Restriction map for
pF1KB0901
(Circular) (Six-base) MAPSORT of: pF1KB0901.seq Check: 6591 from: 1 to: 3353
pF1KB0901 3353 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 391 391
Size: 3353
Acc65I G'GTAC_C
Cuts at: 369 369
Size: 3353
AclI AA'CG_TT
Cuts at: 3193 3193
Size: 3353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 1178 1610 2357 2625 308
Size: 870 432 747 268 1036
Fragments arranged by size:
1036 870 747 432 268
AflIII A'CryG_T
Cuts at: 705 1809 2786 705
Size: 1104 977 1272
Fragments arranged by size:
1272 1104 977
AgeI A'CCGG_T
Cuts at: 1741 1741
Size: 3353
AhdI GACnn_n'nnGTC
Cuts at: 276 276
Size: 3353
AlwNI CAG_nnn'CTG
Cuts at: 331 658 2225 331
Size: 327 1567 1459
Fragments arranged by size:
1567 1459 327
ApaLI G'TGCA_C
Cuts at: 2123 2123
Size: 3353
ApoI r'AATT_y
Cuts at: 357 2706 2962 357
Size: 2349 256 748
Fragments arranged by size:
2349 748 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3353
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3353
AvaI C'yCGr_G
Cuts at: 373 373
Size: 3353
BamHI G'GATC_C
Cuts at: 378 378
Size: 3353
BanI G'GyrC_C
Cuts at: 369 783 1031 1066 369
Size: 414 248 35 2656
Fragments arranged by size:
2656 414 248 35
BanII G_rGCy'C
Cuts at: 289 367 1397 289
Size: 78 1030 2245
Fragments arranged by size:
2245 1030 78
BbeI G_GCGC'C
Cuts at: 787 1035 787
Size: 248 3105
BciVI GTATCCnnnnn_n'
Cuts at: 238 1251 2012 3353 238
Size: 1013 761 1341 238
Fragments arranged by size:
1341 1013 761 238
BclI T'GATC_A
Cuts at: 873 873
Size: 3353
BglII A'GATC_T
Cuts at: 868 2905 868
Size: 2037 1316
BlpI GC'TnA_GC
Cuts at: 465 465
Size: 3353
Bme1580I G_kGCm'C
Cuts at: 978 1071 2127 978
Size: 93 1056 2204
Fragments arranged by size:
2204 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 565 646 981 3125 565
Size: 81 335 2144 793
Fragments arranged by size:
2144 793 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 336 1720 336
Size: 1384 1969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 258
Size: 3353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1408 1900 2198 529
Size: 879 492 298 1684
Fragments arranged by size:
1684 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3057 3057
Size: 3353
BsaAI yAC'GTr
Cuts at: 706 706
Size: 3353
BsaBI GATnn'nnATC
Cuts at: 6 867 2904 6
Size: 861 2037 455
Fragments arranged by size:
2037 861 455
BsaHI Gr'CG_yC
Cuts at: 784 1032 1734 3230 784
Size: 248 702 1496 907
Fragments arranged by size:
1496 907 702 248
BsaWI w'CCGG_w
Cuts at: 542 1063 1741 2015 2162 2573 542
Size: 521 678 274 147 411 1322
Fragments arranged by size:
1322 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 123 153 123
Size: 30 3323
BseYI C'CCAG_C
Cuts at: 222 778 1536 2113 2609 222
Size: 556 758 577 496 966
Fragments arranged by size:
966 758 577 556 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 279 202
Size: 77 3276
BsiEI CG_ry'CG
Cuts at: 104 895 941 2149 104
Size: 791 46 1208 1308
Fragments arranged by size:
1308 1208 791 46
BsiHKAI G_wGCw'C
Cuts at: 367 1145 1335 2127 367
Size: 778 190 792 1593
Fragments arranged by size:
1593 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 289 367 978 1071 1145 1335 1397 2127 289
Size: 78 611 93 74 190 62 730 1515
Fragments arranged by size:
1515 730 611 190 93 78 74 62
BspEI T'CCGG_A
Cuts at: 2573 2573
Size: 3353
BspHI T'CATG_A
Cuts at: 2529 2529
Size: 3353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 301 403 617 919 1300 301
Size: 102 214 302 381 2354
Fragments arranged by size:
2354 381 302 214 102
BsrBI CCG'CTC
Cuts at: 50 1645 1699 3183 3348 50
Size: 1595 54 1484 165 55
Fragments arranged by size:
1595 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1265 1265
Size: 3353
BsrFI r'CCGG_y
Cuts at: 1351 1532 1741 1351
Size: 181 209 2963
Fragments arranged by size:
2963 209 181
BssSI C'ACGA_G
Cuts at: 1624 1982 1624
Size: 358 2995
BstAPI GCAn_nnn'nTGC
Cuts at: 730 730
Size: 3353
BstBI TT'CG_AA
Cuts at: 1714 1714
Size: 3353
BstYI r'GATC_y
Cuts at: 1 378 845 868 1203 1449 2461 2547 2559
Size: 377 467 23 335 246 1012 86 12
Cuts at: 2559 2905 1
Size: 346 449
Fragments arranged by size:
1012 467 449 377 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 143 143
Size: 3353
BtgI C'CryG_G
Cuts at: 107 107
Size: 3353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1303 1484 1647 1303
Size: 181 163 3009
Fragments arranged by size:
3009 181 163
BtsI GCAGTG_nn'
Cuts at: 596 596
Size: 3353
DraI TTT'AAA
Cuts at: 352 352
Size: 3353
DrdI GACnn_nn'nnGTC
Cuts at: 1059 1917 1059
Size: 858 2495
EaeI y'GGCC_r
Cuts at: 938 1112 1503 1530 2875 938
Size: 174 391 27 1345 1416
Fragments arranged by size:
1416 1345 391 174 27
EagI C'GGCC_G
Cuts at: 938 938
Size: 3353
EarI CTCTTCn'nnn_
Cuts at: 327 1376 1586 327
Size: 1049 210 2094
Fragments arranged by size:
2094 1049 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 283 1561 1871 2017 3163 283
Size: 1278 310 146 1146 473
Fragments arranged by size:
1278 1146 473 310 146
EcoICRI GAG'CTC
Cuts at: 365 365
Size: 3353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 308 1178 1610 2357 2625 258
Size: 50 870 432 747 268 986
Fragments arranged by size:
986 870 747 432 268 50
EcoO109I rG'GnC_Cy
Cuts at: 492 492
Size: 3353
EcoRI G'AATT_C
Cuts at: 357 357
Size: 3353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1372 1372
Size: 3353
FspI TGC'GCA
Cuts at: 1134 1134
Size: 3353
HaeII r_GCGC'y
Cuts at: 787 1035 2057 3036 787
Size: 248 1022 979 1104
Fragments arranged by size:
1104 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 1195 1225 1227 1257 262
Size: 32 901 30 2 30 2358
Fragments arranged by size:
2358 901 32 30 30 2
HincII GTy'rAC
Cuts at: 392 392
Size: 3353
KasI G'GCGC_C
Cuts at: 783 1031 783
Size: 248 3105
KpnI G_GTAC'C
Cuts at: 373 373
Size: 3353
MluI A'CGCG_T
Cuts at: 2786 2786
Size: 3353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2024 2208 2024
Size: 184 3169
MscI TGG'CCA
Cuts at: 1114 1114
Size: 3353
MslI CAynn'nnrTG
Cuts at: 1342 1469 1342
Size: 127 3226
MspA1I CmG'CkG
Cuts at: 464 778 1138 2151 2396 464
Size: 314 360 1013 245 1421
Fragments arranged by size:
1421 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1534 1534
Size: 3353
NarI GG'CG_CC
Cuts at: 784 1032 784
Size: 248 3105
NcoI C'CATG_G
Cuts at: 107 107
Size: 3353
NgoMIV G'CCGG_C
Cuts at: 1532 1532
Size: 3353
NspI r_CATG'y
Cuts at: 406 906 1813 406
Size: 500 907 1946
Fragments arranged by size:
1946 907 500
PciI A'CATG_T
Cuts at: 1809 1809
Size: 3353
PmeI GTTT'AAAC
Cuts at: 352 352
Size: 3353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2492 2524 2492
Size: 32 3321
PstI C_TGCA'G
Cuts at: 400 1085 400
Size: 685 2668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3353
PvuII CAG'CTG
Cuts at: 778 1138 778
Size: 360 2993
RsrII CG'GwC_CG
Cuts at: 1548 1548
Size: 3353
SacI G_AGCT'C
Cuts at: 367 367
Size: 3353
SalI G'TCGA_C
Cuts at: 390 390
Size: 3353
SapI GCTCTTCn'nnn_
Cuts at: 1376 1586 1376
Size: 210 3143
SbfI CC_TGCA'GG
Cuts at: 400 400
Size: 3353
ScaI AGT'ACT
Cuts at: 2870 2870
Size: 3353
SfcI C'TryA_G
Cuts at: 33 396 1081 2074 2265 33
Size: 363 685 993 191 1121
Fragments arranged by size:
1121 993 685 363 191
SfoI GGC'GCC
Cuts at: 785 1033 785
Size: 248 3105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3353
SmaI CCC'GGG
Cuts at: 375 375
Size: 3353
SmlI C'TyrA_G
Cuts at: 508 1423 1915 2177 508
Size: 915 492 262 1684
Fragments arranged by size:
1684 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3353
SphI G_CATG'C
Cuts at: 406 906 406
Size: 500 2853
StyI C'CwwG_G
Cuts at: 107 487 841 107
Size: 380 354 2619
Fragments arranged by size:
2619 380 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 936 1069 1528 1737 936
Size: 133 459 209 2552
Fragments arranged by size:
2552 459 209 133
TatI w'GTAC_w
Cuts at: 2868 2868
Size: 3353
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 2870 2902 123
Size: 32 2715 32 574
Fragments arranged by size:
2715 574 32 32
Tth111I GACn'n_nGTC
Cuts at: 1150 1150
Size: 3353
XbaI T'CTAG_A
Cuts at: 384 384
Size: 3353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 848 848
Size: 3353
XmaI C'CCGG_G
Cuts at: 373 373
Size: 3353
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI