572 460 360 314 245 200
123 104 40
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 4667
NarI GG'CG_CC
Cuts at: 2098 2346 2098
Size: 248 4419
NcoI C'CATG_G
Cuts at: 107 119 107
Size: 12 4655
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 4667
NspI r_CATG'y
Cuts at: 305 1720 2220 3127 305
Size: 1415 500 907 1845
Fragments arranged by size:
1845 1415 907 500
PasI CC'CwG_GG
Cuts at: 569 1183 569
Size: 614 4053
PciI A'CATG_T
Cuts at: 3123 3123
Size: 4667
PflMI CCAn_nnn'nTGG
Cuts at: 1044 1293 1044
Size: 249 4418
PfoI T'CCnGG_A
Cuts at: 1271 1499 1271
Size: 228 4439
PmeI GTTT'AAAC
Cuts at: 1666 1666
Size: 4667
PmlI CAC'GTG
Cuts at: 529 774 1593 529
Size: 245 819 3603
Fragments arranged by size:
3603 819 245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1283 1315 3806 3838 577
Size: 32 674 32 2491 32 1406
Fragments arranged by size:
2491 1406 674 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 501 1056 1269 501
Size: 555 213 3899
Fragments arranged by size:
3899 555 213
PspOMI G'GGCC_C
Cuts at: 461 461
Size: 4667
PspXI vC'TCGA_Gb
Cuts at: 665 665
Size: 4667
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 223 255 223
Size: 32 4635
PstI C_TGCA'G
Cuts at: 407 605 824 1442 1714 2399 407
Size: 198 219 618 272 685 2675
Fragments arranged by size:
2675 685 618 272 219 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4667
PvuII CAG'CTG
Cuts at: 279 402 442 1206 2092 2452 279
Size: 123 40 764 886 360 2494
Fragments arranged by size:
2494 886 764 360 123 40
RsrII CG'GwC_CG
Cuts at: 2862 2862
Size: 4667
SacI G_AGCT'C
Cuts at: 335 1201 1681 335
Size: 866 480 3321
Fragments arranged by size:
3321 866 480
SacII CC_GC'GG
Cuts at: 903 903
Size: 4667
SalI G'TCGA_C
Cuts at: 1704 1704
Size: 4667
SapI GCTCTTCn'nnn_
Cuts at: 2690 2900 2690
Size: 210 4457
SbfI CC_TGCA'GG
Cuts at: 605 1714 605
Size: 1109 3558
ScaI AGT'ACT
Cuts at: 4184 4184
Size: 4667
SfcI C'TryA_G
Cuts at: 33 212 261 403 601 820 1438 1710 2395
Size: 179 49 142 198 219 618 272 685
Cuts at: 2395 3388 3579 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 618 272 219 198 191
179 142 49
SfoI GGC'GCC
Cuts at: 2099 2347 2099
Size: 248 4419
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
SmaI CCC'GGG
Cuts at: 169 1126 1689 169
Size: 957 563 3147
Fragments arranged by size:
3147 957 563
SmlI C'TyrA_G
Cuts at: 583 665 1822 2737 3229 3491 583
Size: 82 1157 915 492 262 1759
Fragments arranged by size:
1759 1157 915 492 262 82
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4667
SphI G_CATG'C
Cuts at: 305 1720 2220 305
Size: 1415 500 2752
Fragments arranged by size:
2752 1415 500
StuI AGG'CCT
Cuts at: 1330 1330
Size: 4667
StyI C'CwwG_G
Cuts at: 107 119 196 1154 1801 2155 107
Size: 12 77 958 647 354 2619
Fragments arranged by size:
2619 958 647 354 77 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 647 1300 1438 2250 2383 2842 3051 647
Size: 653 138 812 133 459 209 2263
Fragments arranged by size:
2263 812 653 459 209 138 133
TatI w'GTAC_w
Cuts at: 1140 1604 4182 1140
Size: 464 2578 1625
Fragments arranged by size:
2578 1625 464
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 4635
Tth111I GACn'n_nGTC
Cuts at: 2464 2464
Size: 4667
XbaI T'CTAG_A
Cuts at: 1698 1698
Size: 4667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 727 1290 1426 2162 727
Size: 563 136 736 3232
Fragments arranged by size:
3232 736 563 136
XhoI C'TCGA_G
Cuts at: 665 665
Size: 4667
XmaI C'CCGG_G
Cuts at: 167 1124 1687 167
Size: 957 563 3147
Fragments arranged by size:
3147 957 563
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI