Restriction map for
pF1KB0915
(Circular) (Six-base) MAPSORT of: pF1KB0915.seq Check: 7063 from: 1 to: 3452
pF1KB0915 3452 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 490 490
Size: 3452
Acc65I G'GTAC_C
Cuts at: 468 468
Size: 3452
AclI AA'CG_TT
Cuts at: 3292 3292
Size: 3452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1709 2456 2724 1277
Size: 432 747 268 2005
Fragments arranged by size:
2005 747 432 268
AflIII A'CryG_T
Cuts at: 804 1908 2885 804
Size: 1104 977 1371
Fragments arranged by size:
1371 1104 977
AgeI A'CCGG_T
Cuts at: 208 1840 208
Size: 1632 1820
AhdI GACnn_n'nnGTC
Cuts at: 209 209
Size: 3452
AlwNI CAG_nnn'CTG
Cuts at: 372 757 2324 372
Size: 385 1567 1500
Fragments arranged by size:
1567 1500 385
ApaLI G'TGCA_C
Cuts at: 285 2222 285
Size: 1937 1515
ApoI r'AATT_y
Cuts at: 456 2805 3061 456
Size: 2349 256 847
Fragments arranged by size:
2349 847 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
AvaI C'yCGr_G
Cuts at: 472 472
Size: 3452
BamHI G'GATC_C
Cuts at: 477 477
Size: 3452
BanI G'GyrC_C
Cuts at: 468 882 1130 1165 468
Size: 414 248 35 2755
Fragments arranged by size:
2755 414 248 35
BanII G_rGCy'C
Cuts at: 466 1496 466
Size: 1030 2422
BbeI G_GCGC'C
Cuts at: 886 1134 886
Size: 248 3204
BciVI GTATCCnnnnn_n'
Cuts at: 1350 2111 3452 1350
Size: 761 1341 1350
Fragments arranged by size:
1350 1341 761
BclI T'GATC_A
Cuts at: 972 972
Size: 3452
BglII A'GATC_T
Cuts at: 967 3004 967
Size: 2037 1415
BlpI GC'TnA_GC
Cuts at: 564 564
Size: 3452
Bme1580I G_kGCm'C
Cuts at: 289 296 328 1077 1170 2226 289
Size: 7 32 749 93 1056 1515
Fragments arranged by size:
1515 1056 749 93 32 7
BmrI ACTGGGnnnn_n'
Cuts at: 664 745 1080 3224 664
Size: 81 335 2144 892
Fragments arranged by size:
2144 892 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1507 1999 2297 628
Size: 879 492 298 1783
Fragments arranged by size:
1783 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3156 217
Size: 2939 513
BsaAI yAC'GTr
Cuts at: 151 805 151
Size: 654 2798
BsaBI GATnn'nnATC
Cuts at: 6 966 3003 6
Size: 960 2037 455
Fragments arranged by size:
2037 960 455
BsaHI Gr'CG_yC
Cuts at: 883 1131 1833 3329 883
Size: 248 702 1496 1006
Fragments arranged by size:
1496 1006 702 248
BsaWI w'CCGG_w
Cuts at: 208 641 1162 1840 2114 2261 2672 208
Size: 433 521 678 274 147 411 988
Fragments arranged by size:
988 678 521 433 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 135 135
Size: 3452
BseYI C'CCAG_C
Cuts at: 877 1635 2212 2708 877
Size: 758 577 496 1621
Fragments arranged by size:
1621 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 994 1040 2248 104
Size: 890 46 1208 1308
Fragments arranged by size:
1308 1208 890 46
BsiHKAI G_wGCw'C
Cuts at: 289 466 1244 1434 2226 289
Size: 177 778 190 792 1515
Fragments arranged by size:
1515 792 778 190 177
Bsp1286I G_dGCh'C
Cuts at: 289 296 328 466 1077 1170 1244 1434 1496
Size: 7 32 138 611 93 74 190 62
Cuts at: 1496 2226 289
Size: 730 1515
Fragments arranged by size:
1515 730 611 190 138 93 74 62
32 7
BspEI T'CCGG_A
Cuts at: 2672 2672
Size: 3452
BspHI T'CATG_A
Cuts at: 215 2628 215
Size: 2413 1039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 502 716 1018 1399 502
Size: 214 302 381 2555
Fragments arranged by size:
2555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1744 1798 3282 3447 50
Size: 1694 54 1484 165 55
Fragments arranged by size:
1694 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1364 1364
Size: 3452
BsrFI r'CCGG_y
Cuts at: 208 1450 1631 1840 208
Size: 1242 181 209 1820
Fragments arranged by size:
1820 1242 209 181
BssSI C'ACGA_G
Cuts at: 1723 2081 1723
Size: 358 3094
BstAPI GCAn_nnn'nTGC
Cuts at: 829 829
Size: 3452
BstBI TT'CG_AA
Cuts at: 1813 1813
Size: 3452
BstYI r'GATC_y
Cuts at: 1 477 944 967 1302 1548 2560 2646 2658
Size: 476 467 23 335 246 1012 86 12
Cuts at: 2658 3004 1
Size: 346 449
Fragments arranged by size:
1012 476 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1402 1583 1746 1402
Size: 181 163 3108
Fragments arranged by size:
3108 181 163
BtsI GCAGTG_nn'
Cuts at: 695 695
Size: 3452
DraI TTT'AAA
Cuts at: 451 451
Size: 3452
DrdI GACnn_nn'nnGTC
Cuts at: 1158 2016 1158
Size: 858 2594
EaeI y'GGCC_r
Cuts at: 301 1037 1211 1602 1629 2974 301
Size: 736 174 391 27 1345 779
Fragments arranged by size:
1345 779 736 391 174 27
EagI C'GGCC_G
Cuts at: 1037 1037
Size: 3452
EarI CTCTTCn'nnn_
Cuts at: 384 1475 1685 384
Size: 1091 210 2151
Fragments arranged by size:
2151 1091 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 1970 2116 3262 1660
Size: 310 146 1146 1850
Fragments arranged by size:
1850 1146 310 146
EcoICRI GAG'CTC
Cuts at: 464 464
Size: 3452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1709 2456 2724 1277
Size: 432 747 268 2005
Fragments arranged by size:
2005 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 591 591
Size: 3452
EcoRI G'AATT_C
Cuts at: 456 456
Size: 3452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1471 1471
Size: 3452
FspI TGC'GCA
Cuts at: 1233 1233
Size: 3452
HaeII r_GCGC'y
Cuts at: 886 1134 2156 3135 886
Size: 248 1022 979 1203
Fragments arranged by size:
1203 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 1294 1324 1326 1356 195
Size: 32 1067 30 2 30 2291
Fragments arranged by size:
2291 1067 32 30 30 2
HincII GTy'rAC
Cuts at: 491 491
Size: 3452
KasI G'GCGC_C
Cuts at: 882 1130 882
Size: 248 3204
KpnI G_GTAC'C
Cuts at: 472 472
Size: 3452
MfeI C'AATT_G
Cuts at: 121 121
Size: 3452
MluI A'CGCG_T
Cuts at: 2885 2885
Size: 3452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2307 2123
Size: 184 3268
MscI TGG'CCA
Cuts at: 1213 1213
Size: 3452
MslI CAynn'nnrTG
Cuts at: 1441 1568 1441
Size: 127 3325
MspA1I CmG'CkG
Cuts at: 563 877 1237 2250 2495 563
Size: 314 360 1013 245 1520
Fragments arranged by size:
1520 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1633 1633
Size: 3452
NarI GG'CG_CC
Cuts at: 883 1131 883
Size: 248 3204
NcoI C'CATG_G
Cuts at: 107 107
Size: 3452
NgoMIV G'CCGG_C
Cuts at: 1631 1631
Size: 3452
NspI r_CATG'y
Cuts at: 505 1005 1912 505
Size: 500 907 2045
Fragments arranged by size:
2045 907 500
PciI A'CATG_T
Cuts at: 1908 1908
Size: 3452
PfoI T'CCnGG_A
Cuts at: 128 128
Size: 3452
PmeI GTTT'AAAC
Cuts at: 451 451
Size: 3452
PmlI CAC'GTG
Cuts at: 151 151
Size: 3452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 3420
PstI C_TGCA'G
Cuts at: 499 1184 499
Size: 685 2767
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3452
PvuII CAG'CTG
Cuts at: 877 1237 877
Size: 360 3092
RsrII CG'GwC_CG
Cuts at: 1647 1647
Size: 3452
SacI G_AGCT'C
Cuts at: 466 466
Size: 3452
SalI G'TCGA_C
Cuts at: 489 489
Size: 3452
SapI GCTCTTCn'nnn_
Cuts at: 1475 1685 1475
Size: 210 3242
SbfI CC_TGCA'GG
Cuts at: 499 499
Size: 3452
ScaI AGT'ACT
Cuts at: 2969 2969
Size: 3452
SfcI C'TryA_G
Cuts at: 33 495 1180 2173 2364 33
Size: 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 191
SfoI GGC'GCC
Cuts at: 884 1132 884
Size: 248 3204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
SmaI CCC'GGG
Cuts at: 474 474
Size: 3452
SmlI C'TyrA_G
Cuts at: 607 1522 2014 2276 607
Size: 915 492 262 1783
Fragments arranged by size:
1783 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3452
SphI G_CATG'C
Cuts at: 505 1005 505
Size: 500 2952
StuI AGG'CCT
Cuts at: 161 161
Size: 3452
StyI C'CwwG_G
Cuts at: 107 304 586 940 107
Size: 197 282 354 2619
Fragments arranged by size:
2619 354 282 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 149 186 1035 1168 1627 1836 149
Size: 37 849 133 459 209 1765
Fragments arranged by size:
1765 849 459 209 133 37
TatI w'GTAC_w
Cuts at: 2967 2967
Size: 3452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3420
Tth111I GACn'n_nGTC
Cuts at: 1249 1249
Size: 3452
XbaI T'CTAG_A
Cuts at: 483 483
Size: 3452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 947 947
Size: 3452
XmaI C'CCGG_G
Cuts at: 472 472
Size: 3452
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI