Restriction map for
pF1KB0959
(Circular) (Six-base) MAPSORT of: pF1KB0959.seq Check: 1309 from: 1 to: 3683
pF1KB0959 3683 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 721 721
Size: 3683
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 348 408 540 573 666 988 2555 348
Size: 60 132 33 93 322 1567 1476
Fragments arranged by size:
1567 1476 322 132 93 60 33
ApaLI G'TGCA_C
Cuts at: 454 2453 454
Size: 1999 1684
ApoI r'AATT_y
Cuts at: 687 3036 3292 687
Size: 2349 256 1078
Fragments arranged by size:
2349 1078 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 703 703
Size: 3683
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 127 333 699 1113 1361 1396 127
Size: 206 366 414 248 35 2414
Fragments arranged by size:
2414 414 366 248 206 35
BanII G_rGCy'C
Cuts at: 697 1727 697
Size: 1030 2653
BbeI G_GCGC'C
Cuts at: 337 1117 1365 337
Size: 780 248 2655
Fragments arranged by size:
2655 780 248
BciVI GTATCCnnnnn_n'
Cuts at: 1581 2342 3683 1581
Size: 761 1341 1581
Fragments arranged by size:
1581 1341 761
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BglI GCCn_nnn'nGGC
Cuts at: 392 531 392
Size: 139 3544
BglII A'GATC_T
Cuts at: 1198 3235 1198
Size: 2037 1646
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 130 458 595 1308 1401 2457 130
Size: 328 137 713 93 1056 1356
Fragments arranged by size:
1356 1056 713 328 137 93
BmgBI CAC'GTC
Cuts at: 167 167
Size: 3683
BmrI ACTGGGnnnn_n'
Cuts at: 895 976 1311 3455 895
Size: 81 335 2144 1123
Fragments arranged by size:
2144 1123 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 3683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1738 2230 2528 859
Size: 879 492 298 2014
Fragments arranged by size:
2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3387 3387
Size: 3683
BsaAI yAC'GTr
Cuts at: 555 1036 555
Size: 481 3202
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 334 622 1114 1362 2064 3560 334
Size: 288 492 248 702 1496 457
Fragments arranged by size:
1496 702 492 457 288 248
BsaWI w'CCGG_w
Cuts at: 443 872 1393 2071 2345 2492 2903 443
Size: 429 521 678 274 147 411 1223
Fragments arranged by size:
1223 678 521 429 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 268
Size: 30 3653
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 198 198
Size: 3683
BseYI C'CCAG_C
Cuts at: 516 588 1108 1866 2443 2939 516
Size: 72 520 758 577 496 1260
Fragments arranged by size:
1260 758 577 520 496 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 3683
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 451 458 697 1475 1665 2457 451
Size: 7 239 778 190 792 1677
Fragments arranged by size:
1677 792 778 239 190 7
BsmBI CGTCTCn'nnnn_
Cuts at: 613 613
Size: 3683
Bsp1286I G_dGCh'C
Cuts at: 130 451 458 595 697 1308 1401 1475 1665
Size: 321 7 137 102 611 93 74 190
Cuts at: 1665 1727 2457 130
Size: 62 730 1356
Fragments arranged by size:
1356 730 611 321 190 137 102 93
74 62 7
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 416 733 947 1249 1630 416
Size: 317 214 302 381 2469
Fragments arranged by size:
2469 381 317 302 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 336 547 1681 1862 2071 336
Size: 211 1134 181 209 1948
Fragments arranged by size:
1948 1134 211 209 181
BssHII G'CGCG_C
Cuts at: 436 436
Size: 3683
BssSI C'ACGA_G
Cuts at: 146 1954 2312 146
Size: 1808 358 1517
Fragments arranged by size:
1808 1517 358
BstAPI GCAn_nnn'nTGC
Cuts at: 540 1060 540
Size: 520 3163
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstEII G'GTnAC_C
Cuts at: 462 462
Size: 3683
BstYI r'GATC_y
Cuts at: 1 708 1175 1198 1533 1779 2791 2877 2889
Size: 707 467 23 335 246 1012 86 12
Cuts at: 2889 3235 1
Size: 346 449
Fragments arranged by size:
1012 707 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 370 370
Size: 3683
BtgI C'CryG_G
Cuts at: 107 107
Size: 3683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1633 1814 1977 1633
Size: 181 163 3339
Fragments arranged by size:
3339 181 163
BtsI GCAGTG_nn'
Cuts at: 926 926
Size: 3683
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DraIII CAC_nnn'GTG
Cuts at: 462 462
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 549 1268 1442 1833 1860 3205 549
Size: 719 174 391 27 1345 1027
Fragments arranged by size:
1345 1027 719 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 196 1706 1916 196
Size: 1510 210 1963
Fragments arranged by size:
1963 1510 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1891 2201 2347 3493 1891
Size: 310 146 1146 2081
Fragments arranged by size:
2081 1146 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1508 1940 2687 2955 595
Size: 913 432 747 268 1323
Fragments arranged by size:
1323 913 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 134 155 316 345 375 822 134
Size: 21 161 29 30 447 2995
Fragments arranged by size:
2995 447 161 30 29 21
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1702 1702
Size: 3683
FspI TGC'GCA
Cuts at: 412 559 1464 412
Size: 147 905 2631
Fragments arranged by size:
2631 905 147
HaeII r_GCGC'y
Cuts at: 337 1117 1365 2387 3366 337
Size: 780 248 1022 979 654
Fragments arranged by size:
1022 979 780 654 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1525 1555 1557 1587 1525
Size: 30 2 30 3621
Fragments arranged by size:
3621 30 30 2
HincII GTy'rAC
Cuts at: 722 722
Size: 3683
KasI G'GCGC_C
Cuts at: 333 1113 1361 333
Size: 780 248 2655
Fragments arranged by size:
2655 780 248
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2538 2354
Size: 184 3499
MscI TGG'CCA
Cuts at: 1444 1444
Size: 3683
MslI CAynn'nnrTG
Cuts at: 1672 1799 1672
Size: 127 3556
MspA1I CmG'CkG
Cuts at: 416 540 666 794 1108 1468 2481 2726 416
Size: 124 126 128 314 360 1013 245 1373
Fragments arranged by size:
1373 1013 360 314 245 128 126 124
NaeI GCC'GGC
Cuts at: 549 1864 549
Size: 1315 2368
NarI GG'CG_CC
Cuts at: 334 1114 1362 334
Size: 780 248 2655
Fragments arranged by size:
2655 780 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3683
NgoMIV G'CCGG_C
Cuts at: 547 1862 547
Size: 1315 2368
NspI r_CATG'y
Cuts at: 386 736 1236 2143 386
Size: 350 500 907 1926
Fragments arranged by size:
1926 907 500 350
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PmlI CAC'GTG
Cuts at: 555 555
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PpuMI rG'GwC_Cy
Cuts at: 134 375 134
Size: 241 3442
PstI C_TGCA'G
Cuts at: 569 611 730 1415 569
Size: 42 119 685 2837
Fragments arranged by size:
2837 685 119 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 666 1108 1468 666
Size: 442 360 2881
Fragments arranged by size:
2881 442 360
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SanDI GG'GwC_CC
Cuts at: 375 375
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 569 730 569
Size: 161 3522
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 565 607 726 1411 2404 2595 33
Size: 532 42 119 685 993 191 1121
Fragments arranged by size:
1121 993 685 532 191 119 42
SfoI GGC'GCC
Cuts at: 335 1115 1363 335
Size: 780 248 2655
Fragments arranged by size:
2655 780 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SgrAI Cr'CCGG_yG
Cuts at: 336 336
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 838 1753 2245 2507 838
Size: 915 492 262 2014
Fragments arranged by size:
2014 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StuI AGG'CCT
Cuts at: 472 472
Size: 3683
StyI C'CwwG_G
Cuts at: 107 817 1171 107
Size: 710 354 2619
Fragments arranged by size:
2619 710 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1399 1858 2067 1266
Size: 133 459 209 2882
Fragments arranged by size:
2882 459 209 133
TatI w'GTAC_w
Cuts at: 3198 3198
Size: 3683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 151 183 3200 3232 151
Size: 32 3017 32 602
Fragments arranged by size:
3017 602 32 32
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 714 714
Size: 3683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1178 1178
Size: 3683
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI Bpu10I BsiWI BsmI BsrGI BstXI BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI