Restriction map for
pF1KB0916
(Circular) (Six-base) MAPSORT of: pF1KB0916.seq Check: 1313 from: 1 to: 4097
pF1KB0916 4097 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 906 1135 906
Size: 229 3868
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1922 2354 3101 3369 691
Size: 1231 432 747 268 1419
Fragments arranged by size:
1419 1231 747 432 268
AflIII A'CryG_T
Cuts at: 284 635 1024 1449 2553 3530 284
Size: 351 389 425 1104 977 851
Fragments arranged by size:
1104 977 851 425 389 351
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AleI CACnn'nnGTG
Cuts at: 975 975
Size: 4097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 993 993
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 280 1402 2969 280
Size: 1122 1567 1408
Fragments arranged by size:
1567 1408 1122
ApaI G_GGCC'C
Cuts at: 416 416
Size: 4097
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 319 787 1101 3450 3706 319
Size: 468 314 2349 256 710
Fragments arranged by size:
2349 710 468 314 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 408 465 1117 408
Size: 57 652 3388
Fragments arranged by size:
3388 652 57
AvrII C'CTAG_G
Cuts at: 525 525
Size: 4097
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 400 800 842 1113 1527 1775 1810 400
Size: 400 42 271 414 248 35 2687
Fragments arranged by size:
2687 414 400 271 248 42 35
BanII G_rGCy'C
Cuts at: 416 567 1001 1111 2141 416
Size: 151 434 110 1030 2372
Fragments arranged by size:
2372 1030 434 151 110
BbeI G_GCGC'C
Cuts at: 404 1531 1779 404
Size: 1127 248 2722
Fragments arranged by size:
2722 1127 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 168 202 168
Size: 34 4063
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 583 622 1209 583
Size: 39 587 3471
Fragments arranged by size:
3471 587 39
Bme1580I G_kGCm'C
Cuts at: 416 805 1722 1815 2871 416
Size: 389 917 93 1056 1642
Fragments arranged by size:
1642 1056 917 389 93
BmgBI CAC'GTC
Cuts at: 285 1027 285
Size: 742 3355
BmrI ACTGGGnnnn_n'
Cuts at: 997 1003 1309 1390 1725 3869 997
Size: 6 306 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 306 81 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 480 2464 480
Size: 1984 2113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 832 802
Size: 30 4067
Bpu10I CC'TnA_GC
Cuts at: 806 806
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 387 401 1528 1776 2478 3974 387
Size: 14 1127 248 702 1496 510
Fragments arranged by size:
1496 1127 702 510 248 14
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 959 983 989 1013 959
Size: 24 6 24 4043
Fragments arranged by size:
4043 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1044 1072 1044
Size: 28 4069
BseYI C'CCAG_C
Cuts at: 614 818 838 1062 1522 2280 2857 3353 614
Size: 204 20 224 460 758 577 496 1358
Fragments arranged by size:
1358 758 577 496 460 224 204 20
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 217 567 972 1111 1889 2079 2871 217
Size: 350 405 139 778 190 792 1443
Fragments arranged by size:
1443 792 778 405 350 190 139
BsmI GAATG_Cn'
Cuts at: 434 434
Size: 4097
BsmBI CGTCTCn'nnnn_
Cuts at: 738 1033 738
Size: 295 3802
Bsp1286I G_dGCh'C
Cuts at: 217 416 567 805 972 1001 1111 1722 1815
Size: 199 151 238 167 29 110 611 93
Cuts at: 1815 1889 2079 2141 2871 217
Size: 74 190 62 730 1443
Fragments arranged by size:
1443 730 611 238 199 190 167 151
110 93 74 62 29
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1361 1663 2044 1147
Size: 214 302 381 3200
Fragments arranged by size:
3200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 419 833 2009 419
Size: 414 1176 2507
Fragments arranged by size:
2507 1176 414
BsrFI r'CCGG_y
Cuts at: 249 2095 2276 2485 249
Size: 1846 181 209 1861
Fragments arranged by size:
1861 1846 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 838 1074 838
Size: 236 3861
BstYI r'GATC_y
Cuts at: 1 266 1122 1589 1612 1947 2193 3205 3291
Size: 265 856 467 23 335 246 1012 86
Cuts at: 3291 3303 3649 1
Size: 12 346 449
Fragments arranged by size:
1012 856 467 449 346 335 265 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 928 107
Size: 821 3276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 159 652 1340 159
Size: 493 688 2916
Fragments arranged by size:
2916 688 493
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 110 247 494 1682 1856 2247 2274 3619 110
Size: 137 247 1188 174 391 27 1345 588
Fragments arranged by size:
1345 1188 588 391 247 174 137 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 508 2120 2330 508
Size: 1612 210 2275
Fragments arranged by size:
2275 1612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 565 1109 565
Size: 544 3553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 802 832 1922 2354 3101 3369 691
Size: 111 30 1090 432 747 268 1419
Fragments arranged by size:
1419 1090 747 432 268 111 30
EcoNI CCTnn'n_nnAGG
Cuts at: 962 962
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 122 277 304 347 412 413 1236 122
Size: 155 27 43 65 1 823 2983
Fragments arranged by size:
2983 823 155 65 43 27 1
EcoRI G'AATT_C
Cuts at: 319 1101 319
Size: 782 3315
EcoRV GAT'ATC
Cuts at: 1011 1011
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 404 1531 1779 2801 3780 404
Size: 1127 248 1022 979 721
Fragments arranged by size:
1127 1022 979 721 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 888 920 983 1015 1939 1969 1971 2001 888
Size: 32 63 32 924 30 2 30 2984
Fragments arranged by size:
2984 924 63 32 32 30 30 2
HincII GTy'rAC
Cuts at: 195 907 1136 195
Size: 712 229 3156
Fragments arranged by size:
3156 712 229
KasI G'GCGC_C
Cuts at: 400 1527 1775 400
Size: 1127 248 2722
Fragments arranged by size:
2722 1127 248
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MfeI C'AATT_G
Cuts at: 490 490
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 496 1858 496
Size: 1362 2735
MslI CAynn'nnrTG
Cuts at: 975 2086 2213 975
Size: 1111 127 2859
Fragments arranged by size:
2859 1111 127
MspA1I CmG'CkG
Cuts at: 798 852 1208 1522 1882 2895 3140 798
Size: 54 356 314 360 1013 245 1755
Fragments arranged by size:
1755 1013 360 356 314 245 54
NaeI GCC'GGC
Cuts at: 251 2278 251
Size: 2027 2070
NarI GG'CG_CC
Cuts at: 401 1528 1776 401
Size: 1127 248 2722
Fragments arranged by size:
2722 1127 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 249 2276 249
Size: 2027 2070
NspI r_CATG'y
Cuts at: 639 1150 1650 2557 639
Size: 511 500 907 2179
Fragments arranged by size:
2179 907 511 500
PasI CC'CwG_GG
Cuts at: 300 300
Size: 4097
PciI A'CATG_T
Cuts at: 635 2553 635
Size: 1918 2179
PflMI CCAn_nnn'nTGG
Cuts at: 154 638 154
Size: 484 3613
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 304 304
Size: 4097
PspOMI G'GGCC_C
Cuts at: 412 412
Size: 4097
PstI C_TGCA'G
Cuts at: 346 857 1041 1144 1829 346
Size: 511 184 103 685 2614
Fragments arranged by size:
2614 685 511 184 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 852 1522 1882 852
Size: 670 360 3067
Fragments arranged by size:
3067 670 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 567 1111 567
Size: 544 3553
SalI G'TCGA_C
Cuts at: 905 1134 905
Size: 229 3868
SanDI GG'GwC_CC
Cuts at: 304 304
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 346 1144 346
Size: 798 3299
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SexAI A'CCwGG_T
Cuts at: 205 205
Size: 4097
SfcI C'TryA_G
Cuts at: 33 342 853 1037 1140 1825 2818 3009 33
Size: 309 511 184 103 685 993 191 1121
Fragments arranged by size:
1121 993 685 511 309 191 184 103
SfoI GGC'GCC
Cuts at: 402 1529 1777 402
Size: 1127 248 2722
Fragments arranged by size:
2722 1127 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 410 1119 410
Size: 709 3388
SmlI C'TyrA_G
Cuts at: 1252 2167 2659 2921 1252
Size: 915 492 262 2428
Fragments arranged by size:
2428 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 107 497 525 1231 1585 107
Size: 390 28 706 354 2619
Fragments arranged by size:
2619 706 390 354 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 830 1680 1813 2272 2481 232
Size: 598 850 133 459 209 1848
Fragments arranged by size:
1848 850 598 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 959 991 3614 3646 305
Size: 32 622 32 2623 32 756
Fragments arranged by size:
2623 756 622 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 226 1592 226
Size: 1366 2731
XmaI C'CCGG_G
Cuts at: 408 1117 408
Size: 709 3388
XmnI GAAnn'nnTTC
Cuts at: 790 790
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BbsI BbvCI BfrBI BglI BmtI BsgI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI