Restriction map for
pF1KB0955
(Circular) (Six-base) MAPSORT of: pF1KB0955.seq Check: 4809 from: 1 to: 3674
pF1KB0955 3674 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 712 712
Size: 3674
Acc65I G'GTAC_C
Cuts at: 690 690
Size: 3674
AclI AA'CG_TT
Cuts at: 3514 3514
Size: 3674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1499 1931 2678 2946 1499
Size: 432 747 268 2227
Fragments arranged by size:
2227 747 432 268
AflIII A'CryG_T
Cuts at: 1026 2130 3107 1026
Size: 1104 977 1593
Fragments arranged by size:
1593 1104 977
AgeI A'CCGG_T
Cuts at: 2062 2062
Size: 3674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3674
AlwNI CAG_nnn'CTG
Cuts at: 979 2546 979
Size: 1567 2107
ApaI G_GGCC'C
Cuts at: 255 344 255
Size: 89 3585
ApaLI G'TGCA_C
Cuts at: 2444 2444
Size: 3674
ApoI r'AATT_y
Cuts at: 409 678 3027 3283 409
Size: 269 2349 256 800
Fragments arranged by size:
2349 800 269 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
AvaI C'yCGr_G
Cuts at: 262 694 262
Size: 432 3242
BamHI G'GATC_C
Cuts at: 699 699
Size: 3674
BanI G'GyrC_C
Cuts at: 309 463 690 1104 1352 1387 309
Size: 154 227 414 248 35 2596
Fragments arranged by size:
2596 414 248 227 154 35
BanII G_rGCy'C
Cuts at: 255 344 688 1718 255
Size: 89 344 1030 2211
Fragments arranged by size:
2211 1030 344 89
BbeI G_GCGC'C
Cuts at: 313 1108 1356 313
Size: 795 248 2631
Fragments arranged by size:
2631 795 248
BbsI GAAGACnn'nnnn_
Cuts at: 571 571
Size: 3674
BciVI GTATCCnnnnn_n'
Cuts at: 1572 2333 3674 1572
Size: 761 1341 1572
Fragments arranged by size:
1572 1341 761
BclI T'GATC_A
Cuts at: 576 1194 576
Size: 618 3056
BfrBI ATG'CAT
Cuts at: 555 555
Size: 3674
BglII A'GATC_T
Cuts at: 1189 3226 1189
Size: 2037 1637
BlpI GC'TnA_GC
Cuts at: 786 786
Size: 3674
Bme1580I G_kGCm'C
Cuts at: 255 344 1299 1392 2448 255
Size: 89 955 93 1056 1481
Fragments arranged by size:
1481 1056 955 93 89
BmrI ACTGGGnnnn_n'
Cuts at: 429 886 967 1302 3446 429
Size: 457 81 335 2144 657
Fragments arranged by size:
2144 657 457 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 3674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1729 2221 2519 850
Size: 879 492 298 2005
Fragments arranged by size:
2005 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3378 3378
Size: 3674
BsaAI yAC'GTr
Cuts at: 1027 1027
Size: 3674
BsaBI GATnn'nnATC
Cuts at: 6 1188 3225 6
Size: 1182 2037 455
Fragments arranged by size:
2037 1182 455
BsaHI Gr'CG_yC
Cuts at: 310 1105 1353 2055 3551 310
Size: 795 248 702 1496 433
Fragments arranged by size:
1496 795 702 433 248
BsaWI w'CCGG_w
Cuts at: 863 1384 2062 2336 2483 2894 863
Size: 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 298 301 304 463 280
Size: 18 3 3 159 3491
Fragments arranged by size:
3491 159 18 3 3
BseYI C'CCAG_C
Cuts at: 296 349 1099 1857 2434 2930 296
Size: 53 750 758 577 496 1040
Fragments arranged by size:
1040 758 750 577 496 53
BsiEI CG_ry'CG
Cuts at: 104 1216 1262 2470 104
Size: 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 46
BsiHKAI G_wGCw'C
Cuts at: 688 1466 1656 2448 688
Size: 778 190 792 1914
Fragments arranged by size:
1914 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 255 344 688 1299 1392 1466 1656 1718 2448
Size: 89 344 611 93 74 190 62 730
Cuts at: 2448 255
Size: 1481
Fragments arranged by size:
1481 730 611 344 190 93 89 74
62
BspEI T'CCGG_A
Cuts at: 2894 2894
Size: 3674
BspHI T'CATG_A
Cuts at: 2850 2850
Size: 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 724 938 1240 1621 164
Size: 560 214 302 381 2217
Fragments arranged by size:
2217 560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 487 1966 2020 3504 3669 50
Size: 437 1479 54 1484 165 55
Fragments arranged by size:
1484 1479 437 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1586 1586
Size: 3674
BsrFI r'CCGG_y
Cuts at: 466 1672 1853 2062 466
Size: 1206 181 209 2078
Fragments arranged by size:
2078 1206 209 181
BssSI C'ACGA_G
Cuts at: 1945 2303 1945
Size: 358 3316
BstAPI GCAn_nnn'nTGC
Cuts at: 1051 1051
Size: 3674
BstBI TT'CG_AA
Cuts at: 2035 2035
Size: 3674
BstEII G'GTnAC_C
Cuts at: 184 184
Size: 3674
BstYI r'GATC_y
Cuts at: 1 699 1166 1189 1524 1770 2782 2868 2880
Size: 698 467 23 335 246 1012 86 12
Cuts at: 2880 3226 1
Size: 346 449
Fragments arranged by size:
1012 698 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 1805 1968 1624
Size: 181 163 3330
Fragments arranged by size:
3330 181 163
BtsI GCAGTG_nn'
Cuts at: 156 917 156
Size: 761 2913
DraI TTT'AAA
Cuts at: 673 673
Size: 3674
DrdI GACnn_nn'nnGTC
Cuts at: 1380 2238 1380
Size: 858 2816
EaeI y'GGCC_r
Cuts at: 1259 1433 1824 1851 3196 1259
Size: 174 391 27 1345 1737
Fragments arranged by size:
1737 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1259 1259
Size: 3674
EarI CTCTTCn'nnn_
Cuts at: 137 440 590 1697 1907 137
Size: 303 150 1107 210 1904
Fragments arranged by size:
1904 1107 303 210 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2192 2338 3484 1882
Size: 310 146 1146 2072
Fragments arranged by size:
2072 1146 310 146
EcoICRI GAG'CTC
Cuts at: 686 686
Size: 3674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1499 1931 2678 2946 1499
Size: 432 747 268 2227
Fragments arranged by size:
2227 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 251 813 251
Size: 562 3112
EcoRI G'AATT_C
Cuts at: 678 678
Size: 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1693 1693
Size: 3674
FspI TGC'GCA
Cuts at: 1455 1455
Size: 3674
HaeII r_GCGC'y
Cuts at: 313 1108 1356 2378 3357 313
Size: 795 248 1022 979 630
Fragments arranged by size:
1022 979 795 630 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1516 1546 1548 1578 1516
Size: 30 2 30 3612
Fragments arranged by size:
3612 30 30 2
HincII GTy'rAC
Cuts at: 713 713
Size: 3674
KasI G'GCGC_C
Cuts at: 309 1104 1352 309
Size: 795 248 2631
Fragments arranged by size:
2631 795 248
KpnI G_GTAC'C
Cuts at: 694 694
Size: 3674
MluI A'CGCG_T
Cuts at: 3107 3107
Size: 3674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 2345 2529 92
Size: 2253 184 1237
Fragments arranged by size:
2253 1237 184
MscI TGG'CCA
Cuts at: 1435 1435
Size: 3674
MslI CAynn'nnrTG
Cuts at: 1663 1790 1663
Size: 127 3547
MspA1I CmG'CkG
Cuts at: 212 248 785 1099 1459 2472 2717 212
Size: 36 537 314 360 1013 245 1169
Fragments arranged by size:
1169 1013 537 360 314 245 36
NaeI GCC'GGC
Cuts at: 468 1855 468
Size: 1387 2287
NarI GG'CG_CC
Cuts at: 310 1105 1353 310
Size: 795 248 2631
Fragments arranged by size:
2631 795 248
NgoMIV G'CCGG_C
Cuts at: 466 1853 466
Size: 1387 2287
NsiI A_TGCA'T
Cuts at: 557 557
Size: 3674
NspI r_CATG'y
Cuts at: 727 1227 2134 727
Size: 500 907 2267
Fragments arranged by size:
2267 907 500
PciI A'CATG_T
Cuts at: 2130 2130
Size: 3674
PflMI CCAn_nnn'nTGG
Cuts at: 303 303
Size: 3674
PmeI GTTT'AAAC
Cuts at: 673 673
Size: 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3642
PspOMI G'GGCC_C
Cuts at: 251 340 251
Size: 89 3585
PspXI vC'TCGA_Gb
Cuts at: 262 262
Size: 3674
PstI C_TGCA'G
Cuts at: 161 721 1406 161
Size: 560 685 2429
Fragments arranged by size:
2429 685 560
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3674
PvuII CAG'CTG
Cuts at: 1099 1459 1099
Size: 360 3314
RsrII CG'GwC_CG
Cuts at: 1869 1869
Size: 3674
SacI G_AGCT'C
Cuts at: 688 688
Size: 3674
SalI G'TCGA_C
Cuts at: 711 711
Size: 3674
SapI GCTCTTCn'nnn_
Cuts at: 590 1697 1907 590
Size: 1107 210 2357
Fragments arranged by size:
2357 1107 210
SbfI CC_TGCA'GG
Cuts at: 721 721
Size: 3674
ScaI AGT'ACT
Cuts at: 3191 3191
Size: 3674
SfcI C'TryA_G
Cuts at: 33 157 471 717 1402 2395 2586 33
Size: 124 314 246 685 993 191 1121
Fragments arranged by size:
1121 993 685 314 246 191 124
SfoI GGC'GCC
Cuts at: 311 1106 1354 311
Size: 795 248 2631
Fragments arranged by size:
2631 795 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
SmaI CCC'GGG
Cuts at: 696 696
Size: 3674
SmlI C'TyrA_G
Cuts at: 262 829 1744 2236 2498 262
Size: 567 915 492 262 1438
Fragments arranged by size:
1438 915 567 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3674
SphI G_CATG'C
Cuts at: 727 1227 727
Size: 500 3174
StyI C'CwwG_G
Cuts at: 808 1162 808
Size: 354 3320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 646 1257 1390 1849 2058 646
Size: 611 133 459 209 2262
Fragments arranged by size:
2262 611 459 209 133
TatI w'GTAC_w
Cuts at: 3189 3189
Size: 3674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 3642
Tth111I GACn'n_nGTC
Cuts at: 1471 1471
Size: 3674
XbaI T'CTAG_A
Cuts at: 705 705
Size: 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1169 114
Size: 1055 2619
XhoI C'TCGA_G
Cuts at: 262 262
Size: 3674
XmaI C'CCGG_G
Cuts at: 694 694
Size: 3674
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI