Restriction map for
pF1KB0949
(Circular) (Six-base) MAPSORT of: pF1KB0949.seq Check: 124 from: 1 to: 3653
pF1KB0949 3653 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 647 3493 647
Size: 2846 807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1478 1910 2657 2925 283
Size: 1195 432 747 268 1011
Fragments arranged by size:
1195 1011 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 958 2525 958
Size: 1567 2086
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 673 673
Size: 3653
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 111 163 669 1083 1331 1366 111
Size: 52 506 414 248 35 2398
Fragments arranged by size:
2398 506 414 248 52 35
BanII G_rGCy'C
Cuts at: 361 667 1697 361
Size: 306 1030 2317
Fragments arranged by size:
2317 1030 306
BbeI G_GCGC'C
Cuts at: 115 167 1087 1335 115
Size: 52 920 248 2433
Fragments arranged by size:
2433 920 248 52
BbsI GAAGACnn'nnnn_
Cuts at: 577 577
Size: 3653
BciVI GTATCCnnnnn_n'
Cuts at: 533 1551 2312 3653 533
Size: 1018 761 1341 533
Fragments arranged by size:
1341 1018 761 533
BclI T'GATC_A
Cuts at: 401 1173 401
Size: 772 2881
BglI GCCn_nnn'nGGC
Cuts at: 161 161
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 1278 1371 2427 1278
Size: 93 1056 2504
Fragments arranged by size:
2504 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 310 865 946 1281 3425 310
Size: 555 81 335 2144 538
Fragments arranged by size:
2144 555 538 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 165
Size: 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 112 136 164 1084 1332 2034 3530 112
Size: 24 28 920 248 702 1496 235
Fragments arranged by size:
1496 920 702 248 235 28 24
BsaWI w'CCGG_w
Cuts at: 842 1363 2041 2315 2462 2873 842
Size: 521 678 274 147 411 1622
Fragments arranged by size:
1622 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 495 520 544 424
Size: 71 25 24 3533
Fragments arranged by size:
3533 71 25 24
BseYI C'CCAG_C
Cuts at: 368 1078 1836 2413 2909 368
Size: 710 758 577 496 1112
Fragments arranged by size:
1112 758 710 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 347
Size: 3653
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 361 667 1445 1635 2427 361
Size: 306 778 190 792 1587
Fragments arranged by size:
1587 792 778 306 190
BsmBI CGTCTCn'nnnn_
Cuts at: 276 276
Size: 3653
Bsp1286I G_dGCh'C
Cuts at: 361 667 1278 1371 1445 1635 1697 2427 361
Size: 306 611 93 74 190 62 730 1587
Fragments arranged by size:
1587 730 611 306 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 525 1945 1999 3483 3648 50
Size: 475 1420 54 1484 165 55
Fragments arranged by size:
1484 1420 475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 443 1565 443
Size: 1122 2531
BsrFI r'CCGG_y
Cuts at: 336 1651 1832 2041 336
Size: 1315 181 209 1948
Fragments arranged by size:
1948 1315 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 126 107
Size: 19 3634
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 612 652 612
Size: 40 3613
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 194 1238 1412 1803 1830 3175 194
Size: 1044 174 391 27 1345 672
Fragments arranged by size:
1345 1044 672 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 248 472 1676 1886 248
Size: 224 1204 210 2015
Fragments arranged by size:
2015 1204 224 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 552 1861 2171 2317 3463 552
Size: 1309 310 146 1146 742
Fragments arranged by size:
1309 1146 742 310 146
EcoICRI GAG'CTC
Cuts at: 359 665 359
Size: 306 3347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 283 1478 1910 2657 2925 165
Size: 118 1195 432 747 268 893
Fragments arranged by size:
1195 893 747 432 268 118
EcoO109I rG'GnC_Cy
Cuts at: 792 792
Size: 3653
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 115 167 400 1087 1335 2357 3336 115
Size: 52 233 687 248 1022 979 432
Fragments arranged by size:
1022 979 687 432 248 233 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 111 163 1083 1331 111
Size: 52 920 248 2433
Fragments arranged by size:
2433 920 248 52
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 190 409 1642 1769 190
Size: 219 1233 127 2074
Fragments arranged by size:
2074 1233 219 127
MspA1I CmG'CkG
Cuts at: 128 764 1078 1438 2451 2696 128
Size: 636 314 360 1013 245 1085
Fragments arranged by size:
1085 1013 636 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 112 164 1084 1332 112
Size: 52 920 248 2433
Fragments arranged by size:
2433 920 248 52
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PfoI T'CCnGG_A
Cuts at: 213 213
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PstI C_TGCA'G
Cuts at: 472 700 1385 472
Size: 228 685 2740
Fragments arranged by size:
2740 685 228
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 361 667 361
Size: 306 3347
SacII CC_GC'GG
Cuts at: 129 129
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 468 696 1381 2374 2565 33
Size: 435 228 685 993 191 1121
Fragments arranged by size:
1121 993 685 435 228 191
SfoI GGC'GCC
Cuts at: 113 165 1085 1333 113
Size: 52 920 248 2433
Fragments arranged by size:
2433 920 248 52
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 107 787 1141 107
Size: 680 354 2619
Fragments arranged by size:
2619 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 372 1236 1369 1828 2037 372
Size: 864 133 459 209 1988
Fragments arranged by size:
1988 864 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI