Restriction map for
pF1KB0972
(Circular) (Six-base) MAPSORT of: pF1KB0972.seq Check: 6966 from: 1 to: 4424
pF1KB0972 4424 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 694 962 1269 694
Size: 268 307 3849
Fragments arranged by size:
3849 307 268
AccI GT'mk_AC
Cuts at: 1462 1462
Size: 4424
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 2249 2681 3428 3696 1115
Size: 1134 432 747 268 1843
Fragments arranged by size:
1843 1134 747 432 268
AflIII A'CryG_T
Cuts at: 128 1776 2880 3857 128
Size: 1648 1104 977 695
Fragments arranged by size:
1648 1104 977 695
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AhdI GACnn_n'nnGTC
Cuts at: 1062 1290 1062
Size: 228 4196
AlwNI CAG_nnn'CTG
Cuts at: 443 915 1152 1341 1729 3296 443
Size: 472 237 189 388 1567 1571
Fragments arranged by size:
1571 1567 472 388 237 189
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 250 262 518 982 1428 3777 4033 250
Size: 12 256 464 446 2349 256 641
Fragments arranged by size:
2349 641 464 446 256 256 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 1444 1444
Size: 4424
BamHI G'GATC_C
Cuts at: 1314 1449 1314
Size: 135 4289
BanI G'GyrC_C
Cuts at: 1440 1854 2102 2137 1440
Size: 414 248 35 3727
Fragments arranged by size:
3727 414 248 35
BanII G_rGCy'C
Cuts at: 189 929 1438 2468 189
Size: 740 509 1030 2145
Fragments arranged by size:
2145 1030 740 509
BbeI G_GCGC'C
Cuts at: 1858 2106 1858
Size: 248 4176
BbsI GAAGACnn'nnnn_
Cuts at: 553 1227 553
Size: 674 3750
BbvCI CC'TCA_GC
Cuts at: 1162 1162
Size: 4424
BciVI GTATCCnnnnn_n'
Cuts at: 143 2322 3083 4424 143
Size: 2179 761 1341 143
Fragments arranged by size:
2179 1341 761 143
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BglI GCCn_nnn'nGGC
Cuts at: 112 157 112
Size: 45 4379
BglII A'GATC_T
Cuts at: 1409 1939 3976 1409
Size: 530 2037 1857
Fragments arranged by size:
2037 1857 530
BlpI GC'TnA_GC
Cuts at: 845 923 1536 845
Size: 78 613 3733
Fragments arranged by size:
3733 613 78
Bme1580I G_kGCm'C
Cuts at: 431 2049 2142 3198 431
Size: 1618 93 1056 1657
Fragments arranged by size:
1657 1618 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 696 744 1636 1717 2052 4196 696
Size: 48 892 81 335 2144 924
Fragments arranged by size:
2144 924 892 335 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 4424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 356 1244 165
Size: 191 888 3345
Fragments arranged by size:
3345 888 191
Bpu10I CC'TnA_GC
Cuts at: 188 1162 188
Size: 974 3450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1600 2479 2971 3269 1600
Size: 879 492 298 2755
Fragments arranged by size:
2755 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 769 1324 4128 769
Size: 555 2804 1065
Fragments arranged by size:
2804 1065 555
BsaAI yAC'GTr
Cuts at: 131 1777 131
Size: 1646 2778
BsaBI GATnn'nnATC
Cuts at: 6 1938 3975 6
Size: 1932 2037 455
Fragments arranged by size:
2037 1932 455
BsaHI Gr'CG_yC
Cuts at: 1855 2103 2805 4301 1855
Size: 248 702 1496 1978
Fragments arranged by size:
1978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1613 2134 2812 3086 3233 3644 1613
Size: 521 678 274 147 411 2393
Fragments arranged by size:
2393 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1217 1247 1217
Size: 30 4394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 871 901 904 1342 367
Size: 504 30 3 438 3449
Fragments arranged by size:
3449 504 438 30 3
BseYI C'CCAG_C
Cuts at: 115 282 432 1849 2607 3184 3680 115
Size: 167 150 1417 758 577 496 859
Fragments arranged by size:
1417 859 758 577 496 167 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 588 415
Size: 173 4251
BsiEI CG_ry'CG
Cuts at: 104 1966 2012 3220 104
Size: 1862 46 1208 1308
Fragments arranged by size:
1862 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 1046 1438 2216 2406 3198 203
Size: 843 392 778 190 792 1429
Fragments arranged by size:
1429 843 792 778 392 190
BsmI GAATG_Cn'
Cuts at: 501 1176 501
Size: 675 3749
Bsp1286I G_dGCh'C
Cuts at: 189 203 431 929 1046 1438 2049 2142 2216
Size: 14 228 498 117 392 611 93 74
Cuts at: 2216 2406 2468 3198 189
Size: 190 62 730 1415
Fragments arranged by size:
1415 730 611 498 392 228 190 117
93 74 62 14
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 1115 3600 1115
Size: 2485 1939
BspMI ACCTGCnnnn'nnnn_
Cuts at: 618 694 962 1269 1474 1688 1990 2371 618
Size: 76 268 307 205 214 302 381 2671
Fragments arranged by size:
2671 381 307 302 268 214 205 76
BsrBI CCG'CTC
Cuts at: 50 849 2716 2770 4254 4419 50
Size: 799 1867 54 1484 165 55
Fragments arranged by size:
1867 1484 799 165 55 54
BsrDI GCAATG_nn'
Cuts at: 329 354 2336 329
Size: 25 1982 2417
Fragments arranged by size:
2417 1982 25
BsrFI r'CCGG_y
Cuts at: 271 2422 2603 2812 271
Size: 2151 181 209 1883
Fragments arranged by size:
2151 1883 209 181
BsrGI T'GTAC_A
Cuts at: 125 125
Size: 4424
BssSI C'ACGA_G
Cuts at: 2695 3053 2695
Size: 358 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 1801 1801
Size: 4424
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 1037 1037
Size: 4424
BstYI r'GATC_y
Cuts at: 1 1314 1409 1449 1916 1939 2274 2520 3532
Size: 1313 95 40 467 23 335 246 1012
Cuts at: 3532 3618 3630 3976 1
Size: 86 12 346 449
Fragments arranged by size:
1313 1012 467 449 346 335 246 95
86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 884 884
Size: 4424
BtgI C'CryG_G
Cuts at: 107 799 107
Size: 692 3732
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 150 2374 2555 2718 150
Size: 2224 181 163 1856
Fragments arranged by size:
2224 1856 181 163
BtsI GCAGTG_nn'
Cuts at: 403 1024 1667 403
Size: 621 643 3160
Fragments arranged by size:
3160 643 621
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 2130 2988 2130
Size: 858 3566
EaeI y'GGCC_r
Cuts at: 228 2009 2183 2574 2601 3946 228
Size: 1781 174 391 27 1345 706
Fragments arranged by size:
1781 1345 706 391 174 27
EagI C'GGCC_G
Cuts at: 2009 2009
Size: 4424
EarI CTCTTCn'nnn_
Cuts at: 818 2447 2657 818
Size: 1629 210 2585
Fragments arranged by size:
2585 1629 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 637 1320 2632 2942 3088 4234 637
Size: 683 1312 310 146 1146 827
Fragments arranged by size:
1312 1146 827 683 310 146
EcoICRI GAG'CTC
Cuts at: 1436 1436
Size: 4424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 356 1115 1244 2249 2681 3428 3696 165
Size: 191 759 129 1005 432 747 268 893
Fragments arranged by size:
1005 893 759 747 432 268 191 129
EcoO109I rG'GnC_Cy
Cuts at: 119 149 279 939 1370 1563 119
Size: 30 130 660 431 193 2980
Fragments arranged by size:
2980 660 431 193 130 30
EcoRI G'AATT_C
Cuts at: 518 1428 518
Size: 910 3514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 960 984 1191 2443 960
Size: 24 207 1252 2941
Fragments arranged by size:
2941 1252 207 24
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 916 1858 2106 3128 4107 916
Size: 942 248 1022 979 1233
Fragments arranged by size:
1233 1022 979 942 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 1048 1080 1110 1142 1217 1249 1276
Size: 32 16 32 30 32 75 32 27
Cuts at: 1276 1308 2266 2296 2298 2328 1000
Size: 32 958 30 2 30 3096
Fragments arranged by size:
3096 958 75 32 32 32 32 32
30 30 30 27 16 2
HincII GTy'rAC
Cuts at: 1463 1463
Size: 4424
KasI G'GCGC_C
Cuts at: 1854 2102 1854
Size: 248 4176
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 956 3095 3279 956
Size: 2139 184 2101
Fragments arranged by size:
2139 2101 184
MscI TGG'CCA
Cuts at: 230 2185 230
Size: 1955 2469
MslI CAynn'nnrTG
Cuts at: 1138 1299 2413 2540 1138
Size: 161 1114 127 3022
Fragments arranged by size:
3022 1114 161 127
MspA1I CmG'CkG
Cuts at: 182 674 1263 1535 1849 2209 3222 3467 182
Size: 492 589 272 314 360 1013 245 1139
Fragments arranged by size:
1139 1013 589 492 360 314 272 245
NaeI GCC'GGC
Cuts at: 273 2605 273
Size: 2332 2092
NarI GG'CG_CC
Cuts at: 1855 2103 1855
Size: 248 4176
NcoI C'CATG_G
Cuts at: 107 107
Size: 4424
NgoMIV G'CCGG_C
Cuts at: 271 2603 271
Size: 2332 2092
NspI r_CATG'y
Cuts at: 1384 1477 1977 2884 1384
Size: 93 500 907 2924
Fragments arranged by size:
2924 907 500 93
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PflMI CCAn_nnn'nTGG
Cuts at: 443 674 1152 1341 1383 443
Size: 231 478 189 42 3484
Fragments arranged by size:
3484 478 231 189 42
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PpuMI rG'GwC_Cy
Cuts at: 119 279 939 119
Size: 160 660 3604
Fragments arranged by size:
3604 660 160
PstI C_TGCA'G
Cuts at: 1471 2156 1471
Size: 685 3739
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 1849 2209 1849
Size: 360 4064
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1438 1438
Size: 4424
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SanDI GG'GwC_CC
Cuts at: 119 119
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 2447 2657 2447
Size: 210 4214
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 369 997 1354 3941 369
Size: 628 357 2587 852
Fragments arranged by size:
2587 852 628 357
SfcI C'TryA_G
Cuts at: 33 1467 2152 3145 3336 33
Size: 1434 685 993 191 1121
Fragments arranged by size:
1434 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1856 2104 1856
Size: 248 4176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 1446 1446
Size: 4424
SmlI C'TyrA_G
Cuts at: 1579 2494 2986 3248 1579
Size: 915 492 262 2755
Fragments arranged by size:
2755 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 1477 1977 1477
Size: 500 3924
StuI AGG'CCT
Cuts at: 655 655
Size: 4424
StyI C'CwwG_G
Cuts at: 107 458 929 935 1558 1912 107
Size: 351 471 6 623 354 2619
Fragments arranged by size:
2619 623 471 354 351 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 435 2007 2140 2599 2808 390
Size: 45 1572 133 459 209 2006
Fragments arranged by size:
2006 1572 459 209 133 45
TatI w'GTAC_w
Cuts at: 125 176 367 995 1352 3939 125
Size: 51 191 628 357 2587 610
Fragments arranged by size:
2587 628 610 357 191 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 203 235 296 328 3941 3973 203
Size: 32 61 32 3613 32 654
Fragments arranged by size:
3613 654 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2221 2221
Size: 4424
XbaI T'CTAG_A
Cuts at: 633 1455 633
Size: 822 3602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 223 440 1919 223
Size: 217 1479 2728
Fragments arranged by size:
2728 1479 217
XmaI C'CCGG_G
Cuts at: 1444 1444
Size: 4424
XmnI GAAnn'nnTTC
Cuts at: 1174 1174
Size: 4424
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI