Restriction map for
pF1KB0498
(Circular) (Six-base) MAPSORT of: pF1KB0498.seq Check: 5294 from: 1 to: 4367
pF1KB0498 4367 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 759 759
Size: 4367
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 580 2192 2624 3371 3639 472
Size: 108 1612 432 747 268 1200
Fragments arranged by size:
1612 1200 747 432 268 108
AfeI AGC'GCT
Cuts at: 1284 1284
Size: 4367
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 246 1672 3239 246
Size: 1426 1567 1374
Fragments arranged by size:
1567 1426 1374
ApaI G_GGCC'C
Cuts at: 253 253
Size: 4367
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 1371 3720 3976 1371
Size: 2349 256 1762
Fragments arranged by size:
2349 1762 256
AseI AT'TA_AT
Cuts at: 20 323 1069 20
Size: 303 746 3318
Fragments arranged by size:
3318 746 303
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 1383 1797 2045 2080 1383
Size: 414 248 35 3670
Fragments arranged by size:
3670 414 248 35
BanII G_rGCy'C
Cuts at: 253 570 844 1381 2411 253
Size: 317 274 537 1030 2209
Fragments arranged by size:
2209 1030 537 317 274
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 1091 1091
Size: 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 919 953 919
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglII A'GATC_T
Cuts at: 690 1882 3919 690
Size: 1192 2037 1138
Fragments arranged by size:
2037 1192 1138
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 253 363 1992 2085 3141 253
Size: 110 1629 93 1056 1479
Fragments arranged by size:
1629 1479 1056 110 93
BmgBI CAC'GTC
Cuts at: 142 142
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 1317 1579 1660 1995 4139 1317
Size: 262 81 335 2144 1545
Fragments arranged by size:
2144 1545 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1054
Size: 4367
Bpu10I CC'TnA_GC
Cuts at: 838 838
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1046 1543 2422 2914 3212 487
Size: 559 497 879 492 298 1642
Fragments arranged by size:
1642 879 559 497 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 420 1720 420
Size: 1300 3067
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 1798 2046 2748 4244 1798
Size: 248 702 1496 1921
Fragments arranged by size:
1921 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 811 841 811
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 724 724
Size: 4367
BseYI C'CCAG_C
Cuts at: 246 356 790 1158 1792 2550 3127 3623 246
Size: 110 434 368 634 758 577 496 990
Fragments arranged by size:
990 758 634 577 496 434 368 110
BsiEI CG_ry'CG
Cuts at: 104 736 1909 1955 3163 104
Size: 632 1173 46 1208 1308
Fragments arranged by size:
1308 1208 1173 632 46
BsiHKAI G_wGCw'C
Cuts at: 844 1381 2159 2349 3141 844
Size: 537 778 190 792 2070
Fragments arranged by size:
2070 792 778 537 190
BsmI GAATG_Cn'
Cuts at: 591 591
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 253 363 570 844 1381 1992 2085 2159 2349
Size: 110 207 274 537 611 93 74 190
Cuts at: 2349 2411 3141 253
Size: 62 730 1479
Fragments arranged by size:
1479 730 611 537 274 207 190 110
93 74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 468 3543 468
Size: 3075 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 759 924 1417 1631 1933 2314 759
Size: 165 493 214 302 381 2812
Fragments arranged by size:
2812 493 381 302 214 165
BsrBI CCG'CTC
Cuts at: 50 918 2659 2713 4197 4362 50
Size: 868 1741 54 1484 165 55
Fragments arranged by size:
1741 1484 868 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 807 2365 2546 2755 807
Size: 1558 181 209 2419
Fragments arranged by size:
2419 1558 209 181
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstEII G'GTnAC_C
Cuts at: 771 1200 771
Size: 429 3938
BstYI r'GATC_y
Cuts at: 1 690 1258 1392 1859 1882 2217 2463 3475
Size: 689 568 134 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 689 568 467 449 346 335 246
134 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 235 571 235
Size: 336 4031
BtgI C'CryG_G
Cuts at: 851 851
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1610 1610
Size: 4367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 632 667 632
Size: 35 4332
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 329 1952 2126 2517 2544 3889 329
Size: 1623 174 391 27 1345 807
Fragments arranged by size:
1623 1345 807 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 203 676 958 1318 2390 2600 203
Size: 473 282 360 1072 210 1970
Fragments arranged by size:
1970 1072 473 360 282 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 296 826 837 2575 2885 3031 4177 296
Size: 530 11 1738 310 146 1146 486
Fragments arranged by size:
1738 1146 530 486 310 146 11
EcoICRI GAG'CTC
Cuts at: 842 1379 842
Size: 537 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 580 1054 2192 2624 3371 3639 472
Size: 108 474 1138 432 747 268 1200
Fragments arranged by size:
1200 1138 747 474 432 268 108
EcoO109I rG'GnC_Cy
Cuts at: 231 498 1266 1506 231
Size: 267 768 240 3092
Fragments arranged by size:
3092 768 267 240
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 148 1063 2386 148
Size: 915 1323 2129
Fragments arranged by size:
2129 1323 915
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 979 1286 1801 2049 3071 4050 979
Size: 307 515 248 1022 979 1296
Fragments arranged by size:
1296 1022 979 515 307 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 675 707 811 843 2209 2239 2241 2271 675
Size: 32 104 32 1366 30 2 30 2771
Fragments arranged by size:
2771 1366 104 32 32 30 30 2
HincII GTy'rAC
Cuts at: 396 1406 396
Size: 1010 3357
HindIII A'AGCT_T
Cuts at: 637 637
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3222 3038
Size: 184 4183
MscI TGG'CCA
Cuts at: 331 2128 331
Size: 1797 2570
MslI CAynn'nnrTG
Cuts at: 473 2356 2483 473
Size: 1883 127 2357
Fragments arranged by size:
2357 1883 127
MspA1I CmG'CkG
Cuts at: 450 728 762 876 897 958 1478 1792 2152
Size: 278 34 114 21 61 520 314 360
Cuts at: 2152 3165 3410 450
Size: 1013 245 1407
Fragments arranged by size:
1407 1013 520 360 314 278 245 114
61 34 21
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1420 1920 2827 1420
Size: 500 907 2960
Fragments arranged by size:
2960 907 500
PasI CC'CwG_GG
Cuts at: 747 747
Size: 4367
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PfoI T'CCnGG_A
Cuts at: 911 1031 911
Size: 120 4247
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 1266 1266
Size: 4367
PspOMI G'GGCC_C
Cuts at: 249 249
Size: 4367
PstI C_TGCA'G
Cuts at: 767 826 902 1414 2099 767
Size: 59 76 512 685 3035
Fragments arranged by size:
3035 685 512 76 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 450 728 762 897 958 1792 2152 450
Size: 278 34 135 61 834 360 2665
Fragments arranged by size:
2665 834 360 278 135 61 34
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 844 1381 844
Size: 537 3830
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 1318 2390 2600 1318
Size: 1072 210 3085
Fragments arranged by size:
3085 1072 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 763 822 898 1410 2095 3088 3279 33
Size: 730 59 76 512 685 993 191 1121
Fragments arranged by size:
1121 993 730 685 512 191 76 59
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 502 1061 1522 2437 2929 3191 502
Size: 559 461 915 492 262 1678
Fragments arranged by size:
1678 915 559 492 461 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 509 1501 1855 509
Size: 992 354 3021
Fragments arranged by size:
3021 992 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 770 1950 2083 2542 2751 750
Size: 20 1180 133 459 209 2366
Fragments arranged by size:
2366 1180 459 209 133 20
TatI w'GTAC_w
Cuts at: 197 3882 197
Size: 3685 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1147 1398 1147
Size: 251 4116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 157 454 1862 157
Size: 297 1408 2662
Fragments arranged by size:
2662 1408 297
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 128 1042 128
Size: 914 3453
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI