Restriction map for
pF1KB0479
(Circular) (Six-base) MAPSORT of: pF1KB0479.seq Check: 5391 from: 1 to: 4667
pF1KB0479 4667 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 320 320
Size: 4667
AccI GT'mk_AC
Cuts at: 1705 1705
Size: 4667
Acc65I G'GTAC_C
Cuts at: 1683 1683
Size: 4667
AclI AA'CG_TT
Cuts at: 4507 4507
Size: 4667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 774 1054 2492 2924 3671 3939 649
Size: 125 280 1438 432 747 268 1377
Fragments arranged by size:
1438 1377 747 432 280 268 125
AflIII A'CryG_T
Cuts at: 2019 3123 4100 2019
Size: 1104 977 2586
Fragments arranged by size:
2586 1104 977
AgeI A'CCGG_T
Cuts at: 3055 3055
Size: 4667
AleI CACnn'nnGTG
Cuts at: 805 805
Size: 4667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 862 1345 493
Size: 369 483 3815
Fragments arranged by size:
3815 483 369
AlwNI CAG_nnn'CTG
Cuts at: 312 496 582 1032 1518 1641 1972 3539 312
Size: 184 86 450 486 123 331 1567 1440
Fragments arranged by size:
1567 1440 486 450 331 184 123 86
ApaI G_GGCC'C
Cuts at: 750 989 750
Size: 239 4428
ApaLI G'TGCA_C
Cuts at: 3437 3437
Size: 4667
ApoI r'AATT_y
Cuts at: 1671 4020 4276 1671
Size: 2349 256 2062
Fragments arranged by size:
2349 2062 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
AvaI C'yCGr_G
Cuts at: 422 758 1609 1625 1687 422
Size: 336 851 16 62 3402
Fragments arranged by size:
3402 851 336 62 16
BamHI G'GATC_C
Cuts at: 1692 1692
Size: 4667
BanI G'GyrC_C
Cuts at: 816 876 974 1621 1683 2097 2345 2380 816
Size: 60 98 647 62 414 248 35 3103
Fragments arranged by size:
3103 647 414 248 98 62 60 35
BanII G_rGCy'C
Cuts at: 750 989 1343 1681 2711 750
Size: 239 354 338 1030 2706
Fragments arranged by size:
2706 1030 354 338 239
BbeI G_GCGC'C
Cuts at: 978 1625 2101 2349 978
Size: 647 476 248 3296
Fragments arranged by size:
3296 647 476 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 191 225 191
Size: 34 4633
BciVI GTATCCnnnnn_n'
Cuts at: 456 2565 3326 4667 456
Size: 2109 761 1341 456
Fragments arranged by size:
2109 1341 761 456
BclI T'GATC_A
Cuts at: 2187 2187
Size: 4667
BglI GCCn_nnn'nGGC
Cuts at: 735 735
Size: 4667
BglII A'GATC_T
Cuts at: 2182 4219 2182
Size: 2037 2630
BlpI GC'TnA_GC
Cuts at: 1779 1779
Size: 4667
Bme1580I G_kGCm'C
Cuts at: 115 750 948 989 1126 2292 2385 3441 115
Size: 635 198 41 137 1166 93 1056 1341
Fragments arranged by size:
1341 1166 1056 635 198 137 93 41
BmrI ACTGGGnnnn_n'
Cuts at: 135 160 1879 1960 2295 4439 135
Size: 25 1719 81 335 2144 363
Fragments arranged by size:
2144 1719 363 335 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 4667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 832 1501 1663 442
Size: 390 669 162 3446
Fragments arranged by size:
3446 669 390 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 353 1843 2722 3214 3512 285
Size: 68 1490 879 492 298 1440
Fragments arranged by size:
1490 1440 879 492 298 68
BsaI GGTCTCn'nnnn_
Cuts at: 303 1228 4371 303
Size: 925 3143 599
Fragments arranged by size:
3143 925 599
BsaAI yAC'GTr
Cuts at: 1043 2020 1043
Size: 977 3690
BsaBI GATnn'nnATC
Cuts at: 6 2181 4218 6
Size: 2175 2037 455
Fragments arranged by size:
2175 2037 455
BsaHI Gr'CG_yC
Cuts at: 554 975 1622 2098 2346 3048 4544 554
Size: 421 647 476 248 702 1496 677
Fragments arranged by size:
1496 702 677 647 476 421 248
BsaWI w'CCGG_w
Cuts at: 1145 1439 1856 2377 3055 3329 3476 3887 1145
Size: 294 417 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 417 411 294 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 508 538 1225 1255 1543 1573 508
Size: 30 687 30 288 30 3602
Fragments arranged by size:
3602 687 288 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1288 1507 1288
Size: 219 4448
BseYI C'CCAG_C
Cuts at: 225 269 670 1307 2092 2850 3427 3923 225
Size: 44 401 637 785 758 577 496 969
Fragments arranged by size:
969 785 758 637 577 496 401 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 1504
Size: 4667
BsiEI CG_ry'CG
Cuts at: 104 742 2209 2255 3463 104
Size: 638 1467 46 1208 1308
Fragments arranged by size:
1467 1308 1208 638 46
BsiHKAI G_wGCw'C
Cuts at: 1343 1681 2459 2649 3441 1343
Size: 338 778 190 792 2569
Fragments arranged by size:
2569 792 778 338 190
Bsp1286I G_dGCh'C
Cuts at: 115 750 948 989 1126 1343 1681 2292 2385
Size: 635 198 41 137 217 338 611 93
Cuts at: 2385 2459 2649 2711 3441 115
Size: 74 190 62 730 1341
Fragments arranged by size:
1341 730 635 611 338 217 198 190
137 93 74 62 41
BspEI T'CCGG_A
Cuts at: 1145 3887 1145
Size: 2742 1925
BspHI T'CATG_A
Cuts at: 1529 3843 1529
Size: 2314 2353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 1717 1931 2233 2614 320
Size: 1397 214 302 381 2373
Fragments arranged by size:
2373 1397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 519 1133 1545 2959 3013 4497 4662 50
Size: 469 614 412 1414 54 1484 165 55
Fragments arranged by size:
1484 1414 614 469 412 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1395 2579 1395
Size: 1184 3483
BsrFI r'CCGG_y
Cuts at: 1384 2665 2846 3055 1384
Size: 1281 181 209 2996
Fragments arranged by size:
2996 1281 209 181
BssSI C'ACGA_G
Cuts at: 2938 3296 2938
Size: 358 4309
BstAPI GCAn_nnn'nTGC
Cuts at: 312 1518 2044 312
Size: 1206 526 2935
Fragments arranged by size:
2935 1206 526
BstBI TT'CG_AA
Cuts at: 3028 3028
Size: 4667
BstXI CCAn_nnnn'nTGG
Cuts at: 1245 1245
Size: 4667
BstYI r'GATC_y
Cuts at: 1 1436 1692 2159 2182 2517 2763 3775 3861
Size: 1435 256 467 23 335 246 1012 86
Cuts at: 3861 3873 4219 1
Size: 12 346 449
Fragments arranged by size:
1435 1012 467 449 346 335 256 246
86 23 12
BtgI C'CryG_G
Cuts at: 380 438 612 966 380
Size: 58 174 354 4081
Fragments arranged by size:
4081 354 174 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 545 2617 2798 2961 545
Size: 2072 181 163 2251
Fragments arranged by size:
2251 2072 181 163
BtsI GCAGTG_nn'
Cuts at: 207 322 946 1910 207
Size: 115 624 964 2964
Fragments arranged by size:
2964 964 624 115
DraI TTT'AAA
Cuts at: 1666 1666
Size: 4667
DraIII CAC_nnn'GTG
Cuts at: 493 493
Size: 4667
DrdI GACnn_nn'nnGTC
Cuts at: 2373 3231 2373
Size: 858 3809
EaeI y'GGCC_r
Cuts at: 739 854 1472 2252 2426 2817 2844 4189 739
Size: 115 618 780 174 391 27 1345 1217
Fragments arranged by size:
1345 1217 780 618 391 174 115 27
EagI C'GGCC_G
Cuts at: 739 2252 739
Size: 1513 3154
EarI CTCTTCn'nnn_
Cuts at: 1003 2690 2900 1003
Size: 1687 210 2770
Fragments arranged by size:
2770 1687 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 376 1551 2875 3185 3331 4477 376
Size: 1175 1324 310 146 1146 566
Fragments arranged by size:
1324 1175 1146 566 310 146
EcoICRI GAG'CTC
Cuts at: 1341 1679 1341
Size: 338 4329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 649 774 832 1054 1501 1663 2492 2924
Size: 207 125 58 222 447 162 829 432
Cuts at: 2924 3671 3939 442
Size: 747 268 1170
Fragments arranged by size:
1170 829 747 447 432 268 222 207
162 125 58
EcoO109I rG'GnC_Cy
Cuts at: 162 230 543 617 728 746 986 1606 1638
Size: 68 313 74 111 18 240 620 32
Cuts at: 1638 1806 162
Size: 168 3023
Fragments arranged by size:
3023 620 313 240 168 111 74 68
32 18
EcoRI G'AATT_C
Cuts at: 1671 1671
Size: 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1143 2686 1143
Size: 1543 3124
FspI TGC'GCA
Cuts at: 2448 2448
Size: 4667
HaeII r_GCGC'y
Cuts at: 978 1294 1625 2101 2349 3371 4350 978
Size: 316 331 476 248 1022 979 1295
Fragments arranged by size:
1295 1022 979 476 331 316 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 508 540 544 576 1264 1296 1543 1575 2509
Size: 32 4 32 688 32 247 32 934
Cuts at: 2509 2539 2541 2571 508
Size: 30 2 30 2604
Fragments arranged by size:
2604 934 688 247 32 32 32 32
30 30 4 2
HincII GTy'rAC
Cuts at: 1706 1706
Size: 4667
KasI G'GCGC_C
Cuts at: 974 1621 2097 2345 974
Size: 647 476 248 3296
Fragments arranged by size:
3296 647 476 248
KpnI G_GTAC'C
Cuts at: 1687 1687
Size: 4667
MluI A'CGCG_T
Cuts at: 4100 4100
Size: 4667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 156 530 3338 3522 156
Size: 374 2808 184 1301
Fragments arranged by size:
2808 1301 374 184
MscI TGG'CCA
Cuts at: 2428 2428
Size: 4667
MslI CAynn'nnrTG
Cuts at: 805 2656 2783 805
Size: 1851 127 2689
Fragments arranged by size:
2689 1851 127
MspA1I CmG'CkG
Cuts at: 440 852 873 1089 1353 1471 1778 2092 2452
Size: 412 21 216 264 118 307 314 360
Cuts at: 2452 3465 3710 440
Size: 1013 245 1397
Fragments arranged by size:
1397 1013 412 360 314 307 264 245
216 118 21
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 4667
NarI GG'CG_CC
Cuts at: 975 1622 2098 2346 975
Size: 647 476 248 3296
Fragments arranged by size:
3296 647 476 248
NcoI C'CATG_G
Cuts at: 380 612 966 380
Size: 232 354 4081
Fragments arranged by size:
4081 354 232
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 4667
NspI r_CATG'y
Cuts at: 605 1720 2220 3127 605
Size: 1115 500 907 2145
Fragments arranged by size:
2145 1115 907 500
PasI CC'CwG_GG
Cuts at: 576 732 576
Size: 156 4511
PciI A'CATG_T
Cuts at: 3123 3123
Size: 4667
PflMI CCAn_nnn'nTGG
Cuts at: 381 582 722 1641 381
Size: 201 140 919 3407
Fragments arranged by size:
3407 919 201 140
PfoI T'CCnGG_A
Cuts at: 458 1220 1478 458
Size: 762 258 3647
Fragments arranged by size:
3647 762 258
PmeI GTTT'AAAC
Cuts at: 1666 1666
Size: 4667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4635
PpuMI rG'GwC_Cy
Cuts at: 162 543 162
Size: 381 4286
PshAI GACnn'nnGTC
Cuts at: 382 382
Size: 4667
PspOMI G'GGCC_C
Cuts at: 746 985 746
Size: 239 4428
PstI C_TGCA'G
Cuts at: 851 941 1514 1714 2399 851
Size: 90 573 200 685 3119
Fragments arranged by size:
3119 685 573 200 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4667
PvuII CAG'CTG
Cuts at: 852 1089 1353 2092 2452 852
Size: 237 264 739 360 3067
Fragments arranged by size:
3067 739 360 264 237
RsrII CG'GwC_CG
Cuts at: 2862 2862
Size: 4667
SacI G_AGCT'C
Cuts at: 1343 1681 1343
Size: 338 4329
SacII CC_GC'GG
Cuts at: 441 441
Size: 4667
SalI G'TCGA_C
Cuts at: 1704 1704
Size: 4667
SanDI GG'GwC_CC
Cuts at: 162 162
Size: 4667
SapI GCTCTTCn'nnn_
Cuts at: 1003 2690 2900 1003
Size: 1687 210 2770
Fragments arranged by size:
2770 1687 210
SbfI CC_TGCA'GG
Cuts at: 1514 1714 1514
Size: 200 4467
ScaI AGT'ACT
Cuts at: 4184 4184
Size: 4667
SexAI A'CCwGG_T
Cuts at: 489 489
Size: 4667
SfcI C'TryA_G
Cuts at: 33 687 847 937 1510 1710 2395 3388 3579
Size: 654 160 90 573 200 685 993 191
Cuts at: 3579 33
Size: 1121
Fragments arranged by size:
1121 993 685 654 573 200 191 160
90
SfoI GGC'GCC
Cuts at: 976 1623 2099 2347 976
Size: 647 476 248 3296
Fragments arranged by size:
3296 647 476 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
SmaI CCC'GGG
Cuts at: 760 1627 1689 760
Size: 867 62 3738
Fragments arranged by size:
3738 867 62
SmlI C'TyrA_G
Cuts at: 300 368 422 1822 2737 3229 3491 300
Size: 68 54 1400 915 492 262 1476
Fragments arranged by size:
1476 1400 915 492 262 68 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4667
SphI G_CATG'C
Cuts at: 1720 2220 1720
Size: 500 4167
StyI C'CwwG_G
Cuts at: 380 612 716 966 1801 2155 380
Size: 232 104 250 835 354 2892
Fragments arranged by size:
2892 835 354 250 232 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 378 2250 2383 2842 3051 378
Size: 1872 133 459 209 1994
Fragments arranged by size:
1994 1872 459 209 133
TatI w'GTAC_w
Cuts at: 4182 4182
Size: 4667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 4635
Tth111I GACn'n_nGTC
Cuts at: 2464 2464
Size: 4667
XbaI T'CTAG_A
Cuts at: 1698 1698
Size: 4667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1111 2162 1111
Size: 1051 3616
XhoI C'TCGA_G
Cuts at: 422 422
Size: 4667
XmaI C'CCGG_G
Cuts at: 758 1625 1687 758
Size: 867 62 3738
Fragments arranged by size:
3738 867 62
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI