Restriction map for
pF1KB0971
(Circular) (Six-base) MAPSORT of: pF1KB0971.seq Check: 9981 from: 1 to: 3740
pF1KB0971 3740 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 127 127
Size: 3740
AccI GT'mk_AC
Cuts at: 778 778
Size: 3740
Acc65I G'GTAC_C
Cuts at: 756 756
Size: 3740
AclI AA'CG_TT
Cuts at: 3580 3580
Size: 3740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1565 1997 2744 3012 655
Size: 910 432 747 268 1383
Fragments arranged by size:
1383 910 747 432 268
AflIII A'CryG_T
Cuts at: 1092 2196 3173 1092
Size: 1104 977 1659
Fragments arranged by size:
1659 1104 977
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 159 159
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 219 1045 2612 219
Size: 826 1567 1347
Fragments arranged by size:
1567 1347 826
ApaI G_GGCC'C
Cuts at: 652 652
Size: 3740
ApaLI G'TGCA_C
Cuts at: 328 656 2510 328
Size: 328 1854 1558
Fragments arranged by size:
1854 1558 328
ApoI r'AATT_y
Cuts at: 744 3093 3349 744
Size: 2349 256 1135
Fragments arranged by size:
2349 1135 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 169 194 760 169
Size: 25 566 3149
Fragments arranged by size:
3149 566 25
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 165 177 756 1170 1418 1453 165
Size: 12 579 414 248 35 2452
Fragments arranged by size:
2452 579 414 248 35 12
BanII G_rGCy'C
Cuts at: 115 652 754 1784 115
Size: 537 102 1030 2071
Fragments arranged by size:
2071 1030 537 102
BbeI G_GCGC'C
Cuts at: 169 181 1174 1422 169
Size: 12 993 248 2487
Fragments arranged by size:
2487 993 248 12
BbvCI CC'TCA_GC
Cuts at: 212 212
Size: 3740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 246 280 246
Size: 34 3706
BciVI GTATCCnnnnn_n'
Cuts at: 1638 2399 3740 1638
Size: 761 1341 1638
Fragments arranged by size:
1638 1341 761
BclI T'GATC_A
Cuts at: 1260 1260
Size: 3740
BglI GCCn_nnn'nGGC
Cuts at: 154 199 154
Size: 45 3695
BglII A'GATC_T
Cuts at: 1255 3292 1255
Size: 2037 1703
BlpI GC'TnA_GC
Cuts at: 852 852
Size: 3740
Bme1580I G_kGCm'C
Cuts at: 332 652 660 1365 1458 2514 332
Size: 320 8 705 93 1056 1558
Fragments arranged by size:
1558 1056 705 320 93 8
BmrI ACTGGGnnnn_n'
Cuts at: 307 952 1033 1368 3512 307
Size: 645 81 335 2144 535
Fragments arranged by size:
2144 645 535 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 3740
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 3740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1795 2287 2585 916
Size: 879 492 298 2071
Fragments arranged by size:
2071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3444 3444
Size: 3740
BsaAI yAC'GTr
Cuts at: 1093 1093
Size: 3740
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 166 178 1171 1419 2121 3617 166
Size: 12 993 248 702 1496 289
Fragments arranged by size:
1496 993 702 289 248 12
BsaWI w'CCGG_w
Cuts at: 359 436 929 1450 2128 2402 2549 2960 359
Size: 77 493 521 678 274 147 411 1139
Fragments arranged by size:
1139 678 521 493 411 274 147 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 187
Size: 3740
BseYI C'CCAG_C
Cuts at: 575 641 1165 1923 2500 2996 575
Size: 66 524 758 577 496 1319
Fragments arranged by size:
1319 758 577 524 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 328 205
Size: 123 3617
BsiEI CG_ry'CG
Cuts at: 104 1282 1328 2536 104
Size: 1178 46 1208 1308
Fragments arranged by size:
1308 1208 1178 46
BsiHKAI G_wGCw'C
Cuts at: 332 660 754 1532 1722 2514 332
Size: 328 94 778 190 792 1558
Fragments arranged by size:
1558 792 778 328 190 94
BsmI GAATG_Cn'
Cuts at: 565 565
Size: 3740
Bsp1286I G_dGCh'C
Cuts at: 115 332 652 660 754 1365 1458 1532 1722
Size: 217 320 8 94 611 93 74 190
Cuts at: 1722 1784 2514 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 320 217 190 94 93
74 62 8
BspEI T'CCGG_A
Cuts at: 359 2960 359
Size: 2601 1139
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 127 790 1004 1306 1687 127
Size: 663 214 302 381 2180
Fragments arranged by size:
2180 663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2032 2086 3570 3735 50
Size: 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 1738 1919 2128 1738
Size: 181 209 3350
Fragments arranged by size:
3350 209 181
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 1117 1117
Size: 3740
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstXI CCAn_nnnn'nTGG
Cuts at: 417 417
Size: 3740
BstYI r'GATC_y
Cuts at: 1 765 1232 1255 1590 1836 2848 2934 2946
Size: 764 467 23 335 246 1012 86 12
Cuts at: 2946 3292 1
Size: 346 449
Fragments arranged by size:
1012 764 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3740
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1690 1871 2034 1690
Size: 181 163 3396
Fragments arranged by size:
3396 181 163
BtsI GCAGTG_nn'
Cuts at: 389 983 389
Size: 594 3146
DraI TTT'AAA
Cuts at: 739 739
Size: 3740
DrdI GACnn_nn'nnGTC
Cuts at: 1446 2304 1446
Size: 858 2882
EaeI y'GGCC_r
Cuts at: 475 1325 1499 1890 1917 3262 475
Size: 850 174 391 27 1345 953
Fragments arranged by size:
1345 953 850 391 174 27
EagI C'GGCC_G
Cuts at: 1325 1325
Size: 3740
EarI CTCTTCn'nnn_
Cuts at: 155 1763 1973 155
Size: 1608 210 1922
Fragments arranged by size:
1922 1608 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 217 1948 2258 2404 3550 217
Size: 1731 310 146 1146 407
Fragments arranged by size:
1731 1146 407 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1565 1997 2744 3012 655
Size: 910 432 747 268 1383
Fragments arranged by size:
1383 910 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 648 649 879 648
Size: 1 230 3509
Fragments arranged by size:
3509 230 1
EcoRI G'AATT_C
Cuts at: 744 744
Size: 3740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 609 1759 609
Size: 1150 2590
FspI TGC'GCA
Cuts at: 256 1521 256
Size: 1265 2475
FspAI rTGC'GCAy
Cuts at: 256 256
Size: 3740
HaeII r_GCGC'y
Cuts at: 169 181 1174 1422 2444 3423 169
Size: 12 993 248 1022 979 486
Fragments arranged by size:
1022 993 979 486 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1582 1612 1614 1644 1582
Size: 30 2 30 3678
Fragments arranged by size:
3678 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
KasI G'GCGC_C
Cuts at: 165 177 1170 1418 165
Size: 12 993 248 2487
Fragments arranged by size:
2487 993 248 12
KpnI G_GTAC'C
Cuts at: 760 760
Size: 3740
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 146 2411 2595 146
Size: 2265 184 1291
Fragments arranged by size:
2265 1291 184
MscI TGG'CCA
Cuts at: 1501 1501
Size: 3740
MslI CAynn'nnrTG
Cuts at: 1729 1856 1729
Size: 127 3613
MspA1I CmG'CkG
Cuts at: 216 633 851 1165 1525 2538 2783 216
Size: 417 218 314 360 1013 245 1173
Fragments arranged by size:
1173 1013 417 360 314 245 218
NaeI GCC'GGC
Cuts at: 1921 1921
Size: 3740
NarI GG'CG_CC
Cuts at: 166 178 1171 1419 166
Size: 12 993 248 2487
Fragments arranged by size:
2487 993 248 12
NcoI C'CATG_G
Cuts at: 107 107
Size: 3740
NdeI CA'TA_TG
Cuts at: 614 614
Size: 3740
NgoMIV G'CCGG_C
Cuts at: 1919 1919
Size: 3740
NspI r_CATG'y
Cuts at: 495 793 1293 2200 495
Size: 298 500 907 2035
Fragments arranged by size:
2035 907 500 298
PciI A'CATG_T
Cuts at: 2196 2196
Size: 3740
PfoI T'CCnGG_A
Cuts at: 719 719
Size: 3740
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PspOMI G'GGCC_C
Cuts at: 648 648
Size: 3740
PstI C_TGCA'G
Cuts at: 787 1472 787
Size: 685 3055
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 216 1165 1525 216
Size: 949 360 2431
Fragments arranged by size:
2431 949 360
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SapI GCTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 3257 3257
Size: 3740
SexAI A'CCwGG_T
Cuts at: 441 441
Size: 3740
SfcI C'TryA_G
Cuts at: 33 783 1468 2461 2652 33
Size: 750 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 191
SfoI GGC'GCC
Cuts at: 167 179 1172 1420 167
Size: 12 993 248 2487
Fragments arranged by size:
2487 993 248 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SmaI CCC'GGG
Cuts at: 196 762 196
Size: 566 3174
SmlI C'TyrA_G
Cuts at: 895 1810 2302 2564 895
Size: 915 492 262 2071
Fragments arranged by size:
2071 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 495 793 1293 495
Size: 298 500 2942
Fragments arranged by size:
2942 500 298
SrfI GCCC'GGGC
Cuts at: 196 196
Size: 3740
StyI C'CwwG_G
Cuts at: 107 149 410 554 874 1228 107
Size: 42 261 144 320 354 2619
Fragments arranged by size:
2619 354 320 261 144 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1323 1456 1915 2124 1323
Size: 133 459 209 2939
Fragments arranged by size:
2939 459 209 133
TatI w'GTAC_w
Cuts at: 3255 3255
Size: 3740
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 1537 1537
Size: 3740
XbaI T'CTAG_A
Cuts at: 540 771 540
Size: 231 3509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1235
Size: 3740
XmaI C'CCGG_G
Cuts at: 194 760 194
Size: 566 3174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BpmI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI