Restriction map for
pF1KB0942
(Circular) (Six-base) MAPSORT of: pF1KB0942.seq Check: 1427 from: 1 to: 4289
pF1KB0942 4289 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 2114 2546 3293 3561 475
Size: 1639 432 747 268 1203
Fragments arranged by size:
1639 1203 747 432 268
AflII C'TTAA_G
Cuts at: 934 934
Size: 4289
AflIII A'CryG_T
Cuts at: 857 997 1641 2745 3722 857
Size: 140 644 1104 977 1424
Fragments arranged by size:
1424 1104 977 644 140
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AleI CACnn'nnGTG
Cuts at: 813 813
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 253 754 769 1594 3161 253
Size: 501 15 825 1567 1381
Fragments arranged by size:
1567 1381 825 501 15
ApaLI G'TGCA_C
Cuts at: 1120 3059 1120
Size: 1939 2350
ApoI r'AATT_y
Cuts at: 373 725 739 826 872 1293 3642 3898 373
Size: 352 14 87 46 421 2349 256 764
Fragments arranged by size:
2349 764 421 352 256 87 46 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 169 1309 169
Size: 1140 3149
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 703 1305 1719 1967 2002 703
Size: 602 414 248 35 2990
Fragments arranged by size:
2990 602 414 248 35
BanII G_rGCy'C
Cuts at: 170 339 1303 2333 170
Size: 169 964 1030 2126
Fragments arranged by size:
2126 1030 964 169
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 1809 1809
Size: 4289
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 1124 1914 2007 3063 1124
Size: 790 93 1056 2350
Fragments arranged by size:
2350 1056 790 93
BmgBI CAC'GTC
Cuts at: 998 998
Size: 4289
BmrI ACTGGGnnnn_n'
Cuts at: 287 1501 1582 1917 4061 287
Size: 1214 81 335 2144 515
Fragments arranged by size:
2144 1214 515 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1034 791
Size: 243 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2344 2836 3134 1465
Size: 879 492 298 2620
Fragments arranged by size:
2620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3993 3993
Size: 4289
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 1803 3840 6
Size: 1797 2037 455
Fragments arranged by size:
2037 1797 455
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 220
Size: 30 4259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 647 647
Size: 4289
BseYI C'CCAG_C
Cuts at: 1714 2472 3049 3545 1714
Size: 758 577 496 2458
Fragments arranged by size:
2458 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1124 1303 2081 2271 3063 1124
Size: 179 778 190 792 2350
Fragments arranged by size:
2350 792 778 190 179
BsmI GAATG_Cn'
Cuts at: 454 580 454
Size: 126 4163
Bsp1286I G_dGCh'C
Cuts at: 170 339 1124 1303 1914 2007 2081 2271 2333
Size: 169 785 179 611 93 74 190 62
Cuts at: 2333 3063 170
Size: 730 1396
Fragments arranged by size:
1396 785 730 611 190 179 169 93
74 62
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 837 3465 837
Size: 2628 1661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 1272 1339 1553 1855 2236 126
Size: 1146 67 214 302 381 2179
Fragments arranged by size:
2179 1146 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 406 2581 2635 4119 4284 50
Size: 356 2175 54 1484 165 55
Fragments arranged by size:
2175 1484 356 165 55 54
BsrDI GCAATG_nn'
Cuts at: 199 2201 199
Size: 2002 2287
BsrFI r'CCGG_y
Cuts at: 2287 2468 2677 2287
Size: 181 209 3899
Fragments arranged by size:
3899 209 181
BsrGI T'GTAC_A
Cuts at: 156 156
Size: 4289
BssSI C'ACGA_G
Cuts at: 187 2560 2918 187
Size: 2373 358 1558
Fragments arranged by size:
2373 1558 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1666 1666
Size: 4289
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstEII G'GTnAC_C
Cuts at: 483 483
Size: 4289
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4289
BstYI r'GATC_y
Cuts at: 1 1314 1781 1804 2139 2385 3397 3483 3495
Size: 1313 467 23 335 246 1012 86 12
Cuts at: 3495 3841 1
Size: 346 449
Fragments arranged by size:
1313 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 230 686 770 1532 230
Size: 456 84 762 2987
Fragments arranged by size:
2987 762 456 84
DraI TTT'AAA
Cuts at: 831 1288 831
Size: 457 3832
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 1874 2048 2439 2466 3811 1874
Size: 174 391 27 1345 2352
Fragments arranged by size:
2352 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 1216 1246 2312 2522 1216
Size: 30 1066 210 2983
Fragments arranged by size:
2983 1066 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2497 2807 2953 4099 2497
Size: 310 146 1146 2687
Fragments arranged by size:
2687 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 791 1034 2114 2546 3293 3561 475
Size: 316 243 1080 432 747 268 1203
Fragments arranged by size:
1203 1080 747 432 316 268 243
EcoO109I rG'GnC_Cy
Cuts at: 138 1428 138
Size: 1290 2999
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
EcoRV GAT'ATC
Cuts at: 1211 1211
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1060 2308 1060
Size: 1248 3041
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 1723 1971 2993 3972 1723
Size: 248 1022 979 2040
Fragments arranged by size:
2040 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 676 708 2131 2161 2163 2193 676
Size: 32 1423 30 2 30 2772
Fragments arranged by size:
2772 1423 32 30 30 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
HindIII A'AGCT_T
Cuts at: 931 931
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1223 2960 3144 1223
Size: 1737 184 2368
Fragments arranged by size:
2368 1737 184
MscI TGG'CCA
Cuts at: 2050 2050
Size: 4289
MslI CAynn'nnrTG
Cuts at: 305 350 813 922 2278 2405 305
Size: 45 463 109 1356 127 2189
Fragments arranged by size:
2189 1356 463 127 109 45
MspA1I CmG'CkG
Cuts at: 1054 1400 1714 2074 3087 3332 1054
Size: 346 314 360 1013 245 2011
Fragments arranged by size:
2011 1013 360 346 314 245
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 4289
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 107 107
Size: 4289
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 4289
NspI r_CATG'y
Cuts at: 861 1342 1842 2749 861
Size: 481 500 907 2401
Fragments arranged by size:
2401 907 500 481
PciI A'CATG_T
Cuts at: 857 2745 857
Size: 1888 2401
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PpuMI rG'GwC_Cy
Cuts at: 138 138
Size: 4289
PsiI TTA'TAA
Cuts at: 787 787
Size: 4289
PstI C_TGCA'G
Cuts at: 1059 1336 2021 1059
Size: 277 685 3327
Fragments arranged by size:
3327 685 277
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 1054 1714 2074 1054
Size: 660 360 3269
Fragments arranged by size:
3269 660 360
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 1055 1332 2017 3010 3201 33
Size: 1022 277 685 993 191 1121
Fragments arranged by size:
1121 1022 993 685 277 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 1311 1311
Size: 4289
SmlI C'TyrA_G
Cuts at: 934 1444 2359 2851 3113 934
Size: 510 915 492 262 2110
Fragments arranged by size:
2110 915 510 492 262
SpeI A'CTAG_T
Cuts at: 67 810 67
Size: 743 3546
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
StyI C'CwwG_G
Cuts at: 107 1423 1777 107
Size: 1316 354 2619
Fragments arranged by size:
2619 1316 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 518 1872 2005 2464 2673 518
Size: 1354 133 459 209 2134
Fragments arranged by size:
2134 1354 459 209 133
TatI w'GTAC_w
Cuts at: 156 3804 156
Size: 3648 641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 220 252 3806 3838 220
Size: 32 3554 32 671
Fragments arranged by size:
3554 671 32 32
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1784 1784
Size: 4289
XmaI C'CCGG_G
Cuts at: 1309 1309
Size: 4289
XmnI GAAnn'nnTTC
Cuts at: 296 1043 296
Size: 747 3542
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsgI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI