Restriction map for
pF1KB0948
(Circular) (Six-base) MAPSORT of: pF1KB0948.seq Check: 7172 from: 1 to: 4319
pF1KB0948 4319 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 324 1357 324
Size: 1033 3286
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2576 3323 3591 2144
Size: 432 747 268 2872
Fragments arranged by size:
2872 747 432 268
AflII C'TTAA_G
Cuts at: 1008 1008
Size: 4319
AflIII A'CryG_T
Cuts at: 319 1671 2775 3752 319
Size: 1352 1104 977 886
Fragments arranged by size:
1352 1104 977 886
AgeI A'CCGG_T
Cuts at: 2707 2707
Size: 4319
AlwNI CAG_nnn'CTG
Cuts at: 1154 1624 3191 1154
Size: 470 1567 2282
Fragments arranged by size:
2282 1567 470
ApaLI G'TGCA_C
Cuts at: 3089 3089
Size: 4319
ApoI r'AATT_y
Cuts at: 1323 3672 3928 1323
Size: 2349 256 1714
Fragments arranged by size:
2349 1714 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 1339 1339
Size: 4319
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 132 1335 1749 1997 2032 132
Size: 1203 414 248 35 2419
Fragments arranged by size:
2419 1203 414 248 35
BanII G_rGCy'C
Cuts at: 1333 2363 1333
Size: 1030 3289
BbeI G_GCGC'C
Cuts at: 136 1753 2001 136
Size: 1617 248 2454
Fragments arranged by size:
2454 1617 248
BbsI GAAGACnn'nnnn_
Cuts at: 190 190
Size: 4319
BciVI GTATCCnnnnn_n'
Cuts at: 2217 2978 4319 2217
Size: 761 1341 2217
Fragments arranged by size:
2217 1341 761
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BfrBI ATG'CAT
Cuts at: 1232 1232
Size: 4319
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4319
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 1431 1431
Size: 4319
Bme1580I G_kGCm'C
Cuts at: 1944 2037 3093 1944
Size: 93 1056 3170
Fragments arranged by size:
3170 1056 93
BmgBI CAC'GTC
Cuts at: 234 234
Size: 4319
BmrI ACTGGGnnnn_n'
Cuts at: 551 1531 1612 1947 4091 551
Size: 980 81 335 2144 779
Fragments arranged by size:
2144 980 779 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 262 479 731 815 831 1067 1148 1164
Size: 57 217 252 84 16 236 81 16
Cuts at: 1164 1232 205
Size: 68 3292
Fragments arranged by size:
3292 252 236 217 84 81 68 57
16 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 885 1218 1495 2374 2866 3164 885
Size: 333 277 879 492 298 2040
Fragments arranged by size:
2040 879 492 333 298 277
BsaI GGTCTCn'nnnn_
Cuts at: 238 4023 238
Size: 3785 534
BsaAI yAC'GTr
Cuts at: 320 1672 320
Size: 1352 2967
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 133 1750 1998 2700 4196 133
Size: 1617 248 702 1496 256
Fragments arranged by size:
1617 1496 702 256 248
BsaWI w'CCGG_w
Cuts at: 1508 2029 2707 2981 3128 3539 1508
Size: 521 678 274 147 411 2288
Fragments arranged by size:
2288 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1744 2502 3079 3575 1744
Size: 758 577 496 2488
Fragments arranged by size:
2488 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 582 834 1167 498
Size: 84 252 333 3650
Fragments arranged by size:
3650 333 252 84
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3115 104
Size: 1757 46 1208 1308
Fragments arranged by size:
1757 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 319 1333 2111 2301 3093 319
Size: 1014 778 190 792 1545
Fragments arranged by size:
1545 1014 792 778 190
BsmI GAATG_Cn'
Cuts at: 733 901 1234 733
Size: 168 333 3818
Fragments arranged by size:
3818 333 168
Bsp1286I G_dGCh'C
Cuts at: 319 1333 1944 2037 2111 2301 2363 3093 319
Size: 1014 611 93 74 190 62 730 1545
Fragments arranged by size:
1545 1014 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3539 3539
Size: 4319
BspHI T'CATG_A
Cuts at: 525 3495 525
Size: 2970 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1369 1583 1885 2266 1369
Size: 214 302 381 3422
Fragments arranged by size:
3422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 124 2611 2665 4149 4314 50
Size: 74 2487 54 1484 165 55
Fragments arranged by size:
2487 1484 165 74 55 54
BsrDI GCAATG_nn'
Cuts at: 738 2231 738
Size: 1493 2826
BsrFI r'CCGG_y
Cuts at: 2317 2498 2707 2317
Size: 181 209 3929
Fragments arranged by size:
3929 209 181
BssSI C'ACGA_G
Cuts at: 2590 2948 2590
Size: 358 3961
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 1696
Size: 4319
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstYI r'GATC_y
Cuts at: 1 829 1344 1811 1834 2169 2415 3427 3513
Size: 828 515 467 23 335 246 1012 86
Cuts at: 3513 3525 3871 1
Size: 12 346 449
Fragments arranged by size:
1012 828 515 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 325 325
Size: 4319
BtgI C'CryG_G
Cuts at: 107 107
Size: 4319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 153 570 822 1075 1155 1562 153
Size: 417 252 253 80 407 2910
Fragments arranged by size:
2910 417 407 253 252 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 720 755 720
Size: 35 4284
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 2883 2025
Size: 858 3461
EaeI y'GGCC_r
Cuts at: 1904 2078 2469 2496 3841 1904
Size: 174 391 27 1345 2382
Fragments arranged by size:
2382 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 423 600 936 2342 2552 423
Size: 177 336 1406 210 2190
Fragments arranged by size:
2190 1406 336 210 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 2837 2983 4129 2527
Size: 310 146 1146 2717
Fragments arranged by size:
2717 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 262 479 731 815 831 1067 1148 1164
Size: 57 217 252 84 16 236 81 16
Cuts at: 1164 1232 2144 2576 3323 3591 205
Size: 68 912 432 747 268 933
Fragments arranged by size:
933 912 747 432 268 252 236 217
84 81 68 57 16 16
EcoO109I rG'GnC_Cy
Cuts at: 1458 1458
Size: 4319
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 4319
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 136 1753 2001 3023 4002 136
Size: 1617 248 1022 979 453
Fragments arranged by size:
1617 1022 979 453 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2161 2191 2193 2223 2161
Size: 30 2 30 4257
Fragments arranged by size:
4257 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 132 1749 1997 132
Size: 1617 248 2454
Fragments arranged by size:
2454 1617 248
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1040 2990 3174 1040
Size: 1950 184 2185
Fragments arranged by size:
2185 1950 184
MscI TGG'CCA
Cuts at: 2080 2080
Size: 4319
MslI CAynn'nnrTG
Cuts at: 446 524 1227 2308 2435 446
Size: 78 703 1081 127 2330
Fragments arranged by size:
2330 1081 703 127 78
MspA1I CmG'CkG
Cuts at: 1430 1744 2104 3117 3362 1430
Size: 314 360 1013 245 2387
Fragments arranged by size:
2387 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 133 1750 1998 133
Size: 1617 248 2454
Fragments arranged by size:
2454 1617 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4319
NdeI CA'TA_TG
Cuts at: 1224 1224
Size: 4319
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NsiI A_TGCA'T
Cuts at: 1234 1234
Size: 4319
NspI r_CATG'y
Cuts at: 1372 1872 2779 1372
Size: 500 907 2912
Fragments arranged by size:
2912 907 500
PasI CC'CwG_GG
Cuts at: 267 267
Size: 4319
PciI A'CATG_T
Cuts at: 2775 2775
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PmlI CAC'GTG
Cuts at: 320 320
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4287
PsiI TTA'TAA
Cuts at: 1310 1310
Size: 4319
PstI C_TGCA'G
Cuts at: 1366 2051 1366
Size: 685 3634
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 1744 2104 1744
Size: 360 3959
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 600 936 2342 2552 600
Size: 336 1406 210 2367
Fragments arranged by size:
2367 1406 336 210
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 1362 2047 3040 3231 33
Size: 1329 685 993 191 1121
Fragments arranged by size:
1329 1121 993 685 191
SfoI GGC'GCC
Cuts at: 134 1751 1999 134
Size: 1617 248 2454
Fragments arranged by size:
2454 1617 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 864 1008 1197 1474 2389 2881 3143 864
Size: 144 189 277 915 492 262 2040
Fragments arranged by size:
2040 915 492 277 262 189 144
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StuI AGG'CCT
Cuts at: 366 468 636 804 888 972 1137 1305 366
Size: 102 168 168 84 84 165 168 3380
Fragments arranged by size:
3380 168 168 168 165 102 84 84
StyI C'CwwG_G
Cuts at: 107 1453 1807 107
Size: 1346 354 2619
Fragments arranged by size:
2619 1346 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1902 2035 2494 2703 1902
Size: 133 459 209 3518
Fragments arranged by size:
3518 459 209 133
TatI w'GTAC_w
Cuts at: 3834 3834
Size: 4319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4287
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1814 1814
Size: 4319
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
XmnI GAAnn'nnTTC
Cuts at: 930 930
Size: 4319
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PmeI
PmlI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmtI Bpu10I BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PflMI PfoI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI