Restriction map for
pF1KB0926
(Circular) (Six-base) MAPSORT of: pF1KB0926.seq Check: 8722 from: 1 to: 4181
pF1KB0926 4181 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 458 458
Size: 4181
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1026 1102 1135 1180 2006 2438 3185 3453
Size: 504 76 33 45 826 432 747 268
Cuts at: 3453 522
Size: 1250
Fragments arranged by size:
1250 826 747 504 432 268 76 45
33
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 263 1087 263
Size: 824 3357
AlwNI CAG_nnn'CTG
Cuts at: 372 450 563 1020 1486 3053 372
Size: 78 113 457 466 1567 1500
Fragments arranged by size:
1567 1500 466 457 113 78
ApaI G_GGCC'C
Cuts at: 257 305 257
Size: 48 4133
ApaLI G'TGCA_C
Cuts at: 2951 2951
Size: 4181
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 304 1201 304
Size: 897 3284
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 837 1197 1611 1859 1894 837
Size: 360 414 248 35 3124
Fragments arranged by size:
3124 414 360 248 35
BanII G_rGCy'C
Cuts at: 152 257 305 712 1040 1195 2225 152
Size: 105 48 407 328 155 1030 2108
Fragments arranged by size:
2108 1030 407 328 155 105 48
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BbsI GAAGACnn'nnnn_
Cuts at: 369 812 369
Size: 443 3738
BbvCI CC'TCA_GC
Cuts at: 157 847 157
Size: 690 3491
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 1701 1701
Size: 4181
BglI GCCn_nnn'nGGC
Cuts at: 208 309 208
Size: 101 4080
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 1293 1293
Size: 4181
Bme1580I G_kGCm'C
Cuts at: 257 305 1806 1899 2955 257
Size: 48 1501 93 1056 1483
Fragments arranged by size:
1501 1483 1056 93 48
BmgBI CAC'GTC
Cuts at: 984 984
Size: 4181
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 776 839 846 283
Size: 493 63 7 3618
Fragments arranged by size:
3618 493 63 7
Bpu10I CC'TnA_GC
Cuts at: 117 157 847 117
Size: 40 690 3451
Fragments arranged by size:
3451 690 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 1357 2236 2728 3026 894
Size: 463 879 492 298 2049
Fragments arranged by size:
2049 879 492 463 298
BsaI GGTCTCn'nnnn_
Cuts at: 3885 3885
Size: 4181
BsaAI yAC'GTr
Cuts at: 1032 1534 1032
Size: 502 3679
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 1004 1612 1860 2562 4058 1004
Size: 608 248 702 1496 1127
Fragments arranged by size:
1496 1127 702 608 248
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2843 2990 3401 1370
Size: 521 678 274 147 411 2150
Fragments arranged by size:
2150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 694 709 724 739 145
Size: 30 519 15 15 15 3587
Fragments arranged by size:
3587 519 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 714 717 714
Size: 3 4178
BseYI C'CCAG_C
Cuts at: 190 1119 1606 2364 2941 3437 190
Size: 929 487 758 577 496 934
Fragments arranged by size:
934 929 758 577 496 487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 4181
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1040 1195 1973 2163 2955 1040
Size: 155 778 190 792 2266
Fragments arranged by size:
2266 792 778 190 155
Bsp1286I G_dGCh'C
Cuts at: 152 257 305 712 1040 1195 1806 1899 1973
Size: 105 48 407 328 155 611 93 74
Cuts at: 1973 2163 2225 2955 152
Size: 190 62 730 1378
Fragments arranged by size:
1378 730 611 407 328 190 155 105
93 74 62 48
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 1097 3357 1097
Size: 2260 1921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 458 1231 1445 1747 2128 458
Size: 773 214 302 381 2511
Fragments arranged by size:
2511 773 381 302 214
BsrBI CCG'CTC
Cuts at: 50 309 2473 2527 4011 4176 50
Size: 259 2164 54 1484 165 55
Fragments arranged by size:
2164 1484 259 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 2179 2360 2569 2179
Size: 181 209 3791
Fragments arranged by size:
3791 209 181
BssSI C'ACGA_G
Cuts at: 1051 2452 2810 1051
Size: 1401 358 2422
Fragments arranged by size:
2422 1401 358
BstAPI GCAn_nnn'nTGC
Cuts at: 353 1558 353
Size: 1205 2976
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstYI r'GATC_y
Cuts at: 1 1206 1673 1696 2031 2277 3289 3375 3387
Size: 1205 467 23 335 246 1012 86 12
Cuts at: 3387 3733 1
Size: 346 449
Fragments arranged by size:
1205 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 170 170
Size: 4181
BtgI C'CryG_G
Cuts at: 133 182 946 133
Size: 49 764 3368
Fragments arranged by size:
3368 764 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 542 2131 2312 2475 542
Size: 1589 181 163 2248
Fragments arranged by size:
2248 1589 181 163
BtsI GCAGTG_nn'
Cuts at: 573 1424 573
Size: 851 3330
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 310 943 1766 1940 2331 2358 3703 310
Size: 633 823 174 391 27 1345 788
Fragments arranged by size:
1345 823 788 633 391 174 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 541 629 1045 1078 2204 2414 541
Size: 88 416 33 1126 210 2308
Fragments arranged by size:
2308 1126 416 210 88 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 675 954 2389 2699 2845 3991 675
Size: 279 1435 310 146 1146 865
Fragments arranged by size:
1435 1146 865 310 279 146
EcoICRI GAG'CTC
Cuts at: 1038 1193 1038
Size: 155 4026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 522 776 839 846 1026 1102 1135 1180
Size: 239 254 63 7 180 76 33 45
Cuts at: 1180 2006 2438 3185 3453 283
Size: 826 432 747 268 1011
Fragments arranged by size:
1011 826 747 432 268 254 239 180
76 63 45 33 7
EcoNI CCTnn'n_nnAGG
Cuts at: 555 555
Size: 4181
EcoO109I rG'GnC_Cy
Cuts at: 253 254 1024 1123 1320 253
Size: 1 770 99 197 3114
Fragments arranged by size:
3114 770 197 99 1
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 393 2200 393
Size: 1807 2374
FspI TGC'GCA
Cuts at: 929 1962 929
Size: 1033 3148
HaeII r_GCGC'y
Cuts at: 1615 1863 2885 3864 1615
Size: 248 1022 979 1932
Fragments arranged by size:
1932 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 298 330 400 432 2023 2053 2055
Size: 32 121 32 70 32 1591 30 2
Cuts at: 2055 2085 145
Size: 30 2241
Fragments arranged by size:
2241 1591 121 70 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2852 3036 2852
Size: 184 3997
MscI TGG'CCA
Cuts at: 1942 1942
Size: 4181
MslI CAynn'nnrTG
Cuts at: 1096 2170 2297 1096
Size: 1074 127 2980
Fragments arranged by size:
2980 1074 127
MspA1I CmG'CkG
Cuts at: 135 793 1292 1606 1966 2979 3224 135
Size: 658 499 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 658 499 360 314 245
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 1234 1734 2641 1234
Size: 500 907 2774
Fragments arranged by size:
2774 907 500
PasI CC'CwG_GG
Cuts at: 557 557
Size: 4181
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PfoI T'CCnGG_A
Cuts at: 287 584 287
Size: 297 3884
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PmlI CAC'GTG
Cuts at: 1032 1032
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 1123 1123
Size: 4181
PspOMI G'GGCC_C
Cuts at: 253 301 253
Size: 48 4133
PstI C_TGCA'G
Cuts at: 329 568 1228 1913 329
Size: 239 660 685 2597
Fragments arranged by size:
2597 685 660 239
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 1606 1966 1606
Size: 360 3821
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1040 1195 1040
Size: 155 4026
SacII CC_GC'GG
Cuts at: 136 136
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SanDI GG'GwC_CC
Cuts at: 1123 1123
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 1045 2204 2414 1045
Size: 1159 210 2812
Fragments arranged by size:
2812 1159 210
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SexAI A'CCwGG_T
Cuts at: 641 641
Size: 4181
SfcI C'TryA_G
Cuts at: 33 325 564 669 1224 1909 2902 3093 33
Size: 292 239 105 555 685 993 191 1121
Fragments arranged by size:
1121 993 685 555 292 239 191 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 309 309
Size: 4181
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 1203 1203
Size: 4181
SmlI C'TyrA_G
Cuts at: 909 1336 2251 2743 3005 909
Size: 427 915 492 262 2085
Fragments arranged by size:
2085 915 492 427 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
StuI AGG'CCT
Cuts at: 852 852
Size: 4181
StyI C'CwwG_G
Cuts at: 768 1315 1669 768
Size: 547 354 3280
Fragments arranged by size:
3280 547 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1764 1897 2356 2565 1764
Size: 133 459 209 3380
Fragments arranged by size:
3380 459 209 133
TatI w'GTAC_w
Cuts at: 734 3696 734
Size: 2962 1219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 694 709 726 741 973 1005 3698 3730 694
Size: 15 17 15 232 32 2693 32 1145
Fragments arranged by size:
2693 1145 232 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1093 1978 1093
Size: 885 3296
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 772 1676 772
Size: 904 3277
XmaI C'CCGG_G
Cuts at: 1201 1201
Size: 4181
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PshAI PsiI PspXI
PsrI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI