Restriction map for
pF1KB0496
(Circular) (Six-base) MAPSORT of: pF1KB0496.seq Check: 2492 from: 1 to: 6239
pF1KB0496 6239 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1156 1156
Size: 6239
AccI GT'mk_AC
Cuts at: 2563 3277 2563
Size: 714 5525
Acc65I G'GTAC_C
Cuts at: 3255 3255
Size: 6239
AclI AA'CG_TT
Cuts at: 246 2412 6079 246
Size: 2166 3667 406
Fragments arranged by size:
3667 2166 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1104 1544 2644 4064 4496 5243 5511 389
Size: 715 440 1100 1420 432 747 268 1117
Fragments arranged by size:
1420 1117 1100 747 715 440 432 268
AflII C'TTAA_G
Cuts at: 375 642 1762 375
Size: 267 1120 4852
Fragments arranged by size:
4852 1120 267
AflIII A'CryG_T
Cuts at: 1434 1830 1856 2673 3591 4695 5672 1434
Size: 396 26 817 918 1104 977 2001
Fragments arranged by size:
2001 1104 977 918 817 396 26
AgeI A'CCGG_T
Cuts at: 4627 4627
Size: 6239
AleI CACnn'nnGTG
Cuts at: 1475 1475
Size: 6239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1410 2936 1410
Size: 1526 4713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 502 534 502
Size: 32 6207
AlwNI CAG_nnn'CTG
Cuts at: 226 2248 3544 5111 226
Size: 2022 1296 1567 1354
Fragments arranged by size:
2022 1567 1354 1296
ApaLI G'TGCA_C
Cuts at: 5009 5009
Size: 6239
ApoI r'AATT_y
Cuts at: 721 866 1260 1511 1615 1926 2133 2232 2547
Size: 145 394 251 104 311 207 99 315
Cuts at: 2547 2856 3172 3243 5592 5848 721
Size: 309 316 71 2349 256 1112
Fragments arranged by size:
2349 1112 394 316 315 311 309 256
251 207 145 104 99 71
AseI AT'TA_AT
Cuts at: 20 761 1844 20
Size: 741 1083 4415
Fragments arranged by size:
4415 1083 741
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6239
AvaI C'yCGr_G
Cuts at: 3259 3259
Size: 6239
BamHI G'GATC_C
Cuts at: 2178 3264 2178
Size: 1086 5153
BanI G'GyrC_C
Cuts at: 3101 3255 3669 3917 3952 3101
Size: 154 414 248 35 5388
Fragments arranged by size:
5388 414 248 154 35
BanII G_rGCy'C
Cuts at: 1741 1771 2765 3253 4283 1741
Size: 30 994 488 1030 3697
Fragments arranged by size:
3697 1030 994 488 30
BbeI G_GCGC'C
Cuts at: 3105 3673 3921 3105
Size: 568 248 5423
Fragments arranged by size:
5423 568 248
BbsI GAAGACnn'nnnn_
Cuts at: 377 377
Size: 6239
BbvCI CC'TCA_GC
Cuts at: 597 597
Size: 6239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1138 1172 1138
Size: 34 6205
BciVI GTATCCnnnnn_n'
Cuts at: 4137 4898 6239 4137
Size: 761 1341 4137
Fragments arranged by size:
4137 1341 761
BclI T'GATC_A
Cuts at: 3759 3759
Size: 6239
BglII A'GATC_T
Cuts at: 3754 5791 3754
Size: 2037 4202
BlpI GC'TnA_GC
Cuts at: 3351 3351
Size: 6239
Bme1580I G_kGCm'C
Cuts at: 3864 3957 5013 3864
Size: 93 1056 5090
Fragments arranged by size:
5090 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2290 3451 3532 3867 6011 2290
Size: 1161 81 335 2144 2518
Fragments arranged by size:
2518 2144 1161 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 171 4606 171
Size: 4435 1804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 3053 203
Size: 2850 3389
Bpu10I CC'TnA_GC
Cuts at: 506 597 506
Size: 91 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3415 4294 4786 5084 3415
Size: 879 492 298 4570
Fragments arranged by size:
4570 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1178 1549 5943 1178
Size: 371 4394 1474
Fragments arranged by size:
4394 1474 371
BsaAI yAC'GTr
Cuts at: 1831 3592 1831
Size: 1761 4478
BsaBI GATnn'nnATC
Cuts at: 6 3753 5790 6
Size: 3747 2037 455
Fragments arranged by size:
3747 2037 455
BsaHI Gr'CG_yC
Cuts at: 3102 3670 3918 4620 6116 3102
Size: 568 248 702 1496 3225
Fragments arranged by size:
3225 1496 702 568 248
BsaWI w'CCGG_w
Cuts at: 3428 3949 4627 4901 5048 5459 3428
Size: 521 678 274 147 411 4208
Fragments arranged by size:
4208 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2114 2144 2114
Size: 30 6209
BseYI C'CCAG_C
Cuts at: 2117 3664 4422 4999 5495 2117
Size: 1547 758 577 496 2861
Fragments arranged by size:
2861 1547 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 572
Size: 6239
BsiEI CG_ry'CG
Cuts at: 104 2362 3781 3827 5035 104
Size: 2258 1419 46 1208 1308
Fragments arranged by size:
2258 1419 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2765 3253 4031 4221 5013 2765
Size: 488 778 190 792 3991
Fragments arranged by size:
3991 792 778 488 190
BsmI GAATG_Cn'
Cuts at: 118 2459 118
Size: 2341 3898
Bsp1286I G_dGCh'C
Cuts at: 1741 1771 2765 3253 3864 3957 4031 4221 4283
Size: 30 994 488 611 93 74 190 62
Cuts at: 4283 5013 1741
Size: 730 2967
Fragments arranged by size:
2967 994 730 611 488 190 93 74
62 30
BspEI T'CCGG_A
Cuts at: 5459 5459
Size: 6239
BspHI T'CATG_A
Cuts at: 5415 5415
Size: 6239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 2277 3289 3503 3805 4186 1156
Size: 1121 1012 214 302 381 3209
Fragments arranged by size:
3209 1121 1012 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4531 4585 6069 6234 50
Size: 4481 54 1484 165 55
Fragments arranged by size:
4481 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 629 1051 1151 4151 629
Size: 422 100 3000 2717
Fragments arranged by size:
3000 2717 422 100
BsrFI r'CCGG_y
Cuts at: 2361 4237 4418 4627 2361
Size: 1876 181 209 3973
Fragments arranged by size:
3973 1876 209 181
BsrGI T'GTAC_A
Cuts at: 436 436
Size: 6239
BssSI C'ACGA_G
Cuts at: 488 4510 4868 488
Size: 4022 358 1859
Fragments arranged by size:
4022 1859 358
BstAPI GCAn_nnn'nTGC
Cuts at: 457 2590 3616 457
Size: 2133 1026 3080
Fragments arranged by size:
3080 2133 1026
BstBI TT'CG_AA
Cuts at: 311 4600 311
Size: 4289 1950
BstXI CCAn_nnnn'nTGG
Cuts at: 636 636
Size: 6239
BstYI r'GATC_y
Cuts at: 1 2178 3264 3731 3754 4089 4335 5347 5433
Size: 2177 1086 467 23 335 246 1012 86
Cuts at: 5433 5445 5791 1
Size: 12 346 449
Fragments arranged by size:
2177 1086 1012 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 2564 2564
Size: 6239
Bsu36I CC'TnA_GG
Cuts at: 1424 1424
Size: 6239
BtgI C'CryG_G
Cuts at: 107 1473 1550 107
Size: 1366 77 4796
Fragments arranged by size:
4796 1366 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4189 4370 4533 4189
Size: 181 163 5895
Fragments arranged by size:
5895 181 163
BtsI GCAGTG_nn'
Cuts at: 161 2334 2757 3482 161
Size: 2173 423 725 2918
Fragments arranged by size:
2918 2173 725 423
DraI TTT'AAA
Cuts at: 2387 2994 3238 2387
Size: 607 244 5388
Fragments arranged by size:
5388 607 244
DrdI GACnn_nn'nnGTC
Cuts at: 2903 3128 3945 4803 2903
Size: 225 817 858 4339
Fragments arranged by size:
4339 858 817 225
EaeI y'GGCC_r
Cuts at: 1175 2359 3824 3998 4389 4416 5761 1175
Size: 1184 1465 174 391 27 1345 1653
Fragments arranged by size:
1653 1465 1345 1184 391 174 27
EagI C'GGCC_G
Cuts at: 2359 3824 2359
Size: 1465 4774
EarI CTCTTCn'nnn_
Cuts at: 350 458 1447 4262 4472 350
Size: 108 989 2815 210 2117
Fragments arranged by size:
2815 2117 989 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1212 4447 4757 4903 6049 127
Size: 1085 3235 310 146 1146 317
Fragments arranged by size:
3235 1146 1085 317 310 146
EcoICRI GAG'CTC
Cuts at: 2763 3251 2763
Size: 488 5751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 389 1104 1544 2644 3053 4064 4496 5243
Size: 186 715 440 1100 409 1011 432 747
Cuts at: 5243 5511 203
Size: 268 931
Fragments arranged by size:
1100 1011 931 747 715 440 432 409
268 186
EcoO109I rG'GnC_Cy
Cuts at: 214 3378 214
Size: 3164 3075
EcoRI G'AATT_C
Cuts at: 1926 2856 3243 1926
Size: 930 387 4922
Fragments arranged by size:
4922 930 387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3133 4258 3133
Size: 1125 5114
FspI TGC'GCA
Cuts at: 4020 4020
Size: 6239
HaeII r_GCGC'y
Cuts at: 3105 3673 3921 4943 5922 3105
Size: 568 248 1022 979 3422
Fragments arranged by size:
3422 1022 979 568 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1384 1416 1656 1688 2590 2622 4081 4111 4113
Size: 32 240 32 902 32 1459 30 2
Cuts at: 4113 4143 1384
Size: 30 3480
Fragments arranged by size:
3480 1459 902 240 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 561 3278 561
Size: 2717 3522
HindIII A'AGCT_T
Cuts at: 2501 2501
Size: 6239
KasI G'GCGC_C
Cuts at: 3101 3669 3917 3101
Size: 568 248 5423
Fragments arranged by size:
5423 568 248
KpnI G_GTAC'C
Cuts at: 3259 3259
Size: 6239
MfeI C'AATT_G
Cuts at: 1388 1745 1388
Size: 357 5882
MluI A'CGCG_T
Cuts at: 5672 5672
Size: 6239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 474 1290 1841 4910 5094 474
Size: 816 551 3069 184 1619
Fragments arranged by size:
3069 1619 816 551 184
MscI TGG'CCA
Cuts at: 1177 4000 1177
Size: 2823 3416
MslI CAynn'nnrTG
Cuts at: 1475 1663 4228 4355 1475
Size: 188 2565 127 3359
Fragments arranged by size:
3359 2565 188 127
MspA1I CmG'CkG
Cuts at: 601 1774 3350 3664 4024 5037 5282 601
Size: 1173 1576 314 360 1013 245 1558
Fragments arranged by size:
1576 1558 1173 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4420 4420
Size: 6239
NarI GG'CG_CC
Cuts at: 3102 3670 3918 3102
Size: 568 248 5423
Fragments arranged by size:
5423 568 248
NcoI C'CATG_G
Cuts at: 107 1473 107
Size: 1366 4873
NgoMIV G'CCGG_C
Cuts at: 4418 4418
Size: 6239
NspI r_CATG'y
Cuts at: 1438 1581 1860 3292 3792 4699 1438
Size: 143 279 1432 500 907 2978
Fragments arranged by size:
2978 1432 907 500 279 143
PciI A'CATG_T
Cuts at: 1434 1856 4695 1434
Size: 422 2839 2978
Fragments arranged by size:
2978 2839 422
PflMI CCAn_nnn'nTGG
Cuts at: 493 1747 493
Size: 1254 4985
PfoI T'CCnGG_A
Cuts at: 1902 1902
Size: 6239
PmeI GTTT'AAAC
Cuts at: 3238 3238
Size: 6239
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5378 5410 5378
Size: 32 6207
PshAI GACnn'nnGTC
Cuts at: 3067 3067
Size: 6239
PsiI TTA'TAA
Cuts at: 1988 1988
Size: 6239
PstI C_TGCA'G
Cuts at: 231 462 2814 3286 3971 231
Size: 231 2352 472 685 2499
Fragments arranged by size:
2499 2352 685 472 231
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6239
PvuII CAG'CTG
Cuts at: 3664 4024 3664
Size: 360 5879
RsrII CG'GwC_CG
Cuts at: 4434 4434
Size: 6239
SacI G_AGCT'C
Cuts at: 2765 3253 2765
Size: 488 5751
SalI G'TCGA_C
Cuts at: 3276 3276
Size: 6239
SapI GCTCTTCn'nnn_
Cuts at: 4262 4472 4262
Size: 210 6029
SbfI CC_TGCA'GG
Cuts at: 3286 3286
Size: 6239
ScaI AGT'ACT
Cuts at: 2201 5756 2201
Size: 3555 2684
SexAI A'CCwGG_T
Cuts at: 2073 2073
Size: 6239
SfcI C'TryA_G
Cuts at: 33 227 458 1505 2211 2240 2810 3282 3967
Size: 194 231 1047 706 29 570 472 685
Cuts at: 3967 4960 5151 33
Size: 993 191 1121
Fragments arranged by size:
1121 1047 993 706 685 570 472 231
194 191 29
SfoI GGC'GCC
Cuts at: 3103 3671 3919 3103
Size: 568 248 5423
Fragments arranged by size:
5423 568 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6239
SmaI CCC'GGG
Cuts at: 3261 3261
Size: 6239
SmlI C'TyrA_G
Cuts at: 375 642 1762 3394 4309 4801 5063 375
Size: 267 1120 1632 915 492 262 1551
Fragments arranged by size:
1632 1551 1120 915 492 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6239
SphI G_CATG'C
Cuts at: 3292 3792 3292
Size: 500 5739
SspI AAT'ATT
Cuts at: 665 665
Size: 6239
StuI AGG'CCT
Cuts at: 1540 1540
Size: 6239
StyI C'CwwG_G
Cuts at: 107 1473 3373 3727 107
Size: 1366 1900 354 2619
Fragments arranged by size:
2619 1900 1366 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3822 3955 4414 4623 3822
Size: 133 459 209 5438
Fragments arranged by size:
5438 459 209 133
TatI w'GTAC_w
Cuts at: 436 1437 1853 2199 5754 436
Size: 1001 416 346 3555 921
Fragments arranged by size:
3555 1001 921 416 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 5756 5788 2114
Size: 32 3610 32 2565
Fragments arranged by size:
3610 2565 32 32
Tth111I GACn'n_nGTC
Cuts at: 4036 4036
Size: 6239
XbaI T'CTAG_A
Cuts at: 3270 3270
Size: 6239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3228 3734 3228
Size: 506 5733
XmaI C'CCGG_G
Cuts at: 3259 3259
Size: 6239
XmnI GAAnn'nnTTC
Cuts at: 722 1630 1658 2036 2201 2520 3116 722
Size: 908 28 378 165 319 596 3845
Fragments arranged by size:
3845 908 596 378 319 165 28
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI ApaI AscI AvrII BaeI BfrBI
BglI BmgBI BmtI BseRI BsiWI BsmBI BssHII BstEII
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI