Restriction map for
pF1KB0982
(Circular) (Six-base) MAPSORT of: pF1KB0982.seq Check: 4079 from: 1 to: 4454
pF1KB0982 4454 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 680 1173 1492 680
Size: 493 319 3642
Fragments arranged by size:
3642 493 319
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4454
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1019 1248 2279 2711 3458 3726 914
Size: 105 229 1031 432 747 268 1642
Fragments arranged by size:
1642 1031 747 432 268 229 105
AfeI AGC'GCT
Cuts at: 1201 1201
Size: 4454
AflIII A'CryG_T
Cuts at: 1806 2910 3887 1806
Size: 1104 977 2373
Fragments arranged by size:
2373 1104 977
AgeI A'CCGG_T
Cuts at: 2842 2842
Size: 4454
AleI CACnn'nnGTG
Cuts at: 1210 1210
Size: 4454
AlwNI CAG_nnn'CTG
Cuts at: 252 1759 3326 252
Size: 1507 1567 1380
Fragments arranged by size:
1567 1507 1380
ApaI G_GGCC'C
Cuts at: 574 574
Size: 4454
ApaLI G'TGCA_C
Cuts at: 409 3224 409
Size: 2815 1639
ApoI r'AATT_y
Cuts at: 1458 3807 4063 1458
Size: 2349 256 1849
Fragments arranged by size:
2349 1849 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 908 1097 1190 1474 908
Size: 189 93 284 3888
Fragments arranged by size:
3888 284 189 93
BamHI G'GATC_C
Cuts at: 1415 1479 1415
Size: 64 4390
BanI G'GyrC_C
Cuts at: 587 1305 1470 1884 2132 2167 587
Size: 718 165 414 248 35 2874
Fragments arranged by size:
2874 718 414 248 165 35
BanII G_rGCy'C
Cuts at: 574 1096 1468 2498 574
Size: 522 372 1030 2530
Fragments arranged by size:
2530 1030 522 372
BbeI G_GCGC'C
Cuts at: 591 1888 2136 591
Size: 1297 248 2909
Fragments arranged by size:
2909 1297 248
BbvCI CC'TCA_GC
Cuts at: 798 1059 798
Size: 261 4193
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3113 4454 2352
Size: 761 1341 2352
Fragments arranged by size:
2352 1341 761
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BglI GCCn_nnn'nGGC
Cuts at: 145 154 585 826 145
Size: 9 431 241 3773
Fragments arranged by size:
3773 431 241 9
BglII A'GATC_T
Cuts at: 1969 4006 1969
Size: 2037 2417
BlpI GC'TnA_GC
Cuts at: 1566 1566
Size: 4454
Bme1580I G_kGCm'C
Cuts at: 413 574 1310 2079 2172 3228 413
Size: 161 736 769 93 1056 1639
Fragments arranged by size:
1639 1056 769 736 161 93
BmgBI CAC'GTC
Cuts at: 384 384
Size: 4454
BmrI ACTGGGnnnn_n'
Cuts at: 450 588 875 911 917 1666 1747 2082 4226
Size: 138 287 36 6 749 81 335 2144
Cuts at: 4226 450
Size: 678
Fragments arranged by size:
2144 749 678 335 287 138 81 36
6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 2821 486
Size: 2335 2119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 618 968 481
Size: 137 350 3967
Fragments arranged by size:
3967 350 137
Bpu10I CC'TnA_GC
Cuts at: 798 1059 798
Size: 261 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3001 3299 1630
Size: 879 492 298 2785
Fragments arranged by size:
2785 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 880 4158 880
Size: 3278 1176
BsaAI yAC'GTr
Cuts at: 356 1117 1807 356
Size: 761 690 3003
Fragments arranged by size:
3003 761 690
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 588 1170 1885 2133 2835 4331 588
Size: 582 715 248 702 1496 711
Fragments arranged by size:
1496 715 711 702 582 248
BsaWI w'CCGG_w
Cuts at: 1409 1643 2164 2842 3116 3263 3674 1409
Size: 234 521 678 274 147 411 2189
Fragments arranged by size:
2189 678 521 411 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 604 634 1009 1039 112
Size: 30 462 30 375 30 3527
Fragments arranged by size:
3527 462 375 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 892 1018 1021 1024 1027 1030 892
Size: 126 3 3 3 3 4316
Fragments arranged by size:
4316 126 3 3 3 3
BseYI C'CCAG_C
Cuts at: 330 575 1879 2637 3214 3710 330
Size: 245 1304 758 577 496 1074
Fragments arranged by size:
1304 1074 758 577 496 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 378 154
Size: 224 4230
BsiEI CG_ry'CG
Cuts at: 104 1996 2042 3250 104
Size: 1892 46 1208 1308
Fragments arranged by size:
1892 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 389 413 1130 1244 1468 2246 2436 3228 389
Size: 24 717 114 224 778 190 792 1615
Fragments arranged by size:
1615 792 778 717 224 190 114 24
Bsp1286I G_dGCh'C
Cuts at: 389 413 574 1096 1130 1244 1310 1468 2079
Size: 24 161 522 34 114 66 158 611
Cuts at: 2079 2172 2246 2436 2498 3228 389
Size: 93 74 190 62 730 1615
Fragments arranged by size:
1615 730 611 522 190 161 158 114
93 74 66 62 34 24
BspEI T'CCGG_A
Cuts at: 1409 3674 1409
Size: 2265 2189
BspHI T'CATG_A
Cuts at: 668 3630 668
Size: 2962 1492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 1504 1718 2020 2401 692
Size: 812 214 302 381 2745
Fragments arranged by size:
2745 812 381 302 214
BsrBI CCG'CTC
Cuts at: 50 230 2746 2800 4284 4449 50
Size: 180 2516 54 1484 165 55
Fragments arranged by size:
2516 1484 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2366 2366
Size: 4454
BsrFI r'CCGG_y
Cuts at: 2452 2633 2842 2452
Size: 181 209 4064
Fragments arranged by size:
4064 209 181
BssHII G'CGCG_C
Cuts at: 237 455 237
Size: 218 4236
BssSI C'ACGA_G
Cuts at: 2725 3083 2725
Size: 358 4096
BstAPI GCAn_nnn'nTGC
Cuts at: 359 497 1831 359
Size: 138 1334 2982
Fragments arranged by size:
2982 1334 138
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 429 926 429
Size: 497 3957
BstYI r'GATC_y
Cuts at: 1 643 1415 1479 1946 1969 2304 2550 3562
Size: 642 772 64 467 23 335 246 1012
Cuts at: 3562 3648 3660 4006 1
Size: 86 12 346 449
Fragments arranged by size:
1012 772 642 467 449 346 335 246
86 64 23 12
BtgI C'CryG_G
Cuts at: 107 780 1311 1429 107
Size: 673 531 118 3132
Fragments arranged by size:
3132 673 531 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 507 2404 2585 2748 507
Size: 1897 181 163 2213
Fragments arranged by size:
2213 1897 181 163
BtsI GCAGTG_nn'
Cuts at: 1046 1168 1296 1697 1046
Size: 122 128 401 3803
Fragments arranged by size:
3803 401 128 122
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DraIII CAC_nnn'GTG
Cuts at: 359 515 359
Size: 156 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 146 158 269 290 317 1106 1119 2039 2213
Size: 12 111 21 27 789 13 920 174
Cuts at: 2213 2604 2631 3976 146
Size: 391 27 1345 624
Fragments arranged by size:
1345 920 789 624 391 174 111 27
27 21 13 12
EagI C'GGCC_G
Cuts at: 2039 2039
Size: 4454
EarI CTCTTCn'nnn_
Cuts at: 394 995 1016 1324 2477 2687 394
Size: 601 21 308 1153 210 2161
Fragments arranged by size:
2161 1153 601 308 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1387 2662 2972 3118 4264 1387
Size: 1275 310 146 1146 1577
Fragments arranged by size:
1577 1275 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1466 1466
Size: 4454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 618 914 968 1019 1248 2279 2711 3458
Size: 137 296 54 51 229 1031 432 747
Cuts at: 3458 3726 481
Size: 268 1209
Fragments arranged by size:
1209 1031 747 432 296 268 229 137
54 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1410 1410
Size: 4454
EcoO109I rG'GnC_Cy
Cuts at: 120 570 571 724 742 905 1593 120
Size: 450 1 153 18 163 688 2981
Fragments arranged by size:
2981 688 450 163 153 18 1
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FspI TGC'GCA
Cuts at: 688 2235 688
Size: 1547 2907
HaeII r_GCGC'y
Cuts at: 379 591 1203 1888 2136 3158 4137 379
Size: 212 612 685 248 1022 979 696
Fragments arranged by size:
1022 979 696 685 612 248 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 2296 2326 2328 2358 112
Size: 32 2152 30 2 30 2208
Fragments arranged by size:
2208 2152 32 30 30 2
HincII GTy'rAC
Cuts at: 221 846 1174 1493 221
Size: 625 328 319 3182
Fragments arranged by size:
3182 625 328 319
KasI G'GCGC_C
Cuts at: 587 1884 2132 587
Size: 1297 248 2909
Fragments arranged by size:
2909 1297 248
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4454
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3125 3309 3125
Size: 184 4270
MscI TGG'CCA
Cuts at: 292 319 1108 1121 2215 292
Size: 27 789 13 1094 2531
Fragments arranged by size:
2531 1094 789 27 13
MslI CAynn'nnrTG
Cuts at: 286 1210 2443 2570 286
Size: 924 1233 127 2170
Fragments arranged by size:
2170 1233 924 127
MspA1I CmG'CkG
Cuts at: 145 1077 1185 1565 1879 2239 3252 3497 145
Size: 932 108 380 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 932 380 360 314 245 108
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 4454
NarI GG'CG_CC
Cuts at: 588 1885 2133 588
Size: 1297 248 2909
Fragments arranged by size:
2909 1297 248
NcoI C'CATG_G
Cuts at: 107 780 1311 107
Size: 673 531 3250
Fragments arranged by size:
3250 673 531
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 4454
NspI r_CATG'y
Cuts at: 1507 2007 2914 1507
Size: 500 907 3047
Fragments arranged by size:
3047 907 500
PasI CC'CwG_GG
Cuts at: 728 728
Size: 4454
PciI A'CATG_T
Cuts at: 2910 2910
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PmlI CAC'GTG
Cuts at: 356 356
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4422
PpuMI rG'GwC_Cy
Cuts at: 120 724 905 120
Size: 604 181 3669
Fragments arranged by size:
3669 604 181
PspOMI G'GGCC_C
Cuts at: 570 570
Size: 4454
PstI C_TGCA'G
Cuts at: 1082 1501 2186 1082
Size: 419 685 3350
Fragments arranged by size:
3350 685 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4454
PvuII CAG'CTG
Cuts at: 1185 1879 2239 1185
Size: 694 360 3400
Fragments arranged by size:
3400 694 360
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 1468 1468
Size: 4454
SalI G'TCGA_C
Cuts at: 1172 1491 1172
Size: 319 4135
SanDI GG'GwC_CC
Cuts at: 905 905
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 394 2477 2687 394
Size: 2083 210 2161
Fragments arranged by size:
2161 2083 210
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SfcI C'TryA_G
Cuts at: 33 1078 1151 1269 1497 2182 3175 3366 33
Size: 1045 73 118 228 685 993 191 1121
Fragments arranged by size:
1121 1045 993 685 228 191 118 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 145 145
Size: 4454
SfoI GGC'GCC
Cuts at: 589 1886 2134 589
Size: 1297 248 2909
Fragments arranged by size:
2909 1297 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 910 1099 1192 1476 910
Size: 189 93 284 3888
Fragments arranged by size:
3888 284 189 93
SmlI C'TyrA_G
Cuts at: 1609 2524 3016 3278 1609
Size: 915 492 262 2785
Fragments arranged by size:
2785 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 1507 2007 1507
Size: 500 3954
StuI AGG'CCT
Cuts at: 616 616
Size: 4454
StyI C'CwwG_G
Cuts at: 107 734 780 1311 1588 1942 107
Size: 627 46 531 277 354 2619
Fragments arranged by size:
2619 627 531 354 277 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 936 1135 2037 2170 2629 2838 936
Size: 199 902 133 459 209 2552
Fragments arranged by size:
2552 902 459 209 199 133
TatI w'GTAC_w
Cuts at: 464 3969 464
Size: 3505 949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 1009 1041 3971 4003 844
Size: 32 133 32 2930 32 1295
Fragments arranged by size:
2930 1295 133 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4454
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 1949 327
Size: 1622 2832
XmaI C'CCGG_G
Cuts at: 908 1097 1190 1474 908
Size: 189 93 284 3888
Fragments arranged by size:
3888 284 189 93
XmnI GAAnn'nnTTC
Cuts at: 253 1201 253
Size: 948 3506
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PshAI PsiI PspXI PsrI
SacII SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI