Restriction map for
pF1KB0497
(Circular) (Six-base) MAPSORT of: pF1KB0497.seq Check: 4658 from: 1 to: 16394
pF1KB0497 16394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2767 5498 6699 8694 11137 11838 2767
Size: 2731 1201 1995 2443 701 7323
Fragments arranged by size:
7323 2731 2443 1995 1201 701
AccI GT'mk_AC
Cuts at: 524 2301 2420 4339 6782 7286 7514 8918 11117
Size: 1777 119 1919 2443 504 228 1404 2199
Cuts at: 11117 11401 12769 13432 524
Size: 284 1368 663 3486
Fragments arranged by size:
3486 2443 2199 1919 1777 1404 1368 663
504 284 228 119
Acc65I G'GTAC_C
Cuts at: 13410 13410
Size: 16394
AclI AA'CG_TT
Cuts at: 13010 16234 13010
Size: 3224 13170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1153 3289 3370 3541 3751 4160 6140 7813
Size: 654 2136 81 171 210 409 1980 1673
Cuts at: 7813 8218 8263 8787 9634 9935 9949 10123 10369
Size: 405 45 524 847 301 14 174 246
Cuts at: 10369 10411 12977 14219 14651 15398 15666 499
Size: 42 2566 1242 432 747 268 1227
Fragments arranged by size:
2566 2136 1980 1673 1242 1227 847 747
654 524 432 409 405 301 268 246
210 174 171 81 45 42 14
AfeI AGC'GCT
Cuts at: 7912 11695 7912
Size: 3783 12611
AflII C'TTAA_G
Cuts at: 12214 12214
Size: 16394
AflIII A'CryG_T
Cuts at: 2987 3644 7439 7580 7889 8748 11980 12249 13746
Size: 657 3795 141 309 859 3232 269 1497
Cuts at: 13746 14850 15827 2987
Size: 1104 977 3554
Fragments arranged by size:
3795 3554 3232 1497 1104 977 859 657
309 269 141
AgeI A'CCGG_T
Cuts at: 12335 14782 12335
Size: 2447 13947
AhdI GACnn_n'nnGTC
Cuts at: 1919 5613 7723 13320 1919
Size: 3694 2110 5597 4993
Fragments arranged by size:
5597 4993 3694 2110
AleI CACnn'nnGTG
Cuts at: 10861 12979 10861
Size: 2118 14276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 233 4519 6715 9721 10690 11479 12470 13333 233
Size: 4286 2196 3006 969 789 991 863 3294
Fragments arranged by size:
4286 3294 3006 2196 991 969 863 789
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 928 960 6628 6660 928
Size: 32 5668 32 10662
Fragments arranged by size:
10662 5668 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1527 3309 3774 4314 4544 4768 5724 6006 7404
Size: 1782 465 540 230 224 956 282 1398
Cuts at: 7404 8418 8810 9105 9345 10005 10311 10347 10647
Size: 1014 392 295 240 660 306 36 300
Cuts at: 10647 10662 13699 15266 1527
Size: 15 3037 1567 2655
Fragments arranged by size:
3037 2655 1782 1567 1398 1014 956 660
540 465 392 306 300 295 282 240
230 224 36 15
ApaI G_GGCC'C
Cuts at: 604 4838 7705 9411 12921 604
Size: 4234 2867 1706 3510 4077
Fragments arranged by size:
4234 4077 3510 2867 1706
ApaLI G'TGCA_C
Cuts at: 1759 1849 15164 1759
Size: 90 13315 2989
Fragments arranged by size:
13315 2989 90
ApoI r'AATT_y
Cuts at: 918 2434 2749 3538 3610 5255 8656 13398 15747
Size: 1516 315 789 72 1645 3401 4742 2349
Cuts at: 15747 16003 918
Size: 256 1309
Fragments arranged by size:
4742 3401 2349 1645 1516 1309 789 315
256 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 16394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 16394
AvaI C'yCGr_G
Cuts at: 185 322 353 1247 2235 4869 5264 5424 6703
Size: 137 31 894 988 2634 395 160 1279
Cuts at: 6703 6943 8369 8693 8887 10265 12554 13414 185
Size: 240 1426 324 194 1378 2289 860 3165
Fragments arranged by size:
3165 2634 2289 1426 1378 1279 988 894
860 395 324 240 194 160 137 31
AvrII C'CTAG_G
Cuts at: 4479 6486 4479
Size: 2007 14387
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6939 6972 12202 12235 6939
Size: 33 5230 33 11098
Fragments arranged by size:
11098 5230 33 33
BamHI G'GATC_C
Cuts at: 6596 8039 8796 8813 11856 13419 6596
Size: 1443 757 17 3043 1563 9571
Fragments arranged by size:
9571 3043 1563 1443 757 17
BanI G'GyrC_C
Cuts at: 1053 1348 3242 3426 5895 6933 7275 7807 8631
Size: 295 1894 184 2469 1038 342 532 824
Cuts at: 8631 8706 11390 12272 13410 13824 14072 14107 1053
Size: 75 2684 882 1138 414 248 35 3340
Fragments arranged by size:
3340 2684 2469 1894 1138 1038 882 824
532 414 342 295 248 184 75 35
BanII G_rGCy'C
Cuts at: 257 323 604 1724 2373 3788 4838 5506 6863
Size: 66 281 1120 649 1415 1050 668 1357
Cuts at: 6863 7147 7705 8207 8543 9411 9521 9752 12921
Size: 284 558 502 336 868 110 231 3169
Cuts at: 12921 13166 13408 14438 257
Size: 245 242 1030 2213
Fragments arranged by size:
3169 2213 1415 1357 1120 1050 1030 868
668 649 558 502 336 284 281 245
242 231 110 66
BbeI G_GCGC'C
Cuts at: 1057 3246 12276 13828 14076 1057
Size: 2189 9030 1552 248 3375
Fragments arranged by size:
9030 3375 2189 1552 248
BbsI GAAGACnn'nnnn_
Cuts at: 1478 2526 2817 3477 3856 4493 7378 8052 8225
Size: 1048 291 660 379 637 2885 674 173
Cuts at: 8225 8251 12983 1478
Size: 26 4732 4889
Fragments arranged by size:
4889 4732 2885 1048 674 660 637 379
291 173 26
BbvCI CC'TCA_GC
Cuts at: 1536 6514 6851 8617 8782 9812 10602 1536
Size: 4978 337 1766 165 1030 790 7328
Fragments arranged by size:
7328 4978 1766 1030 790 337 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3385 3419 5673 5707 6244 6278 11362 11396 3385
Size: 34 2254 34 537 34 5084 34 8383
Fragments arranged by size:
8383 5084 2254 537 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1450 1944 3634 6774 10405 13085 14292 15053 16394
Size: 494 1690 3140 3631 2680 1207 761 1341
Cuts at: 16394 1450
Size: 1450
Fragments arranged by size:
3631 3140 2680 1690 1450 1341 1207 761
494
BclI T'GATC_A
Cuts at: 1199 1529 4089 5060 7541 7814 9575 13341 13914
Size: 330 2560 971 2481 273 1761 3766 573
Cuts at: 13914 1199
Size: 3679
Fragments arranged by size:
3766 3679 2560 2481 1761 971 573 330
273
BfrBI ATG'CAT
Cuts at: 962 7938 962
Size: 6976 9418
BglI GCCn_nnn'nGGC
Cuts at: 187 5522 6754 8428 9406 10945 12916 187
Size: 5335 1232 1674 978 1539 1971 3665
Fragments arranged by size:
5335 3665 1971 1674 1539 1232 978
BglII A'GATC_T
Cuts at: 653 2999 4166 6192 8960 9366 10127 13909 15946
Size: 2346 1167 2026 2768 406 761 3782 2037
Cuts at: 15946 653
Size: 1101
Fragments arranged by size:
3782 2768 2346 2037 2026 1167 1101 761
406
BlpI GC'TnA_GC
Cuts at: 251 2093 5326 9782 11884 12169 13506 251
Size: 1842 3233 4456 2102 285 1337 3139
Fragments arranged by size:
4456 3233 3139 2102 1842 1337 285
Bme1580I G_kGCm'C
Cuts at: 604 761 894 1353 1763 1853 4838 6045 6938
Size: 157 133 459 410 90 2985 1207 893
Cuts at: 6938 7280 7705 8300 8636 9411 10284 11658 12921
Size: 342 425 595 336 775 873 1374 1263
Cuts at: 12921 13363 14019 14112 15168 604
Size: 442 656 93 1056 1830
Fragments arranged by size:
2985 1830 1374 1263 1207 1056 893 873
775 656 595 459 442 425 410 342
336 157 133 93 90
BmgBI CAC'GTC
Cuts at: 1560 8552 11983 1560
Size: 6992 3431 5971
Fragments arranged by size:
6992 5971 3431
BmrI ACTGGGnnnn_n'
Cuts at: 1728 2055 2265 3328 3834 3963 4062 4401 5355
Size: 327 210 1063 506 129 99 339 954
Cuts at: 5355 7376 11319 11784 12273 13606 13687 14022 16166
Size: 2021 3943 465 489 1333 81 335 2144
Cuts at: 16166 1728
Size: 1956
Fragments arranged by size:
3943 2144 2021 1956 1333 1063 954 506
489 465 339 335 327 210 129 99
81
BmtI G_CTAG'C
Cuts at: 12643 12643
Size: 16394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 14761 14761
Size: 16394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 952 1402 1738 3271 3331 3469 3781 3796
Size: 180 450 336 1533 60 138 312 15
Cuts at: 3796 4756 5672 6853 7395 7624 9211 9433 9871
Size: 960 916 1181 542 229 1587 222 438
Cuts at: 9871 9955 10027 10669 10717 10780 10897 11092 11611
Size: 84 72 642 48 63 117 195 519
Cuts at: 11611 12370 12563 772
Size: 759 193 4603
Fragments arranged by size:
4603 1587 1533 1181 960 916 759 642
542 519 450 438 336 312 229 222
195 193 180 138 117 84 72 63
60 48 15
Bpu10I CC'TnA_GC
Cuts at: 272 1193 1536 3223 6514 6851 7574 8617 8622
Size: 921 343 1687 3291 337 723 1043 5
Cuts at: 8622 8782 9812 10602 10691 272
Size: 160 1030 790 89 5975
Fragments arranged by size:
5975 3291 1687 1043 1030 921 790 723
343 337 160 89 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 2227 2959 3151 5128 8527 10735 12592 13570
Size: 1949 732 192 1977 3399 2208 1857 978
Cuts at: 13570 14449 14941 15239 278
Size: 879 492 298 1433
Fragments arranged by size:
3399 2208 1977 1949 1857 1433 978 879
732 492 298 192
BsaI GGTCTCn'nnnn_
Cuts at: 838 3883 4675 5533 6238 6425 10591 10873 10882
Size: 3045 792 858 705 187 4166 282 9
Cuts at: 10882 12760 16098 838
Size: 1878 3338 1134
Fragments arranged by size:
4166 3338 3045 1878 1134 858 792 705
282 187 9
BsaAI yAC'GTr
Cuts at: 1728 2079 7518 11239 13747 1728
Size: 351 5439 3721 2508 4375
Fragments arranged by size:
5439 4375 3721 2508 351
BsaBI GATnn'nnATC
Cuts at: 6 11476 11861 13908 15945 6
Size: 11470 385 2047 2037 455
Fragments arranged by size:
11470 2047 2037 455 385
BsaHI Gr'CG_yC
Cuts at: 1054 1773 2068 3243 8409 10232 12273 13825 14073
Size: 719 295 1175 5166 1823 2041 1552 248
Cuts at: 14073 14775 16271 1054
Size: 702 1496 1177
Fragments arranged by size:
5166 2041 1823 1552 1496 1177 1175 719
702 295 248
BsaWI w'CCGG_w
Cuts at: 1741 4589 4982 12335 13133 13583 14104 14782 15056
Size: 2848 393 7353 798 450 521 678 274
Cuts at: 15056 15203 15614 1741
Size: 147 411 2521
Fragments arranged by size:
7353 2848 2521 798 678 521 450 411
393 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 928 958 3004 3034 6628 6658 10222 10252 11530
Size: 30 2046 30 3594 30 3564 30 1278
Cuts at: 11530 11560 13012 13042 13279 13309 928
Size: 30 1452 30 237 30 4013
Fragments arranged by size:
4013 3594 3564 2046 1452 1278 237 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 949 3133 3844 7114 9250 9910 10003 10024
Size: 669 2184 711 3270 2136 660 93 21
Cuts at: 10024 10714 11737 12208 280
Size: 690 1023 471 4466
Fragments arranged by size:
4466 3270 2184 2136 1023 711 690 669
660 471 93 21
BseYI C'CCAG_C
Cuts at: 489 1307 1754 3238 4837 7014 7592 8164 8530
Size: 818 447 1484 1599 2177 578 572 366
Cuts at: 8530 8826 9084 9465 9638 9943 10655 10662 10940
Size: 296 258 381 173 305 712 7 278
Cuts at: 10940 11690 13127 13819 14577 15154 15650 489
Size: 750 1437 692 758 577 496 1233
Fragments arranged by size:
2177 1599 1484 1437 1233 818 758 750
712 692 578 577 572 496 447 381
366 305 296 278 258 173 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 694 1012 1396 2236 2338 2476 3130 3202
Size: 93 318 384 840 102 138 654 72
Cuts at: 3202 3241 4465 4792 5476 6019 6345 6590 7489
Size: 39 1224 327 684 543 326 245 899
Cuts at: 7489 8749 8773 8826 9277 10456 10837 601
Size: 1260 24 53 451 1179 381 6158
Fragments arranged by size:
6158 1260 1224 1179 899 840 684 654
543 451 384 381 327 326 318 245
138 102 93 72 53 39 24
BsiEI CG_ry'CG
Cuts at: 104 9669 13936 13982 15190 104
Size: 9565 4267 46 1208 1308
Fragments arranged by size:
9565 4267 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 1763 1812 1853 3788 4597 7430 8315 8543
Size: 1506 49 41 1935 809 2833 885 228
Cuts at: 8543 8777 10970 11678 13408 14186 14376 15168 257
Size: 234 2193 708 1730 778 190 792 1483
Fragments arranged by size:
2833 2193 1935 1730 1506 1483 885 809
792 778 708 234 228 190 49 41
BsiWI C'GTAC_G
Cuts at: 7648 7648
Size: 16394
BsmI GAATG_Cn'
Cuts at: 222 1937 2114 6392 222
Size: 1715 177 4278 10224
Fragments arranged by size:
10224 4278 1715 177
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 9678 2059
Size: 7619 8775
Bsp1286I G_dGCh'C
Cuts at: 257 323 604 761 894 1353 1724 1763 1812
Size: 66 281 157 133 459 371 39 49
Cuts at: 1812 1853 2373 3788 4597 4838 5506 6045 6863
Size: 41 520 1415 809 241 668 539 818
Cuts at: 6863 6938 7147 7280 7430 7705 8207 8300 8315
Size: 75 209 133 150 275 502 93 15
Cuts at: 8315 8543 8636 8777 9411 9521 9752 10284 10970
Size: 228 93 141 634 110 231 532 686
Cuts at: 10970 11658 11678 12921 13166 13363 13408 14019 14112
Size: 688 20 1243 245 197 45 611 93
Cuts at: 14112 14186 14376 14438 15168 257
Size: 74 190 62 730 1483
Fragments arranged by size:
1483 1415 1243 818 809 730 688 686
668 634 611 539 532 520 502 459
371 281 275 245 241 231 228 209
197 190 157 150 141 133 133 110
93 93 93 75 74 66 62 49
45 41 39 20 15
BspEI T'CCGG_A
Cuts at: 1741 4589 4982 15614 1741
Size: 2848 393 10632 2521
Fragments arranged by size:
10632 2848 2521 393
BspHI T'CATG_A
Cuts at: 1160 1790 2513 5150 11357 13373 15570 1160
Size: 630 723 2637 6207 2016 2197 1984
Fragments arranged by size:
6207 2637 2197 2016 1984 723 630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1804 2117 2498 2767 4031 4739 5498 6699 8694
Size: 313 381 269 1264 708 759 1201 1995
Cuts at: 8694 8771 10370 10388 11137 11838 11916 12039 13325
Size: 77 1599 18 749 701 78 123 1286
Cuts at: 13325 13444 13658 13960 14341 1804
Size: 119 214 302 381 3857
Fragments arranged by size:
3857 1995 1599 1286 1264 1201 759 749
708 701 381 381 313 302 269 214
123 119 78 77 18
BsrBI CCG'CTC
Cuts at: 50 2052 5124 10686 12822 14686 14740 16224 16389
Size: 2002 3072 5562 2136 1864 54 1484 165
Cuts at: 16389 50
Size: 55
Fragments arranged by size:
5562 3072 2136 2002 1864 1484 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1957 3077 3198 4217 5482 5912 6749 11463 12224
Size: 1120 121 1019 1265 430 837 4714 761
Cuts at: 12224 14306 1957
Size: 2082 4045
Fragments arranged by size:
4714 4045 2082 1265 1120 1019 837 761
430 121
BsrFI r'CCGG_y
Cuts at: 3857 5429 5749 8069 10795 12229 12269 12335 14392
Size: 1572 320 2320 2726 1434 40 66 2057
Cuts at: 14392 14573 14782 3857
Size: 181 209 5469
Fragments arranged by size:
5469 2726 2320 2057 1572 1434 320 209
181 66 40
BsrGI T'GTAC_A
Cuts at: 501 2469 2576 5975 7892 501
Size: 1968 107 3399 1917 9003
Fragments arranged by size:
9003 3399 1968 1917 107
BssHII G'CGCG_C
Cuts at: 1750 1750
Size: 16394
BssSI C'ACGA_G
Cuts at: 3337 3394 3818 9952 11381 11617 14665 15023 3337
Size: 57 424 6134 1429 236 3048 358 4708
Fragments arranged by size:
6134 4708 3048 1429 424 358 236 57
BstAPI GCAn_nnn'nTGC
Cuts at: 959 1419 1572 2571 7404 8310 8772 9105 11472
Size: 460 153 999 4833 906 462 333 2367
Cuts at: 11472 13771 959
Size: 2299 3582
Fragments arranged by size:
4833 3582 2367 2299 999 906 462 460
333 153
BstBI TT'CG_AA
Cuts at: 1893 3608 6878 11144 14755 1893
Size: 1715 3270 4266 3611 3532
Fragments arranged by size:
4266 3611 3532 3270 1715
BstEII G'GTnAC_C
Cuts at: 2505 4962 8469 2505
Size: 2457 3507 10430
Fragments arranged by size:
10430 3507 2457
BstXI CCAn_nnnn'nTGG
Cuts at: 3636 3687 4828 5773 6354 7347 9054 11190 11441
Size: 51 1141 945 581 993 1707 2136 251
Cuts at: 11441 12888 3636
Size: 1447 7142
Fragments arranged by size:
7142 2136 1707 1447 1141 993 945 581
251 51
BstYI r'GATC_y
Cuts at: 1 653 1079 1647 2228 2915 2999 3380 4073
Size: 652 426 568 581 687 84 381 693
Cuts at: 4073 4166 5075 5355 5720 5807 6093 6192 6596
Size: 93 909 280 365 87 286 99 404
Cuts at: 6596 8039 8229 8666 8796 8813 8960 9366 10127
Size: 1443 190 437 130 17 147 406 761
Cuts at: 10127 10769 11856 12599 12683 13419 13886 13909 14244
Size: 642 1087 743 84 736 467 23 335
Cuts at: 14244 14490 15502 15588 15600 15946 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1443 1087 1012 909 761 743 736 693
687 652 642 581 568 467 449 437
426 406 404 381 365 346 335 286
280 246 190 147 130 99 93 87
86 84 84 23 17 12
BstZ17I GTA'TAC
Cuts at: 2302 2421 11402 2302
Size: 119 8981 7294
Fragments arranged by size:
8981 7294 119
Bsu36I CC'TnA_GG
Cuts at: 5002 10298 12431 5002
Size: 5296 2133 8965
Fragments arranged by size:
8965 5296 2133
BtgI C'CryG_G
Cuts at: 277 3884 3893 3900 5477 5618 5888 6614 7040
Size: 3607 9 7 1577 141 270 726 426
Cuts at: 7040 7358 7697 7901 8789 9713 11349 277
Size: 318 339 204 888 924 1636 5322
Fragments arranged by size:
5322 3607 1636 1577 924 888 726 426
339 318 270 204 141 9 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3016 4955 6126 6678 6712 7365 10713 11440 11718
Size: 1939 1171 552 34 653 3348 727 278
Cuts at: 11718 14344 14525 14688 3016
Size: 2626 181 163 4722
Fragments arranged by size:
4722 3348 2626 1939 1171 727 653 552
278 181 163 34
BtsI GCAGTG_nn'
Cuts at: 389 2572 2635 3186 5063 9235 9379 12974 13361
Size: 2183 63 551 1877 4172 144 3595 387
Cuts at: 13361 13637 389
Size: 276 3146
Fragments arranged by size:
4172 3595 3146 2183 1877 551 387 276
144 63
ClaI AT'CG_AT
Cuts at: 10169 10169
Size: 16394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5895 5930 12225 12260 5895
Size: 35 6295 35 10029
Fragments arranged by size:
10029 6295 35 35
DraI TTT'AAA
Cuts at: 13393 13393
Size: 16394
DraIII CAC_nnn'GTG
Cuts at: 304 1572 2294 5102 6626 8772 11778 12078 304
Size: 1268 722 2808 1524 2146 3006 300 4620
Fragments arranged by size:
4620 3006 2808 2146 1524 1268 722 300
DrdI GACnn_nn'nnGTC
Cuts at: 1122 8414 10717 14100 14958 1122
Size: 7292 2303 3383 858 2558
Fragments arranged by size:
7292 3383 2558 2303 858
EaeI y'GGCC_r
Cuts at: 144 197 1708 3362 3689 3855 5049 5838 5963
Size: 53 1511 1654 327 166 1194 789 125
Cuts at: 5963 6755 6989 7325 9666 12441 12578 12878 13979
Size: 792 234 336 2341 2775 137 300 1101
Cuts at: 13979 14153 14544 14571 15916 144
Size: 174 391 27 1345 622
Fragments arranged by size:
2775 2341 1654 1511 1345 1194 1101 792
789 622 391 336 327 300 234 174
166 137 125 53 27
EagI C'GGCC_G
Cuts at: 9666 13979 9666
Size: 4313 12081
EarI CTCTTCn'nnn_
Cuts at: 349 1273 2435 2698 3066 3695 6101 6832 7105
Size: 924 1162 263 368 629 2406 731 273
Cuts at: 7105 7838 7951 8320 8657 8734 9368 9389 9911
Size: 733 113 369 337 77 634 21 522
Cuts at: 9911 12554 12776 13219 14417 14627 349
Size: 2643 222 443 1198 210 2116
Fragments arranged by size:
2643 2406 2116 1198 1162 924 733 731
634 629 522 443 369 368 337 273
263 222 210 113 77 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 5365 5866 7603 8806 11863 14602 14912 15058
Size: 4974 501 1737 1203 3057 2739 310 146
Cuts at: 15058 16204 391
Size: 1146 581
Fragments arranged by size:
4974 3057 2739 1737 1203 1146 581 501
310 146
EcoICRI GAG'CTC
Cuts at: 255 3786 8541 13406 255
Size: 3531 4755 4865 3243
Fragments arranged by size:
4865 4755 3531 3243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 772 952 1153 1402 1738 3271 3289 3331
Size: 273 180 201 249 336 1533 18 42
Cuts at: 3331 3370 3469 3541 3751 3781 3796 4160 4756
Size: 39 99 72 210 30 15 364 596
Cuts at: 4756 5672 6140 6853 7395 7624 7813 8218 8263
Size: 916 468 713 542 229 189 405 45
Cuts at: 8263 8787 9211 9433 9634 9871 9935 9949 9955
Size: 524 424 222 201 237 64 14 6
Cuts at: 9955 10027 10123 10369 10411 10669 10717 10780 10897
Size: 72 96 246 42 258 48 63 117
Cuts at: 10897 11092 11611 12370 12563 12977 14219 14651 15398
Size: 195 519 759 193 414 1242 432 747
Cuts at: 15398 15666 499
Size: 268 1227
Fragments arranged by size:
1533 1242 1227 916 759 747 713 596
542 524 519 468 432 424 414 405
364 336 273 268 258 249 246 237
229 222 210 201 201 195 193 189
180 117 99 96 72 72 64 63
48 45 42 42 39 30 18 15
14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1593 2494 3778 5557 6301 8887 9394 10009 11494
Size: 901 1284 1779 744 2586 507 615 1485
Cuts at: 11494 11716 11752 12676 1593
Size: 222 36 924 5311
Fragments arranged by size:
5311 2586 1779 1485 1284 924 901 744
615 507 222 36
EcoO109I rG'GnC_Cy
Cuts at: 289 429 600 601 925 1327 1503 2106 2190
Size: 140 171 1 324 402 176 603 84
Cuts at: 2190 2427 3762 4834 4918 4974 5515 5550 5562
Size: 237 1335 1072 84 56 541 35 12
Cuts at: 5562 5601 6735 7354 7589 7702 8880 9408 9626
Size: 39 1134 619 235 113 1178 528 218
Cuts at: 9626 10002 10014 10072 10308 10344 10644 11727 11961
Size: 376 12 58 236 36 300 1083 234
Cuts at: 11961 12567 13104 13533 289
Size: 606 537 429 3150
Fragments arranged by size:
3150 1335 1178 1134 1083 1072 619 606
603 541 537 528 429 402 376 324
300 237 236 235 234 218 176 171
140 113 84 84 58 56 39 36
35 12 12 1
EcoRI G'AATT_C
Cuts at: 2749 3610 13398 2749
Size: 861 9788 5745
Fragments arranged by size:
9788 5745 861
EcoRV GAT'ATC
Cuts at: 8508 9660 8508
Size: 1152 15242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 360 3039 3375 14413 360
Size: 2679 336 11038 2341
Fragments arranged by size:
11038 2679 2341 336
FspI TGC'GCA
Cuts at: 2113 10513 14175 2113
Size: 8400 3662 4332
Fragments arranged by size:
8400 4332 3662
FspAI rTGC'GCAy
Cuts at: 10513 10513
Size: 16394
HaeII r_GCGC'y
Cuts at: 1057 3045 3246 6081 7242 7914 11697 12276 13232
Size: 1988 201 2835 1161 672 3783 579 956
Cuts at: 13232 13828 14076 15098 16077 1057
Size: 596 248 1022 979 1374
Fragments arranged by size:
3783 2835 1988 1374 1161 1022 979 956
672 596 579 248 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 723 755 1900 1905 1932 1937 3993 4025 4081
Size: 32 1145 5 27 5 2056 32 56
Cuts at: 4081 4113 4475 4507 4584 4616 5599 5631 5686
Size: 32 362 32 77 32 983 32 55
Cuts at: 5686 5718 6697 6729 7709 7741 8002 8034 8492
Size: 32 979 32 980 32 261 32 458
Cuts at: 8492 8524 8656 8688 9433 9465 9583 9615 10222
Size: 32 132 32 745 32 118 32 607
Cuts at: 10222 10254 10281 10313 11600 11632 11805 11837 12162
Size: 32 27 32 1287 32 173 32 325
Cuts at: 12162 12194 12451 12483 13012 13044 13279 13306 13311
Size: 32 257 32 529 32 235 27 5
Cuts at: 13311 13326 13338 13358 14236 14266 14268 14298 723
Size: 15 12 20 878 30 2 30 2819
Fragments arranged by size:
2819 2056 1287 1145 983 980 979 878
745 607 529 458 362 325 261 257
235 173 132 118 77 56 55 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 27 27
27 20 15 12 5 5 5 2
HincII GTy'rAC
Cuts at: 1227 2001 4536 5589 6671 7287 7437 10107 10623
Size: 774 2535 1053 1082 616 150 2670 516
Cuts at: 10623 13433 1227
Size: 2810 4188
Fragments arranged by size:
4188 2810 2670 2535 1082 1053 774 616
516 150
HindIII A'AGCT_T
Cuts at: 4104 5281 7825 4104
Size: 1177 2544 12673
Fragments arranged by size:
12673 2544 1177
KasI G'GCGC_C
Cuts at: 1053 3242 12272 13824 14072 1053
Size: 2189 9030 1552 248 3375
Fragments arranged by size:
9030 3375 2189 1552 248
KpnI G_GTAC'C
Cuts at: 13414 13414
Size: 16394
MfeI C'AATT_G
Cuts at: 5882 12792 5882
Size: 6910 9484
MluI A'CGCG_T
Cuts at: 8748 15827 8748
Size: 7079 9315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 1928 3513 4809 5255 7183 8993 9164 10601
Size: 1665 1585 1296 446 1928 1810 171 1437
Cuts at: 10601 12167 15065 15249 263
Size: 1566 2898 184 1408
Fragments arranged by size:
2898 1928 1810 1665 1585 1566 1437 1408
1296 446 184 171
MscI TGG'CCA
Cuts at: 199 1710 3364 3691 5051 5965 6991 7327 12443
Size: 1511 1654 327 1360 914 1026 336 5116
Cuts at: 12443 12880 14155 199
Size: 437 1275 2438
Fragments arranged by size:
5116 2438 1654 1511 1360 1275 1026 914
437 336 327
MslI CAynn'nnrTG
Cuts at: 793 2498 3191 3685 4960 5695 5771 5818 5893
Size: 1705 693 494 1275 735 76 47 75
Cuts at: 5893 6352 6697 7549 7933 8563 9032 9253 9703
Size: 459 345 852 384 630 469 221 450
Cuts at: 9703 10672 10861 11177 11683 11938 12452 12703 12763
Size: 969 189 316 506 255 514 251 60
Cuts at: 12763 12886 12979 14383 14510 793
Size: 123 93 1404 127 2677
Fragments arranged by size:
2677 1705 1404 1275 969 852 735 693
630 514 506 494 469 459 450 384
345 316 255 251 221 189 127 123
93 76 75 60 47
MspA1I CmG'CkG
Cuts at: 990 1307 2467 2936 4080 4503 6006 6371 6770
Size: 317 1160 469 1144 423 1503 365 399
Cuts at: 6770 6953 7018 8405 10051 10659 10821 10875 10944
Size: 183 65 1387 1646 608 162 54 69
Cuts at: 10944 11667 11844 12020 12583 13505 13819 14179 15192
Size: 723 177 176 563 922 314 360 1013
Cuts at: 15192 15437 990
Size: 245 1947
Fragments arranged by size:
1947 1646 1503 1387 1160 1144 1013 922
723 608 563 469 423 399 365 360
317 314 245 183 177 176 162 69
65 54
NaeI GCC'GGC
Cuts at: 5431 5751 12231 14575 5431
Size: 320 6480 2344 7250
Fragments arranged by size:
7250 6480 2344 320
NarI GG'CG_CC
Cuts at: 1054 3243 12273 13825 14073 1054
Size: 2189 9030 1552 248 3375
Fragments arranged by size:
9030 3375 2189 1552 248
NcoI C'CATG_G
Cuts at: 3884 5618 5888 7040 7697 8789 9713 3884
Size: 1734 270 1152 657 1092 924 10565
Fragments arranged by size:
10565 1734 1152 1092 924 657 270
NdeI CA'TA_TG
Cuts at: 11174 11174
Size: 16394
NgoMIV G'CCGG_C
Cuts at: 5429 5749 12229 14573 5429
Size: 320 6480 2344 7250
Fragments arranged by size:
7250 6480 2344 320
NheI G'CTAG_C
Cuts at: 12639 12639
Size: 16394
NruI TCG'CGA
Cuts at: 3214 6697 3214
Size: 3483 12911
NsiI A_TGCA'T
Cuts at: 964 7940 964
Size: 6976 9418
NspI r_CATG'y
Cuts at: 498 1659 1992 2308 2991 3648 4150 6569 6876
Size: 1161 333 316 683 657 502 2419 307
Cuts at: 6876 7443 7584 7893 7986 8382 9702 12253 13242
Size: 567 141 309 93 396 1320 2551 989
Cuts at: 13242 13447 13947 14854 498
Size: 205 500 907 2038
Fragments arranged by size:
2551 2419 2038 1320 1161 989 907 683
657 567 502 500 396 333 316 309
307 205 141 93
PasI CC'CwG_GG
Cuts at: 1353 5565 7708 11712 1353
Size: 4212 2143 4004 6035
Fragments arranged by size:
6035 4212 4004 2143
PciI A'CATG_T
Cuts at: 2987 3644 7439 7580 7889 12249 14850 2987
Size: 657 3795 141 309 4360 2601 4531
Fragments arranged by size:
4531 4360 3795 2601 657 309 141
PflMI CCAn_nnn'nTGG
Cuts at: 557 1152 2079 2363 2502 4229 4314 6527 7003
Size: 595 927 284 139 1727 85 2213 476
Cuts at: 7003 7418 8418 8855 10005 10662 12876 557
Size: 415 1000 437 1150 657 2214 4075
Fragments arranged by size:
4075 2214 2213 1727 1150 1000 927 657
595 476 437 415 284 139 85
PfoI T'CCnGG_A
Cuts at: 1082 1442 2216 3248 3446 4064 4613 4643 10199
Size: 360 774 1032 198 618 549 30 5556
Cuts at: 10199 1082
Size: 7277
Fragments arranged by size:
7277 5556 1032 774 618 549 360 198
30
PmeI GTTT'AAAC
Cuts at: 13393 13393
Size: 16394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 960 4388 4420 6628 6660 15533 15565 928
Size: 32 3428 32 2208 32 8873 32 1757
Fragments arranged by size:
8873 3428 2208 1757 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 289 429 925 1327 1503 2106 2427 3762 4974
Size: 140 496 402 176 603 321 1335 1212
Cuts at: 4974 5550 6735 7354 7589 10014 10072 12567 289
Size: 576 1185 619 235 2425 58 2495 4116
Fragments arranged by size:
4116 2495 2425 1335 1212 1185 619 603
576 496 402 321 235 176 140 58
PspOMI G'GGCC_C
Cuts at: 600 4834 7701 9407 12917 600
Size: 4234 2867 1706 3510 4077
Fragments arranged by size:
4234 4077 3510 2867 1706
PspXI vC'TCGA_Gb
Cuts at: 8887 8887
Size: 16394
PstI C_TGCA'G
Cuts at: 248 783 2768 2966 3875 5523 7400 10367 11666
Size: 535 1985 198 909 1648 1877 2967 1299
Cuts at: 11666 11849 12107 12680 12989 13212 13441 14126 248
Size: 183 258 573 309 223 229 685 2516
Fragments arranged by size:
2967 2516 1985 1877 1648 1299 909 685
573 535 309 258 229 223 198 183
PvuI CG_AT'CG
Cuts at: 104 104
Size: 16394
PvuII CAG'CTG
Cuts at: 990 2467 4503 6006 7018 10051 10659 10821 10875
Size: 1477 2036 1503 1012 3033 608 162 54
Cuts at: 10875 11667 11844 13819 14179 990
Size: 792 177 1975 360 3205
Fragments arranged by size:
3205 3033 2036 1975 1503 1477 1012 792
608 360 177 162 54
RsrII CG'GwC_CG
Cuts at: 14589 14589
Size: 16394
SacI G_AGCT'C
Cuts at: 257 3788 8543 13408 257
Size: 3531 4755 4865 3243
Fragments arranged by size:
4865 4755 3531 3243
SalI G'TCGA_C
Cuts at: 7285 13431 7285
Size: 6146 10248
SanDI GG'GwC_CC
Cuts at: 1327 7354 7589 10072 1327
Size: 6027 235 2483 7649
Fragments arranged by size:
7649 6027 2483 235
SapI GCTCTTCn'nnn_
Cuts at: 1273 8320 13219 14417 14627 1273
Size: 7047 4899 1198 210 3040
Fragments arranged by size:
7047 4899 3040 1198 210
SbfI CC_TGCA'GG
Cuts at: 5523 13441 5523
Size: 7918 8476
ScaI AGT'ACT
Cuts at: 5413 15911 5413
Size: 10498 5896
SexAI A'CCwGG_T
Cuts at: 4058 4325 4529 5162 7307 9242 10025 4058
Size: 267 204 633 2145 1935 783 10427
Fragments arranged by size:
10427 2145 1935 783 633 267 204
SfcI C'TryA_G
Cuts at: 33 244 434 779 2764 2962 3871 4196 5519
Size: 211 190 345 1985 198 909 325 1323
Cuts at: 5519 6085 6397 7396 10363 10481 10587 11118 11662
Size: 566 312 999 2967 118 106 531 544
Cuts at: 11662 11845 12103 12676 12985 13208 13437 14122 15115
Size: 183 258 573 309 223 229 685 993
Cuts at: 15115 15306 33
Size: 191 1121
Fragments arranged by size:
2967 1985 1323 1121 999 993 909 685
573 566 544 531 345 325 312 309
258 229 223 211 198 191 190 183
118 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 6754 9406 12916 6754
Size: 2652 3510 10232
Fragments arranged by size:
10232 3510 2652
SfoI GGC'GCC
Cuts at: 1055 3244 12274 13826 14074 1055
Size: 2189 9030 1552 248 3375
Fragments arranged by size:
9030 3375 2189 1552 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 16394
SmaI CCC'GGG
Cuts at: 2237 5266 8695 12556 13416 2237
Size: 3029 3429 3861 860 5215
Fragments arranged by size:
5215 3861 3429 3029 860
SmlI C'TyrA_G
Cuts at: 293 2242 2974 3166 5143 8542 8887 10750 12214
Size: 1949 732 192 1977 3399 345 1863 1464
Cuts at: 12214 12571 13549 14464 14956 15218 293
Size: 357 978 915 492 262 1469
Fragments arranged by size:
3399 1977 1949 1863 1469 1464 978 915
732 492 357 345 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 16394
SphI G_CATG'C
Cuts at: 498 1992 7986 13447 13947 498
Size: 1494 5994 5461 500 2945
Fragments arranged by size:
5994 5461 2945 1494 500
StuI AGG'CCT
Cuts at: 3551 4369 4610 6403 12436 3551
Size: 818 241 1793 6033 7509
Fragments arranged by size:
7509 6033 1793 818 241
StyI C'CwwG_G
Cuts at: 260 1169 2651 3758 3884 4112 4479 5618 5888
Size: 909 1482 1107 126 228 367 1139 270
Cuts at: 5888 6302 6473 6486 7040 7505 7697 7735 7967
Size: 414 171 13 554 465 192 38 232
Cuts at: 7967 8207 8789 8831 9713 9776 10215 11541 12202
Size: 240 582 42 882 63 439 1326 661
Cuts at: 12202 12368 13082 13232 13528 13882 260
Size: 166 714 150 296 354 2772
Fragments arranged by size:
2772 1482 1326 1139 1107 909 882 714
661 582 554 465 439 414 367 354
296 270 240 232 228 192 171 166
150 126 63 42 38 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 2772 3962 4612 4619 5022 5551 5773 8587
Size: 1026 1190 650 7 403 529 222 2814
Cuts at: 8587 9057 9693 11766 11915 12546 13304 13977 14110
Size: 470 636 2073 149 631 758 673 133
Cuts at: 14110 14569 14778 1746
Size: 459 209 3362
Fragments arranged by size:
3362 2814 2073 1190 1026 758 673 650
636 631 529 470 459 403 222 209
149 133 7
TatI w'GTAC_w
Cuts at: 501 2469 2576 4460 4883 5411 5630 5975 6947
Size: 1968 107 1884 423 528 219 345 972
Cuts at: 6947 6977 7667 7892 13241 15909 501
Size: 30 690 225 5349 2668 986
Fragments arranged by size:
5349 2668 1968 1884 986 972 690 528
423 345 225 219 107 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 1003 1035 1064 1096 2794 2826 5835
Size: 32 601 32 29 32 1698 32 3009
Cuts at: 5835 5867 6552 6584 6610 6642 9319 9351 10403
Size: 32 685 32 26 32 2677 32 1052
Cuts at: 10403 10435 11692 11724 11762 11794 15911 15943 370
Size: 32 1257 32 38 32 4117 32 821
Fragments arranged by size:
4117 3009 2677 1698 1257 1052 821 685
601 38 32 32 32 32 32 32
32 32 32 32 32 32 29 26
Tth111I GACn'n_nGTC
Cuts at: 1786 3442 4060 4531 8550 14191 1786
Size: 1656 618 471 4019 5641 3989
Fragments arranged by size:
5641 4019 3989 1656 618 471
XbaI T'CTAG_A
Cuts at: 9369 13425 9369
Size: 4056 12338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1884 6028 6351 7347 7863 9051 9207 9408 11288
Size: 4144 323 996 516 1188 156 201 1880
Cuts at: 11288 12459 12860 13889 1884
Size: 1171 401 1029 4389
Fragments arranged by size:
4389 4144 1880 1188 1171 1029 996 516
401 323 201 156
XhoI C'TCGA_G
Cuts at: 8887 8887
Size: 16394
XmaI C'CCGG_G
Cuts at: 2235 5264 8693 12554 13414 2235
Size: 3029 3429 3861 860 5215
Fragments arranged by size:
5215 3861 3429 3029 860
XmnI GAAnn'nnTTC
Cuts at: 2335 3208 4474 4652 5924 6110 2335
Size: 873 1266 178 1272 186 12619
Fragments arranged by size:
12619 1272 1266 873 186 178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AscI FseI HpaI NotI PacI PmlI PshAI
PsiI PsrI SacII SgrAI SnaBI SrfI SspI SwaI
ZraI