Restriction map for
pF1KB0869
(Circular) (Six-base) MAPSORT of: pF1KB0869.seq Check: 990 from: 1 to: 6335
pF1KB0869 6335 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1600 3147 1600
Size: 1547 4788
AatII G_ACGT'C
Cuts at: 2482 2482
Size: 6335
AccI GT'mk_AC
Cuts at: 1328 3373 1328
Size: 2045 4290
Acc65I G'GTAC_C
Cuts at: 1407 3092 3351 1407
Size: 1685 259 4391
Fragments arranged by size:
4391 1685 259
AclI AA'CG_TT
Cuts at: 6175 6175
Size: 6335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 1579 2239 4160 4592 5339 5607 327
Size: 1252 660 1921 432 747 268 1055
Fragments arranged by size:
1921 1252 1055 747 660 432 268
AfeI AGC'GCT
Cuts at: 620 1352 1966 620
Size: 732 614 4989
Fragments arranged by size:
4989 732 614
AflIII A'CryG_T
Cuts at: 2066 3008 3687 4791 5768 2066
Size: 942 679 1104 977 2633
Fragments arranged by size:
2633 1104 977 942 679
AgeI A'CCGG_T
Cuts at: 485 4723 485
Size: 4238 2097
AleI CACnn'nnGTG
Cuts at: 1036 1736 1036
Size: 700 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1453 2629 1453
Size: 1176 5159
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 195 227 2649 2681 195
Size: 32 2422 32 3849
Fragments arranged by size:
3849 2422 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1197 1500 1592 2144 2393 3640 5207 1197
Size: 303 92 552 249 1247 1567 2325
Fragments arranged by size:
2325 1567 1247 552 303 249 92
ApaI G_GGCC'C
Cuts at: 1109 1605 1861 1109
Size: 496 256 5583
Fragments arranged by size:
5583 496 256
ApaLI G'TGCA_C
Cuts at: 5105 5105
Size: 6335
ApoI r'AATT_y
Cuts at: 786 2328 3339 5688 5944 786
Size: 1542 1011 2349 256 1177
Fragments arranged by size:
2349 1542 1177 1011 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6335
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6335
AvaI C'yCGr_G
Cuts at: 860 3355 860
Size: 2495 3840
AvrII C'CTAG_G
Cuts at: 699 699
Size: 6335
BamHI G'GATC_C
Cuts at: 1789 3360 1789
Size: 1571 4764
BanI G'GyrC_C
Cuts at: 1180 1407 1565 2383 3092 3351 3765 4013 4048
Size: 227 158 818 709 259 414 248 35
Cuts at: 4048 1180
Size: 3467
Fragments arranged by size:
3467 818 709 414 259 248 227 158
35
BanII G_rGCy'C
Cuts at: 644 1109 1457 1481 1605 1861 3349 4379 644
Size: 465 348 24 124 256 1488 1030 2600
Fragments arranged by size:
2600 1488 1030 465 348 256 124 24
BbeI G_GCGC'C
Cuts at: 3769 4017 3769
Size: 248 6087
BbsI GAAGACnn'nnnn_
Cuts at: 908 1062 2230 2352 3286 908
Size: 154 1168 122 934 3957
Fragments arranged by size:
3957 1168 934 154 122
BbvCI CC'TCA_GC
Cuts at: 1056 1056
Size: 6335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1854 1888 2285 2319 1854
Size: 34 397 34 5870
Fragments arranged by size:
5870 397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1160 4233 4994 6335 1160
Size: 3073 761 1341 1160
Fragments arranged by size:
3073 1341 1160 761
BclI T'GATC_A
Cuts at: 564 3855 564
Size: 3291 3044
BfrBI ATG'CAT
Cuts at: 511 1254 511
Size: 743 5592
BglI GCCn_nnn'nGGC
Cuts at: 2317 3211 2317
Size: 894 5441
BglII A'GATC_T
Cuts at: 3306 3850 5887 3306
Size: 544 2037 3754
Fragments arranged by size:
3754 2037 544
BlpI GC'TnA_GC
Cuts at: 3034 3447 3034
Size: 413 5922
Bme1580I G_kGCm'C
Cuts at: 1109 1286 1605 1861 3960 4053 5109 1109
Size: 177 319 256 2099 93 1056 2335
Fragments arranged by size:
2335 2099 1056 319 256 177 93
BmgBI CAC'GTC
Cuts at: 676 1332 676
Size: 656 5679
BmrI ACTGGGnnnn_n'
Cuts at: 2617 3284 3547 3628 3963 6107 2617
Size: 667 263 81 335 2144 2845
Fragments arranged by size:
2845 2144 667 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4702 4702
Size: 6335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 834 1675 834
Size: 841 5494
Bpu10I CC'TnA_GC
Cuts at: 1001 1056 1078 1666 2213 1001
Size: 55 22 588 547 5123
Fragments arranged by size:
5123 588 547 55 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 376 1410 3511 4390 4882 5180 126
Size: 250 1034 2101 879 492 298 1281
Fragments arranged by size:
2101 1281 1034 879 492 298 250
BsaI GGTCTCn'nnnn_
Cuts at: 1751 1925 6039 1751
Size: 174 4114 2047
Fragments arranged by size:
4114 2047 174
BsaAI yAC'GTr
Cuts at: 1203 1320 1542 3688 1203
Size: 117 222 2146 3850
Fragments arranged by size:
3850 2146 222 117
BsaBI GATnn'nnATC
Cuts at: 6 3849 5886 6
Size: 3843 2037 455
Fragments arranged by size:
3843 2037 455
BsaHI Gr'CG_yC
Cuts at: 1756 2479 2843 3766 4014 4716 6212 1756
Size: 723 364 923 248 702 1496 1879
Fragments arranged by size:
1879 1496 923 723 702 364 248
BsaWI w'CCGG_w
Cuts at: 485 2525 3524 4045 4723 4997 5144 5555 485
Size: 2040 999 521 678 274 147 411 1265
Fragments arranged by size:
2040 1265 999 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 701 731 2173 2203 701
Size: 30 1442 30 4833
Fragments arranged by size:
4833 1442 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 704 1672 3049 613
Size: 91 968 1377 3899
Fragments arranged by size:
3899 1377 968 91
BseYI C'CCAG_C
Cuts at: 602 1622 2081 2431 3760 4518 5095 5591 602
Size: 1020 459 350 1329 758 577 496 1346
Fragments arranged by size:
1346 1329 1020 758 577 496 459 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 1357 1486 2186 2514 2740 510
Size: 847 129 700 328 226 4105
Fragments arranged by size:
4105 847 700 328 226 129
BsiEI CG_ry'CG
Cuts at: 104 486 2923 3877 3923 5131 104
Size: 382 2437 954 46 1208 1308
Fragments arranged by size:
2437 1308 1208 954 382 46
BsiHKAI G_wGCw'C
Cuts at: 644 1457 1541 2899 3349 4127 4317 5109 644
Size: 813 84 1358 450 778 190 792 1870
Fragments arranged by size:
1870 1358 813 792 778 450 190 84
BsmI GAATG_Cn'
Cuts at: 273 511 2123 2516 2573 2608 273
Size: 238 1612 393 57 35 4000
Fragments arranged by size:
4000 1612 393 238 57 35
BsmBI CGTCTCn'nnnn_
Cuts at: 3138 3138
Size: 6335
Bsp1286I G_dGCh'C
Cuts at: 644 1109 1286 1457 1481 1541 1605 1861 2899
Size: 465 177 171 24 60 64 256 1038
Cuts at: 2899 3349 3960 4053 4127 4317 4379 5109 644
Size: 450 611 93 74 190 62 730 1870
Fragments arranged by size:
1870 1038 730 611 465 450 256 190
177 171 93 74 64 62 60 24
BspEI T'CCGG_A
Cuts at: 5555 5555
Size: 6335
BspHI T'CATG_A
Cuts at: 920 5511 920
Size: 4591 1744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1600 3147 3385 3599 3901 4282 1600
Size: 1547 238 214 302 381 3653
Fragments arranged by size:
3653 1547 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 536 1811 2705 4627 4681 6165 6330 50
Size: 486 1275 894 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 1275 894 486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 2046 2181 4247 1046
Size: 1000 135 2066 3134
Fragments arranged by size:
3134 2066 1000 135
BsrFI r'CCGG_y
Cuts at: 485 2918 4333 4514 4723 485
Size: 2433 1415 181 209 2097
Fragments arranged by size:
2433 2097 1415 209 181
BssSI C'ACGA_G
Cuts at: 1914 4606 4964 1914
Size: 2692 358 3285
Fragments arranged by size:
3285 2692 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1500 1592 2894 3712 1500
Size: 92 1302 818 4123
Fragments arranged by size:
4123 1302 818 92
BstBI TT'CG_AA
Cuts at: 4696 4696
Size: 6335
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 6335
BstYI r'GATC_y
Cuts at: 1 360 557 1496 1550 1789 2141 2968 3306
Size: 359 197 939 54 239 352 827 338
Cuts at: 3306 3360 3827 3850 4185 4431 5443 5529 5541
Size: 54 467 23 335 246 1012 86 12
Cuts at: 5541 5887 1
Size: 346 449
Fragments arranged by size:
1012 939 827 467 449 359 352 346
338 335 246 239 197 86 54 54
23 12
BtgI C'CryG_G
Cuts at: 659 1184 1721 1750 1784 2882 2909 3026 659
Size: 525 537 29 34 1098 27 117 3968
Fragments arranged by size:
3968 1098 537 525 117 34 29 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1071 4285 4466 4629 1071
Size: 3214 181 163 2777
Fragments arranged by size:
3214 2777 181 163
BtsI GCAGTG_nn'
Cuts at: 1684 1840 1855 3578 1684
Size: 156 15 1723 4441
Fragments arranged by size:
4441 1723 156 15
DraI TTT'AAA
Cuts at: 3334 3334
Size: 6335
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2484 4041 4899 1917
Size: 567 1557 858 3353
Fragments arranged by size:
3353 1557 858 567
EaeI y'GGCC_r
Cuts at: 1373 2274 2920 3920 4094 4485 4512 5857 1373
Size: 901 646 1000 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 1000 901 646 391 174 27
EagI C'GGCC_G
Cuts at: 2920 3920 2920
Size: 1000 5335
EarI CTCTTCn'nnn_
Cuts at: 346 1439 1462 1555 2759 2978 4358 4568 346
Size: 1093 23 93 1204 219 1380 210 2113
Fragments arranged by size:
2113 1380 1204 1093 219 210 93 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1485 4543 4853 4999 6145 1485
Size: 3058 310 146 1146 1675
Fragments arranged by size:
3058 1675 1146 310 146
EcoICRI GAG'CTC
Cuts at: 642 1455 3347 642
Size: 813 1892 3630
Fragments arranged by size:
3630 1892 813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 834 1579 1675 2239 4160 4592 5339 5607
Size: 507 745 96 564 1921 432 747 268
Cuts at: 5607 327
Size: 1055
Fragments arranged by size:
1921 1055 747 745 564 507 432 268
96
EcoO109I rG'GnC_Cy
Cuts at: 1106 1194 1601 1608 1857 1858 2086 2699 3474
Size: 88 407 7 249 1 228 613 775
Cuts at: 3474 1106
Size: 3967
Fragments arranged by size:
3967 775 613 407 249 228 88 7
1
EcoRI G'AATT_C
Cuts at: 3339 3339
Size: 6335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4354 4354
Size: 6335
FspI TGC'GCA
Cuts at: 4116 4116
Size: 6335
HaeII r_GCGC'y
Cuts at: 622 891 1085 1354 1784 1968 3769 4017 5039
Size: 269 194 269 430 184 1801 248 1022
Cuts at: 5039 6018 622
Size: 979 939
Fragments arranged by size:
1801 1022 979 939 430 269 269 248
194 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 549 581 1698 1730 1781 1813 2173
Size: 32 168 32 1117 32 51 32 360
Cuts at: 2173 2205 2339 2371 2726 2758 2788 2820 4177
Size: 32 134 32 355 32 30 32 1357
Cuts at: 4177 4207 4209 4239 349
Size: 30 2 30 2445
Fragments arranged by size:
2445 1357 1117 360 355 168 134 51
32 32 32 32 32 32 32 32
30 30 30 2
HincII GTy'rAC
Cuts at: 290 1329 2256 2457 3374 290
Size: 1039 927 201 917 3251
Fragments arranged by size:
3251 1039 927 917 201
HpaI GTT'AAC
Cuts at: 290 290
Size: 6335
KasI G'GCGC_C
Cuts at: 3765 4013 3765
Size: 248 6087
KpnI G_GTAC'C
Cuts at: 1411 3096 3355 1411
Size: 1685 259 4391
Fragments arranged by size:
4391 1685 259
MluI A'CGCG_T
Cuts at: 5768 5768
Size: 6335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2786 2795 5006 5190 1729
Size: 1057 9 2211 184 2874
Fragments arranged by size:
2874 2211 1057 184 9
MscI TGG'CCA
Cuts at: 1375 4096 1375
Size: 2721 3614
MslI CAynn'nnrTG
Cuts at: 463 830 919 1036 1249 1736 1772 4324 4451
Size: 367 89 117 213 487 36 2552 127
Cuts at: 4451 463
Size: 2347
Fragments arranged by size:
2552 2347 487 367 213 127 117 89
36
MspA1I CmG'CkG
Cuts at: 587 631 1060 1433 3090 3446 3760 4120 5133
Size: 44 429 373 1657 356 314 360 1013
Cuts at: 5133 5378 587
Size: 245 1544
Fragments arranged by size:
1657 1544 1013 429 373 360 356 314
245 44
NaeI GCC'GGC
Cuts at: 2920 4516 2920
Size: 1596 4739
NarI GG'CG_CC
Cuts at: 3766 4014 3766
Size: 248 6087
NcoI C'CATG_G
Cuts at: 659 2882 659
Size: 2223 4112
NgoMIV G'CCGG_C
Cuts at: 2918 4514 2918
Size: 1596 4739
NsiI A_TGCA'T
Cuts at: 513 1256 513
Size: 743 5592
NspI r_CATG'y
Cuts at: 2070 2306 2678 3012 3388 3888 4795 2070
Size: 236 372 334 376 500 907 3610
Fragments arranged by size:
3610 907 500 376 372 334 236
PasI CC'CwG_GG
Cuts at: 180 180
Size: 6335
PciI A'CATG_T
Cuts at: 2066 3008 4791 2066
Size: 942 1783 3610
Fragments arranged by size:
3610 1783 942
PflMI CCAn_nnn'nTGG
Cuts at: 381 2234 2629 381
Size: 1853 395 4087
Fragments arranged by size:
4087 1853 395
PfoI T'CCnGG_A
Cuts at: 669 1885 2899 669
Size: 1216 1014 4105
Fragments arranged by size:
4105 1216 1014
PmeI GTTT'AAAC
Cuts at: 3334 3334
Size: 6335
PmlI CAC'GTG
Cuts at: 1320 1542 1320
Size: 222 6113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 5474
Size: 32 6303
PpuMI rG'GwC_Cy
Cuts at: 1608 2086 2699 1608
Size: 478 613 5244
Fragments arranged by size:
5244 613 478
PshAI GACnn'nnGTC
Cuts at: 2251 2251
Size: 6335
PspOMI G'GGCC_C
Cuts at: 1105 1601 1857 1105
Size: 496 256 5583
Fragments arranged by size:
5583 496 256
PstI C_TGCA'G
Cuts at: 1229 1496 1930 1975 2509 3382 4067 1229
Size: 267 434 45 534 873 685 3497
Fragments arranged by size:
3497 873 685 534 434 267 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6335
PvuII CAG'CTG
Cuts at: 631 1060 1433 3760 4120 631
Size: 429 373 2327 360 2846
Fragments arranged by size:
2846 2327 429 373 360
RsrII CG'GwC_CG
Cuts at: 4530 4530
Size: 6335
SacI G_AGCT'C
Cuts at: 644 1457 3349 644
Size: 813 1892 3630
Fragments arranged by size:
3630 1892 813
SalI G'TCGA_C
Cuts at: 1327 3372 1327
Size: 2045 4290
SapI GCTCTTCn'nnn_
Cuts at: 1462 4358 4568 1462
Size: 2896 210 3229
Fragments arranged by size:
3229 2896 210
SbfI CC_TGCA'GG
Cuts at: 3382 3382
Size: 6335
ScaI AGT'ACT
Cuts at: 987 5852 987
Size: 4865 1470
SfcI C'TryA_G
Cuts at: 33 594 653 1225 1492 1926 1971 2505 3378
Size: 561 59 572 267 434 45 534 873
Cuts at: 3378 4063 5056 5247 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 873 685 572 561 534 434
267 191 59 45
SfoI GGC'GCC
Cuts at: 3767 4015 3767
Size: 248 6087
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6335
SmaI CCC'GGG
Cuts at: 862 3357 862
Size: 2495 3840
SmlI C'TyrA_G
Cuts at: 141 355 1425 3490 4405 4897 5159 141
Size: 214 1070 2065 915 492 262 1317
Fragments arranged by size:
2065 1317 1070 915 492 262 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6335
SphI G_CATG'C
Cuts at: 3388 3888 3388
Size: 500 5835
StuI AGG'CCT
Cuts at: 415 1096 415
Size: 681 5654
StyI C'CwwG_G
Cuts at: 659 699 2089 2577 2882 3469 3823 659
Size: 40 1390 488 305 587 354 3171
Fragments arranged by size:
3171 1390 587 488 354 305 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 185 466 1228 3918 4051 4510 4719 185
Size: 281 762 2690 133 459 209 1801
Fragments arranged by size:
2690 1801 762 459 281 209 133
TatI w'GTAC_w
Cuts at: 731 985 5850 731
Size: 254 4865 1216
Fragments arranged by size:
4865 1216 254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 2620 2652 5852 5884 376
Size: 32 2212 32 3200 32 827
Fragments arranged by size:
3200 2212 827 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4132 4132
Size: 6335
XbaI T'CTAG_A
Cuts at: 3366 3366
Size: 6335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1174 2069 2116 3830 1174
Size: 895 47 1714 3679
Fragments arranged by size:
3679 1714 895 47
XmaI C'CCGG_G
Cuts at: 860 3355 860
Size: 2495 3840
ZraI GAC'GTC
Cuts at: 2480 2480
Size: 6335
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI BaeI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI