Restriction map for
pF1KB0485
(Circular) (Six-base) MAPSORT of: pF1KB0485.seq Check: 1344 from: 1 to: 4697
pF1KB0485 4697 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1102 1397 1102
Size: 295 4402
AccI GT'mk_AC
Cuts at: 1735 1735
Size: 4697
Acc65I G'GTAC_C
Cuts at: 1713 1713
Size: 4697
AclI AA'CG_TT
Cuts at: 4537 4537
Size: 4697
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 2954 3701 3969 2522
Size: 432 747 268 3250
Fragments arranged by size:
3250 747 432 268
AflIII A'CryG_T
Cuts at: 2049 3153 4130 2049
Size: 1104 977 2616
Fragments arranged by size:
2616 1104 977
AgeI A'CCGG_T
Cuts at: 3085 3085
Size: 4697
AhdI GACnn_n'nnGTC
Cuts at: 779 779
Size: 4697
AleI CACnn'nnGTG
Cuts at: 952 1666 952
Size: 714 3983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 196 228 196
Size: 32 4665
AlwNI CAG_nnn'CTG
Cuts at: 172 1602 2002 3569 172
Size: 1430 400 1567 1300
Fragments arranged by size:
1567 1430 1300 400
ApaLI G'TGCA_C
Cuts at: 3467 3467
Size: 4697
ApoI r'AATT_y
Cuts at: 1701 4050 4306 1701
Size: 2349 256 2092
Fragments arranged by size:
2349 2092 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4697
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4697
AvaI C'yCGr_G
Cuts at: 180 223 575 1163 1717 180
Size: 43 352 588 554 3160
Fragments arranged by size:
3160 588 554 352 43
BamHI G'GATC_C
Cuts at: 1722 1722
Size: 4697
BanI G'GyrC_C
Cuts at: 175 441 1546 1641 1713 2127 2375 2410 175
Size: 266 1105 95 72 414 248 35 2462
Fragments arranged by size:
2462 1105 414 266 248 95 72 35
BanII G_rGCy'C
Cuts at: 224 404 765 906 973 990 1204 1411 1485
Size: 180 361 141 67 17 214 207 74
Cuts at: 1485 1711 2741 224
Size: 226 1030 2180
Fragments arranged by size:
2180 1030 361 226 214 207 180 141
74 67 17
BbeI G_GCGC'C
Cuts at: 2131 2379 2131
Size: 248 4449
BbsI GAAGACnn'nnnn_
Cuts at: 926 926
Size: 4697
BbvCI CC'TCA_GC
Cuts at: 136 136
Size: 4697
BciVI GTATCCnnnnn_n'
Cuts at: 2595 3356 4697 2595
Size: 761 1341 2595
Fragments arranged by size:
2595 1341 761
BclI T'GATC_A
Cuts at: 2217 2217
Size: 4697
BglI GCCn_nnn'nGGC
Cuts at: 1199 1312 1199
Size: 113 4584
BglII A'GATC_T
Cuts at: 894 2212 4249 894
Size: 1318 2037 1342
Fragments arranged by size:
2037 1342 1318
BlpI GC'TnA_GC
Cuts at: 670 1809 670
Size: 1139 3558
Bme1580I G_kGCm'C
Cuts at: 1524 1549 1584 1646 1673 2322 2415 3471 1524
Size: 25 35 62 27 649 93 1056 2750
Fragments arranged by size:
2750 1056 649 93 62 35 27 25
BmrI ACTGGGnnnn_n'
Cuts at: 535 768 1311 1534 1909 1990 2325 4469 535
Size: 233 543 223 375 81 335 2144 763
Fragments arranged by size:
2144 763 543 375 335 233 223 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 870 1303 3064 870
Size: 433 1761 2503
Fragments arranged by size:
2503 1761 433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 767 408
Size: 359 4338
Bpu10I CC'TnA_GC
Cuts at: 136 136
Size: 4697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1873 2752 3244 3542 1873
Size: 879 492 298 3028
Fragments arranged by size:
3028 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1021 1276 4401 1021
Size: 255 3125 1317
Fragments arranged by size:
3125 1317 255
BsaAI yAC'GTr
Cuts at: 1332 1593 2050 1332
Size: 261 457 3979
Fragments arranged by size:
3979 457 261
BsaBI GATnn'nnATC
Cuts at: 6 2211 4248 6
Size: 2205 2037 455
Fragments arranged by size:
2205 2037 455
BsaHI Gr'CG_yC
Cuts at: 350 2128 2376 3078 4574 350
Size: 1778 248 702 1496 473
Fragments arranged by size:
1778 1496 702 473 248
BsaWI w'CCGG_w
Cuts at: 693 1102 1631 1886 2407 3085 3359 3506 3917
Size: 409 529 255 521 678 274 147 411
Cuts at: 3917 693
Size: 1473
Fragments arranged by size:
1473 678 529 521 411 409 274 255
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 556 232
Size: 324 4373
BseYI C'CCAG_C
Cuts at: 1392 1402 1646 2122 2880 3457 3953 1392
Size: 10 244 476 758 577 496 2136
Fragments arranged by size:
2136 758 577 496 476 244 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1573 1591 1615 1190
Size: 383 18 24 4272
Fragments arranged by size:
4272 383 24 18
BsiEI CG_ry'CG
Cuts at: 104 276 1572 1632 2239 2285 3493 104
Size: 172 1296 60 607 46 1208 1308
Fragments arranged by size:
1308 1296 1208 607 172 60 46
BsiHKAI G_wGCw'C
Cuts at: 1688 1711 2489 2679 3471 1688
Size: 23 778 190 792 2914
Fragments arranged by size:
2914 792 778 190 23
BsmI GAATG_Cn'
Cuts at: 994 994
Size: 4697
Bsp1286I G_dGCh'C
Cuts at: 224 404 765 906 973 990 1204 1411 1485
Size: 180 361 141 67 17 214 207 74
Cuts at: 1485 1524 1549 1584 1646 1673 1688 1711 2322
Size: 39 25 35 62 27 15 23 611
Cuts at: 2322 2415 2489 2679 2741 3471 224
Size: 93 74 190 62 730 1450
Fragments arranged by size:
1450 730 611 361 214 207 190 180
141 93 74 74 67 62 62 39
35 27 25 23 17 15
BspEI T'CCGG_A
Cuts at: 3917 3917
Size: 4697
BspHI T'CATG_A
Cuts at: 3873 3873
Size: 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1102 1397 1747 1961 2263 2644 1102
Size: 295 350 214 302 381 3155
Fragments arranged by size:
3155 381 350 302 295 214
BsrBI CCG'CTC
Cuts at: 50 1658 2989 3043 4527 4692 50
Size: 1608 1331 54 1484 165 55
Fragments arranged by size:
1608 1484 1331 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 629 716 2609 500
Size: 129 87 1893 2588
Fragments arranged by size:
2588 1893 129 87
BsrFI r'CCGG_y
Cuts at: 259 1142 1574 2695 2876 3085 259
Size: 883 432 1121 181 209 1871
Fragments arranged by size:
1871 1121 883 432 209 181
BssSI C'ACGA_G
Cuts at: 882 2968 3326 882
Size: 2086 358 2253
Fragments arranged by size:
2253 2086 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1602 2074 1602
Size: 472 4225
BstBI TT'CG_AA
Cuts at: 3058 3058
Size: 4697
BstXI CCAn_nnnn'nTGG
Cuts at: 432 696 432
Size: 264 4433
BstYI r'GATC_y
Cuts at: 1 894 1722 2189 2212 2547 2793 3805 3891
Size: 893 828 467 23 335 246 1012 86
Cuts at: 3891 3903 4249 1
Size: 12 346 449
Fragments arranged by size:
1012 893 828 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 197 341 842 1239 1673 107
Size: 90 144 501 397 434 3131
Fragments arranged by size:
3131 501 434 397 144 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 600 2647 2828 2991 600
Size: 2047 181 163 2306
Fragments arranged by size:
2306 2047 181 163
BtsI GCAGTG_nn'
Cuts at: 708 947 1940 708
Size: 239 993 3465
Fragments arranged by size:
3465 993 239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 612 647 612
Size: 35 4662
DraI TTT'AAA
Cuts at: 1696 1696
Size: 4697
DraIII CAC_nnn'GTG
Cuts at: 884 884
Size: 4697
DrdI GACnn_nn'nnGTC
Cuts at: 528 1290 2403 3261 528
Size: 762 1113 858 1964
Fragments arranged by size:
1964 1113 858 762
EaeI y'GGCC_r
Cuts at: 110 273 647 794 1253 1313 1676 2282 2456
Size: 163 374 147 459 60 363 606 174
Cuts at: 2456 2847 2874 4219 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 606 588 459 391 374 363 174
163 147 60 27
EagI C'GGCC_G
Cuts at: 273 2282 273
Size: 2009 2688
EarI CTCTTCn'nnn_
Cuts at: 1065 1222 2720 2930 1065
Size: 157 1498 210 2832
Fragments arranged by size:
2832 1498 210 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 1138 2905 3215 3361 4507 160
Size: 978 1767 310 146 1146 350
Fragments arranged by size:
1767 1146 978 350 310 146
EcoICRI GAG'CTC
Cuts at: 1709 1709
Size: 4697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 767 2522 2954 3701 3969 408
Size: 359 1755 432 747 268 1136
Fragments arranged by size:
1755 1136 747 432 359 268
EcoNI CCTnn'n_nnAGG
Cuts at: 139 139
Size: 4697
EcoO109I rG'GnC_Cy
Cuts at: 228 579 1599 1836 228
Size: 351 1020 237 3089
Fragments arranged by size:
3089 1020 351 237
EcoRI G'AATT_C
Cuts at: 1701 1701
Size: 4697
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4697
FspI TGC'GCA
Cuts at: 2478 2478
Size: 4697
HaeII r_GCGC'y
Cuts at: 852 2131 2379 3401 4380 852
Size: 1279 248 1022 979 1169
Fragments arranged by size:
1279 1169 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 765 797 1042 1074 2539 2569 2571
Size: 32 354 32 245 32 1465 30 2
Cuts at: 2571 2601 379
Size: 30 2475
Fragments arranged by size:
2475 1465 354 245 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1736 1736
Size: 4697
KasI G'GCGC_C
Cuts at: 2127 2375 2127
Size: 248 4449
KpnI G_GTAC'C
Cuts at: 1717 1717
Size: 4697
MluI A'CGCG_T
Cuts at: 4130 4130
Size: 4697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 1181 3368 3552 143
Size: 1038 2187 184 1288
Fragments arranged by size:
2187 1288 1038 184
MscI TGG'CCA
Cuts at: 1255 1678 2458 1255
Size: 423 780 3494
Fragments arranged by size:
3494 780 423
MslI CAynn'nnrTG
Cuts at: 694 952 1666 2686 2813 694
Size: 258 714 1020 127 2578
Fragments arranged by size:
2578 1020 714 258 127
MspA1I CmG'CkG
Cuts at: 657 1587 1650 1808 2122 2482 3495 3740 657
Size: 930 63 158 314 360 1013 245 1614
Fragments arranged by size:
1614 1013 930 360 314 245 158 63
NaeI GCC'GGC
Cuts at: 1144 1576 2878 1144
Size: 432 1302 2963
Fragments arranged by size:
2963 1302 432
NarI GG'CG_CC
Cuts at: 2128 2376 2128
Size: 248 4449
NcoI C'CATG_G
Cuts at: 107 107
Size: 4697
NgoMIV G'CCGG_C
Cuts at: 1142 1574 2876 1142
Size: 432 1302 2963
Fragments arranged by size:
2963 1302 432
NspI r_CATG'y
Cuts at: 1750 2250 3157 1750
Size: 500 907 3290
Fragments arranged by size:
3290 907 500
PasI CC'CwG_GG
Cuts at: 939 939
Size: 4697
PciI A'CATG_T
Cuts at: 3153 3153
Size: 4697
PflMI CCAn_nnn'nTGG
Cuts at: 172 884 1301 1392 172
Size: 712 417 91 3477
Fragments arranged by size:
3477 712 417 91
PfoI T'CCnGG_A
Cuts at: 230 1044 230
Size: 814 3883
PmeI GTTT'AAAC
Cuts at: 1696 1696
Size: 4697
PmlI CAC'GTG
Cuts at: 1593 1593
Size: 4697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 865 897 3836 3868 865
Size: 32 2939 32 1694
Fragments arranged by size:
2939 1694 32 32
PpuMI rG'GwC_Cy
Cuts at: 228 228
Size: 4697
PshAI GACnn'nnGTC
Cuts at: 1105 1105
Size: 4697
PstI C_TGCA'G
Cuts at: 251 1490 1744 2429 251
Size: 1239 254 685 2519
Fragments arranged by size:
2519 1239 685 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4697
PvuII CAG'CTG
Cuts at: 1587 2122 2482 1587
Size: 535 360 3802
Fragments arranged by size:
3802 535 360
RsrII CG'GwC_CG
Cuts at: 2892 2892
Size: 4697
SacI G_AGCT'C
Cuts at: 1711 1711
Size: 4697
SalI G'TCGA_C
Cuts at: 1734 1734
Size: 4697
SapI GCTCTTCn'nnn_
Cuts at: 1065 2720 2930 1065
Size: 1655 210 2832
Fragments arranged by size:
2832 1655 210
SbfI CC_TGCA'GG
Cuts at: 1744 1744
Size: 4697
ScaI AGT'ACT
Cuts at: 4214 4214
Size: 4697
SfcI C'TryA_G
Cuts at: 33 247 1486 1740 2425 3418 3609 33
Size: 214 1239 254 685 993 191 1121
Fragments arranged by size:
1239 1121 993 685 254 214 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1312 1312
Size: 4697
SfoI GGC'GCC
Cuts at: 2129 2377 2129
Size: 248 4449
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4697
SgrAI Cr'CCGG_yG
Cuts at: 1574 1574
Size: 4697
SmaI CCC'GGG
Cuts at: 182 577 1165 1719 182
Size: 395 588 554 3160
Fragments arranged by size:
3160 588 554 395
SmlI C'TyrA_G
Cuts at: 1852 2767 3259 3521 1852
Size: 915 492 262 3028
Fragments arranged by size:
3028 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4697
SphI G_CATG'C
Cuts at: 1750 2250 1750
Size: 500 4197
StuI AGG'CCT
Cuts at: 1366 1366
Size: 4697
StyI C'CwwG_G
Cuts at: 107 832 1020 1831 2185 107
Size: 725 188 811 354 2619
Fragments arranged by size:
2619 811 725 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 451 1102 1114 1384 2280 2413 2872 3081 451
Size: 651 12 270 896 133 459 209 2067
Fragments arranged by size:
2067 896 651 459 270 209 133 12
TatI w'GTAC_w
Cuts at: 4212 4212
Size: 4697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4214 4246 4214
Size: 32 4665
Tth111I GACn'n_nGTC
Cuts at: 2494 2494
Size: 4697
XbaI T'CTAG_A
Cuts at: 1728 1728
Size: 4697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1194 2192 1194
Size: 998 3699
XmaI C'CCGG_G
Cuts at: 180 575 1163 1717 180
Size: 395 588 554 3160
Fragments arranged by size:
3160 588 554 395
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI ApaI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI