Restriction map for
pF1KB0961
(Circular) (Six-base) MAPSORT of: pF1KB0961.seq Check: 8573 from: 1 to: 3692
pF1KB0961 3692 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1517 1949 2696 2964 1517
Size: 432 747 268 2245
Fragments arranged by size:
2245 747 432 268
AfeI AGC'GCT
Cuts at: 460 460
Size: 3692
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 997 2564 997
Size: 1567 2125
ApaI G_GGCC'C
Cuts at: 311 638 311
Size: 327 3365
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 430 696 3045 3301 430
Size: 266 2349 256 821
Fragments arranged by size:
2349 821 266 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 712 712
Size: 3692
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 340 708 1122 1370 1405 340
Size: 368 414 248 35 2627
Fragments arranged by size:
2627 414 368 248 35
BanII G_rGCy'C
Cuts at: 311 638 706 1736 311
Size: 327 68 1030 2267
Fragments arranged by size:
2267 1030 327 68
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbsI GAAGACnn'nnnn_
Cuts at: 157 157
Size: 3692
BbvCI CC'TCA_GC
Cuts at: 346 346
Size: 3692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3658
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BfrBI ATG'CAT
Cuts at: 424 424
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 311 638 1317 1410 2466 311
Size: 327 679 93 1056 1537
Fragments arranged by size:
1537 1056 679 327 93
BmrI ACTGGGnnnn_n'
Cuts at: 126 904 985 1320 3464 126
Size: 778 81 335 2144 354
Fragments arranged by size:
2144 778 354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 3692
Bpu10I CC'TnA_GC
Cuts at: 346 346
Size: 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 868 1747 2239 2537 586
Size: 282 879 492 298 1741
Fragments arranged by size:
1741 879 492 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 137 283 137
Size: 146 3546
BseYI C'CCAG_C
Cuts at: 369 511 664 1117 1875 2452 2948 369
Size: 142 153 453 758 577 496 1113
Fragments arranged by size:
1113 758 577 496 453 153 142
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 706 1484 1674 2466 706
Size: 778 190 792 1932
Fragments arranged by size:
1932 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 311 638 706 1317 1410 1484 1674 1736 2466
Size: 327 68 611 93 74 190 62 730
Cuts at: 2466 311
Size: 1537
Fragments arranged by size:
1537 730 611 327 190 93 74 68
62
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 742 956 1258 1639 742
Size: 214 302 381 2795
Fragments arranged by size:
2795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 1690 1871 2080 1690
Size: 181 209 3302
Fragments arranged by size:
3302 209 181
BssSI C'ACGA_G
Cuts at: 382 1963 2321 382
Size: 1581 358 1753
Fragments arranged by size:
1753 1581 358
BstAPI GCAn_nnn'nTGC
Cuts at: 224 287 487 1069 224
Size: 63 200 582 2847
Fragments arranged by size:
2847 582 200 63
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstXI CCAn_nnnn'nTGG
Cuts at: 445 445
Size: 3692
BstYI r'GATC_y
Cuts at: 1 717 1184 1207 1542 1788 2800 2886 2898
Size: 716 467 23 335 246 1012 86 12
Cuts at: 2898 3244 1
Size: 346 449
Fragments arranged by size:
1012 716 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 935 935
Size: 3692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 117 152 117
Size: 35 3657
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 254 396 1277 1451 1842 1869 3214 254
Size: 142 881 174 391 27 1345 732
Fragments arranged by size:
1345 881 732 391 174 142 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 164 1715 1925 164
Size: 1551 210 1931
Fragments arranged by size:
1931 1551 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1517 1949 2696 2964 1517
Size: 432 747 268 2245
Fragments arranged by size:
2245 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 668 831 668
Size: 163 3529
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 1711 552
Size: 1159 2533
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 462 1126 1374 2396 3375 462
Size: 664 248 1022 979 779
Fragments arranged by size:
1022 979 779 664 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1534 1564 1566 1596 1534
Size: 30 2 30 3630
Fragments arranged by size:
3630 30 30 2
HincII GTy'rAC
Cuts at: 572 731 572
Size: 159 3533
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2547 2363
Size: 184 3508
MscI TGG'CCA
Cuts at: 398 1453 398
Size: 1055 2637
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 803 1117 1477 2490 2735 803
Size: 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NsiI A_TGCA'T
Cuts at: 426 426
Size: 3692
NspI r_CATG'y
Cuts at: 745 1245 2152 745
Size: 500 907 2285
Fragments arranged by size:
2285 907 500
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PspOMI G'GGCC_C
Cuts at: 307 634 307
Size: 327 3365
PstI C_TGCA'G
Cuts at: 739 1424 739
Size: 685 3007
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 735 1420 2413 2604 33
Size: 702 685 993 191 1121
Fragments arranged by size:
1121 993 702 685 191
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 565 847 1762 2254 2516 565
Size: 282 915 492 262 1741
Fragments arranged by size:
1741 915 492 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StuI AGG'CCT
Cuts at: 532 563 532
Size: 31 3661
StyI C'CwwG_G
Cuts at: 638 671 826 1180 638
Size: 33 155 354 3150
Fragments arranged by size:
3150 354 155 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1408 1867 2076 1275
Size: 133 459 209 2891
Fragments arranged by size:
2891 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 445 1187 445
Size: 742 2950
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
XmnI GAAnn'nnTTC
Cuts at: 173 173
Size: 3692
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BglI BmgBI BmtI BpmI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI