Restriction map for
pF1KB0499
(Circular) (Six-base) MAPSORT of: pF1KB0499.seq Check: 4656 from: 1 to: 5375
pF1KB0499 5375 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 615 615
Size: 5375
AccI GT'mk_AC
Cuts at: 1492 2413 1492
Size: 921 4454
Acc65I G'GTAC_C
Cuts at: 331 2391 331
Size: 2060 3315
AclI AA'CG_TT
Cuts at: 5215 5215
Size: 5375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1046 2020 3200 3632 4379 4647 1046
Size: 974 1180 432 747 268 1774
Fragments arranged by size:
1774 1180 974 747 432 268
AflIII A'CryG_T
Cuts at: 910 2727 3831 4808 910
Size: 1817 1104 977 1477
Fragments arranged by size:
1817 1477 1104 977
AgeI A'CCGG_T
Cuts at: 3763 3763
Size: 5375
AlwNI CAG_nnn'CTG
Cuts at: 2680 4247 2680
Size: 1567 3808
ApaI G_GGCC'C
Cuts at: 136 136
Size: 5375
ApaLI G'TGCA_C
Cuts at: 596 4145 596
Size: 3549 1826
ApoI r'AATT_y
Cuts at: 421 643 1282 1707 2080 2157 2167 2379 4728
Size: 222 639 425 373 77 10 212 2349
Cuts at: 4728 4984 421
Size: 256 812
Fragments arranged by size:
2349 812 639 425 373 256 222 212
77 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 5375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
AvaI C'yCGr_G
Cuts at: 2395 2395
Size: 5375
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 323 356 323
Size: 33 5342
BamHI G'GATC_C
Cuts at: 2400 2400
Size: 5375
BanI G'GyrC_C
Cuts at: 331 2391 2805 3053 3088 331
Size: 2060 414 248 35 2618
Fragments arranged by size:
2618 2060 414 248 35
BanII G_rGCy'C
Cuts at: 136 2389 3419 136
Size: 2253 1030 2092
Fragments arranged by size:
2253 2092 1030
BbeI G_GCGC'C
Cuts at: 2809 3057 2809
Size: 248 5127
BbsI GAAGACnn'nnnn_
Cuts at: 1526 1997 1526
Size: 471 4904
BciVI GTATCCnnnnn_n'
Cuts at: 3273 4034 5375 3273
Size: 761 1341 3273
Fragments arranged by size:
3273 1341 761
BclI T'GATC_A
Cuts at: 219 2895 219
Size: 2676 2699
BglII A'GATC_T
Cuts at: 2226 2890 4927 2226
Size: 664 2037 2674
Fragments arranged by size:
2674 2037 664
BlpI GC'TnA_GC
Cuts at: 2487 2487
Size: 5375
Bme1580I G_kGCm'C
Cuts at: 136 600 3000 3093 4149 136
Size: 464 2400 93 1056 1362
Fragments arranged by size:
2400 1362 1056 464 93
BmrI ACTGGGnnnn_n'
Cuts at: 1088 1171 2587 2668 3003 5147 1088
Size: 83 1416 81 335 2144 1316
Fragments arranged by size:
2144 1416 1316 335 83 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3742 3742
Size: 5375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1103 2210 1103
Size: 1107 4268
Bpu10I CC'TnA_GC
Cuts at: 2275 2275
Size: 5375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 1969 2551 3430 3922 4220 1937
Size: 32 582 879 492 298 3092
Fragments arranged by size:
3092 879 582 492 298 32
BsaI GGTCTCn'nnnn_
Cuts at: 5079 5079
Size: 5375
BsaAI yAC'GTr
Cuts at: 1197 2728 1197
Size: 1531 3844
BsaBI GATnn'nnATC
Cuts at: 6 1381 2889 4926 6
Size: 1375 1508 2037 455
Fragments arranged by size:
2037 1508 1375 455
BsaHI Gr'CG_yC
Cuts at: 612 2806 3054 3756 5252 612
Size: 2194 248 702 1496 735
Fragments arranged by size:
2194 1496 735 702 248
BsaWI w'CCGG_w
Cuts at: 2564 3085 3763 4037 4184 4595 2564
Size: 521 678 274 147 411 3344
Fragments arranged by size:
3344 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1101 1131 1101
Size: 30 5345
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 158 158
Size: 5375
BseYI C'CCAG_C
Cuts at: 2800 3558 4135 4631 2800
Size: 758 577 496 3544
Fragments arranged by size:
3544 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2917 2963 4171 104
Size: 2813 46 1208 1308
Fragments arranged by size:
2813 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 600 2389 3167 3357 4149 600
Size: 1789 778 190 792 1826
Fragments arranged by size:
1826 1789 792 778 190
BsmI GAATG_Cn'
Cuts at: 550 804 1295 550
Size: 254 491 4630
Fragments arranged by size:
4630 491 254
Bsp1286I G_dGCh'C
Cuts at: 136 600 2389 3000 3093 3167 3357 3419 4149
Size: 464 1789 611 93 74 190 62 730
Cuts at: 4149 136
Size: 1362
Fragments arranged by size:
1789 1362 730 611 464 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4595 4595
Size: 5375
BspHI T'CATG_A
Cuts at: 1055 4551 1055
Size: 3496 1879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1599 2425 2639 2941 3322 1599
Size: 826 214 302 381 3652
Fragments arranged by size:
3652 826 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3667 3721 5205 5370 50
Size: 3617 54 1484 165 55
Fragments arranged by size:
3617 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3287 3287
Size: 5375
BsrFI r'CCGG_y
Cuts at: 3373 3554 3763 3373
Size: 181 209 4985
Fragments arranged by size:
4985 209 181
BssSI C'ACGA_G
Cuts at: 3646 4004 3646
Size: 358 5017
BstAPI GCAn_nnn'nTGC
Cuts at: 2752 2752
Size: 5375
BstBI TT'CG_AA
Cuts at: 3736 3736
Size: 5375
BstYI r'GATC_y
Cuts at: 1 2226 2400 2867 2890 3225 3471 4483 4569
Size: 2225 174 467 23 335 246 1012 86
Cuts at: 4569 4581 4927 1
Size: 12 346 449
Fragments arranged by size:
2225 1012 467 449 346 335 246 174
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1082 1082
Size: 5375
BtgI C'CryG_G
Cuts at: 128 128
Size: 5375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3325 3506 3669 3325
Size: 181 163 5031
Fragments arranged by size:
5031 181 163
BtsI GCAGTG_nn'
Cuts at: 2618 2618
Size: 5375
DraI TTT'AAA
Cuts at: 491 1691 2357 2374 491
Size: 1200 666 17 3492
Fragments arranged by size:
3492 1200 666 17
DrdI GACnn_nn'nnGTC
Cuts at: 3081 3939 3081
Size: 858 4517
EaeI y'GGCC_r
Cuts at: 1775 2960 3134 3525 3552 4897 1775
Size: 1185 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 1185 391 174 27
EagI C'GGCC_G
Cuts at: 2960 2960
Size: 5375
EarI CTCTTCn'nnn_
Cuts at: 462 863 2261 2309 2361 3398 3608 462
Size: 401 1398 48 52 1037 210 2229
Fragments arranged by size:
2229 1398 1037 401 210 52 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3583 3893 4039 5185 3583
Size: 310 146 1146 3773
Fragments arranged by size:
3773 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2387 2387
Size: 5375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1046 1103 2020 2210 3200 3632 4379 4647 1046
Size: 57 917 190 990 432 747 268 1774
Fragments arranged by size:
1774 990 917 747 432 268 190 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 5375
EcoO109I rG'GnC_Cy
Cuts at: 2514 2514
Size: 5375
EcoRI G'AATT_C
Cuts at: 643 2379 643
Size: 1736 3639
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 3394 2338
Size: 1056 4319
FspI TGC'GCA
Cuts at: 3156 3156
Size: 5375
HaeII r_GCGC'y
Cuts at: 1175 2809 3057 4079 5058 1175
Size: 1634 248 1022 979 1492
Fragments arranged by size:
1634 1492 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 700 732 1101 1133 1195 1227 1642 1674 1678
Size: 32 369 32 62 32 415 32 4
Cuts at: 1678 1710 3217 3247 3249 3279 700
Size: 32 1507 30 2 30 2796
Fragments arranged by size:
2796 1507 415 369 62 32 32 32
32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 251 2149 2414 251
Size: 1898 265 3212
Fragments arranged by size:
3212 1898 265
HpaI GTT'AAC
Cuts at: 251 251
Size: 5375
KasI G'GCGC_C
Cuts at: 2805 3053 2805
Size: 248 5127
KpnI G_GTAC'C
Cuts at: 335 2395 335
Size: 2060 3315
MfeI C'AATT_G
Cuts at: 531 617 531
Size: 86 5289
MluI A'CGCG_T
Cuts at: 4808 4808
Size: 5375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 165 4046 4230 165
Size: 3881 184 1310
Fragments arranged by size:
3881 1310 184
MscI TGG'CCA
Cuts at: 1777 3136 1777
Size: 1359 4016
MslI CAynn'nnrTG
Cuts at: 3364 3491 3364
Size: 127 5248
MspA1I CmG'CkG
Cuts at: 130 2486 2800 3160 4173 4418 130
Size: 2356 314 360 1013 245 1087
Fragments arranged by size:
2356 1087 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3556 3556
Size: 5375
NarI GG'CG_CC
Cuts at: 2806 3054 2806
Size: 248 5127
NgoMIV G'CCGG_C
Cuts at: 3554 3554
Size: 5375
NspI r_CATG'y
Cuts at: 455 2308 2428 2928 3835 455
Size: 1853 120 500 907 1995
Fragments arranged by size:
1995 1853 907 500 120
PciI A'CATG_T
Cuts at: 3831 3831
Size: 5375
PflMI CCAn_nnn'nTGG
Cuts at: 721 721
Size: 5375
PfoI T'CCnGG_A
Cuts at: 903 1119 2187 903
Size: 216 1068 4091
Fragments arranged by size:
4091 1068 216
PmeI GTTT'AAAC
Cuts at: 2374 2374
Size: 5375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 5343
PspOMI G'GGCC_C
Cuts at: 132 132
Size: 5375
PstI C_TGCA'G
Cuts at: 1734 2422 3107 1734
Size: 688 685 4002
Fragments arranged by size:
4002 688 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5375
PvuII CAG'CTG
Cuts at: 2800 3160 2800
Size: 360 5015
RsrII CG'GwC_CG
Cuts at: 3570 3570
Size: 5375
SacI G_AGCT'C
Cuts at: 2389 2389
Size: 5375
SacII CC_GC'GG
Cuts at: 131 131
Size: 5375
SalI G'TCGA_C
Cuts at: 2412 2412
Size: 5375
SapI GCTCTTCn'nnn_
Cuts at: 462 3398 3608 462
Size: 2936 210 2229
Fragments arranged by size:
2936 2229 210
SbfI CC_TGCA'GG
Cuts at: 2422 2422
Size: 5375
ScaI AGT'ACT
Cuts at: 4892 4892
Size: 5375
SfcI C'TryA_G
Cuts at: 33 1730 2418 3103 4096 4287 33
Size: 1697 688 685 993 191 1121
Fragments arranged by size:
1697 1121 993 688 685 191
SfoI GGC'GCC
Cuts at: 2807 3055 2807
Size: 248 5127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
SmaI CCC'GGG
Cuts at: 2397 2397
Size: 5375
SmlI C'TyrA_G
Cuts at: 1952 1984 2530 3445 3937 4199 1952
Size: 32 546 915 492 262 3128
Fragments arranged by size:
3128 915 546 492 262 32
SnaBI TAC'GTA
Cuts at: 1197 1197
Size: 5375
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5375
SphI G_CATG'C
Cuts at: 455 2428 2928 455
Size: 1973 500 2902
Fragments arranged by size:
2902 1973 500
SspI AAT'ATT
Cuts at: 634 634
Size: 5375
StyI C'CwwG_G
Cuts at: 1885 2509 2863 1885
Size: 624 354 4397
Fragments arranged by size:
4397 624 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1323 2958 3091 3550 3759 1323
Size: 1635 133 459 209 2939
Fragments arranged by size:
2939 1635 459 209 133
TatI w'GTAC_w
Cuts at: 1096 4890 1096
Size: 3794 1581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 183 215 1031 1063 1962 1994 4892 4924 183
Size: 32 816 32 899 32 2898 32 634
Fragments arranged by size:
2898 899 816 634 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3172 3172
Size: 5375
XbaI T'CTAG_A
Cuts at: 2406 2406
Size: 5375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 2870
Size: 5375
XmaI C'CCGG_G
Cuts at: 2395 2395
Size: 5375
XmnI GAAnn'nnTTC
Cuts at: 1293 1293
Size: 5375
ZraI GAC'GTC
Cuts at: 613 613
Size: 5375
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI