Restriction map for
pF1KB0928
(Circular) (Six-base) MAPSORT of: pF1KB0928.seq Check: 3847 from: 1 to: 4205
pF1KB0928 4205 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 379 379
Size: 4205
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1100 2030 2462 3209 3477 1100
Size: 930 432 747 268 1828
Fragments arranged by size:
1828 930 747 432 268
AfeI AGC'GCT
Cuts at: 758 778 758
Size: 20 4185
AflIII A'CryG_T
Cuts at: 746 1557 2661 3638 746
Size: 811 1104 977 1313
Fragments arranged by size:
1313 1104 977 811
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 852 952 967 985 1510 3077 852
Size: 100 15 18 525 1567 1980
Fragments arranged by size:
1980 1567 525 100 18 15
ApaLI G'TGCA_C
Cuts at: 226 2975 226
Size: 2749 1456
ApoI r'AATT_y
Cuts at: 1131 1209 3558 3814 1131
Size: 78 2349 256 1522
Fragments arranged by size:
2349 1522 256 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 137 568 1225 137
Size: 431 657 3117
Fragments arranged by size:
3117 657 431
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 1221 1635 1883 1918 1221
Size: 414 248 35 3508
Fragments arranged by size:
3508 414 248 35
BanII G_rGCy'C
Cuts at: 1036 1219 2249 1036
Size: 183 1030 2992
Fragments arranged by size:
2992 1030 183
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3957
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 4205
BbvCI CC'TCA_GC
Cuts at: 251 251
Size: 4205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 142 176 142
Size: 34 4171
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglI GCCn_nnn'nGGC
Cuts at: 1180 1180
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4205
Bme1580I G_kGCm'C
Cuts at: 230 617 1830 1923 2979 230
Size: 387 1213 93 1056 1456
Fragments arranged by size:
1456 1213 1056 387 93
BmrI ACTGGGnnnn_n'
Cuts at: 1051 1417 1498 1833 3977 1051
Size: 366 81 335 2144 1279
Fragments arranged by size:
2144 1279 366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 180 2572 180
Size: 2392 1813
Bpu10I CC'TnA_GC
Cuts at: 251 556 251
Size: 305 3900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2260 2752 3050 1381
Size: 879 492 298 2536
Fragments arranged by size:
2536 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 572 3909 572
Size: 3337 868
BsaAI yAC'GTr
Cuts at: 534 1558 534
Size: 1024 3181
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 1636 1884 2586 4082 1636
Size: 248 702 1496 1759
Fragments arranged by size:
1759 1496 702 248
BsaWI w'CCGG_w
Cuts at: 414 1394 1915 2593 2867 3014 3425 414
Size: 980 521 678 274 147 411 1194
Fragments arranged by size:
1194 980 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 886 916 886
Size: 30 4175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 418
Size: 4205
BseYI C'CCAG_C
Cuts at: 857 1630 2388 2965 3461 857
Size: 773 758 577 496 1601
Fragments arranged by size:
1601 773 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 357 556 256
Size: 101 199 3905
Fragments arranged by size:
3905 199 101
BsiEI CG_ry'CG
Cuts at: 104 1179 1747 1793 3001 104
Size: 1075 568 46 1208 1308
Fragments arranged by size:
1308 1208 1075 568 46
BsiHKAI G_wGCw'C
Cuts at: 230 1219 1997 2187 2979 230
Size: 989 778 190 792 1456
Fragments arranged by size:
1456 989 792 778 190
BsiWI C'GTAC_G
Cuts at: 700 749 700
Size: 49 4156
BsmI GAATG_Cn'
Cuts at: 533 533
Size: 4205
Bsp1286I G_dGCh'C
Cuts at: 230 617 1036 1219 1830 1923 1997 2187 2249
Size: 387 419 183 611 93 74 190 62
Cuts at: 2249 2979 230
Size: 730 1456
Fragments arranged by size:
1456 730 611 419 387 190 183 93
74 62
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 140 278 379 1255 1469 1771 2152 140
Size: 138 101 876 214 302 381 2193
Fragments arranged by size:
2193 876 381 302 214 138 101
BsrBI CCG'CTC
Cuts at: 50 465 587 1161 2497 2551 4035 4200 50
Size: 415 122 574 1336 54 1484 165 55
Fragments arranged by size:
1484 1336 574 415 165 122 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 551 2203 2384 2593 551
Size: 1652 181 209 2163
Fragments arranged by size:
2163 1652 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 475 985 1582 475
Size: 510 597 3098
Fragments arranged by size:
3098 597 510
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstYI r'GATC_y
Cuts at: 1 1230 1697 1720 2055 2301 3313 3399 3411
Size: 1229 467 23 335 246 1012 86 12
Cuts at: 3411 3757 1
Size: 346 449
Fragments arranged by size:
1229 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 945 945
Size: 4205
BtgI C'CryG_G
Cuts at: 107 884 107
Size: 777 3428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 485 701 812 1448 485
Size: 216 111 636 3242
Fragments arranged by size:
3242 636 216 111
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 887 1790 1964 2355 2382 3727 887
Size: 903 174 391 27 1345 1365
Fragments arranged by size:
1365 1345 903 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 175 2228 2438 175
Size: 2053 210 1942
Fragments arranged by size:
2053 1942 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2413 2723 2869 4015 2413
Size: 310 146 1146 2603
Fragments arranged by size:
2603 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1100 2030 2462 3209 3477 1100
Size: 930 432 747 268 1828
Fragments arranged by size:
1828 930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 495 1344 495
Size: 849 3356
EcoRI G'AATT_C
Cuts at: 1131 1209 1131
Size: 78 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 760 780 1639 1887 2909 3888 760
Size: 20 859 248 1022 979 1077
Fragments arranged by size:
1077 1022 979 859 248 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2047 2077 2079 2109 2047
Size: 30 2 30 4143
Fragments arranged by size:
4143 30 30 2
HincII GTy'rAC
Cuts at: 1244 1244
Size: 4205
HindIII A'AGCT_T
Cuts at: 283 1112 283
Size: 829 3376
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3957
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 746 3638 746
Size: 2892 1313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1050 2876 3060 1050
Size: 1826 184 2195
Fragments arranged by size:
2195 1826 184
MscI TGG'CCA
Cuts at: 889 1966 889
Size: 1077 3128
MslI CAynn'nnrTG
Cuts at: 304 1097 2194 2321 304
Size: 793 1097 127 2188
Fragments arranged by size:
2188 1097 793 127
MspA1I CmG'CkG
Cuts at: 250 814 820 861 964 985 1316 1630 1990
Size: 564 6 41 103 21 331 314 360
Cuts at: 1990 3003 3248 250
Size: 1013 245 1207
Fragments arranged by size:
1207 1013 564 360 331 314 245 103
41 21 6
NaeI GCC'GGC
Cuts at: 553 2386 553
Size: 1833 2372
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3957
NcoI C'CATG_G
Cuts at: 107 884 107
Size: 777 3428
NgoMIV G'CCGG_C
Cuts at: 551 2384 551
Size: 1833 2372
NspI r_CATG'y
Cuts at: 352 1258 1758 2665 352
Size: 906 500 907 1892
Fragments arranged by size:
1892 907 906 500
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PmlI CAC'GTG
Cuts at: 534 534
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 4173
PstI C_TGCA'G
Cuts at: 819 975 1002 1252 1937 819
Size: 156 27 250 685 3087
Fragments arranged by size:
3087 685 250 156 27
PvuI CG_AT'CG
Cuts at: 104 1179 104
Size: 1075 3130
PvuII CAG'CTG
Cuts at: 250 814 820 861 964 1630 1990 250
Size: 564 6 41 103 666 360 2465
Fragments arranged by size:
2465 666 564 360 103 41 6
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 146 815 971 998 1049 1248 1933 2926
Size: 113 669 156 27 51 199 685 993
Cuts at: 2926 3117 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 669 199 191 156 113
51 27
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 139 570 1227 139
Size: 431 657 3117
Fragments arranged by size:
3117 657 431
SmlI C'TyrA_G
Cuts at: 1360 2275 2767 3029 1360
Size: 915 492 262 2536
Fragments arranged by size:
2536 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
StyI C'CwwG_G
Cuts at: 107 884 1339 1693 107
Size: 777 455 354 2619
Fragments arranged by size:
2619 777 455 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 682 1788 1921 2380 2589 682
Size: 1106 133 459 209 2298
Fragments arranged by size:
2298 1106 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 886 918 3722 3754 886
Size: 32 2804 32 1337
Fragments arranged by size:
2804 1337 32 32
Tth111I GACn'n_nGTC
Cuts at: 852 2002 852
Size: 1150 3055
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1700 1700
Size: 4205
XmaI C'CCGG_G
Cuts at: 137 568 1225 137
Size: 431 657 3117
Fragments arranged by size:
3117 657 431
XmnI GAAnn'nnTTC
Cuts at: 118 118
Size: 4205
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BpmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI