(Circular) (Six-base) MAPSORT of: pF1KB0494.seq Check: 622 from: 1 to: 6230 pF1KB0494 6230 bp With 182 enzymes: SgfI * October 7, 2009 14:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2165 2165 Size: 6230 AatII G_ACGT'C Cuts at: 509 1094 509 Size: 585 5645 AccI GT'mk_AC Cuts at: 1475 3268 1475 Size: 1793 4437 Acc65I G'GTAC_C Cuts at: 3246 3246 Size: 6230 AclI AA'CG_TT Cuts at: 6070 6070 Size: 6230 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 696 2020 2656 2800 4055 4487 5234 5502 696 Size: 1324 636 144 1255 432 747 268 1424 Fragments arranged by size: 1424 1324 1255 747 636 432 268 144 AflIII A'CryG_T Cuts at: 845 2993 3582 4686 5663 845 Size: 2148 589 1104 977 1412 Fragments arranged by size: 2148 1412 1104 977 589 AgeI A'CCGG_T Cuts at: 4618 4618 Size: 6230 AleI CACnn'nnGTG Cuts at: 1972 1972 Size: 6230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2422 2422 Size: 6230 AlwNI CAG_nnn'CTG Cuts at: 1029 1081 1330 1419 1444 1581 3535 5102 1029 Size: 52 249 89 25 137 1954 1567 2157 Fragments arranged by size: 2157 1954 1567 249 137 89 52 25 ApaI G_GGCC'C Cuts at: 334 2187 334 Size: 1853 4377 ApaLI G'TGCA_C Cuts at: 2152 5000 2152 Size: 2848 3382 ApoI r'AATT_y Cuts at: 1004 3234 5583 5839 1004 Size: 2230 2349 256 1395 Fragments arranged by size: 2349 2230 1395 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6230 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6230 AvaI C'yCGr_G Cuts at: 813 2797 2808 3035 3250 813 Size: 1984 11 227 215 3793 Fragments arranged by size: 3793 1984 227 215 11 BamHI G'GATC_C Cuts at: 2820 3255 2820 Size: 435 5795 BanI G'GyrC_C Cuts at: 313 967 1222 1468 1789 2167 2847 3015 3246 Size: 654 255 246 321 378 680 168 231 Cuts at: 3246 3660 3908 3943 313 Size: 414 248 35 2600 Fragments arranged by size: 2600 680 654 414 378 321 255 248 246 231 168 35 BanII G_rGCy'C Cuts at: 334 756 1551 1777 1891 2062 2187 2558 2798 Size: 422 795 226 114 171 125 371 240 Cuts at: 2798 3244 4274 334 Size: 446 1030 2290 Fragments arranged by size: 2290 1030 795 446 422 371 240 226 171 125 114 BbeI G_GCGC'C Cuts at: 317 2171 2851 3664 3912 317 Size: 1854 680 813 248 2635 Fragments arranged by size: 2635 1854 813 680 248 BbsI GAAGACnn'nnnn_ Cuts at: 577 2581 577 Size: 2004 4226 BbvCI CC'TCA_GC Cuts at: 1396 2596 1396 Size: 1200 5030 BciVI GTATCCnnnnn_n' Cuts at: 1486 4128 4889 6230 1486 Size: 2642 761 1341 1486 Fragments arranged by size: 2642 1486 1341 761 BclI T'GATC_A Cuts at: 3750 3750 Size: 6230 BglI GCCn_nnn'nGGC Cuts at: 245 2811 245 Size: 2566 3664 BglII A'GATC_T Cuts at: 2780 3745 5782 2780 Size: 965 2037 3228 Fragments arranged by size: 3228 2037 965 BlpI GC'TnA_GC Cuts at: 599 1550 1885 1900 3025 3342 599 Size: 951 335 15 1125 317 3487 Fragments arranged by size: 3487 1125 951 335 317 15 Bme1580I G_kGCm'C Cuts at: 334 370 397 972 1227 1688 2030 2156 2187 Size: 36 27 575 255 461 342 126 31 Cuts at: 2187 3020 3855 3948 5004 334 Size: 833 835 93 1056 1560 Fragments arranged by size: 1560 1056 835 833 575 461 342 255 126 93 36 31 27 BmgBI CAC'GTC Cuts at: 1405 2418 1405 Size: 1013 5217 BmrI ACTGGGnnnn_n' Cuts at: 208 487 912 1343 1691 2724 3442 3523 3858 Size: 279 425 431 348 1033 718 81 335 Cuts at: 3858 6002 208 Size: 2144 436 Fragments arranged by size: 2144 1033 718 436 431 425 348 335 279 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2823 4597 2823 Size: 1774 4456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 169 173 393 519 779 858 2113 169 Size: 4 220 126 260 79 1255 4286 Fragments arranged by size: 4286 1255 260 220 126 79 4 Bpu10I CC'TnA_GC Cuts at: 652 1396 2188 2596 3047 652 Size: 744 792 408 451 3835 Fragments arranged by size: 3835 792 744 451 408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2689 3406 4285 4777 5075 2689 Size: 717 879 492 298 3844 Fragments arranged by size: 3844 879 717 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 418 784 2236 5934 418 Size: 366 1452 3698 714 Fragments arranged by size: 3698 1452 714 366 BsaAI yAC'GTr Cuts at: 666 846 3583 666 Size: 180 2737 3313 Fragments arranged by size: 3313 2737 180 BsaBI GATnn'nnATC Cuts at: 6 2299 3744 5781 6 Size: 2293 1445 2037 455 Fragments arranged by size: 2293 2037 1445 455 BsaHI Gr'CG_yC Cuts at: 314 484 506 574 1057 1091 2168 2848 3661 Size: 170 22 68 483 34 1077 680 813 Cuts at: 3661 3909 4611 6107 314 Size: 248 702 1496 437 Fragments arranged by size: 1496 1077 813 702 680 483 437 248 170 68 34 22 BsaWI w'CCGG_w Cuts at: 3419 3940 4618 4892 5039 5450 3419 Size: 521 678 274 147 411 4199 Fragments arranged by size: 4199 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 728 758 835 865 2335 2365 2983 3013 3050 Size: 30 77 30 1470 30 618 30 37 Cuts at: 3050 3080 728 Size: 30 3878 Fragments arranged by size: 3878 1470 618 77 37 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 358 574 3094 358 Size: 216 2520 3494 Fragments arranged by size: 3494 2520 216 BseYI C'CCAG_C Cuts at: 334 683 1218 1228 1770 2030 2265 2338 2525 Size: 349 535 10 542 260 235 73 187 Cuts at: 2525 2621 2731 2935 3120 3655 4413 4990 5486 Size: 96 110 204 185 535 758 577 496 Cuts at: 5486 334 Size: 1078 Fragments arranged by size: 1078 758 577 542 535 535 496 349 260 235 204 187 185 110 96 73 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 238 1996 2884 238 Size: 1758 888 3584 Fragments arranged by size: 3584 1758 888 BsiEI CG_ry'CG Cuts at: 104 491 615 1302 3772 3818 5026 104 Size: 387 124 687 2470 46 1208 1308 Fragments arranged by size: 2470 1308 1208 687 387 124 46 BsiHKAI G_wGCw'C Cuts at: 1551 1901 2156 2546 2558 3244 4022 4212 5004 Size: 350 255 390 12 686 778 190 792 Cuts at: 5004 1551 Size: 2777 Fragments arranged by size: 2777 792 778 686 390 350 255 190 12 BsmI GAATG_Cn' Cuts at: 1071 1071 Size: 6230 BsmBI CGTCTCn'nnnn_ Cuts at: 340 340 Size: 6230 Bsp1286I G_dGCh'C Cuts at: 334 370 397 756 972 1227 1551 1688 1777 Size: 36 27 359 216 255 324 137 89 Cuts at: 1777 1891 1901 2030 2062 2156 2187 2546 2558 Size: 114 10 129 32 94 31 359 12 Cuts at: 2558 2798 3020 3244 3855 3948 4022 4212 4274 Size: 240 222 224 611 93 74 190 62 Cuts at: 4274 5004 334 Size: 730 1560 Fragments arranged by size: 1560 730 611 359 359 324 255 240 224 222 216 190 137 129 114 94 93 89 74 62 36 32 31 27 12 10 BspEI T'CCGG_A Cuts at: 5450 5450 Size: 6230 BspHI T'CATG_A Cuts at: 5406 5406 Size: 6230 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2165 2642 2882 3280 3494 3796 4177 2165 Size: 477 240 398 214 302 381 4218 Fragments arranged by size: 4218 477 398 381 302 240 214 BsrBI CCG'CTC Cuts at: 50 611 997 2067 3024 4522 4576 6060 6225 Size: 561 386 1070 957 1498 54 1484 165 Cuts at: 6225 50 Size: 55 Fragments arranged by size: 1498 1484 1070 957 561 386 165 55 54 BsrDI GCAATG_nn' Cuts at: 1319 2199 4142 1319 Size: 880 1943 3407 Fragments arranged by size: 3407 1943 880 BsrFI r'CCGG_y Cuts at: 1733 1754 2421 3188 4228 4409 4618 1733 Size: 21 667 767 1040 181 209 3345 Fragments arranged by size: 3345 1040 767 667 209 181 21 BsrGI T'GTAC_A Cuts at: 1541 1541 Size: 6230 BssHII G'CGCG_C Cuts at: 2887 2887 Size: 6230 BssSI C'ACGA_G Cuts at: 4501 4859 4501 Size: 358 5872 BstAPI GCAn_nnn'nTGC Cuts at: 1038 1116 1330 3607 1038 Size: 78 214 2277 3661 Fragments arranged by size: 3661 2277 214 78 BstBI TT'CG_AA Cuts at: 4591 4591 Size: 6230 BstXI CCAn_nnnn'nTGG Cuts at: 309 1533 2566 3101 309 Size: 1224 1033 535 3438 Fragments arranged by size: 3438 1224 1033 535 BstYI r'GATC_y Cuts at: 1 939 1806 2780 2820 3255 3722 3745 4080 Size: 938 867 974 40 435 467 23 335 Cuts at: 4080 4326 5338 5424 5436 5782 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 974 938 867 467 449 435 346 335 246 86 40 23 12 BtgI C'CryG_G Cuts at: 107 288 319 719 920 1844 1925 2903 107 Size: 181 31 400 201 924 81 978 3434 Fragments arranged by size: 3434 978 924 400 201 181 81 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1263 2320 4180 4361 4524 1263 Size: 1057 1860 181 163 2969 Fragments arranged by size: 2969 1860 1057 181 163 BtsI GCAGTG_nn' Cuts at: 127 1331 2042 2872 3473 127 Size: 1204 711 830 601 2884 Fragments arranged by size: 2884 1204 830 711 601 ClaI AT'CG_AT Cuts at: 1481 1481 Size: 6230 DraI TTT'AAA Cuts at: 3229 3229 Size: 6230 DrdI GACnn_nn'nnGTC Cuts at: 2422 3936 4794 2422 Size: 1514 858 3858 Fragments arranged by size: 3858 1514 858 EaeI y'GGCC_r Cuts at: 110 299 387 612 917 1166 1353 2147 3155 Size: 189 88 225 305 249 187 794 1008 Cuts at: 3155 3190 3815 3989 4380 4407 5752 110 Size: 35 625 174 391 27 1345 588 Fragments arranged by size: 1345 1008 794 625 588 391 305 249 225 189 187 174 88 35 27 EagI C'GGCC_G Cuts at: 612 3815 612 Size: 3203 3027 EarI CTCTTCn'nnn_ Cuts at: 1606 2327 3209 4253 4463 1606 Size: 721 882 1044 210 3373 Fragments arranged by size: 3373 1044 882 721 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1829 4438 4748 4894 6040 1829 Size: 2609 310 146 1146 2019 Fragments arranged by size: 2609 2019 1146 310 146 EcoICRI GAG'CTC Cuts at: 1549 2556 3242 1549 Size: 1007 686 4537 Fragments arranged by size: 4537 1007 686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 169 173 393 519 696 779 858 2020 2113 Size: 4 220 126 177 83 79 1162 93 Cuts at: 2113 2656 2800 4055 4487 5234 5502 169 Size: 543 144 1255 432 747 268 897 Fragments arranged by size: 1255 1162 897 747 543 432 268 220 177 144 126 93 83 79 4 EcoNI CCTnn'n_nnAGG Cuts at: 787 2161 787 Size: 1374 4856 EcoO109I rG'GnC_Cy Cuts at: 172 238 331 1207 1345 1416 2129 2184 2530 Size: 66 93 876 138 71 713 55 346 Cuts at: 2530 2667 3369 172 Size: 137 702 3033 Fragments arranged by size: 3033 876 713 702 346 138 137 93 71 66 55 EcoRI G'AATT_C Cuts at: 3234 3234 Size: 6230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3189 4249 3189 Size: 1060 5170 FspI TGC'GCA Cuts at: 4011 4011 Size: 6230 HaeII r_GCGC'y Cuts at: 317 1130 2171 2851 3664 3912 4934 5913 317 Size: 813 1041 680 813 248 1022 979 634 Fragments arranged by size: 1041 1022 979 813 813 680 634 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 287 319 796 828 1017 1049 1098 1130 1389 Size: 32 477 32 189 32 49 32 259 Cuts at: 1389 1421 2335 2367 4072 4102 4104 4134 287 Size: 32 914 32 1705 30 2 30 2383 Fragments arranged by size: 2383 1705 914 477 259 189 49 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1095 2697 3269 1095 Size: 1602 572 4056 Fragments arranged by size: 4056 1602 572 KasI G'GCGC_C Cuts at: 313 2167 2847 3660 3908 313 Size: 1854 680 813 248 2635 Fragments arranged by size: 2635 1854 813 680 248 KpnI G_GTAC'C Cuts at: 3250 3250 Size: 6230 MluI A'CGCG_T Cuts at: 5663 5663 Size: 6230 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1653 2654 4901 5085 1653 Size: 1001 2247 184 2798 Fragments arranged by size: 2798 2247 1001 184 MscI TGG'CCA Cuts at: 112 301 3157 3991 112 Size: 189 2856 834 2351 Fragments arranged by size: 2856 2351 834 189 MslI CAynn'nnrTG Cuts at: 307 664 1324 1663 1843 1972 2549 2564 4219 Size: 357 660 339 180 129 577 15 1655 Cuts at: 4219 4346 307 Size: 127 2191 Fragments arranged by size: 2191 1655 660 577 357 339 180 129 127 15 MspA1I CmG'CkG Cuts at: 321 497 1728 2361 2525 2898 3063 3341 3655 Size: 176 1231 633 164 373 165 278 314 Cuts at: 3655 4015 5028 5273 321 Size: 360 1013 245 1278 Fragments arranged by size: 1278 1231 1013 633 373 360 314 278 245 176 165 164 NaeI GCC'GGC Cuts at: 1756 4411 1756 Size: 2655 3575 NarI GG'CG_CC Cuts at: 314 2168 2848 3661 3909 314 Size: 1854 680 813 248 2635 Fragments arranged by size: 2635 1854 813 680 248 NcoI C'CATG_G Cuts at: 107 288 1844 2903 107 Size: 181 1556 1059 3434 Fragments arranged by size: 3434 1556 1059 181 NgoMIV G'CCGG_C Cuts at: 1754 4409 1754 Size: 2655 3575 NotI GC'GGCC_GC Cuts at: 612 612 Size: 6230 NruI TCG'CGA Cuts at: 2305 2305 Size: 6230 NspI r_CATG'y Cuts at: 2181 3283 3783 4690 2181 Size: 1102 500 907 3721 Fragments arranged by size: 3721 1102 907 500 PasI CC'CwG_GG Cuts at: 327 990 1287 2163 2572 2661 327 Size: 663 297 876 409 89 3896 Fragments arranged by size: 3896 876 663 409 297 89 PciI A'CATG_T Cuts at: 4686 4686 Size: 6230 PflMI CCAn_nnn'nTGG Cuts at: 477 1218 1368 1419 1581 477 Size: 741 150 51 162 5126 Fragments arranged by size: 5126 741 162 150 51 PfoI T'CCnGG_A Cuts at: 1418 1874 1997 2090 2610 2645 1418 Size: 456 123 93 520 35 5003 Fragments arranged by size: 5003 520 456 123 93 35 PmeI GTTT'AAAC Cuts at: 3229 3229 Size: 6230 PmlI CAC'GTG Cuts at: 846 846 Size: 6230 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5369 5401 5369 Size: 32 6198 PpuMI rG'GwC_Cy Cuts at: 172 1345 1416 2530 172 Size: 1173 71 1114 3872 Fragments arranged by size: 3872 1173 1114 71 PspOMI G'GGCC_C Cuts at: 330 2183 330 Size: 1853 4377 PspXI vC'TCGA_Gb Cuts at: 2808 2808 Size: 6230 PstI C_TGCA'G Cuts at: 1199 1727 2102 2366 2879 2975 3277 3962 1199 Size: 528 375 264 513 96 302 685 3467 Fragments arranged by size: 3467 685 528 513 375 302 264 96 PvuI CG_AT'CG Cuts at: 104 104 Size: 6230 PvuII CAG'CTG Cuts at: 497 1728 2361 2525 3063 3655 4015 497 Size: 1231 633 164 538 592 360 2712 Fragments arranged by size: 2712 1231 633 592 538 360 164 RsrII CG'GwC_CG Cuts at: 2424 4425 2424 Size: 2001 4229 SacI G_AGCT'C Cuts at: 1551 2558 3244 1551 Size: 1007 686 4537 Fragments arranged by size: 4537 1007 686 SacII CC_GC'GG Cuts at: 322 322 Size: 6230 SalI G'TCGA_C Cuts at: 3267 3267 Size: 6230 SanDI GG'GwC_CC Cuts at: 1345 2530 1345 Size: 1185 5045 SapI GCTCTTCn'nnn_ Cuts at: 1606 4253 4463 1606 Size: 2647 210 3373 Fragments arranged by size: 3373 2647 210 SbfI CC_TGCA'GG Cuts at: 2879 3277 2879 Size: 398 5832 ScaI AGT'ACT Cuts at: 628 889 5747 628 Size: 261 4858 1111 Fragments arranged by size: 4858 1111 261 SexAI A'CCwGG_T Cuts at: 1529 1529 Size: 6230 SfcI C'TryA_G Cuts at: 33 950 1195 1723 2098 2362 2875 2971 3273 Size: 917 245 528 375 264 513 96 302 Cuts at: 3273 3958 4951 5142 33 Size: 685 993 191 1121 Fragments arranged by size: 1121 993 917 685 528 513 375 302 264 245 191 96 SfoI GGC'GCC Cuts at: 315 2169 2849 3662 3910 315 Size: 1854 680 813 248 2635 Fragments arranged by size: 2635 1854 813 680 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6230 SmaI CCC'GGG Cuts at: 3252 3252 Size: 6230 SmlI C'TyrA_G Cuts at: 813 2704 2808 3385 4300 4792 5054 813 Size: 1891 104 577 915 492 262 1989 Fragments arranged by size: 1989 1891 915 577 492 262 104 SpeI A'CTAG_T Cuts at: 67 67 Size: 6230 SphI G_CATG'C Cuts at: 3283 3783 3283 Size: 500 5730 StuI AGG'CCT Cuts at: 1567 2952 1567 Size: 1385 4845 StyI C'CwwG_G Cuts at: 107 288 471 1844 2903 3074 3364 3718 107 Size: 181 183 1373 1059 171 290 354 2619 Fragments arranged by size: 2619 1373 1059 354 290 183 181 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 144 477 1172 1210 1984 3093 3103 3112 3813 Size: 333 695 38 774 1109 10 9 701 Cuts at: 3813 3946 4405 4614 144 Size: 133 459 209 1760 Fragments arranged by size: 1760 1109 774 701 695 459 333 209 133 38 10 9 TatI w'GTAC_w Cuts at: 626 887 1541 2854 5745 626 Size: 261 654 1313 2891 1111 Fragments arranged by size: 2891 1313 1111 654 261 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 257 289 728 760 835 867 1979 2011 2522 Size: 32 439 32 75 32 1112 32 511 Cuts at: 2522 2554 3050 3082 5747 5779 257 Size: 32 496 32 2665 32 708 Fragments arranged by size: 2665 1112 708 511 496 439 75 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4027 4027 Size: 6230 XbaI T'CTAG_A Cuts at: 3261 3261 Size: 6230 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1284 1669 1800 3150 3725 1284 Size: 385 131 1350 575 3789 Fragments arranged by size: 3789 1350 575 385 131 XhoI C'TCGA_G Cuts at: 813 2808 813 Size: 1995 4235 XmaI C'CCGG_G Cuts at: 3250 3250 Size: 6230 ZraI GAC'GTC Cuts at: 507 1092 507 Size: 585 5645 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AloI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NsiI PacI PshAI PsiI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI