Restriction map for
pF1KB0973
(Circular) (Six-base) MAPSORT of: pF1KB0973.seq Check: 2242 from: 1 to: 3743
pF1KB0973 3743 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1568 2000 2747 3015 508
Size: 1060 432 747 268 1236
Fragments arranged by size:
1236 1060 747 432 268
AflIII A'CryG_T
Cuts at: 1095 2199 3176 1095
Size: 1104 977 1662
Fragments arranged by size:
1662 1104 977
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 1048 2615 1048
Size: 1567 2176
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 478 763 478
Size: 285 3458
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 653 759 1173 1421 1456 653
Size: 106 414 248 35 2940
Fragments arranged by size:
2940 414 248 106 35
BanII G_rGCy'C
Cuts at: 235 344 757 1787 235
Size: 109 413 1030 2191
Fragments arranged by size:
2191 1030 413 109
BbeI G_GCGC'C
Cuts at: 657 1177 1425 657
Size: 520 248 2975
Fragments arranged by size:
2975 520 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 131 165 90
Size: 34 7 34 3668
Fragments arranged by size:
3668 34 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglI GCCn_nnn'nGGC
Cuts at: 688 688
Size: 3743
BglII A'GATC_T
Cuts at: 551 1258 3295 551
Size: 707 2037 999
Fragments arranged by size:
2037 999 707
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 666 1368 1461 2517 666
Size: 702 93 1056 1892
Fragments arranged by size:
1892 1056 702 93
BmgBI CAC'GTC
Cuts at: 221 221
Size: 3743
BmrI ACTGGGnnnn_n'
Cuts at: 130 955 1036 1371 3515 130
Size: 825 81 335 2144 358
Fragments arranged by size:
2144 825 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 919 1798 2290 2588 661
Size: 258 879 492 298 1816
Fragments arranged by size:
1816 879 492 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 278 493 539 3447 278
Size: 215 46 2908 574
Fragments arranged by size:
2908 574 215 46
BsaAI yAC'GTr
Cuts at: 705 1096 705
Size: 391 3352
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 654 1174 1422 2124 3620 654
Size: 520 248 702 1496 777
Fragments arranged by size:
1496 777 702 520 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 538
Size: 30 3713
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 214
Size: 3743
BseYI C'CCAG_C
Cuts at: 1168 1926 2503 2999 1168
Size: 758 577 496 1912
Fragments arranged by size:
1912 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2539 104
Size: 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46
BsiHKAI G_wGCw'C
Cuts at: 757 1535 1725 2517 757
Size: 778 190 792 1983
Fragments arranged by size:
1983 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 211 211
Size: 3743
Bsp1286I G_dGCh'C
Cuts at: 235 344 666 757 1368 1461 1535 1725 1787
Size: 109 322 91 611 93 74 190 62
Cuts at: 1787 2517 235
Size: 730 1461
Fragments arranged by size:
1461 730 611 322 190 109 93 91
74 62
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 581 793 1007 1309 1690 581
Size: 212 214 302 381 2634
Fragments arranged by size:
2634 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 1741 1922 2131 1741
Size: 181 209 3353
Fragments arranged by size:
3353 209 181
BssSI C'ACGA_G
Cuts at: 2014 2372 2014
Size: 358 3385
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstEII G'GTnAC_C
Cuts at: 207 207
Size: 3743
BstYI r'GATC_y
Cuts at: 1 551 768 1235 1258 1593 1839 2851 2937
Size: 550 217 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 550 467 449 346 335 246 217
86 23 12
BtgI C'CryG_G
Cuts at: 122 212 122
Size: 90 3653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 472 986 472
Size: 514 3229
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 507 1449 2307 507
Size: 942 858 1943
Fragments arranged by size:
1943 942 858
EaeI y'GGCC_r
Cuts at: 1328 1502 1893 1920 3265 1328
Size: 174 391 27 1345 1806
Fragments arranged by size:
1806 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1951 2261 2407 3553 1951
Size: 310 146 1146 2141
Fragments arranged by size:
2141 1146 310 146
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1568 2000 2747 3015 508
Size: 1060 432 747 268 1236
Fragments arranged by size:
1236 1060 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 402 600 882 402
Size: 198 282 3263
Fragments arranged by size:
3263 282 198
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 163 1524 163
Size: 1361 2382
HaeII r_GCGC'y
Cuts at: 657 1177 1425 2447 3426 657
Size: 520 248 1022 979 974
Fragments arranged by size:
1022 979 974 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 536 538 568 570 1585 1615 1617 1647 536
Size: 2 30 2 1015 30 2 30 2632
Fragments arranged by size:
2632 1015 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 653 1173 1421 653
Size: 520 248 2975
Fragments arranged by size:
2975 520 248
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 1732 1859 1732
Size: 127 3616
MspA1I CmG'CkG
Cuts at: 149 410 854 1168 1528 2541 2786 149
Size: 261 444 314 360 1013 245 1106
Fragments arranged by size:
1106 1013 444 360 314 261 245
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 654 1174 1422 654
Size: 520 248 2975
Fragments arranged by size:
2975 520 248
NdeI CA'TA_TG
Cuts at: 645 645
Size: 3743
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NspI r_CATG'y
Cuts at: 168 796 1296 2203 168
Size: 628 500 907 1708
Fragments arranged by size:
1708 907 628 500
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PfoI T'CCnGG_A
Cuts at: 275 275
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PpuMI rG'GwC_Cy
Cuts at: 402 600 402
Size: 198 3545
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3058
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3383
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 786 1471 2464 2655 33
Size: 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 688 688
Size: 3743
SfoI GGC'GCC
Cuts at: 655 1175 1423 655
Size: 520 248 2975
Fragments arranged by size:
2975 520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 480 765 480
Size: 285 3458
SmlI C'TyrA_G
Cuts at: 676 898 1813 2305 2567 676
Size: 222 915 492 262 1852
Fragments arranged by size:
1852 915 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 168 796 1296 168
Size: 628 500 2615
Fragments arranged by size:
2615 628 500
SrfI GCCC'GGGC
Cuts at: 480 480
Size: 3743
StuI AGG'CCT
Cuts at: 682 682
Size: 3743
StyI C'CwwG_G
Cuts at: 336 877 1231 336
Size: 541 354 2848
Fragments arranged by size:
2848 541 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1326 1459 1918 2127 1326
Size: 133 459 209 2942
Fragments arranged by size:
2942 459 209 133
TatI w'GTAC_w
Cuts at: 3258 3258
Size: 3743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 3711
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XmaI C'CCGG_G
Cuts at: 478 763 478
Size: 285 3458
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI