Restriction map for
pF1KB0945
(Circular) (Six-base) MAPSORT of: pF1KB0945.seq Check: 6076 from: 1 to: 3623
pF1KB0945 3623 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 317 506 661 317
Size: 189 155 3279
Fragments arranged by size:
3279 189 155
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1880 2627 2895 1448
Size: 432 747 268 2176
Fragments arranged by size:
2176 747 432 268
AflIII A'CryG_T
Cuts at: 975 2079 3056 975
Size: 1104 977 1542
Fragments arranged by size:
1542 1104 977
AgeI A'CCGG_T
Cuts at: 2011 2011
Size: 3623
AlwNI CAG_nnn'CTG
Cuts at: 928 2495 928
Size: 1567 2056
ApaLI G'TGCA_C
Cuts at: 2393 2393
Size: 3623
ApoI r'AATT_y
Cuts at: 328 562 627 2976 3232 328
Size: 234 65 2349 256 719
Fragments arranged by size:
2349 719 256 234 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 643 643
Size: 3623
BamHI G'GATC_C
Cuts at: 648 648
Size: 3623
BanI G'GyrC_C
Cuts at: 639 1053 1301 1336 639
Size: 414 248 35 2926
Fragments arranged by size:
2926 414 248 35
BanII G_rGCy'C
Cuts at: 637 1667 637
Size: 1030 2593
BbeI G_GCGC'C
Cuts at: 1057 1305 1057
Size: 248 3375
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2282 3623 1521
Size: 761 1341 1521
Fragments arranged by size:
1521 1341 761
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 735 735
Size: 3623
Bme1580I G_kGCm'C
Cuts at: 1248 1341 2397 1248
Size: 93 1056 2474
Fragments arranged by size:
2474 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 835 916 1251 3395 835
Size: 81 335 2144 1063
Fragments arranged by size:
2144 1063 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 360 1990 360
Size: 1630 1993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1678 2170 2468 799
Size: 879 492 298 1954
Fragments arranged by size:
1954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 3623
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 1137 3174 6
Size: 1131 2037 455
Fragments arranged by size:
2037 1131 455
BsaHI Gr'CG_yC
Cuts at: 341 1054 1302 2004 3500 341
Size: 713 248 702 1496 464
Fragments arranged by size:
1496 713 702 464 248
BsaWI w'CCGG_w
Cuts at: 812 1333 2011 2285 2432 2843 812
Size: 521 678 274 147 411 1592
Fragments arranged by size:
1592 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1048 1806 2383 2879 1048
Size: 758 577 496 1792
Fragments arranged by size:
1792 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1165 1211 2419 104
Size: 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 46
BsiHKAI G_wGCw'C
Cuts at: 145 637 1415 1605 2397 145
Size: 492 778 190 792 1371
Fragments arranged by size:
1371 792 778 492 190
BsmBI CGTCTCn'nnnn_
Cuts at: 348 348
Size: 3623
Bsp1286I G_dGCh'C
Cuts at: 145 637 1248 1341 1415 1605 1667 2397 145
Size: 492 611 93 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 492 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2843 2843
Size: 3623
BspHI T'CATG_A
Cuts at: 2799 2799
Size: 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 673 887 1189 1570 673
Size: 214 302 381 2726
Fragments arranged by size:
2726 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1915 1969 3453 3618 50
Size: 1865 54 1484 165 55
Fragments arranged by size:
1865 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1535 1535
Size: 3623
BsrFI r'CCGG_y
Cuts at: 1621 1802 2011 1621
Size: 181 209 3233
Fragments arranged by size:
3233 209 181
BssSI C'ACGA_G
Cuts at: 1894 2252 1894
Size: 358 3265
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1000
Size: 3623
BstBI TT'CG_AA
Cuts at: 1984 1984
Size: 3623
BstYI r'GATC_y
Cuts at: 1 648 1115 1138 1473 1719 2731 2817 2829
Size: 647 467 23 335 246 1012 86 12
Cuts at: 2829 3175 1
Size: 346 449
Fragments arranged by size:
1012 647 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 1754 1917 1573
Size: 181 163 3279
Fragments arranged by size:
3279 181 163
BtsI GCAGTG_nn'
Cuts at: 866 866
Size: 3623
DraI TTT'AAA
Cuts at: 324 622 324
Size: 298 3325
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2187 1329
Size: 858 2765
EaeI y'GGCC_r
Cuts at: 1208 1382 1773 1800 3145 1208
Size: 174 391 27 1345 1686
Fragments arranged by size:
1686 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1208 1208
Size: 3623
EarI CTCTTCn'nnn_
Cuts at: 224 448 1646 1856 224
Size: 224 1198 210 1991
Fragments arranged by size:
1991 1198 224 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 2141 2287 3433 1831
Size: 310 146 1146 2021
Fragments arranged by size:
2021 1146 310 146
EcoICRI GAG'CTC
Cuts at: 635 635
Size: 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1880 2627 2895 1448
Size: 432 747 268 2176
Fragments arranged by size:
2176 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 762 762
Size: 3623
EcoRI G'AATT_C
Cuts at: 328 562 627 328
Size: 234 65 3324
Fragments arranged by size:
3324 234 65
EcoRV GAT'ATC
Cuts at: 591 591
Size: 3623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 146 1642 146
Size: 1496 2127
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 1057 1305 2327 3306 1057
Size: 248 1022 979 1374
Fragments arranged by size:
1374 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 1465 1495 1497 1527 223
Size: 32 1210 30 2 30 2319
Fragments arranged by size:
2319 1210 32 30 30 2
HincII GTy'rAC
Cuts at: 662 662
Size: 3623
HindIII A'AGCT_T
Cuts at: 193 193
Size: 3623
KasI G'GCGC_C
Cuts at: 1053 1301 1053
Size: 248 3375
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2478 2294
Size: 184 3439
MscI TGG'CCA
Cuts at: 1384 1384
Size: 3623
MslI CAynn'nnrTG
Cuts at: 152 1612 1739 152
Size: 1460 127 2036
Fragments arranged by size:
2036 1460 127
MspA1I CmG'CkG
Cuts at: 734 1048 1408 2421 2666 734
Size: 314 360 1013 245 1691
Fragments arranged by size:
1691 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1804 1804
Size: 3623
NarI GG'CG_CC
Cuts at: 1054 1302 1054
Size: 248 3375
NcoI C'CATG_G
Cuts at: 107 107
Size: 3623
NgoMIV G'CCGG_C
Cuts at: 1802 1802
Size: 3623
NspI r_CATG'y
Cuts at: 676 1176 2083 676
Size: 500 907 2216
Fragments arranged by size:
2216 907 500
PciI A'CATG_T
Cuts at: 2079 2079
Size: 3623
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 3591
PstI C_TGCA'G
Cuts at: 670 1355 670
Size: 685 2938
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 1048 1408 1048
Size: 360 3263
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 637 637
Size: 3623
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 1646 1856 1646
Size: 210 3413
SbfI CC_TGCA'GG
Cuts at: 670 670
Size: 3623
ScaI AGT'ACT
Cuts at: 3140 3140
Size: 3623
SfcI C'TryA_G
Cuts at: 33 666 1351 2344 2535 33
Size: 633 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 191
SfoI GGC'GCC
Cuts at: 1055 1303 1055
Size: 248 3375
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SmaI CCC'GGG
Cuts at: 645 645
Size: 3623
SmlI C'TyrA_G
Cuts at: 778 1693 2185 2447 778
Size: 915 492 262 1954
Fragments arranged by size:
1954 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 107 757 1111 107
Size: 650 354 2619
Fragments arranged by size:
2619 650 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 1339 1798 2007 1206
Size: 133 459 209 2822
Fragments arranged by size:
2822 459 209 133
TatI w'GTAC_w
Cuts at: 270 3138 270
Size: 2868 755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3591
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1118 1118
Size: 3623
XmaI C'CCGG_G
Cuts at: 643 643
Size: 3623
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI