Restriction map for
pF1KB0481
(Circular) (Six-base) MAPSORT of: pF1KB0481.seq Check: 7832 from: 1 to: 4676
pF1KB0481 4676 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AatII G_ACGT'C
Cuts at: 1102 1102
Size: 4676
AccI GT'mk_AC
Cuts at: 1714 1714
Size: 4676
Acc65I G'GTAC_C
Cuts at: 1692 1692
Size: 4676
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 1165 1539 2501 2933 3680 3948 440
Size: 725 374 962 432 747 268 1168
Fragments arranged by size:
1168 962 747 725 432 374 268
AflIII A'CryG_T
Cuts at: 2028 3132 4109 2028
Size: 1104 977 2595
Fragments arranged by size:
2595 1104 977
AgeI A'CCGG_T
Cuts at: 3064 3064
Size: 4676
AhdI GACnn_n'nnGTC
Cuts at: 1098 1098
Size: 4676
AlwNI CAG_nnn'CTG
Cuts at: 1494 1981 3548 1494
Size: 487 1567 2622
Fragments arranged by size:
2622 1567 487
ApaI G_GGCC'C
Cuts at: 454 454
Size: 4676
ApaLI G'TGCA_C
Cuts at: 3446 3446
Size: 4676
ApoI r'AATT_y
Cuts at: 348 1407 1680 4029 4285 348
Size: 1059 273 2349 256 739
Fragments arranged by size:
2349 1059 739 273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 632 690 790 1696 632
Size: 58 100 906 3612
Fragments arranged by size:
3612 906 100 58
BamHI G'GATC_C
Cuts at: 1701 1701
Size: 4676
BanI G'GyrC_C
Cuts at: 201 1187 1588 1692 2106 2354 2389 201
Size: 986 401 104 414 248 35 2488
Fragments arranged by size:
2488 986 414 401 248 104 35
BanII G_rGCy'C
Cuts at: 454 679 1385 1690 2720 454
Size: 225 706 305 1030 2410
Fragments arranged by size:
2410 1030 706 305 225
BbeI G_GCGC'C
Cuts at: 2110 2358 2110
Size: 248 4428
BbsI GAAGACnn'nnnn_
Cuts at: 115 115
Size: 4676
BbvCI CC'TCA_GC
Cuts at: 1576 1576
Size: 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4642
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 2196 2196
Size: 4676
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4676
BglII A'GATC_T
Cuts at: 2191 4228 2191
Size: 2037 2639
BlpI GC'TnA_GC
Cuts at: 1429 1511 1788 1429
Size: 82 277 4317
Fragments arranged by size:
4317 277 82
Bme1580I G_kGCm'C
Cuts at: 174 206 319 454 1591 2301 2394 3450 174
Size: 32 113 135 1137 710 93 1056 1400
Fragments arranged by size:
1400 1137 1056 710 135 113 93 32
BmgBI CAC'GTC
Cuts at: 921 921
Size: 4676
BmrI ACTGGGnnnn_n'
Cuts at: 972 1888 1969 2304 4448 972
Size: 916 81 335 2144 1200
Fragments arranged by size:
2144 1200 916 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 279 3043 279
Size: 2764 1912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 572 793 1567 324
Size: 248 221 774 3433
Fragments arranged by size:
3433 774 248 221
Bpu10I CC'TnA_GC
Cuts at: 1576 1576
Size: 4676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1852 2731 3223 3521 1852
Size: 879 492 298 3007
Fragments arranged by size:
3007 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 504 4380 504
Size: 3876 800
BsaAI yAC'GTr
Cuts at: 2029 2029
Size: 4676
BsaBI GATnn'nnATC
Cuts at: 6 1525 2190 4227 6
Size: 1519 665 2037 455
Fragments arranged by size:
2037 1519 665 455
BsaHI Gr'CG_yC
Cuts at: 192 1099 2107 2355 3057 4553 192
Size: 907 1008 248 702 1496 315
Fragments arranged by size:
1496 1008 907 702 315 248
BsaWI w'CCGG_w
Cuts at: 679 1457 1865 2386 3064 3338 3485 3896 679
Size: 778 408 521 678 274 147 411 1459
Fragments arranged by size:
1459 778 678 521 411 408 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1261 1291 1261
Size: 30 4646
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1225 1225
Size: 4676
BseYI C'CCAG_C
Cuts at: 186 797 1475 1664 2101 2859 3436 3932 186
Size: 611 678 189 437 758 577 496 930
Fragments arranged by size:
930 758 678 611 577 496 437 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1171 637
Size: 534 4142
BsiEI CG_ry'CG
Cuts at: 104 2218 2264 3472 104
Size: 2114 46 1208 1308
Fragments arranged by size:
2114 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1690 2468 2658 3450 1690
Size: 778 190 792 2916
Fragments arranged by size:
2916 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 199 304 199
Size: 105 4571
Bsp1286I G_dGCh'C
Cuts at: 174 206 319 454 679 1385 1591 1690 2301
Size: 32 113 135 225 706 206 99 611
Cuts at: 2301 2394 2468 2658 2720 3450 174
Size: 93 74 190 62 730 1400
Fragments arranged by size:
1400 730 706 611 225 206 190 135
113 99 93 74 62 32
BspEI T'CCGG_A
Cuts at: 679 1457 3896 679
Size: 778 2439 1459
Fragments arranged by size:
2439 1459 778
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1659 1726 1940 2242 2623 1659
Size: 67 214 302 381 3712
Fragments arranged by size:
3712 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2968 3022 4506 4671 50
Size: 2918 54 1484 165 55
Fragments arranged by size:
2918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2588 2588
Size: 4676
BsrFI r'CCGG_y
Cuts at: 2674 2855 3064 2674
Size: 181 209 4286
Fragments arranged by size:
4286 209 181
BssSI C'ACGA_G
Cuts at: 2947 3305 2947
Size: 358 4318
BstAPI GCAn_nnn'nTGC
Cuts at: 1158 2053 1158
Size: 895 3781
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstXI CCAn_nnnn'nTGG
Cuts at: 361 1347 1424 361
Size: 986 77 3613
Fragments arranged by size:
3613 986 77
BstYI r'GATC_y
Cuts at: 1 257 1701 2168 2191 2526 2772 3784 3870
Size: 256 1444 467 23 335 246 1012 86
Cuts at: 3870 3882 4228 1
Size: 12 346 449
Fragments arranged by size:
1444 1012 467 449 346 335 256 246
86 23 12
BtgI C'CryG_G
Cuts at: 1002 1002
Size: 4676
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1595 2626 2807 2970 1595
Size: 1031 181 163 3301
Fragments arranged by size:
3301 1031 181 163
BtsI GCAGTG_nn'
Cuts at: 632 1120 1495 1919 632
Size: 488 375 424 3389
Fragments arranged by size:
3389 488 424 375
DraI TTT'AAA
Cuts at: 1675 1675
Size: 4676
DrdI GACnn_nn'nnGTC
Cuts at: 2382 3240 2382
Size: 858 3818
EaeI y'GGCC_r
Cuts at: 113 1208 1358 2261 2435 2826 2853 4198 113
Size: 1095 150 903 174 391 27 1345 591
Fragments arranged by size:
1345 1095 903 591 391 174 150 27
EagI C'GGCC_G
Cuts at: 2261 2261
Size: 4676
EarI CTCTTCn'nnn_
Cuts at: 2699 2909 2699
Size: 210 4466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1518 2884 3194 3340 4486 1518
Size: 1366 310 146 1146 1708
Fragments arranged by size:
1708 1366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1688 1688
Size: 4676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 440 572 793 1165 1539 1567 2501 2933
Size: 116 132 221 372 374 28 934 432
Cuts at: 2933 3680 3948 324
Size: 747 268 1052
Fragments arranged by size:
1052 934 747 432 374 372 268 221
132 116 28
EcoNI CCTnn'n_nnAGG
Cuts at: 571 571
Size: 4676
EcoO109I rG'GnC_Cy
Cuts at: 450 451 1815 450
Size: 1 1364 3311
Fragments arranged by size:
3311 1364 1
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
EcoRV GAT'ATC
Cuts at: 1297 1297
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 349 1059 2695 349
Size: 710 1636 2330
Fragments arranged by size:
2330 1636 710
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 762 2110 2358 3380 4359 762
Size: 1348 248 1022 979 1079
Fragments arranged by size:
1348 1079 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 249 281 458 490 613 645 1084
Size: 32 8 32 177 32 123 32 439
Cuts at: 1084 1116 1490 1522 1550 1582 2518 2548 2550
Size: 32 374 32 28 32 936 30 2
Cuts at: 2550 2580 209
Size: 30 2305
Fragments arranged by size:
2305 936 439 374 177 123 32 32
32 32 32 32 32 30 30 28
8 2
HincII GTy'rAC
Cuts at: 1715 1715
Size: 4676
KasI G'GCGC_C
Cuts at: 2106 2354 2106
Size: 248 4428
KpnI G_GTAC'C
Cuts at: 1696 1696
Size: 4676
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 599 1593 3347 3531 599
Size: 994 1754 184 1744
Fragments arranged by size:
1754 1744 994 184
MscI TGG'CCA
Cuts at: 115 1210 1360 2437 115
Size: 1095 150 1077 2354
Fragments arranged by size:
2354 1095 1077 150
MslI CAynn'nnrTG
Cuts at: 1072 1124 1279 1540 1609 2665 2792 1072
Size: 52 155 261 69 1056 127 2956
Fragments arranged by size:
2956 1056 261 155 127 69 52
MspA1I CmG'CkG
Cuts at: 1224 1787 2101 2461 3474 3719 1224
Size: 563 314 360 1013 245 2181
Fragments arranged by size:
2181 1013 563 360 314 245
NaeI GCC'GGC
Cuts at: 2857 2857
Size: 4676
NarI GG'CG_CC
Cuts at: 2107 2355 2107
Size: 248 4428
NgoMIV G'CCGG_C
Cuts at: 2855 2855
Size: 4676
NspI r_CATG'y
Cuts at: 1729 2229 3136 1729
Size: 500 907 3269
Fragments arranged by size:
3269 907 500
PasI CC'CwG_GG
Cuts at: 176 482 1197 176
Size: 306 715 3655
Fragments arranged by size:
3655 715 306
PciI A'CATG_T
Cuts at: 3132 3132
Size: 4676
PflMI CCAn_nnn'nTGG
Cuts at: 893 893
Size: 4676
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 455 487 3815 3847 455
Size: 32 3328 32 1284
Fragments arranged by size:
3328 1284 32 32
PspOMI G'GGCC_C
Cuts at: 450 450
Size: 4676
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1435 1467 1435
Size: 32 4644
PstI C_TGCA'G
Cuts at: 1115 1292 1723 2408 1115
Size: 177 431 685 3383
Fragments arranged by size:
3383 685 431 177
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4676
PvuII CAG'CTG
Cuts at: 1224 2101 2461 1224
Size: 877 360 3439
Fragments arranged by size:
3439 877 360
RsrII CG'GwC_CG
Cuts at: 2871 2871
Size: 4676
SacI G_AGCT'C
Cuts at: 1690 1690
Size: 4676
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SapI GCTCTTCn'nnn_
Cuts at: 2699 2909 2699
Size: 210 4466
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 4193 4193
Size: 4676
SfcI C'TryA_G
Cuts at: 33 1014 1111 1219 1288 1719 2404 3397 3588
Size: 981 97 108 69 431 685 993 191
Cuts at: 3588 33
Size: 1121
Fragments arranged by size:
1121 993 981 685 431 191 108 97
69
SfoI GGC'GCC
Cuts at: 2108 2356 2108
Size: 248 4428
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SmaI CCC'GGG
Cuts at: 1698 1698
Size: 4676
SmlI C'TyrA_G
Cuts at: 1831 2746 3238 3500 1831
Size: 915 492 262 3007
Fragments arranged by size:
3007 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 1729 2229 1729
Size: 500 4176
StyI C'CwwG_G
Cuts at: 116 1810 2164 116
Size: 1694 354 2628
Fragments arranged by size:
2628 1694 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 964 2259 2392 2851 3060 964
Size: 1295 133 459 209 2580
Fragments arranged by size:
2580 1295 459 209 133
TatI w'GTAC_w
Cuts at: 4191 4191
Size: 4676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 222 254 311 343 1261 1293 4193
Size: 32 68 32 57 32 918 32 2900
Cuts at: 4193 4225 122
Size: 32 573
Fragments arranged by size:
2900 918 573 68 57 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 640 2473 640
Size: 1833 2843
XbaI T'CTAG_A
Cuts at: 1707 1707
Size: 4676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1000 1368 2171 183
Size: 817 368 803 2688
Fragments arranged by size:
2688 817 803 368
XmaI C'CCGG_G
Cuts at: 1696 1696
Size: 4676
ZraI GAC'GTC
Cuts at: 1100 1100
Size: 4676
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI