Restriction map for
pF1KB0956
(Circular) (Six-base) MAPSORT of: pF1KB0956.seq Check: 7678 from: 1 to: 4367
pF1KB0956 4367 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1021 2192 2624 3371 3639 544
Size: 477 1171 432 747 268 1272
Fragments arranged by size:
1272 1171 747 477 432 268
AflIII A'CryG_T
Cuts at: 128 1719 2823 3800 128
Size: 1591 1104 977 695
Fragments arranged by size:
1591 1104 977 695
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AleI CACnn'nnGTG
Cuts at: 1058 1058
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1130 1130
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 366 390 490 510 994 1261 1672 3239 366
Size: 24 100 20 484 267 411 1567 1494
Fragments arranged by size:
1567 1494 484 411 267 100 24 20
ApaLI G'TGCA_C
Cuts at: 408 3137 408
Size: 2729 1638
ApoI r'AATT_y
Cuts at: 742 1145 1371 3720 3976 742
Size: 403 226 2349 256 1133
Fragments arranged by size:
2349 1133 403 256 226
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 1383 1797 2045 2080 1383
Size: 414 248 35 3670
Fragments arranged by size:
3670 414 248 35
BanII G_rGCy'C
Cuts at: 1381 2411 1381
Size: 1030 3337
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 336 412 517 1067 1992 2085 3141 336
Size: 76 105 550 925 93 1056 1562
Fragments arranged by size:
1562 1056 925 550 105 93 76
BmgBI CAC'GTC
Cuts at: 129 129
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 675 1579 1660 1995 4139 675
Size: 904 81 335 2144 903
Fragments arranged by size:
2144 904 903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 940 1016 1141 806
Size: 134 76 125 4032
Fragments arranged by size:
4032 134 125 76
Bpu10I CC'TnA_GC
Cuts at: 889 889
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 2914 3212 1543
Size: 879 492 298 2698
Fragments arranged by size:
2698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 174 238 842 1069 4071 174
Size: 64 604 227 3002 470
Fragments arranged by size:
3002 604 470 227 64
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 953 1798 2046 2748 4244 953
Size: 845 248 702 1496 1076
Fragments arranged by size:
1496 1076 845 702 248
BsaWI w'CCGG_w
Cuts at: 233 833 1556 2077 2755 3029 3176 3587 233
Size: 600 723 521 678 274 147 411 1013
Fragments arranged by size:
1013 723 678 600 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 118
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 588 724 1315 1318 1321 1324 364
Size: 224 136 591 3 3 3 3407
Fragments arranged by size:
3407 591 224 136 3 3 3
BseYI C'CCAG_C
Cuts at: 1338 1792 2550 3127 3623 1338
Size: 454 758 577 496 2082
Fragments arranged by size:
2082 758 577 496 454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 488 260
Size: 228 4139
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 412 1381 2159 2349 3141 412
Size: 969 778 190 792 1638
Fragments arranged by size:
1638 969 792 778 190
BsmI GAATG_Cn'
Cuts at: 297 360 297
Size: 63 4304
Bsp1286I G_dGCh'C
Cuts at: 336 412 517 1067 1381 1992 2085 2159 2349
Size: 76 105 550 314 611 93 74 190
Cuts at: 2349 2411 3141 336
Size: 62 730 1562
Fragments arranged by size:
1562 730 611 550 314 190 105 93
76 74 62
BspEI T'CCGG_A
Cuts at: 233 3587 233
Size: 3354 1013
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1417 1631 1933 2314 1417
Size: 214 302 381 3470
Fragments arranged by size:
3470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 444 2279 444
Size: 1835 2532
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BssHII G'CGCG_C
Cuts at: 161 161
Size: 4367
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 1251 1251
Size: 4367
BstYI r'GATC_y
Cuts at: 1 1018 1205 1392 1859 1882 2217 2463 3475
Size: 1017 187 187 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1017 1012 467 449 346 335 246 187
187 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 328 932 1210 328
Size: 604 278 3485
Fragments arranged by size:
3485 604 278
BtgI C'CryG_G
Cuts at: 107 173 107
Size: 66 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 321 471 1112 1610 321
Size: 150 641 498 3078
Fragments arranged by size:
3078 641 498 150
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 629 864 1952 2126 2517 2544 3889 629
Size: 235 1088 174 391 27 1345 1107
Fragments arranged by size:
1345 1107 1088 391 235 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 701 781 1280 1286 1292 2390 2600 701
Size: 80 499 6 6 1098 210 2468
Fragments arranged by size:
2468 1098 499 210 80 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 806 940 1016 1021 1141 2192 2624 3371
Size: 262 134 76 5 120 1051 432 747
Cuts at: 3371 3639 544
Size: 268 1272
Fragments arranged by size:
1272 1051 747 432 268 262 134 120
76 5
EcoNI CCTnn'n_nnAGG
Cuts at: 793 1255 793
Size: 462 3905
EcoO109I rG'GnC_Cy
Cuts at: 507 520 786 1506 507
Size: 13 266 720 3368
Fragments arranged by size:
3368 720 266 13
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 418 2148 418
Size: 1730 2637
HaeII r_GCGC'y
Cuts at: 200 1801 2049 3071 4050 200
Size: 1601 248 1022 979 517
Fragments arranged by size:
1601 1022 979 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 1010 1042 2209 2239 2241 2271 118
Size: 32 860 32 1167 30 2 30 2214
Fragments arranged by size:
2214 1167 860 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
HindIII A'AGCT_T
Cuts at: 556 556
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 643 3038 3222 643
Size: 2395 184 1788
Fragments arranged by size:
2395 1788 184
MscI TGG'CCA
Cuts at: 631 866 2128 631
Size: 235 1262 2870
Fragments arranged by size:
2870 1262 235
MslI CAynn'nnrTG
Cuts at: 613 908 1058 2356 2483 613
Size: 295 150 1298 127 2497
Fragments arranged by size:
2497 1298 295 150 127
MspA1I CmG'CkG
Cuts at: 1176 1478 1792 2152 3165 3410 1176
Size: 302 314 360 1013 245 2133
Fragments arranged by size:
2133 1013 360 314 302 245
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 107 107
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1111 1420 1920 2827 1111
Size: 309 500 907 2651
Fragments arranged by size:
2651 907 500 309
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 507 507
Size: 4367
PshAI GACnn'nnGTC
Cuts at: 1169 1169
Size: 4367
PstI C_TGCA'G
Cuts at: 466 1414 2099 466
Size: 948 685 2734
Fragments arranged by size:
2734 948 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SexAI A'CCwGG_T
Cuts at: 503 503
Size: 4367
SfcI C'TryA_G
Cuts at: 33 462 1410 2095 3088 3279 33
Size: 429 948 685 993 191 1121
Fragments arranged by size:
1121 993 948 685 429 191
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 1522 2437 2929 3191 1522
Size: 915 492 262 2698
Fragments arranged by size:
2698 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 107 200 867 1501 1855 107
Size: 93 667 634 354 2619
Fragments arranged by size:
2619 667 634 354 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 413 1950 2083 2542 2751 413
Size: 1537 133 459 209 2029
Fragments arranged by size:
2029 1537 459 209 133
TatI w'GTAC_w
Cuts at: 458 3882 458
Size: 3424 943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 3884 3916 215
Size: 32 3637 32 666
Fragments arranged by size:
3637 666 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI
BsmBI BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI