Restriction map for
pF1KB0904
(Circular) (Six-base) MAPSORT of: pF1KB0904.seq Check: 3267 from: 1 to: 4010
pF1KB0904 4010 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 300 615 1835 2267 3014 3282 196
Size: 104 315 1220 432 747 268 924
Fragments arranged by size:
1220 924 747 432 315 268 104
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 694 1315 2882 694
Size: 621 1567 1822
Fragments arranged by size:
1822 1567 621
ApaI G_GGCC'C
Cuts at: 155 582 738 155
Size: 427 156 3427
Fragments arranged by size:
3427 427 156
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 155 1030 155
Size: 875 3135
AvrII C'CTAG_G
Cuts at: 477 477
Size: 4010
BamHI G'GATC_C
Cuts at: 320 1035 320
Size: 715 3295
BanI G'GyrC_C
Cuts at: 196 1026 1440 1688 1723 196
Size: 830 414 248 35 2483
Fragments arranged by size:
2483 830 414 248 35
BanII G_rGCy'C
Cuts at: 155 582 738 1024 2054 155
Size: 427 156 286 1030 2111
Fragments arranged by size:
2111 1030 427 286 156
BbeI G_GCGC'C
Cuts at: 200 1444 1692 200
Size: 1244 248 2518
Fragments arranged by size:
2518 1244 248
BciVI GTATCCnnnnn_n'
Cuts at: 597 1908 2669 4010 597
Size: 1311 761 1341 597
Fragments arranged by size:
1341 1311 761 597
BclI T'GATC_A
Cuts at: 566 1530 566
Size: 964 3046
BglI GCCn_nnn'nGGC
Cuts at: 150 733 150
Size: 583 3427
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 667 1122 667
Size: 455 3555
Bme1580I G_kGCm'C
Cuts at: 155 169 248 582 738 1635 1728 2784 155
Size: 14 79 334 156 897 93 1056 1381
Fragments arranged by size:
1381 1056 897 334 156 93 79 14
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
Bpu10I CC'TnA_GC
Cuts at: 449 449
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1186 2065 2557 2855 514
Size: 672 879 492 298 1669
Fragments arranged by size:
1669 879 672 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 197 1441 1689 2391 3887 197
Size: 1244 248 702 1496 320
Fragments arranged by size:
1496 1244 702 320 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 369 369
Size: 4010
BseYI C'CCAG_C
Cuts at: 186 572 716 728 1435 2193 2770 3266 186
Size: 386 144 12 707 758 577 496 930
Fragments arranged by size:
930 758 707 577 496 386 144 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 805 301
Size: 504 3506
BsiEI CG_ry'CG
Cuts at: 104 899 1552 1598 2806 104
Size: 795 653 46 1208 1308
Fragments arranged by size:
1308 1208 795 653 46
BsiHKAI G_wGCw'C
Cuts at: 530 1024 1802 1992 2784 530
Size: 494 778 190 792 1756
Fragments arranged by size:
1756 792 778 494 190
Bsp1286I G_dGCh'C
Cuts at: 155 169 248 530 582 738 1024 1635 1728
Size: 14 79 282 52 156 286 611 93
Cuts at: 1728 1802 1992 2054 2784 155
Size: 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 286 282 190 156 93
79 74 62 52 14
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 193 2008 2189 2398 193
Size: 1815 181 209 1805
Fragments arranged by size:
1815 1805 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstXI CCAn_nnnn'nTGG
Cuts at: 711 711
Size: 4010
BstYI r'GATC_y
Cuts at: 1 320 1035 1502 1525 1860 2106 3118 3204
Size: 319 715 467 23 335 246 1012 86
Cuts at: 3204 3216 3562 1
Size: 12 346 449
Fragments arranged by size:
1012 715 467 449 346 335 319 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 200 233 107
Size: 93 33 3884
Fragments arranged by size:
3884 93 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 1253 1253
Size: 4010
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 299 542 653 1595 1769 2160 2187 3532 299
Size: 243 111 942 174 391 27 1345 777
Fragments arranged by size:
1345 942 777 391 243 174 111 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 589 2218 2528 2674 3820 589
Size: 1629 310 146 1146 779
Fragments arranged by size:
1629 1146 779 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 300 615 1835 2267 3014 3282 196
Size: 104 315 1220 432 747 268 924
Fragments arranged by size:
1220 924 747 432 315 268 104
EcoNI CCTnn'n_nnAGG
Cuts at: 301 991 301
Size: 690 3320
EcoO109I rG'GnC_Cy
Cuts at: 152 306 1149 152
Size: 154 843 3013
Fragments arranged by size:
3013 843 154
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 200 477 1444 1692 2714 3693 200
Size: 277 967 248 1022 979 517
Fragments arranged by size:
1022 979 967 517 277 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1852 1882 1884 1914 1852
Size: 30 2 30 3948
Fragments arranged by size:
3948 30 30 2
HincII GTy'rAC
Cuts at: 519 1049 519
Size: 530 3480
KasI G'GCGC_C
Cuts at: 196 1440 1688 196
Size: 1244 248 2518
Fragments arranged by size:
2518 1244 248
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 544 655 1771 544
Size: 111 1116 2783
Fragments arranged by size:
2783 1116 111
MslI CAynn'nnrTG
Cuts at: 1999 2126 1999
Size: 127 3883
MspA1I CmG'CkG
Cuts at: 564 576 691 732 771 931 1121 1435 1795
Size: 12 115 41 39 160 190 314 360
Cuts at: 1795 2808 3053 564
Size: 1013 245 1521
Fragments arranged by size:
1521 1013 360 314 245 190 160 115
41 39 12
NaeI GCC'GGC
Cuts at: 195 2191 195
Size: 1996 2014
NarI GG'CG_CC
Cuts at: 197 1441 1689 197
Size: 1244 248 2518
Fragments arranged by size:
2518 1244 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 193 2189 193
Size: 1996 2014
NspI r_CATG'y
Cuts at: 468 1063 1563 2470 468
Size: 595 500 907 2008
Fragments arranged by size:
2008 907 595 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 4010
PfoI T'CCnGG_A
Cuts at: 641 641
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PspOMI G'GGCC_C
Cuts at: 151 578 734 151
Size: 427 156 3427
Fragments arranged by size:
3427 427 156
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 832 864 832
Size: 32 3978
PstI C_TGCA'G
Cuts at: 1057 1742 1057
Size: 685 3325
PvuI CG_AT'CG
Cuts at: 104 899 104
Size: 795 3215
PvuII CAG'CTG
Cuts at: 564 691 931 1435 1795 564
Size: 127 240 504 360 2779
Fragments arranged by size:
2779 504 360 240 127
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 386 403 695 952 1053 1738 2731 2922
Size: 353 17 292 257 101 685 993 191
Cuts at: 2922 33
Size: 1121
Fragments arranged by size:
1121 993 685 353 292 257 191 101
17
SfiI GGCCn_nnn'nGGCC
Cuts at: 733 733
Size: 4010
SfoI GGC'GCC
Cuts at: 198 1442 1690 198
Size: 1244 248 2518
Fragments arranged by size:
2518 1244 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 157 1032 157
Size: 875 3135
SmlI C'TyrA_G
Cuts at: 529 1165 2080 2572 2834 529
Size: 636 915 492 262 1705
Fragments arranged by size:
1705 915 636 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 107 325 477 623 1144 1498 107
Size: 218 152 146 521 354 2619
Fragments arranged by size:
2619 521 354 218 152 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 586 1593 1726 2185 2394 586
Size: 1007 133 459 209 2202
Fragments arranged by size:
2202 1007 459 209 133
TatI w'GTAC_w
Cuts at: 683 3525 683
Size: 2842 1168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 155 1030 155
Size: 875 3135
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspXI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI