Restriction map for
pF1KB0979
(Circular) (Six-base) MAPSORT of: pF1KB0979.seq Check: 4168 from: 1 to: 3803
pF1KB0979 3803 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 841 841
Size: 3803
Acc65I G'GTAC_C
Cuts at: 819 819
Size: 3803
AclI AA'CG_TT
Cuts at: 3643 3643
Size: 3803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 755 1628 2060 2807 3075 500
Size: 255 873 432 747 268 1228
Fragments arranged by size:
1228 873 747 432 268 255
AflIII A'CryG_T
Cuts at: 174 1155 2259 3236 174
Size: 981 1104 977 741
Fragments arranged by size:
1104 981 977 741
AgeI A'CCGG_T
Cuts at: 2191 2191
Size: 3803
AlwNI CAG_nnn'CTG
Cuts at: 1108 2675 1108
Size: 1567 2236
ApaLI G'TGCA_C
Cuts at: 2573 2573
Size: 3803
ApoI r'AATT_y
Cuts at: 119 217 402 807 3156 3412 119
Size: 98 185 405 2349 256 510
Fragments arranged by size:
2349 510 405 256 185 98
AseI AT'TA_AT
Cuts at: 20 20
Size: 3803
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
AvaI C'yCGr_G
Cuts at: 823 823
Size: 3803
BamHI G'GATC_C
Cuts at: 828 828
Size: 3803
BanI G'GyrC_C
Cuts at: 819 1233 1481 1516 819
Size: 414 248 35 3106
Fragments arranged by size:
3106 414 248 35
BanII G_rGCy'C
Cuts at: 472 817 1847 472
Size: 345 1030 2428
Fragments arranged by size:
2428 1030 345
BbeI G_GCGC'C
Cuts at: 1237 1485 1237
Size: 248 3555
BciVI GTATCCnnnnn_n'
Cuts at: 1701 2462 3803 1701
Size: 761 1341 1701
Fragments arranged by size:
1701 1341 761
BclI T'GATC_A
Cuts at: 1323 1323
Size: 3803
BglI GCCn_nnn'nGGC
Cuts at: 792 792
Size: 3803
BglII A'GATC_T
Cuts at: 1318 3355 1318
Size: 2037 1766
BlpI GC'TnA_GC
Cuts at: 236 915 236
Size: 679 3124
Bme1580I G_kGCm'C
Cuts at: 1428 1521 2577 1428
Size: 93 1056 2654
Fragments arranged by size:
2654 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1015 1096 1431 3575 1015
Size: 81 335 2144 1243
Fragments arranged by size:
2144 1243 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 216 2170 216
Size: 1954 1849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 716
Size: 3803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1858 2350 2648 979
Size: 879 492 298 2134
Fragments arranged by size:
2134 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3507 3507
Size: 3803
BsaAI yAC'GTr
Cuts at: 1156 1156
Size: 3803
BsaBI GATnn'nnATC
Cuts at: 6 1317 3354 6
Size: 1311 2037 455
Fragments arranged by size:
2037 1311 455
BsaHI Gr'CG_yC
Cuts at: 1234 1482 2184 3680 1234
Size: 248 702 1496 1357
Fragments arranged by size:
1496 1357 702 248
BsaWI w'CCGG_w
Cuts at: 992 1513 2191 2465 2612 3023 992
Size: 521 678 274 147 411 1772
Fragments arranged by size:
1772 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 577 580 577
Size: 3 3800
BseYI C'CCAG_C
Cuts at: 1228 1986 2563 3059 1228
Size: 758 577 496 1972
Fragments arranged by size:
1972 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1345 1391 2599 104
Size: 1241 46 1208 1308
Fragments arranged by size:
1308 1241 1208 46
BsiHKAI G_wGCw'C
Cuts at: 472 618 817 1595 1785 2577 472
Size: 146 199 778 190 792 1698
Fragments arranged by size:
1698 792 778 199 190 146
Bsp1286I G_dGCh'C
Cuts at: 472 618 817 1428 1521 1595 1785 1847 2577
Size: 146 199 611 93 74 190 62 730
Cuts at: 2577 472
Size: 1698
Fragments arranged by size:
1698 730 611 199 190 146 93 74
62
BspEI T'CCGG_A
Cuts at: 3023 3023
Size: 3803
BspHI T'CATG_A
Cuts at: 398 2979 398
Size: 2581 1222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 853 1067 1369 1750 853
Size: 214 302 381 2906
Fragments arranged by size:
2906 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2095 2149 3633 3798 50
Size: 2045 54 1484 165 55
Fragments arranged by size:
2045 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1715 1715
Size: 3803
BsrFI r'CCGG_y
Cuts at: 1801 1982 2191 1801
Size: 181 209 3413
Fragments arranged by size:
3413 209 181
BssSI C'ACGA_G
Cuts at: 2074 2432 2074
Size: 358 3445
BstAPI GCAn_nnn'nTGC
Cuts at: 1180 1180
Size: 3803
BstBI TT'CG_AA
Cuts at: 2164 2164
Size: 3803
BstYI r'GATC_y
Cuts at: 1 828 1295 1318 1653 1899 2911 2997 3009
Size: 827 467 23 335 246 1012 86 12
Cuts at: 3009 3355 1
Size: 346 449
Fragments arranged by size:
1012 827 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3803
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1753 1934 2097 1753
Size: 181 163 3459
Fragments arranged by size:
3459 181 163
BtsI GCAGTG_nn'
Cuts at: 378 1046 378
Size: 668 3135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 354 389 354
Size: 35 3768
DraI TTT'AAA
Cuts at: 802 802
Size: 3803
DrdI GACnn_nn'nnGTC
Cuts at: 1509 2367 1509
Size: 858 2945
EaeI y'GGCC_r
Cuts at: 270 1388 1562 1953 1980 3325 270
Size: 1118 174 391 27 1345 748
Fragments arranged by size:
1345 1118 748 391 174 27
EagI C'GGCC_G
Cuts at: 1388 1388
Size: 3803
EarI CTCTTCn'nnn_
Cuts at: 1826 2036 1826
Size: 210 3593
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2011 2321 2467 3613 2011
Size: 310 146 1146 2201
Fragments arranged by size:
2201 1146 310 146
EcoICRI GAG'CTC
Cuts at: 470 815 470
Size: 345 3458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 716 755 1628 2060 2807 3075 500
Size: 216 39 873 432 747 268 1228
Fragments arranged by size:
1228 873 747 432 268 216 39
EcoO109I rG'GnC_Cy
Cuts at: 942 942
Size: 3803
EcoRI G'AATT_C
Cuts at: 807 807
Size: 3803
EcoRV GAT'ATC
Cuts at: 435 435
Size: 3803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 238 1822 238
Size: 1584 2219
FspI TGC'GCA
Cuts at: 1584 1584
Size: 3803
HaeII r_GCGC'y
Cuts at: 1237 1485 2507 3486 1237
Size: 248 1022 979 1554
Fragments arranged by size:
1554 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 1645 1675 1677 1707 481
Size: 32 1132 30 2 30 2577
Fragments arranged by size:
2577 1132 32 30 30 2
HincII GTy'rAC
Cuts at: 842 842
Size: 3803
KasI G'GCGC_C
Cuts at: 1233 1481 1233
Size: 248 3555
KpnI G_GTAC'C
Cuts at: 823 823
Size: 3803
MluI A'CGCG_T
Cuts at: 3236 3236
Size: 3803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2474 2658 2474
Size: 184 3619
MscI TGG'CCA
Cuts at: 272 1564 272
Size: 1292 2511
MslI CAynn'nnrTG
Cuts at: 1792 1919 1792
Size: 127 3676
MspA1I CmG'CkG
Cuts at: 552 914 1228 1588 2601 2846 552
Size: 362 314 360 1013 245 1509
Fragments arranged by size:
1509 1013 362 360 314 245
NaeI GCC'GGC
Cuts at: 1984 1984
Size: 3803
NarI GG'CG_CC
Cuts at: 1234 1482 1234
Size: 248 3555
NcoI C'CATG_G
Cuts at: 107 107
Size: 3803
NgoMIV G'CCGG_C
Cuts at: 1982 1982
Size: 3803
NspI r_CATG'y
Cuts at: 178 856 1356 2263 178
Size: 678 500 907 1718
Fragments arranged by size:
1718 907 678 500
PciI A'CATG_T
Cuts at: 174 2259 174
Size: 2085 1718
PmeI GTTT'AAAC
Cuts at: 802 802
Size: 3803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 3771
PsiI TTA'TAA
Cuts at: 428 428
Size: 3803
PstI C_TGCA'G
Cuts at: 850 1535 850
Size: 685 3118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3803
PvuII CAG'CTG
Cuts at: 552 1228 1588 552
Size: 676 360 2767
Fragments arranged by size:
2767 676 360
RsrII CG'GwC_CG
Cuts at: 1998 1998
Size: 3803
SacI G_AGCT'C
Cuts at: 472 817 472
Size: 345 3458
SalI G'TCGA_C
Cuts at: 840 840
Size: 3803
SapI GCTCTTCn'nnn_
Cuts at: 1826 2036 1826
Size: 210 3593
SbfI CC_TGCA'GG
Cuts at: 850 850
Size: 3803
ScaI AGT'ACT
Cuts at: 3320 3320
Size: 3803
SfcI C'TryA_G
Cuts at: 33 685 846 1531 2524 2715 33
Size: 652 161 685 993 191 1121
Fragments arranged by size:
1121 993 685 652 191 161
SfoI GGC'GCC
Cuts at: 1235 1483 1235
Size: 248 3555
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
SmaI CCC'GGG
Cuts at: 825 825
Size: 3803
SmlI C'TyrA_G
Cuts at: 958 1873 2365 2627 958
Size: 915 492 262 2134
Fragments arranged by size:
2134 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3803
SphI G_CATG'C
Cuts at: 856 1356 856
Size: 500 3303
StyI C'CwwG_G
Cuts at: 107 937 1291 107
Size: 830 354 2619
Fragments arranged by size:
2619 830 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 702 744 1386 1519 1978 2187 571
Size: 131 42 642 133 459 209 2187
Fragments arranged by size:
2187 642 459 209 133 131 42
TatI w'GTAC_w
Cuts at: 3318 3318
Size: 3803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 3771
Tth111I GACn'n_nGTC
Cuts at: 1600 1600
Size: 3803
XbaI T'CTAG_A
Cuts at: 834 834
Size: 3803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1298 1298
Size: 3803
XmaI C'CCGG_G
Cuts at: 823 823
Size: 3803
XmnI GAAnn'nnTTC
Cuts at: 218 218
Size: 3803
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI