Restriction map for pF1KB0494
 (Circular) (Six-base) MAPSORT of: pF1KB0494.seq  Check: 622  from: 1  to: 6230



pF1KB0494  6230 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2165    2165 
   Size:       6230
 
AatII G_ACGT'C

Cuts at:    509    1094     509 
   Size:        585    5645
 
AccI GT'mk_AC

Cuts at:   1475    3268    1475 
   Size:       1793    4437
 
Acc65I G'GTAC_C

Cuts at:   3246    3246 
   Size:       6230
 
AclI AA'CG_TT

Cuts at:   6070    6070 
   Size:       6230
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    696    2020    2656    2800    4055    4487    5234    5502     696 
   Size:       1324     636     144    1255     432     747     268    1424

  Fragments arranged by size: 

                1424    1324    1255     747     636     432     268     144
            
 
AflIII A'CryG_T

Cuts at:    845    2993    3582    4686    5663     845 
   Size:       2148     589    1104     977    1412

  Fragments arranged by size: 

                2148    1412    1104     977     589
 
AgeI A'CCGG_T

Cuts at:   4618    4618 
   Size:       6230
 
AleI CACnn'nnGTG

Cuts at:   1972    1972 
   Size:       6230
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2422    2422 
   Size:       6230
 
AlwNI CAG_nnn'CTG

Cuts at:   1029    1081    1330    1419    1444    1581    3535    5102    1029 
   Size:         52     249      89      25     137    1954    1567    2157

  Fragments arranged by size: 

                2157    1954    1567     249     137      89      52      25
            
 
ApaI G_GGCC'C

Cuts at:    334    2187     334 
   Size:       1853    4377
 
ApaLI G'TGCA_C

Cuts at:   2152    5000    2152 
   Size:       2848    3382
 
ApoI r'AATT_y

Cuts at:   1004    3234    5583    5839    1004 
   Size:       2230    2349     256    1395

  Fragments arranged by size: 

                2349    2230    1395     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6230
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6230
 
AvaI C'yCGr_G

Cuts at:    813    2797    2808    3035    3250     813 
   Size:       1984      11     227     215    3793

  Fragments arranged by size: 

                3793    1984     227     215      11
 
BamHI G'GATC_C

Cuts at:   2820    3255    2820 
   Size:        435    5795
 
BanI G'GyrC_C

Cuts at:    313     967    1222    1468    1789    2167    2847    3015    3246 
   Size:        654     255     246     321     378     680     168     231

Cuts at:   3246    3660    3908    3943     313 
   Size:        414     248      35    2600

  Fragments arranged by size: 

                2600     680     654     414     378     321     255     248
                 246     231     168      35
 
BanII G_rGCy'C

Cuts at:    334     756    1551    1777    1891    2062    2187    2558    2798 
   Size:        422     795     226     114     171     125     371     240

Cuts at:   2798    3244    4274     334 
   Size:        446    1030    2290

  Fragments arranged by size: 

                2290    1030     795     446     422     371     240     226
                 171     125     114
 
BbeI G_GCGC'C

Cuts at:    317    2171    2851    3664    3912     317 
   Size:       1854     680     813     248    2635

  Fragments arranged by size: 

                2635    1854     813     680     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    577    2581     577 
   Size:       2004    4226
 
BbvCI CC'TCA_GC

Cuts at:   1396    2596    1396 
   Size:       1200    5030
 
BciVI GTATCCnnnnn_n'

Cuts at:   1486    4128    4889    6230    1486 
   Size:       2642     761    1341    1486

  Fragments arranged by size: 

                2642    1486    1341     761
 
BclI T'GATC_A

Cuts at:   3750    3750 
   Size:       6230
 
BglI GCCn_nnn'nGGC

Cuts at:    245    2811     245 
   Size:       2566    3664
 
BglII A'GATC_T

Cuts at:   2780    3745    5782    2780 
   Size:        965    2037    3228

  Fragments arranged by size: 

                3228    2037     965
 
BlpI GC'TnA_GC

Cuts at:    599    1550    1885    1900    3025    3342     599 
   Size:        951     335      15    1125     317    3487

  Fragments arranged by size: 

                3487    1125     951     335     317      15
 
Bme1580I G_kGCm'C

Cuts at:    334     370     397     972    1227    1688    2030    2156    2187 
   Size:         36      27     575     255     461     342     126      31

Cuts at:   2187    3020    3855    3948    5004     334 
   Size:        833     835      93    1056    1560

  Fragments arranged by size: 

                1560    1056     835     833     575     461     342     255
                 126      93      36      31      27
 
BmgBI CAC'GTC

Cuts at:   1405    2418    1405 
   Size:       1013    5217
 
BmrI ACTGGGnnnn_n'

Cuts at:    208     487     912    1343    1691    2724    3442    3523    3858 
   Size:        279     425     431     348    1033     718      81     335

Cuts at:   3858    6002     208 
   Size:       2144     436

  Fragments arranged by size: 

                2144    1033     718     436     431     425     348     335
                 279      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2823    4597    2823 
   Size:       1774    4456
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    169     173     393     519     779     858    2113     169 
   Size:          4     220     126     260      79    1255    4286

  Fragments arranged by size: 

                4286    1255     260     220     126      79       4
 
Bpu10I CC'TnA_GC

Cuts at:    652    1396    2188    2596    3047     652 
   Size:        744     792     408     451    3835

  Fragments arranged by size: 

                3835     792     744     451     408
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2689    3406    4285    4777    5075    2689 
   Size:        717     879     492     298    3844

  Fragments arranged by size: 

                3844     879     717     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    418     784    2236    5934     418 
   Size:        366    1452    3698     714

  Fragments arranged by size: 

                3698    1452     714     366
 
BsaAI yAC'GTr

Cuts at:    666     846    3583     666 
   Size:        180    2737    3313

  Fragments arranged by size: 

                3313    2737     180
 
BsaBI GATnn'nnATC

Cuts at:      6    2299    3744    5781       6 
   Size:       2293    1445    2037     455

  Fragments arranged by size: 

                2293    2037    1445     455
 
BsaHI Gr'CG_yC

Cuts at:    314     484     506     574    1057    1091    2168    2848    3661 
   Size:        170      22      68     483      34    1077     680     813

Cuts at:   3661    3909    4611    6107     314 
   Size:        248     702    1496     437

  Fragments arranged by size: 

                1496    1077     813     702     680     483     437     248
                 170      68      34      22
 
BsaWI w'CCGG_w

Cuts at:   3419    3940    4618    4892    5039    5450    3419 
   Size:        521     678     274     147     411    4199

  Fragments arranged by size: 

                4199     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    728     758     835     865    2335    2365    2983    3013    3050 
   Size:         30      77      30    1470      30     618      30      37

Cuts at:   3050    3080     728 
   Size:         30    3878

  Fragments arranged by size: 

                3878    1470     618      77      37      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    358     574    3094     358 
   Size:        216    2520    3494

  Fragments arranged by size: 

                3494    2520     216
 
BseYI C'CCAG_C

Cuts at:    334     683    1218    1228    1770    2030    2265    2338    2525 
   Size:        349     535      10     542     260     235      73     187

Cuts at:   2525    2621    2731    2935    3120    3655    4413    4990    5486 
   Size:         96     110     204     185     535     758     577     496

Cuts at:   5486     334 
   Size:       1078

  Fragments arranged by size: 

                1078     758     577     542     535     535     496     349
                 260     235     204     187     185     110      96      73
                  10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    238    1996    2884     238 
   Size:       1758     888    3584

  Fragments arranged by size: 

                3584    1758     888
 
BsiEI CG_ry'CG

Cuts at:    104     491     615    1302    3772    3818    5026     104 
   Size:        387     124     687    2470      46    1208    1308

  Fragments arranged by size: 

                2470    1308    1208     687     387     124      46
 
BsiHKAI G_wGCw'C

Cuts at:   1551    1901    2156    2546    2558    3244    4022    4212    5004 
   Size:        350     255     390      12     686     778     190     792

Cuts at:   5004    1551 
   Size:       2777

  Fragments arranged by size: 

                2777     792     778     686     390     350     255     190
                  12
 
BsmI GAATG_Cn'

Cuts at:   1071    1071 
   Size:       6230
 
BsmBI CGTCTCn'nnnn_

Cuts at:    340     340 
   Size:       6230
 
Bsp1286I G_dGCh'C

Cuts at:    334     370     397     756     972    1227    1551    1688    1777 
   Size:         36      27     359     216     255     324     137      89

Cuts at:   1777    1891    1901    2030    2062    2156    2187    2546    2558 
   Size:        114      10     129      32      94      31     359      12

Cuts at:   2558    2798    3020    3244    3855    3948    4022    4212    4274 
   Size:        240     222     224     611      93      74     190      62

Cuts at:   4274    5004     334 
   Size:        730    1560

  Fragments arranged by size: 

                1560     730     611     359     359     324     255     240
                 224     222     216     190     137     129     114      94
                  93      89      74      62      36      32      31      27
                  12      10
 
BspEI T'CCGG_A

Cuts at:   5450    5450 
   Size:       6230
 
BspHI T'CATG_A

Cuts at:   5406    5406 
   Size:       6230
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2165    2642    2882    3280    3494    3796    4177    2165 
   Size:        477     240     398     214     302     381    4218

  Fragments arranged by size: 

                4218     477     398     381     302     240     214
 
BsrBI CCG'CTC

Cuts at:     50     611     997    2067    3024    4522    4576    6060    6225 
   Size:        561     386    1070     957    1498      54    1484     165

Cuts at:   6225      50 
   Size:         55

  Fragments arranged by size: 

                1498    1484    1070     957     561     386     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   1319    2199    4142    1319 
   Size:        880    1943    3407

  Fragments arranged by size: 

                3407    1943     880
 
BsrFI r'CCGG_y

Cuts at:   1733    1754    2421    3188    4228    4409    4618    1733 
   Size:         21     667     767    1040     181     209    3345

  Fragments arranged by size: 

                3345    1040     767     667     209     181      21
 
BsrGI T'GTAC_A

Cuts at:   1541    1541 
   Size:       6230
 
BssHII G'CGCG_C

Cuts at:   2887    2887 
   Size:       6230
 
BssSI C'ACGA_G

Cuts at:   4501    4859    4501 
   Size:        358    5872
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1038    1116    1330    3607    1038 
   Size:         78     214    2277    3661

  Fragments arranged by size: 

                3661    2277     214      78
 
BstBI TT'CG_AA

Cuts at:   4591    4591 
   Size:       6230
 
BstXI CCAn_nnnn'nTGG

Cuts at:    309    1533    2566    3101     309 
   Size:       1224    1033     535    3438

  Fragments arranged by size: 

                3438    1224    1033     535
 
BstYI r'GATC_y

Cuts at:      1     939    1806    2780    2820    3255    3722    3745    4080 
   Size:        938     867     974      40     435     467      23     335

Cuts at:   4080    4326    5338    5424    5436    5782       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     974     938     867     467     449     435     346
                 335     246      86      40      23      12
 
BtgI C'CryG_G

Cuts at:    107     288     319     719     920    1844    1925    2903     107 
   Size:        181      31     400     201     924      81     978    3434

  Fragments arranged by size: 

                3434     978     924     400     201     181      81      31
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1263    2320    4180    4361    4524    1263 
   Size:       1057    1860     181     163    2969

  Fragments arranged by size: 

                2969    1860    1057     181     163
 
BtsI GCAGTG_nn'

Cuts at:    127    1331    2042    2872    3473     127 
   Size:       1204     711     830     601    2884

  Fragments arranged by size: 

                2884    1204     830     711     601
 
ClaI AT'CG_AT

Cuts at:   1481    1481 
   Size:       6230
 
DraI TTT'AAA

Cuts at:   3229    3229 
   Size:       6230
 
DrdI GACnn_nn'nnGTC

Cuts at:   2422    3936    4794    2422 
   Size:       1514     858    3858

  Fragments arranged by size: 

                3858    1514     858
 
EaeI y'GGCC_r

Cuts at:    110     299     387     612     917    1166    1353    2147    3155 
   Size:        189      88     225     305     249     187     794    1008

Cuts at:   3155    3190    3815    3989    4380    4407    5752     110 
   Size:         35     625     174     391      27    1345     588

  Fragments arranged by size: 

                1345    1008     794     625     588     391     305     249
                 225     189     187     174      88      35      27
 
EagI C'GGCC_G

Cuts at:    612    3815     612 
   Size:       3203    3027
 
EarI CTCTTCn'nnn_

Cuts at:   1606    2327    3209    4253    4463    1606 
   Size:        721     882    1044     210    3373

  Fragments arranged by size: 

                3373    1044     882     721     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1829    4438    4748    4894    6040    1829 
   Size:       2609     310     146    1146    2019

  Fragments arranged by size: 

                2609    2019    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1549    2556    3242    1549 
   Size:       1007     686    4537

  Fragments arranged by size: 

                4537    1007     686
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    169     173     393     519     696     779     858    2020    2113 
   Size:          4     220     126     177      83      79    1162      93

Cuts at:   2113    2656    2800    4055    4487    5234    5502     169 
   Size:        543     144    1255     432     747     268     897

  Fragments arranged by size: 

                1255    1162     897     747     543     432     268     220
                 177     144     126      93      83      79       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:    787    2161     787 
   Size:       1374    4856
 
EcoO109I rG'GnC_Cy

Cuts at:    172     238     331    1207    1345    1416    2129    2184    2530 
   Size:         66      93     876     138      71     713      55     346

Cuts at:   2530    2667    3369     172 
   Size:        137     702    3033

  Fragments arranged by size: 

                3033     876     713     702     346     138     137      93
                  71      66      55
 
EcoRI G'AATT_C

Cuts at:   3234    3234 
   Size:       6230
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3189    4249    3189 
   Size:       1060    5170
 
FspI TGC'GCA

Cuts at:   4011    4011 
   Size:       6230
 
HaeII r_GCGC'y

Cuts at:    317    1130    2171    2851    3664    3912    4934    5913     317 
   Size:        813    1041     680     813     248    1022     979     634

  Fragments arranged by size: 

                1041    1022     979     813     813     680     634     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    287     319     796     828    1017    1049    1098    1130    1389 
   Size:         32     477      32     189      32      49      32     259

Cuts at:   1389    1421    2335    2367    4072    4102    4104    4134     287 
   Size:         32     914      32    1705      30       2      30    2383

  Fragments arranged by size: 

                2383    1705     914     477     259     189      49      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1095    2697    3269    1095 
   Size:       1602     572    4056

  Fragments arranged by size: 

                4056    1602     572
 
KasI G'GCGC_C

Cuts at:    313    2167    2847    3660    3908     313 
   Size:       1854     680     813     248    2635

  Fragments arranged by size: 

                2635    1854     813     680     248
 
KpnI G_GTAC'C

Cuts at:   3250    3250 
   Size:       6230
 
MluI A'CGCG_T

Cuts at:   5663    5663 
   Size:       6230
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1653    2654    4901    5085    1653 
   Size:       1001    2247     184    2798

  Fragments arranged by size: 

                2798    2247    1001     184
 
MscI TGG'CCA

Cuts at:    112     301    3157    3991     112 
   Size:        189    2856     834    2351

  Fragments arranged by size: 

                2856    2351     834     189
 
MslI CAynn'nnrTG

Cuts at:    307     664    1324    1663    1843    1972    2549    2564    4219 
   Size:        357     660     339     180     129     577      15    1655

Cuts at:   4219    4346     307 
   Size:        127    2191

  Fragments arranged by size: 

                2191    1655     660     577     357     339     180     129
                 127      15
 
MspA1I CmG'CkG

Cuts at:    321     497    1728    2361    2525    2898    3063    3341    3655 
   Size:        176    1231     633     164     373     165     278     314

Cuts at:   3655    4015    5028    5273     321 
   Size:        360    1013     245    1278

  Fragments arranged by size: 

                1278    1231    1013     633     373     360     314     278
                 245     176     165     164
 
NaeI GCC'GGC

Cuts at:   1756    4411    1756 
   Size:       2655    3575
 
NarI GG'CG_CC

Cuts at:    314    2168    2848    3661    3909     314 
   Size:       1854     680     813     248    2635

  Fragments arranged by size: 

                2635    1854     813     680     248
 
NcoI C'CATG_G

Cuts at:    107     288    1844    2903     107 
   Size:        181    1556    1059    3434

  Fragments arranged by size: 

                3434    1556    1059     181
 
NgoMIV G'CCGG_C

Cuts at:   1754    4409    1754 
   Size:       2655    3575
 
NotI GC'GGCC_GC

Cuts at:    612     612 
   Size:       6230
 
NruI TCG'CGA

Cuts at:   2305    2305 
   Size:       6230
 
NspI r_CATG'y

Cuts at:   2181    3283    3783    4690    2181 
   Size:       1102     500     907    3721

  Fragments arranged by size: 

                3721    1102     907     500
 
PasI CC'CwG_GG

Cuts at:    327     990    1287    2163    2572    2661     327 
   Size:        663     297     876     409      89    3896

  Fragments arranged by size: 

                3896     876     663     409     297      89
 
PciI A'CATG_T

Cuts at:   4686    4686 
   Size:       6230
 
PflMI CCAn_nnn'nTGG

Cuts at:    477    1218    1368    1419    1581     477 
   Size:        741     150      51     162    5126

  Fragments arranged by size: 

                5126     741     162     150      51
 
PfoI T'CCnGG_A

Cuts at:   1418    1874    1997    2090    2610    2645    1418 
   Size:        456     123      93     520      35    5003

  Fragments arranged by size: 

                5003     520     456     123      93      35
 
PmeI GTTT'AAAC

Cuts at:   3229    3229 
   Size:       6230
 
PmlI CAC'GTG

Cuts at:    846     846 
   Size:       6230
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5369    5401    5369 
   Size:         32    6198
 
PpuMI rG'GwC_Cy

Cuts at:    172    1345    1416    2530     172 
   Size:       1173      71    1114    3872

  Fragments arranged by size: 

                3872    1173    1114      71
 
PspOMI G'GGCC_C

Cuts at:    330    2183     330 
   Size:       1853    4377
 
PspXI vC'TCGA_Gb

Cuts at:   2808    2808 
   Size:       6230
 
PstI C_TGCA'G

Cuts at:   1199    1727    2102    2366    2879    2975    3277    3962    1199 
   Size:        528     375     264     513      96     302     685    3467

  Fragments arranged by size: 

                3467     685     528     513     375     302     264      96
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6230
 
PvuII CAG'CTG

Cuts at:    497    1728    2361    2525    3063    3655    4015     497 
   Size:       1231     633     164     538     592     360    2712

  Fragments arranged by size: 

                2712    1231     633     592     538     360     164
 
RsrII CG'GwC_CG

Cuts at:   2424    4425    2424 
   Size:       2001    4229
 
SacI G_AGCT'C

Cuts at:   1551    2558    3244    1551 
   Size:       1007     686    4537

  Fragments arranged by size: 

                4537    1007     686
 
SacII CC_GC'GG

Cuts at:    322     322 
   Size:       6230
 
SalI G'TCGA_C

Cuts at:   3267    3267 
   Size:       6230
 
SanDI GG'GwC_CC

Cuts at:   1345    2530    1345 
   Size:       1185    5045
 
SapI GCTCTTCn'nnn_

Cuts at:   1606    4253    4463    1606 
   Size:       2647     210    3373

  Fragments arranged by size: 

                3373    2647     210
 
SbfI CC_TGCA'GG

Cuts at:   2879    3277    2879 
   Size:        398    5832
 
ScaI AGT'ACT

Cuts at:    628     889    5747     628 
   Size:        261    4858    1111

  Fragments arranged by size: 

                4858    1111     261
 
SexAI A'CCwGG_T

Cuts at:   1529    1529 
   Size:       6230
 
SfcI C'TryA_G

Cuts at:     33     950    1195    1723    2098    2362    2875    2971    3273 
   Size:        917     245     528     375     264     513      96     302

Cuts at:   3273    3958    4951    5142      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1121     993     917     685     528     513     375     302
                 264     245     191      96
 
SfoI GGC'GCC

Cuts at:    315    2169    2849    3662    3910     315 
   Size:       1854     680     813     248    2635

  Fragments arranged by size: 

                2635    1854     813     680     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6230
 
SmaI CCC'GGG

Cuts at:   3252    3252 
   Size:       6230
 
SmlI C'TyrA_G

Cuts at:    813    2704    2808    3385    4300    4792    5054     813 
   Size:       1891     104     577     915     492     262    1989

  Fragments arranged by size: 

                1989    1891     915     577     492     262     104
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6230
 
SphI G_CATG'C

Cuts at:   3283    3783    3283 
   Size:        500    5730
 
StuI AGG'CCT

Cuts at:   1567    2952    1567 
   Size:       1385    4845
 
StyI C'CwwG_G

Cuts at:    107     288     471    1844    2903    3074    3364    3718     107 
   Size:        181     183    1373    1059     171     290     354    2619

  Fragments arranged by size: 

                2619    1373    1059     354     290     183     181     171
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    144     477    1172    1210    1984    3093    3103    3112    3813 
   Size:        333     695      38     774    1109      10       9     701

Cuts at:   3813    3946    4405    4614     144 
   Size:        133     459     209    1760

  Fragments arranged by size: 

                1760    1109     774     701     695     459     333     209
                 133      38      10       9
 
TatI w'GTAC_w

Cuts at:    626     887    1541    2854    5745     626 
   Size:        261     654    1313    2891    1111

  Fragments arranged by size: 

                2891    1313    1111     654     261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    257     289     728     760     835     867    1979    2011    2522 
   Size:         32     439      32      75      32    1112      32     511

Cuts at:   2522    2554    3050    3082    5747    5779     257 
   Size:         32     496      32    2665      32     708

  Fragments arranged by size: 

                2665    1112     708     511     496     439      75      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4027    4027 
   Size:       6230
 
XbaI T'CTAG_A

Cuts at:   3261    3261 
   Size:       6230
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1284    1669    1800    3150    3725    1284 
   Size:        385     131    1350     575    3789

  Fragments arranged by size: 

                3789    1350     575     385     131
 
XhoI C'TCGA_G

Cuts at:    813    2808     813 
   Size:       1995    4235
 
XmaI C'CCGG_G

Cuts at:   3250    3250 
   Size:       6230
 
ZraI GAC'GTC

Cuts at:    507    1092     507 
   Size:        585    5645
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AloI     AscI    AvrII     BaeI     BcgI
    BfrBI     BmtI    BsiWI   BstEII  BstZ17I   Bsu36I    CspCI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NsiI     PacI    PshAI     PsiI     PsrI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XmnI
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