Restriction map for
pF1KB0906
(Circular) (Six-base) MAPSORT of: pF1KB0906.seq Check: 8646 from: 1 to: 4028
pF1KB0906 4028 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 679 1853 2285 3032 3300 412
Size: 267 1174 432 747 268 1140
Fragments arranged by size:
1174 1140 747 432 268 267
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 300 384 745 1333 2900 300
Size: 84 361 588 1567 1428
Fragments arranged by size:
1567 1428 588 361 84
ApaLI G'TGCA_C
Cuts at: 247 2798 247
Size: 2551 1477
ApoI r'AATT_y
Cuts at: 589 1032 3381 3637 589
Size: 443 2349 256 980
Fragments arranged by size:
2349 980 443 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 151 197 1048 151
Size: 46 851 3131
Fragments arranged by size:
3131 851 46
BamHI G'GATC_C
Cuts at: 374 974 1053 374
Size: 600 79 3349
Fragments arranged by size:
3349 600 79
BanI G'GyrC_C
Cuts at: 201 334 532 1044 1458 1706 1741 201
Size: 133 198 512 414 248 35 2488
Fragments arranged by size:
2488 512 414 248 198 133 35
BanII G_rGCy'C
Cuts at: 152 658 1042 2072 152
Size: 506 384 1030 2108
Fragments arranged by size:
2108 1030 506 384
BbeI G_GCGC'C
Cuts at: 205 1462 1710 205
Size: 1257 248 2523
Fragments arranged by size:
2523 1257 248
BbvCI CC'TCA_GC
Cuts at: 638 638
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 251 337 1653 1746 2802 251
Size: 86 1316 93 1056 1477
Fragments arranged by size:
1477 1316 1056 93 86
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 292 490 530 241
Size: 51 198 40 3739
Fragments arranged by size:
3739 198 51 40
Bpu10I CC'TnA_GC
Cuts at: 638 638
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 2575 2873 1204
Size: 879 492 298 2359
Fragments arranged by size:
2359 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 268 3732 268
Size: 3464 564
BsaAI yAC'GTr
Cuts at: 597 1381 597
Size: 784 3244
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 202 1459 1707 2409 3905 202
Size: 1257 248 702 1496 325
Fragments arranged by size:
1496 1257 702 325 248
BsaWI w'CCGG_w
Cuts at: 977 1217 1738 2416 2690 2837 3248 977
Size: 240 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 337
Size: 30 3998
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 655 688 763 238
Size: 417 33 75 3503
Fragments arranged by size:
3503 417 75 33
BseYI C'CCAG_C
Cuts at: 549 959 1453 2211 2788 3284 549
Size: 410 494 758 577 496 1293
Fragments arranged by size:
1293 758 577 496 494 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 922 619
Size: 303 3725
BsiEI CG_ry'CG
Cuts at: 104 209 233 1570 1616 2824 104
Size: 105 24 1337 46 1208 1308
Fragments arranged by size:
1337 1308 1208 105 46 24
BsiHKAI G_wGCw'C
Cuts at: 251 358 1042 1820 2010 2802 251
Size: 107 684 778 190 792 1477
Fragments arranged by size:
1477 792 778 684 190 107
BsmI GAATG_Cn'
Cuts at: 479 479
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 152 251 337 358 658 1042 1653 1746 1820
Size: 99 86 21 300 384 611 93 74
Cuts at: 1820 2010 2072 2802 152
Size: 190 62 730 1378
Fragments arranged by size:
1378 730 611 384 300 190 99 93
86 74 62 21
BspEI T'CCGG_A
Cuts at: 977 3248 977
Size: 2271 1757
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 286 1078 1292 1594 1975 286
Size: 792 214 302 381 2339
Fragments arranged by size:
2339 792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 285 678 2320 2374 3858 4023 50
Size: 235 393 1642 54 1484 165 55
Fragments arranged by size:
1642 1484 393 235 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 177 204 2026 2207 2416 177
Size: 27 1822 181 209 1789
Fragments arranged by size:
1822 1789 209 181 27
BssHII G'CGCG_C
Cuts at: 183 183
Size: 4028
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 606 1405 606
Size: 799 3229
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 468 468
Size: 4028
BstYI r'GATC_y
Cuts at: 1 374 974 1053 1520 1543 1878 2124 3136
Size: 373 600 79 467 23 335 246 1012
Cuts at: 3136 3222 3234 3580 1
Size: 86 12 346 449
Fragments arranged by size:
1012 600 467 449 373 346 335 246
86 79 23 12
Bsu36I CC'TnA_GG
Cuts at: 142 142
Size: 4028
BtgI C'CryG_G
Cuts at: 209 314 329 209
Size: 105 15 3908
Fragments arranged by size:
3908 105 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DraIII CAC_nnn'GTG
Cuts at: 315 315
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 206 230 302 1613 1787 2178 2205 3550 206
Size: 24 72 1311 174 391 27 1345 684
Fragments arranged by size:
1345 1311 684 391 174 72 27 24
EagI C'GGCC_G
Cuts at: 206 230 1613 206
Size: 24 1383 2621
Fragments arranged by size:
2621 1383 24
EarI CTCTTCn'nnn_
Cuts at: 106 970 2051 2261 106
Size: 864 1081 210 1873
Fragments arranged by size:
1873 1081 864 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 292 412 490 530 679 1853 2285 3032
Size: 51 120 78 40 149 1174 432 747
Cuts at: 3032 3300 241
Size: 268 969
Fragments arranged by size:
1174 969 747 432 268 149 120 78
51 40
EcoNI CCTnn'n_nnAGG
Cuts at: 220 220
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 157 381 423 441 1167 157
Size: 224 42 18 726 3018
Fragments arranged by size:
3018 726 224 42 18
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 166 1809 166
Size: 1643 2385
HaeII r_GCGC'y
Cuts at: 205 271 1462 1710 2732 3711 205
Size: 66 1191 248 1022 979 522
Fragments arranged by size:
1191 1022 979 522 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 879 900 911 932 1870 1900 1902 1932 879
Size: 21 11 21 938 30 2 30 2975
Fragments arranged by size:
2975 938 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 201 1458 1706 201
Size: 1257 248 2523
Fragments arranged by size:
2523 1257 248
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 304 1789 304
Size: 1485 2543
MslI CAynn'nnrTG
Cuts at: 2017 2144 2017
Size: 127 3901
MspA1I CmG'CkG
Cuts at: 331 603 971 1139 1453 1813 2826 3071 331
Size: 272 368 168 314 360 1013 245 1288
Fragments arranged by size:
1288 1013 368 360 314 272 245 168
NaeI GCC'GGC
Cuts at: 206 2209 206
Size: 2003 2025
NarI GG'CG_CC
Cuts at: 202 1459 1707 202
Size: 1257 248 2523
Fragments arranged by size:
2523 1257 248
NgoMIV G'CCGG_C
Cuts at: 204 2207 204
Size: 2003 2025
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 315 384 574 315
Size: 69 190 3769
Fragments arranged by size:
3769 190 69
PfoI T'CCnGG_A
Cuts at: 467 467
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PmlI CAC'GTG
Cuts at: 597 597
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PpuMI rG'GwC_Cy
Cuts at: 157 423 441 157
Size: 266 18 3744
Fragments arranged by size:
3744 266 18
PstI C_TGCA'G
Cuts at: 135 663 1075 1760 135
Size: 528 412 685 2403
Fragments arranged by size:
2403 685 528 412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SacII CC_GC'GG
Cuts at: 332 332
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SanDI GG'GwC_CC
Cuts at: 157 157
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 106 970 2051 2261 106
Size: 864 1081 210 1873
Fragments arranged by size:
1873 1081 864 210
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SexAI A'CCwGG_T
Cuts at: 437 437
Size: 4028
SfcI C'TryA_G
Cuts at: 33 131 659 1071 1756 2749 2940 33
Size: 98 528 412 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 412 191 98
SfoI GGC'GCC
Cuts at: 203 1460 1708 203
Size: 1257 248 2523
Fragments arranged by size:
2523 1257 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 199 1050 199
Size: 851 3177
SmlI C'TyrA_G
Cuts at: 1183 2098 2590 2852 1183
Size: 915 492 262 2359
Fragments arranged by size:
2359 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
SrfI GCCC'GGGC
Cuts at: 199 199
Size: 4028
StyI C'CwwG_G
Cuts at: 581 1162 1516 581
Size: 581 354 3093
Fragments arranged by size:
3093 581 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 520 1611 1744 2203 2412 520
Size: 1091 133 459 209 2136
Fragments arranged by size:
2136 1091 459 209 133
TatI w'GTAC_w
Cuts at: 690 3543 690
Size: 2853 1175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 3545 3577 337
Size: 32 3176 32 788
Fragments arranged by size:
3176 788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 312 1523 312
Size: 1211 2817
XmaI C'CCGG_G
Cuts at: 197 1048 197
Size: 851 3177
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmBI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI