Restriction map for
pF1KB0951
(Circular) (Six-base) MAPSORT of: pF1KB0951.seq Check: 6852 from: 1 to: 3662
pF1KB0951 3662 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1487 1919 2666 2934 1487
Size: 432 747 268 2215
Fragments arranged by size:
2215 747 432 268
AflIII A'CryG_T
Cuts at: 1014 2118 3095 1014
Size: 1104 977 1581
Fragments arranged by size:
1581 1104 977
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 181
Size: 32 3630
AlwNI CAG_nnn'CTG
Cuts at: 967 2534 967
Size: 1567 2095
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 621 666 3015 3271 621
Size: 45 2349 256 1012
Fragments arranged by size:
2349 1012 256 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 437 557 682 437
Size: 120 125 3417
Fragments arranged by size:
3417 125 120
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 138 171 138
Size: 33 3629
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 441 678 1092 1340 1375 441
Size: 237 414 248 35 2728
Fragments arranged by size:
2728 414 248 237 35
BanII G_rGCy'C
Cuts at: 676 1706 676
Size: 1030 2632
BbeI G_GCGC'C
Cuts at: 1096 1344 1096
Size: 248 3414
BbsI GAAGACnn'nnnn_
Cuts at: 169 169
Size: 3662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 622 656 90
Size: 34 498 34 3096
Fragments arranged by size:
3096 498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 139 1560 2321 3662 139
Size: 1421 761 1341 139
Fragments arranged by size:
1421 1341 761 139
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglII A'GATC_T
Cuts at: 471 1177 3214 471
Size: 706 2037 919
Fragments arranged by size:
2037 919 706
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 1287 1380 2436 1287
Size: 93 1056 2513
Fragments arranged by size:
2513 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 874 955 1290 3434 874
Size: 81 335 2144 1102
Fragments arranged by size:
2144 1102 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 464
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 838 1717 2209 2507 560
Size: 278 879 492 298 1715
Fragments arranged by size:
1715 879 492 298 278
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 1093 1341 2043 3539 1093
Size: 248 702 1496 1216
Fragments arranged by size:
1496 1216 702 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1087 1845 2422 2918 1087
Size: 758 577 496 1831
Fragments arranged by size:
1831 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1204 1250 2458 104
Size: 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 46
BsiHKAI G_wGCw'C
Cuts at: 676 1454 1644 2436 676
Size: 778 190 792 1902
Fragments arranged by size:
1902 792 778 190
BsmI GAATG_Cn'
Cuts at: 311 311
Size: 3662
Bsp1286I G_dGCh'C
Cuts at: 676 1287 1380 1454 1644 1706 2436 676
Size: 611 93 74 190 62 730 1902
Fragments arranged by size:
1902 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 2838 2838
Size: 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 926 1228 1609 712
Size: 214 302 381 2765
Fragments arranged by size:
2765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1574 1574
Size: 3662
BsrFI r'CCGG_y
Cuts at: 1660 1841 2050 1660
Size: 181 209 3272
Fragments arranged by size:
3272 209 181
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstYI r'GATC_y
Cuts at: 1 471 687 1154 1177 1512 1758 2770 2856
Size: 470 216 467 23 335 246 1012 86
Cuts at: 2856 2868 3214 1
Size: 12 346 449
Fragments arranged by size:
1012 470 467 449 346 335 246 216
86 23 12
BtgI C'CryG_G
Cuts at: 342 426 342
Size: 84 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 905 905
Size: 3662
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 1247 1421 1812 1839 3184 1247
Size: 174 391 27 1345 1725
Fragments arranged by size:
1725 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 2180 2326 3472 1870
Size: 310 146 1146 2060
Fragments arranged by size:
2060 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 1487 1919 2666 2934 464
Size: 1023 432 747 268 1192
Fragments arranged by size:
1192 1023 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 801 801
Size: 3662
EcoRI G'AATT_C
Cuts at: 621 666 621
Size: 45 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 1096 1344 2366 3345 1096
Size: 248 1022 979 1413
Fragments arranged by size:
1413 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 234 266 1504 1534 1536 1566 234
Size: 32 1238 30 2 30 2330
Fragments arranged by size:
2330 1238 32 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 1092 1340 1092
Size: 248 3414
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2517 2333
Size: 184 3478
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 347 1651 1778 347
Size: 1304 127 2231
Fragments arranged by size:
2231 1304 127
MspA1I CmG'CkG
Cuts at: 152 570 773 1087 1447 2460 2705 152
Size: 418 203 314 360 1013 245 1109
Fragments arranged by size:
1109 1013 418 360 314 245 203
NaeI GCC'GGC
Cuts at: 1843 1843
Size: 3662
NarI GG'CG_CC
Cuts at: 1093 1341 1093
Size: 248 3414
NcoI C'CATG_G
Cuts at: 342 426 342
Size: 84 3578
NgoMIV G'CCGG_C
Cuts at: 1841 1841
Size: 3662
NspI r_CATG'y
Cuts at: 715 1215 2122 715
Size: 500 907 2255
Fragments arranged by size:
2255 907 500
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PflMI CCAn_nnn'nTGG
Cuts at: 654 654
Size: 3662
PfoI T'CCnGG_A
Cuts at: 599 599
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PstI C_TGCA'G
Cuts at: 709 1394 709
Size: 685 2977
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 570 1087 1447 570
Size: 517 360 2785
Fragments arranged by size:
2785 517 360
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SfcI C'TryA_G
Cuts at: 33 123 218 705 1390 2383 2574 33
Size: 90 95 487 685 993 191 1121
Fragments arranged by size:
1121 993 685 487 191 95 90
SfoI GGC'GCC
Cuts at: 1094 1342 1094
Size: 248 3414
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 439 684 439
Size: 245 3417
SmlI C'TyrA_G
Cuts at: 557 575 817 1732 2224 2486 557
Size: 18 242 915 492 262 1733
Fragments arranged by size:
1733 915 492 262 242 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StyI C'CwwG_G
Cuts at: 342 426 796 1150 342
Size: 84 370 354 2854
Fragments arranged by size:
2854 370 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1245 1378 1837 2046 1245
Size: 133 459 209 2861
Fragments arranged by size:
2861 459 209 133
TatI w'GTAC_w
Cuts at: 305 3177 305
Size: 2872 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 1459 1459
Size: 3662
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1157 1157
Size: 3662
XhoI C'TCGA_G
Cuts at: 557 557
Size: 3662
XmaI C'CCGG_G
Cuts at: 437 682 437
Size: 245 3417
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI