Restriction map for
pF1KB0922
(Circular) (Six-base) MAPSORT of: pF1KB0922.seq Check: 9644 from: 1 to: 4148
pF1KB0922 4148 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1048 1164 1048
Size: 116 4032
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1119 1973 2405 3152 3420 1119
Size: 854 432 747 268 1847
Fragments arranged by size:
1847 854 747 432 268
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 655 1078 655
Size: 423 3725
AlwNI CAG_nnn'CTG
Cuts at: 660 916 1453 3020 660
Size: 256 537 1567 1788
Fragments arranged by size:
1788 1567 537 256
ApaI G_GGCC'C
Cuts at: 348 362 1010 348
Size: 14 648 3486
Fragments arranged by size:
3486 648 14
ApaLI G'TGCA_C
Cuts at: 253 2918 253
Size: 2665 1483
ApoI r'AATT_y
Cuts at: 169 1152 3501 3757 169
Size: 983 2349 256 560
Fragments arranged by size:
2349 983 560 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 264 751 1168 264
Size: 487 417 3244
Fragments arranged by size:
3244 487 417
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 471 1048 1164 1578 1826 1861 471
Size: 577 116 414 248 35 2758
Fragments arranged by size:
2758 577 414 248 116 35
BanII G_rGCy'C
Cuts at: 348 362 455 1010 1162 2192 348
Size: 14 93 555 152 1030 2304
Fragments arranged by size:
2304 1030 555 152 93 14
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BbsI GAAGACnn'nnnn_
Cuts at: 787 787
Size: 4148
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglI GCCn_nnn'nGGC
Cuts at: 611 611
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 926 1260 926
Size: 334 3814
Bme1580I G_kGCm'C
Cuts at: 257 348 362 1010 1773 1866 2922 257
Size: 91 14 648 763 93 1056 1483
Fragments arranged by size:
1483 1056 763 648 93 91 14
BmgBI CAC'GTC
Cuts at: 276 546 276
Size: 270 3878
BmrI ACTGGGnnnn_n'
Cuts at: 977 1360 1441 1776 3920 977
Size: 383 81 335 2144 1205
Fragments arranged by size:
2144 1205 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 682 387
Size: 295 3853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 556 3852 556
Size: 3296 852
BsaAI yAC'GTr
Cuts at: 700 1501 700
Size: 801 3347
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 176 1579 1827 2529 4025 176
Size: 1403 248 702 1496 299
Fragments arranged by size:
1496 1403 702 299 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 885 915 885
Size: 30 4118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1133 1133
Size: 4148
BseYI C'CCAG_C
Cuts at: 1016 1100 1573 2331 2908 3404 1016
Size: 84 473 758 577 496 1760
Fragments arranged by size:
1760 758 577 496 473 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 403 568 1009 322
Size: 81 165 441 3461
Fragments arranged by size:
3461 441 165 81
BsiEI CG_ry'CG
Cuts at: 104 118 1690 1736 2944 104
Size: 14 1572 46 1208 1308
Fragments arranged by size:
1572 1308 1208 46 14
BsiHKAI G_wGCw'C
Cuts at: 257 1162 1940 2130 2922 257
Size: 905 778 190 792 1483
Fragments arranged by size:
1483 905 792 778 190
BsmI GAATG_Cn'
Cuts at: 1112 1112
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 257 348 362 455 1010 1162 1773 1866 1940
Size: 91 14 93 555 152 611 93 74
Cuts at: 1940 2130 2192 2922 257
Size: 190 62 730 1483
Fragments arranged by size:
1483 730 611 555 190 152 93 93
91 74 62 14
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 317 1031 1198 1412 1714 2095 317
Size: 714 167 214 302 381 2370
Fragments arranged by size:
2370 714 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 218 666 2440 2494 3978 4143 50
Size: 168 448 1774 54 1484 165 55
Fragments arranged by size:
1774 1484 448 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BssHII G'CGCG_C
Cuts at: 133 133
Size: 4148
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstXI CCAn_nnnn'nTGG
Cuts at: 411 411
Size: 4148
BstYI r'GATC_y
Cuts at: 1 260 746 1173 1640 1663 1998 2244 3256
Size: 259 486 427 467 23 335 246 1012
Cuts at: 3256 3342 3354 3700 1
Size: 86 12 346 449
Fragments arranged by size:
1012 486 467 449 427 346 335 259
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 367 957 1391 367
Size: 590 434 3124
Fragments arranged by size:
3124 590 434
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 1854 2712 1854
Size: 858 3290
EaeI y'GGCC_r
Cuts at: 115 350 463 487 1733 1907 2298 2325 3670
Size: 235 113 24 1246 174 391 27 1345
Cuts at: 3670 115
Size: 593
Fragments arranged by size:
1345 1246 593 391 235 174 113 27
24
EagI C'GGCC_G
Cuts at: 115 1733 115
Size: 1618 2530
EarI CTCTTCn'nnn_
Cuts at: 157 2171 2381 157
Size: 2014 210 1924
Fragments arranged by size:
2014 1924 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 511 2356 2666 2812 3958 511
Size: 1845 310 146 1146 701
Fragments arranged by size:
1845 1146 701 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 682 1119 1973 2405 3152 3420 387
Size: 295 437 854 432 747 268 1115
Fragments arranged by size:
1115 854 747 437 432 295 268
EcoO109I rG'GnC_Cy
Cuts at: 345 359 657 898 913 1006 1007 1020 1287
Size: 14 298 241 15 93 1 13 267
Cuts at: 1287 345
Size: 3206
Fragments arranged by size:
3206 298 267 241 93 15 14 13
1
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 481 1929 481
Size: 1448 2700
HaeII r_GCGC'y
Cuts at: 1582 1830 2852 3831 1582
Size: 248 1022 979 1899
Fragments arranged by size:
1899 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 885 917 1990 2020 2022 2052 885
Size: 32 1073 30 2 30 2981
Fragments arranged by size:
2981 1073 32 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1052 1168 1052
Size: 116 4032
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 210 449 2819 3003 210
Size: 239 2370 184 1355
Fragments arranged by size:
2370 1355 239 184
MscI TGG'CCA
Cuts at: 352 1909 352
Size: 1557 2591
MslI CAynn'nnrTG
Cuts at: 544 590 637 2137 2264 544
Size: 46 47 1500 127 2428
Fragments arranged by size:
2428 1500 127 47 46
MspA1I CmG'CkG
Cuts at: 356 407 438 1259 1573 1933 2946 3191 356
Size: 51 31 821 314 360 1013 245 1313
Fragments arranged by size:
1313 1013 821 360 314 245 51 31
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NcoI C'CATG_G
Cuts at: 107 107
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 1201 1701 2608 1201
Size: 500 907 2741
Fragments arranged by size:
2741 907 500
PasI CC'CwG_GG
Cuts at: 609 654 609
Size: 45 4103
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PflMI CCAn_nnn'nTGG
Cuts at: 660 660
Size: 4148
PfoI T'CCnGG_A
Cuts at: 224 672 224
Size: 448 3700
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 898 1020 898
Size: 122 4026
PspOMI G'GGCC_C
Cuts at: 344 358 1006 344
Size: 14 648 3486
Fragments arranged by size:
3486 648 14
PspXI vC'TCGA_Gb
Cuts at: 751 751
Size: 4148
PstI C_TGCA'G
Cuts at: 437 912 1195 1880 437
Size: 475 283 685 2705
Fragments arranged by size:
2705 685 475 283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1573 1933 1573
Size: 360 3788
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 157 2171 2381 157
Size: 2014 210 1924
Fragments arranged by size:
2014 1924 210
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SexAI A'CCwGG_T
Cuts at: 956 956
Size: 4148
SfcI C'TryA_G
Cuts at: 33 433 908 1191 1876 2869 3060 33
Size: 400 475 283 685 993 191 1121
Fragments arranged by size:
1121 993 685 475 400 283 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 266 1170 266
Size: 904 3244
SmlI C'TyrA_G
Cuts at: 751 1303 2218 2710 2972 751
Size: 552 915 492 262 1927
Fragments arranged by size:
1927 915 552 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StyI C'CwwG_G
Cuts at: 107 785 1282 1636 107
Size: 678 497 354 2619
Fragments arranged by size:
2619 678 497 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1731 1864 2323 2532 1731
Size: 133 459 209 3347
Fragments arranged by size:
3347 459 209 133
TatI w'GTAC_w
Cuts at: 718 3663 718
Size: 2945 1203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 958 1945 958
Size: 987 3161
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XhoI C'TCGA_G
Cuts at: 751 751
Size: 4148
XmaI C'CCGG_G
Cuts at: 264 1168 264
Size: 904 3244
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI