Restriction map for
pF1KB0939
(Circular) (Six-base) MAPSORT of: pF1KB0939.seq Check: 740 from: 1 to: 3593
pF1KB0939 3593 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 592 592
Size: 3593
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 1418 1850 2597 2865 338
Size: 1080 432 747 268 1066
Fragments arranged by size:
1080 1066 747 432 268
AflIII A'CryG_T
Cuts at: 945 2049 3026 945
Size: 1104 977 1512
Fragments arranged by size:
1512 1104 977
AgeI A'CCGG_T
Cuts at: 1981 1981
Size: 3593
AlwNI CAG_nnn'CTG
Cuts at: 898 2465 898
Size: 1567 2026
ApaI G_GGCC'C
Cuts at: 154 154
Size: 3593
ApaLI G'TGCA_C
Cuts at: 2363 2363
Size: 3593
ApoI r'AATT_y
Cuts at: 597 2946 3202 597
Size: 2349 256 988
Fragments arranged by size:
2349 988 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 409 613 409
Size: 204 3389
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 609 1023 1271 1306 609
Size: 414 248 35 2896
Fragments arranged by size:
2896 414 248 35
BanII G_rGCy'C
Cuts at: 154 512 607 1637 154
Size: 358 95 1030 2110
Fragments arranged by size:
2110 1030 358 95
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2252 3593 1491
Size: 761 1341 1491
Fragments arranged by size:
1491 1341 761
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 705 705
Size: 3593
Bme1580I G_kGCm'C
Cuts at: 154 1218 1311 2367 154
Size: 1064 93 1056 1380
Fragments arranged by size:
1380 1064 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 318 805 886 1221 3365 318
Size: 487 81 335 2144 546
Fragments arranged by size:
2144 546 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 358 479 535 218
Size: 140 121 56 3276
Fragments arranged by size:
3276 140 121 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 769 1648 2140 2438 363
Size: 406 879 492 298 1518
Fragments arranged by size:
1518 879 492 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 114 3297 114
Size: 3183 410
BsaAI yAC'GTr
Cuts at: 946 946
Size: 3593
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 1024 1272 1974 3470 1024
Size: 248 702 1496 1147
Fragments arranged by size:
1496 1147 702 248
BsaWI w'CCGG_w
Cuts at: 782 1303 1981 2255 2402 2813 782
Size: 521 678 274 147 411 1562
Fragments arranged by size:
1562 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 547
Size: 30 3563
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 218 221 355 568 206
Size: 12 3 134 213 3231
Fragments arranged by size:
3231 213 134 12 3
BseYI C'CCAG_C
Cuts at: 470 1018 1776 2353 2849 470
Size: 548 758 577 496 1214
Fragments arranged by size:
1214 758 577 548 496
BsiEI CG_ry'CG
Cuts at: 104 1135 1181 2389 104
Size: 1031 46 1208 1308
Fragments arranged by size:
1308 1208 1031 46
BsiHKAI G_wGCw'C
Cuts at: 512 607 1385 1575 2367 512
Size: 95 778 190 792 1738
Fragments arranged by size:
1738 792 778 190 95
Bsp1286I G_dGCh'C
Cuts at: 154 512 607 1218 1311 1385 1575 1637 2367
Size: 358 95 611 93 74 190 62 730
Cuts at: 2367 154
Size: 1380
Fragments arranged by size:
1380 730 611 358 190 95 93 74
62
BspEI T'CCGG_A
Cuts at: 2813 2813
Size: 3593
BspHI T'CATG_A
Cuts at: 2769 2769
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 592 643 857 1159 1540 592
Size: 51 214 302 381 2645
Fragments arranged by size:
2645 381 302 214 51
BsrBI CCG'CTC
Cuts at: 50 266 1885 1939 3423 3588 50
Size: 216 1619 54 1484 165 55
Fragments arranged by size:
1619 1484 216 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1505 1505
Size: 3593
BsrFI r'CCGG_y
Cuts at: 1591 1772 1981 1591
Size: 181 209 3203
Fragments arranged by size:
3203 209 181
BssSI C'ACGA_G
Cuts at: 1864 2222 1864
Size: 358 3235
BstAPI GCAn_nnn'nTGC
Cuts at: 970 970
Size: 3593
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstYI r'GATC_y
Cuts at: 1 618 1085 1108 1443 1689 2701 2787 2799
Size: 617 467 23 335 246 1012 86 12
Cuts at: 2799 3145 1
Size: 346 449
Fragments arranged by size:
1012 617 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 158 158
Size: 3593
BtgI C'CryG_G
Cuts at: 107 107
Size: 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 1724 1887 1543
Size: 181 163 3249
Fragments arranged by size:
3249 181 163
BtsI GCAGTG_nn'
Cuts at: 836 836
Size: 3593
DraI TTT'AAA
Cuts at: 592 592
Size: 3593
DrdI GACnn_nn'nnGTC
Cuts at: 1299 2157 1299
Size: 858 2735
EaeI y'GGCC_r
Cuts at: 1178 1352 1743 1770 3115 1178
Size: 174 391 27 1345 1656
Fragments arranged by size:
1656 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1178 1178
Size: 3593
EarI CTCTTCn'nnn_
Cuts at: 128 1616 1826 128
Size: 1488 210 1895
Fragments arranged by size:
1895 1488 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1801 2111 2257 3403 1801
Size: 310 146 1146 1991
Fragments arranged by size:
1991 1146 310 146
EcoICRI GAG'CTC
Cuts at: 510 605 510
Size: 95 3498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 338 358 479 535 1418 1850 2597 2865
Size: 120 20 121 56 883 432 747 268
Cuts at: 2865 218
Size: 946
Fragments arranged by size:
946 883 747 432 268 121 120 56
20
EcoNI CCTnn'n_nnAGG
Cuts at: 233 233
Size: 3593
EcoO109I rG'GnC_Cy
Cuts at: 150 151 239 259 563 732 150
Size: 1 88 20 304 169 3011
Fragments arranged by size:
3011 304 169 88 20 1
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 1612
Size: 3593
FspI TGC'GCA
Cuts at: 1374 1374
Size: 3593
HaeII r_GCGC'y
Cuts at: 1027 1275 2297 3276 1027
Size: 248 1022 979 1344
Fragments arranged by size:
1344 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1435 1465 1467 1497 547
Size: 32 856 30 2 30 2643
Fragments arranged by size:
2643 856 32 30 30 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 3026 3026
Size: 3593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2448 2264
Size: 184 3409
MscI TGG'CCA
Cuts at: 1354 1354
Size: 3593
MslI CAynn'nnrTG
Cuts at: 1582 1709 1582
Size: 127 3466
MspA1I CmG'CkG
Cuts at: 459 474 704 1018 1378 2391 2636 459
Size: 15 230 314 360 1013 245 1416
Fragments arranged by size:
1416 1013 360 314 245 230 15
NaeI GCC'GGC
Cuts at: 1774 1774
Size: 3593
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NcoI C'CATG_G
Cuts at: 107 107
Size: 3593
NgoMIV G'CCGG_C
Cuts at: 1772 1772
Size: 3593
NspI r_CATG'y
Cuts at: 646 1146 2053 646
Size: 500 907 2186
Fragments arranged by size:
2186 907 500
PasI CC'CwG_GG
Cuts at: 255 255
Size: 3593
PciI A'CATG_T
Cuts at: 2049 2049
Size: 3593
PflMI CCAn_nnn'nTGG
Cuts at: 462 462
Size: 3593
PfoI T'CCnGG_A
Cuts at: 512 512
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 3561
PpuMI rG'GwC_Cy
Cuts at: 239 259 563 239
Size: 20 304 3269
Fragments arranged by size:
3269 304 20
PspOMI G'GGCC_C
Cuts at: 150 150
Size: 3593
PstI C_TGCA'G
Cuts at: 290 640 1325 290
Size: 350 685 2558
Fragments arranged by size:
2558 685 350
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3593
PvuII CAG'CTG
Cuts at: 1018 1378 1018
Size: 360 3233
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 512 607 512
Size: 95 3498
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SanDI GG'GwC_CC
Cuts at: 239 259 239
Size: 20 3573
SapI GCTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SexAI A'CCwGG_T
Cuts at: 308 308
Size: 3593
SfcI C'TryA_G
Cuts at: 33 286 636 1321 2314 2505 33
Size: 253 350 685 993 191 1121
Fragments arranged by size:
1121 993 685 350 253 191
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 615 615
Size: 3593
SmlI C'TyrA_G
Cuts at: 378 409 748 1663 2155 2417 378
Size: 31 339 915 492 262 1554
Fragments arranged by size:
1554 915 492 339 262 31
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 646 1146 646
Size: 500 3093
StyI C'CwwG_G
Cuts at: 107 202 727 1081 107
Size: 95 525 354 2619
Fragments arranged by size:
2619 525 354 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1176 1309 1768 1977 1176
Size: 133 459 209 2792
Fragments arranged by size:
2792 459 209 133
TatI w'GTAC_w
Cuts at: 3108 3108
Size: 3593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3561
Tth111I GACn'n_nGTC
Cuts at: 1390 1390
Size: 3593
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1088 1088
Size: 3593
XhoI C'TCGA_G
Cuts at: 409 409
Size: 3593
XmaI C'CCGG_G
Cuts at: 613 613
Size: 3593
XmnI GAAnn'nnTTC
Cuts at: 299 299
Size: 3593
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI