Restriction map for
pF1KB0491
(Circular) (Six-base) MAPSORT of: pF1KB0491.seq Check: 554 from: 1 to: 4892
pF1KB0491 4892 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 655 655
Size: 4892
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 1886 4732 1886
Size: 2846 2046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 2717 3149 3896 4164 999
Size: 1718 432 747 268 1727
Fragments arranged by size:
1727 1718 747 432 268
AfeI AGC'GCT
Cuts at: 1410 1410
Size: 4892
AflIII A'CryG_T
Cuts at: 2244 3348 4325 2244
Size: 1104 977 2811
Fragments arranged by size:
2811 1104 977
AgeI A'CCGG_T
Cuts at: 3280 3280
Size: 4892
AhdI GACnn_n'nnGTC
Cuts at: 444 444
Size: 4892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1593 1593
Size: 4892
AlwNI CAG_nnn'CTG
Cuts at: 327 498 1281 2197 3764 327
Size: 171 783 916 1567 1455
Fragments arranged by size:
1567 1455 916 783 171
ApaI G_GGCC'C
Cuts at: 885 885
Size: 4892
ApaLI G'TGCA_C
Cuts at: 3662 3662
Size: 4892
ApoI r'AATT_y
Cuts at: 1896 4245 4501 1896
Size: 2349 256 2287
Fragments arranged by size:
2349 2287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 181 190 1336 1473 1678 1717 1827 1912 181
Size: 9 1146 137 205 39 110 85 3161
Fragments arranged by size:
3161 1146 205 137 110 85 39 9
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 1387 1908 2322 2570 2605 1387
Size: 521 414 248 35 3674
Fragments arranged by size:
3674 521 414 248 35
BanII G_rGCy'C
Cuts at: 464 885 985 1106 1577 1718 1753 1906 2936
Size: 421 100 121 471 141 35 153 1030
Cuts at: 2936 464
Size: 2420
Fragments arranged by size:
2420 1030 471 421 153 141 121 100
35
BbeI G_GCGC'C
Cuts at: 1391 2326 2574 1391
Size: 935 248 3709
Fragments arranged by size:
3709 935 248
BbvCI CC'TCA_GC
Cuts at: 1792 1792
Size: 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 379 413 379
Size: 34 4858
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3551 4892 2790
Size: 761 1341 2790
Fragments arranged by size:
2790 1341 761
BclI T'GATC_A
Cuts at: 2412 2412
Size: 4892
BfrBI ATG'CAT
Cuts at: 1776 1776
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 931 943 1174 1198 931
Size: 12 231 24 4625
Fragments arranged by size:
4625 231 24 12
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 382 1834 2004 382
Size: 1452 170 3270
Fragments arranged by size:
3270 1452 170
Bme1580I G_kGCm'C
Cuts at: 885 2517 2610 3666 885
Size: 1632 93 1056 2111
Fragments arranged by size:
2111 1632 1056 93
BmgBI CAC'GTC
Cuts at: 867 995 1025 867
Size: 128 30 4734
Fragments arranged by size:
4734 128 30
BmrI ACTGGGnnnn_n'
Cuts at: 2104 2185 2520 4664 2104
Size: 81 335 2144 2332
Fragments arranged by size:
2332 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 222 564 1767 3259 222
Size: 342 1203 1492 1855
Fragments arranged by size:
1855 1492 1203 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 277 349 688 993 1207 244
Size: 33 72 339 305 214 3929
Fragments arranged by size:
3929 339 305 214 72 33
Bpu10I CC'TnA_GC
Cuts at: 1369 1603 1712 1792 1369
Size: 234 109 80 4469
Fragments arranged by size:
4469 234 109 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 2947 3439 3737 2068
Size: 879 492 298 3223
Fragments arranged by size:
3223 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 824 1234 4596 187
Size: 637 410 3362 483
Fragments arranged by size:
3362 637 483 410
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 2406 4443 6
Size: 2400 2037 455
Fragments arranged by size:
2400 2037 455
BsaHI Gr'CG_yC
Cuts at: 1362 1388 2323 2571 3273 4769 1362
Size: 26 935 248 702 1496 1485
Fragments arranged by size:
1496 1485 935 702 248 26
BsaWI w'CCGG_w
Cuts at: 2081 2602 3280 3554 3701 4112 2081
Size: 521 678 274 147 411 2861
Fragments arranged by size:
2861 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1519 1537 1549 1567 1519
Size: 18 12 18 4844
Fragments arranged by size:
4844 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 382 832 925 928 931 934 1045 379
Size: 3 450 93 3 3 3 111 4226
Fragments arranged by size:
4226 450 111 93 3 3 3 3
BseYI C'CCAG_C
Cuts at: 491 970 1271 1651 1799 1862 2317 3075 3652
Size: 479 301 380 148 63 455 758 577
Cuts at: 3652 4148 491
Size: 496 1235
Fragments arranged by size:
1235 758 577 496 479 455 380 301
148 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 559 1705 525
Size: 34 1146 3712
Fragments arranged by size:
3712 1146 34
BsiEI CG_ry'CG
Cuts at: 104 908 1113 1169 1238 1419 1494 1620 2434
Size: 804 205 56 69 181 75 126 814
Cuts at: 2434 2480 3688 104
Size: 46 1208 1308
Fragments arranged by size:
1308 1208 814 804 205 181 126 75
69 56 46
BsiHKAI G_wGCw'C
Cuts at: 464 1043 1106 1753 1906 2684 2874 3666 464
Size: 579 63 647 153 778 190 792 1690
Fragments arranged by size:
1690 792 778 647 579 190 153 63
Bsp1286I G_dGCh'C
Cuts at: 464 885 985 1043 1106 1577 1718 1753 1906
Size: 421 100 58 63 471 141 35 153
Cuts at: 1906 2517 2610 2684 2874 2936 3666 464
Size: 611 93 74 190 62 730 1690
Fragments arranged by size:
1690 730 611 471 421 190 153 141
100 93 74 63 62 58 35
BspEI T'CCGG_A
Cuts at: 4112 4112
Size: 4892
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 655 1942 2156 2458 2839 655
Size: 1287 214 302 381 2708
Fragments arranged by size:
2708 1287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 612 822 1506 1622 3184 3238 4722 4887
Size: 562 210 684 116 1562 54 1484 165
Cuts at: 4887 50
Size: 55
Fragments arranged by size:
1562 1484 684 562 210 165 116 55
54
BsrDI GCAATG_nn'
Cuts at: 653 2804 653
Size: 2151 2741
BsrFI r'CCGG_y
Cuts at: 833 1414 2890 3071 3280 833
Size: 581 1476 181 209 2445
Fragments arranged by size:
2445 1476 581 209 181
BssHII G'CGCG_C
Cuts at: 122 240 242 122
Size: 118 2 4772
Fragments arranged by size:
4772 118 2
BssSI C'ACGA_G
Cuts at: 388 3163 3521 388
Size: 2775 358 1759
Fragments arranged by size:
2775 1759 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstEII G'GTnAC_C
Cuts at: 429 429
Size: 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 666 1298 666
Size: 632 4260
BstYI r'GATC_y
Cuts at: 1 1917 2384 2407 2742 2988 4000 4086 4098
Size: 1916 467 23 335 246 1012 86 12
Cuts at: 4098 4444 1
Size: 346 449
Fragments arranged by size:
1916 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 760 1163 1169 1754 1813 107
Size: 653 403 6 585 59 3186
Fragments arranged by size:
3186 653 585 403 59 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2842 3023 3186 2842
Size: 181 163 4548
Fragments arranged by size:
4548 181 163
BtsI GCAGTG_nn'
Cuts at: 304 508 798 1633 2135 304
Size: 204 290 835 502 3061
Fragments arranged by size:
3061 835 502 290 204
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DraIII CAC_nnn'GTG
Cuts at: 534 534
Size: 4892
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3456 2598
Size: 858 4034
EaeI y'GGCC_r
Cuts at: 905 1166 1235 1416 1491 1617 2477 2651 3042
Size: 261 69 181 75 126 860 174 391
Cuts at: 3042 3069 4414 905
Size: 27 1345 1383
Fragments arranged by size:
1383 1345 860 391 261 181 174 126
75 69 27
EagI C'GGCC_G
Cuts at: 905 1166 1235 1416 1491 1617 2477 905
Size: 261 69 181 75 126 860 3320
Fragments arranged by size:
3320 860 261 181 126 75 69
EarI CTCTTCn'nnn_
Cuts at: 1111 2915 3125 1111
Size: 1804 210 2878
Fragments arranged by size:
2878 1804 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 705 1230 3100 3410 3556 4702 705
Size: 525 1870 310 146 1146 895
Fragments arranged by size:
1870 1146 895 525 310 146
EcoICRI GAG'CTC
Cuts at: 462 1104 1751 1904 462
Size: 642 647 153 3450
Fragments arranged by size:
3450 647 642 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 277 349 688 993 999 1207 2717 3149
Size: 33 72 339 305 6 208 1510 432
Cuts at: 3149 3896 4164 244
Size: 747 268 972
Fragments arranged by size:
1510 972 747 432 339 305 268 208
72 33 6
EcoNI CCTnn'n_nnAGG
Cuts at: 519 1555 1855 519
Size: 1036 300 3556
Fragments arranged by size:
3556 1036 300
EcoO109I rG'GnC_Cy
Cuts at: 1267 1278 2031 1267
Size: 11 753 4128
Fragments arranged by size:
4128 753 11
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 151 280 2673 151
Size: 129 2393 2370
Fragments arranged by size:
2393 2370 129
HaeII r_GCGC'y
Cuts at: 1022 1391 1412 2326 2574 3596 4575 1022
Size: 369 21 914 248 1022 979 1339
Fragments arranged by size:
1339 1022 979 914 369 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 1519 1537 1551 1569 1596 1628 2734
Size: 32 1057 18 14 18 27 32 1106
Cuts at: 2734 2764 2766 2796 430
Size: 30 2 30 2526
Fragments arranged by size:
2526 1106 1057 32 32 30 30 27
18 18 14 2
HincII GTy'rAC
Cuts at: 1931 1931
Size: 4892
KasI G'GCGC_C
Cuts at: 1387 2322 2570 1387
Size: 935 248 3709
Fragments arranged by size:
3709 935 248
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1007 3563 3747 1007
Size: 2556 184 2152
Fragments arranged by size:
2556 2152 184
MscI TGG'CCA
Cuts at: 2653 2653
Size: 4892
MslI CAynn'nnrTG
Cuts at: 2881 3008 2881
Size: 127 4765
MspA1I CmG'CkG
Cuts at: 495 762 1171 1185 1689 1815 2003 2317 2677
Size: 267 409 14 504 126 188 314 360
Cuts at: 2677 3690 3935 495
Size: 1013 245 1452
Fragments arranged by size:
1452 1013 504 409 360 314 267 245
188 126 14
NaeI GCC'GGC
Cuts at: 1416 3073 1416
Size: 1657 3235
NarI GG'CG_CC
Cuts at: 1388 2323 2571 1388
Size: 935 248 3709
Fragments arranged by size:
3709 935 248
NcoI C'CATG_G
Cuts at: 107 1754 107
Size: 1647 3245
NgoMIV G'CCGG_C
Cuts at: 1414 3071 1414
Size: 1657 3235
NsiI A_TGCA'T
Cuts at: 1778 1778
Size: 4892
NspI r_CATG'y
Cuts at: 1945 2445 3352 1945
Size: 500 907 3485
Fragments arranged by size:
3485 907 500
PasI CC'CwG_GG
Cuts at: 1557 1557
Size: 4892
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4892
PflMI CCAn_nnn'nTGG
Cuts at: 222 327 222
Size: 105 4787
PfoI T'CCnGG_A
Cuts at: 557 557
Size: 4892
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 184 216 4031 4063 184
Size: 32 3815 32 1013
Fragments arranged by size:
3815 1013 32 32
PpuMI rG'GwC_Cy
Cuts at: 1267 1267
Size: 4892
PspOMI G'GGCC_C
Cuts at: 881 881
Size: 4892
PstI C_TGCA'G
Cuts at: 131 311 503 598 793 1435 1939 2624 131
Size: 180 192 95 195 642 504 685 2399
Fragments arranged by size:
2399 685 642 504 195 192 180 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 495 1689 2317 2677 495
Size: 1194 628 360 2710
Fragments arranged by size:
2710 1194 628 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 464 1106 1753 1906 464
Size: 642 647 153 3450
Fragments arranged by size:
3450 647 642 153
SacII CC_GC'GG
Cuts at: 763 1172 1816 763
Size: 409 644 3839
Fragments arranged by size:
3839 644 409
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SanDI GG'GwC_CC
Cuts at: 1267 1267
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 1111 2915 3125 1111
Size: 1804 210 2878
Fragments arranged by size:
2878 1804 210
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SfcI C'TryA_G
Cuts at: 33 127 307 465 499 594 789 1431 1935
Size: 94 180 158 34 95 195 642 504
Cuts at: 1935 2620 3613 3804 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 504 195 191 180
158 95 94 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 1198 1198
Size: 4892
SfoI GGC'GCC
Cuts at: 1389 2324 2572 1389
Size: 935 248 3709
Fragments arranged by size:
3709 935 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1338 1475 1914 1338
Size: 137 439 4316
Fragments arranged by size:
4316 439 137
SmlI C'TyrA_G
Cuts at: 181 190 2047 2962 3454 3716 181
Size: 9 1857 915 492 262 1357
Fragments arranged by size:
1857 1357 915 492 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4392
SrfI GCCC'GGGC
Cuts at: 1475 1475
Size: 4892
StuI AGG'CCT
Cuts at: 964 1762 1807 964
Size: 798 45 4049
Fragments arranged by size:
4049 798 45
StyI C'CwwG_G
Cuts at: 107 425 659 1157 1739 1754 1856 2026 2380
Size: 318 234 498 582 15 102 170 354
Cuts at: 2380 107
Size: 2619
Fragments arranged by size:
2619 582 498 354 318 234 170 102
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 211 877 1665 2475 2608 3067 3276 211
Size: 666 788 810 133 459 209 1827
Fragments arranged by size:
1827 810 788 666 459 209 133
TatI w'GTAC_w
Cuts at: 4407 4407
Size: 4892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 4860
Tth111I GACn'n_nGTC
Cuts at: 637 1065 1221 2689 637
Size: 428 156 1468 2840
Fragments arranged by size:
2840 1468 428 156
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1170 1737 2387 1170
Size: 567 650 3675
Fragments arranged by size:
3675 650 567
XhoI C'TCGA_G
Cuts at: 181 190 181
Size: 9 4883
XmaI C'CCGG_G
Cuts at: 1336 1473 1912 1336
Size: 137 439 4316
Fragments arranged by size:
4316 439 137
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BbsI
BmtI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PmlI PshAI PsiI PspXI
PsrI SexAI SgrAI SnaBI SspI SwaI XmnI ZraI