Restriction map for
pF1KB0912
(Circular) (Six-base) MAPSORT of: pF1KB0912.seq Check: 984 from: 1 to: 4064
pF1KB0912 4064 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 527 527
Size: 4064
AatII G_ACGT'C
Cuts at: 574 574
Size: 4064
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 709 1889 2321 3068 3336 652
Size: 57 1180 432 747 268 1380
Fragments arranged by size:
1380 1180 747 432 268 57
AfeI AGC'GCT
Cuts at: 799 799
Size: 4064
AflIII A'CryG_T
Cuts at: 262 329 1416 2520 3497 262
Size: 67 1087 1104 977 829
Fragments arranged by size:
1104 1087 977 829 67
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 422 675 882 1369 2936 422
Size: 253 207 487 1567 1550
Fragments arranged by size:
1567 1550 487 253 207
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 198 1080 1494 1742 1777 198
Size: 882 414 248 35 2485
Fragments arranged by size:
2485 882 414 248 35
BanII G_rGCy'C
Cuts at: 1078 2108 1078
Size: 1030 3034
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbsI GAAGACnn'nnnn_
Cuts at: 574 574
Size: 4064
BbvCI CC'TCA_GC
Cuts at: 415 415
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglII A'GATC_T
Cuts at: 150 1579 3616 150
Size: 1429 2037 598
Fragments arranged by size:
2037 1429 598
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 1689 1782 2838 1689
Size: 93 1056 2915
Fragments arranged by size:
2915 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 234 301 1276 1357 1692 3836 234
Size: 67 975 81 335 2144 462
Fragments arranged by size:
2144 975 462 335 81 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 583 697 766 493
Size: 90 114 69 3791
Fragments arranged by size:
3791 114 90 69
Bpu10I CC'TnA_GC
Cuts at: 415 415
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 933 1417 933
Size: 484 3580
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 571 1495 1743 2445 3941 571
Size: 924 248 702 1496 694
Fragments arranged by size:
1496 924 702 694 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 556 586 556
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 694 199
Size: 495 3569
BseYI C'CCAG_C
Cuts at: 746 1489 2247 2824 3320 746
Size: 743 758 577 496 1490
Fragments arranged by size:
1490 758 743 577 496
BsiEI CG_ry'CG
Cuts at: 104 293 1606 1652 2860 104
Size: 189 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 189 46
BsiHKAI G_wGCw'C
Cuts at: 431 782 1078 1856 2046 2838 431
Size: 351 296 778 190 792 1657
Fragments arranged by size:
1657 792 778 351 296 190
Bsp1286I G_dGCh'C
Cuts at: 431 782 1078 1689 1782 1856 2046 2108 2838
Size: 351 296 611 93 74 190 62 730
Cuts at: 2838 431
Size: 1657
Fragments arranged by size:
1657 730 611 351 296 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 1028 3240 1028
Size: 2212 1852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 527 1114 1328 1630 2011 527
Size: 587 214 302 381 2580
Fragments arranged by size:
2580 587 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 859 2335 2693 859
Size: 1476 358 2230
Fragments arranged by size:
2230 1476 358
BstAPI GCAn_nnn'nTGC
Cuts at: 230 1441 230
Size: 1211 2853
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstYI r'GATC_y
Cuts at: 1 150 701 1089 1556 1579 1914 2160 3172
Size: 149 551 388 467 23 335 246 1012
Cuts at: 3172 3258 3270 3616 1
Size: 86 12 346 449
Fragments arranged by size:
1012 551 467 449 388 346 335 246
149 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 537 2014 2195 2358 537
Size: 1477 181 163 2243
Fragments arranged by size:
2243 1477 181 163
BtsI GCAGTG_nn'
Cuts at: 177 657 1307 177
Size: 480 650 2934
Fragments arranged by size:
2934 650 480
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DraIII CAC_nnn'GTG
Cuts at: 242 242
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 884 935 1649 1823 2214 2241 3586 884
Size: 51 714 174 391 27 1345 1362
Fragments arranged by size:
1362 1345 714 391 174 51 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 1046 2087 2297 1046
Size: 1041 210 2813
Fragments arranged by size:
2813 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 613 2272 2582 2728 3874 613
Size: 1659 310 146 1146 803
Fragments arranged by size:
1659 1146 803 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 583 652 697 709 766 1889 2321 3068
Size: 90 69 45 12 57 1123 432 747
Cuts at: 3068 3336 493
Size: 268 1221
Fragments arranged by size:
1221 1123 747 432 268 90 69 57
45 12
EcoO109I rG'GnC_Cy
Cuts at: 1203 1203
Size: 4064
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1040 1845 1040
Size: 805 3259
HaeII r_GCGC'y
Cuts at: 801 1498 1746 2768 3747 801
Size: 697 248 1022 979 1118
Fragments arranged by size:
1118 1022 979 697 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 743 750 775 782 804 836 1906
Size: 32 155 7 25 7 22 32 1070
Cuts at: 1906 1936 1938 1968 556
Size: 30 2 30 2652
Fragments arranged by size:
2652 1070 155 32 32 30 30 25
22 7 7 2
HincII GTy'rAC
Cuts at: 144 657 837 1103 144
Size: 513 180 266 3105
Fragments arranged by size:
3105 513 266 180
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MfeI C'AATT_G
Cuts at: 686 686
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 886 937 1825 886
Size: 51 888 3125
Fragments arranged by size:
3125 888 51
MslI CAynn'nnrTG
Cuts at: 2053 2180 2053
Size: 127 3937
MspA1I CmG'CkG
Cuts at: 577 594 621 746 1175 1489 1849 2862 3107
Size: 17 27 125 429 314 360 1013 245
Cuts at: 3107 577
Size: 1534
Fragments arranged by size:
1534 1013 429 360 314 245 125 27
17
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 107 107
Size: 4064
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 217 266 333 639 1117 1617 2524 217
Size: 49 67 306 478 500 907 1757
Fragments arranged by size:
1757 907 500 478 306 67 49
PasI CC'CwG_GG
Cuts at: 876 876
Size: 4064
PciI A'CATG_T
Cuts at: 262 329 2520 262
Size: 67 2191 1806
Fragments arranged by size:
2191 1806 67
PflMI CCAn_nnn'nTGG
Cuts at: 882 993 882
Size: 111 3953
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 901 933 901
Size: 32 4032
PstI C_TGCA'G
Cuts at: 347 1111 1796 347
Size: 764 685 2615
Fragments arranged by size:
2615 764 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 577 594 621 1489 1849 577
Size: 17 27 868 360 2792
Fragments arranged by size:
2792 868 360 27 17
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 343 375 1107 1792 2785 2976 33
Size: 310 32 732 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 310 191 32
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 1219 2134 2626 2888 1219
Size: 915 492 262 2395
Fragments arranged by size:
2395 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StuI AGG'CCT
Cuts at: 1022 1022
Size: 4064
StyI C'CwwG_G
Cuts at: 107 1198 1552 107
Size: 1091 354 2619
Fragments arranged by size:
2619 1091 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 702 1647 1780 2239 2448 253
Size: 449 945 133 459 209 1869
Fragments arranged by size:
1869 945 459 449 209 133
TatI w'GTAC_w
Cuts at: 192 3579 192
Size: 3387 677
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
ZraI GAC'GTC
Cuts at: 572 572
Size: 4064
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI