Restriction map for
pF1KB0967
(Circular) (Six-base) MAPSORT of: pF1KB0967.seq Check: 8441 from: 1 to: 3731
pF1KB0967 3731 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1988 2735 3003 1556
Size: 432 747 268 2284
Fragments arranged by size:
2284 747 432 268
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 671 671
Size: 3731
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 2501 2501
Size: 3731
ApoI r'AATT_y
Cuts at: 735 3084 3340 735
Size: 2349 256 1126
Fragments arranged by size:
2349 1126 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 133 168 386 751 133
Size: 35 218 365 3113
Fragments arranged by size:
3113 365 218 35
AvrII C'CTAG_G
Cuts at: 315 315
Size: 3731
BamHI G'GATC_C
Cuts at: 756 756
Size: 3731
BanI G'GyrC_C
Cuts at: 269 319 344 747 1161 1409 1444 269
Size: 50 25 403 414 248 35 2556
Fragments arranged by size:
2556 414 403 248 50 35 25
BanII G_rGCy'C
Cuts at: 134 169 199 745 1775 134
Size: 35 30 546 1030 2090
Fragments arranged by size:
2090 1030 546 35 30
BbeI G_GCGC'C
Cuts at: 273 348 1165 1413 273
Size: 75 817 248 2591
Fragments arranged by size:
2591 817 248 75
BbvCI CC'TCA_GC
Cuts at: 277 548 626 277
Size: 271 78 3382
Fragments arranged by size:
3382 271 78
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglII A'GATC_T
Cuts at: 1246 3283 1246
Size: 2037 1694
BlpI GC'TnA_GC
Cuts at: 392 843 392
Size: 451 3280
Bme1580I G_kGCm'C
Cuts at: 1356 1449 2505 1356
Size: 93 1056 2582
Fragments arranged by size:
2582 1056 93
BmgBI CAC'GTC
Cuts at: 459 459
Size: 3731
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 183
Size: 3731
Bpu10I CC'TnA_GC
Cuts at: 277 548 626 277
Size: 271 78 3382
Fragments arranged by size:
3382 271 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 907 1786 2278 2576 439
Size: 468 879 492 298 1594
Fragments arranged by size:
1594 879 492 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 1084 1084
Size: 3731
BsaBI GATnn'nnATC
Cuts at: 6 1245 3282 6
Size: 1239 2037 455
Fragments arranged by size:
2037 1239 455
BsaHI Gr'CG_yC
Cuts at: 270 345 517 1162 1410 2112 3608 270
Size: 75 172 645 248 702 1496 393
Fragments arranged by size:
1496 702 645 393 248 172 75
BsaWI w'CCGG_w
Cuts at: 920 1441 2119 2393 2540 2951 920
Size: 521 678 274 147 411 1700
Fragments arranged by size:
1700 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 396 694 1156 1914 2491 2987 396
Size: 298 462 758 577 496 1140
Fragments arranged by size:
1140 758 577 496 462 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 631 105
Size: 526 3205
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 745 1523 1713 2505 745
Size: 778 190 792 1971
Fragments arranged by size:
1971 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 134 169 199 745 1356 1449 1523 1713 1775
Size: 35 30 546 611 93 74 190 62
Cuts at: 1775 2505 134
Size: 730 1360
Fragments arranged by size:
1360 730 611 546 190 93 74 62
35 30
BspEI T'CCGG_A
Cuts at: 2951 2951
Size: 3731
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 522 653 781 995 1297 1678 522
Size: 131 128 214 302 381 2575
Fragments arranged by size:
2575 381 302 214 131 128
BsrBI CCG'CTC
Cuts at: 50 2023 2077 3561 3726 50
Size: 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 511 1643 511
Size: 1132 2599
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BssSI C'ACGA_G
Cuts at: 2002 2360 2002
Size: 358 3373
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1108
Size: 3731
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstXI CCAn_nnnn'nTGG
Cuts at: 374 374
Size: 3731
BstYI r'GATC_y
Cuts at: 1 756 1223 1246 1581 1827 2839 2925 2937
Size: 755 467 23 335 246 1012 86 12
Cuts at: 2937 3283 1
Size: 346 449
Fragments arranged by size:
1012 755 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 126 126
Size: 3731
BtgI C'CryG_G
Cuts at: 107 107
Size: 3731
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1681 1862 2025 1681
Size: 181 163 3387
Fragments arranged by size:
3387 181 163
BtsI GCAGTG_nn'
Cuts at: 627 974 627
Size: 347 3384
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 245 1316 1490 1881 1908 3253 245
Size: 1071 174 391 27 1345 723
Fragments arranged by size:
1345 1071 723 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 2249 2395 3541 1939
Size: 310 146 1146 2129
Fragments arranged by size:
2129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 1556 1988 2735 3003 183
Size: 1373 432 747 268 911
Fragments arranged by size:
1373 911 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 491 491
Size: 3731
EcoO109I rG'GnC_Cy
Cuts at: 158 534 630 705 870 158
Size: 376 96 75 165 3019
Fragments arranged by size:
3019 376 165 96 75
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 1750
Size: 3731
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 273 348 686 1165 1413 2435 3414 273
Size: 75 338 479 248 1022 979 590
Fragments arranged by size:
1022 979 590 479 338 248 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 1573 1603 1605 1635 385
Size: 32 1156 30 2 30 2481
Fragments arranged by size:
2481 1156 32 30 30 2
HincII GTy'rAC
Cuts at: 770 770
Size: 3731
KasI G'GCGC_C
Cuts at: 269 344 1161 1409 269
Size: 75 817 248 2591
Fragments arranged by size:
2591 817 248 75
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 247 1492 247
Size: 1245 2486
MslI CAynn'nnrTG
Cuts at: 1720 1847 1720
Size: 127 3604
MspA1I CmG'CkG
Cuts at: 694 842 1156 1516 2529 2774 694
Size: 148 314 360 1013 245 1651
Fragments arranged by size:
1651 1013 360 314 245 148
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 270 345 1162 1410 270
Size: 75 817 248 2591
Fragments arranged by size:
2591 817 248 75
NcoI C'CATG_G
Cuts at: 107 107
Size: 3731
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NspI r_CATG'y
Cuts at: 784 1284 2191 784
Size: 500 907 2324
Fragments arranged by size:
2324 907 500
PasI CC'CwG_GG
Cuts at: 487 487
Size: 3731
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PpuMI rG'GwC_Cy
Cuts at: 534 534
Size: 3731
PstI C_TGCA'G
Cuts at: 383 479 778 1463 383
Size: 96 299 685 2651
Fragments arranged by size:
2651 685 299 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 694 1156 1516 694
Size: 462 360 2909
Fragments arranged by size:
2909 462 360
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 479 778 479
Size: 299 3432
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SexAI A'CCwGG_T
Cuts at: 581 581
Size: 3731
SfcI C'TryA_G
Cuts at: 33 379 475 774 1459 2452 2643 33
Size: 346 96 299 685 993 191 1121
Fragments arranged by size:
1121 993 685 346 299 191 96
SfoI GGC'GCC
Cuts at: 271 346 1163 1411 271
Size: 75 817 248 2591
Fragments arranged by size:
2591 817 248 75
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 418 886 1801 2293 2555 418
Size: 468 915 492 262 1594
Fragments arranged by size:
1594 915 492 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 107 161 315 865 1219 107
Size: 54 154 550 354 2619
Fragments arranged by size:
2619 550 354 154 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1447 1906 2115 1314
Size: 133 459 209 2930
Fragments arranged by size:
2930 459 209 133
TatI w'GTAC_w
Cuts at: 3246 3246
Size: 3731
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 457 583 1528 457
Size: 126 945 2660
Fragments arranged by size:
2660 945 126
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 463 1226 463
Size: 763 2968
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BfrBI BglI BmtI BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI