Restriction map for
pF1KB0987
(Circular) (Six-base) MAPSORT of: pF1KB0987.seq Check: 1539 from: 1 to: 3887
pF1KB0987 3887 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 557 925 557
Size: 368 3519
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 487 790 847 1712 2144 2891 3159 190
Size: 297 303 57 865 432 747 268 918
Fragments arranged by size:
918 865 747 432 303 297 268 57
AflIII A'CryG_T
Cuts at: 404 1239 2343 3320 404
Size: 835 1104 977 971
Fragments arranged by size:
1104 977 971 835
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 270 302 270
Size: 32 3855
AlwNI CAG_nnn'CTG
Cuts at: 250 350 543 642 1192 2759 250
Size: 100 193 99 550 1567 1378
Fragments arranged by size:
1567 1378 550 193 100 99
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 907 907
Size: 3887
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 622 903 1317 1565 1600 622
Size: 281 414 248 35 2909
Fragments arranged by size:
2909 414 281 248 35
BanII G_rGCy'C
Cuts at: 901 1931 901
Size: 1030 2857
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BbsI GAAGACnn'nnnn_
Cuts at: 249 249
Size: 3887
BbvCI CC'TCA_GC
Cuts at: 243 439 632 243
Size: 196 193 3498
Fragments arranged by size:
3498 196 193
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 518 999 518
Size: 481 3406
Bme1580I G_kGCm'C
Cuts at: 627 1512 1605 2661 627
Size: 885 93 1056 1853
Fragments arranged by size:
1853 1056 885 93
BmrI ACTGGGnnnn_n'
Cuts at: 825 1099 1180 1515 3659 825
Size: 274 81 335 2144 1053
Fragments arranged by size:
2144 1053 335 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 258 2254 258
Size: 1996 1891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 673 678 598
Size: 75 5 3807
Fragments arranged by size:
3807 75 5
Bpu10I CC'TnA_GC
Cuts at: 243 439 632 243
Size: 196 193 3498
Fragments arranged by size:
3498 196 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1063 1942 2434 2732 496
Size: 567 879 492 298 1651
Fragments arranged by size:
1651 879 567 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1597 2275 2549 2696 3107 1076
Size: 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 307 370 151
Size: 156 63 3668
Fragments arranged by size:
3668 156 63
BseYI C'CCAG_C
Cuts at: 583 618 642 1312 2070 2647 3143 583
Size: 35 24 670 758 577 496 1327
Fragments arranged by size:
1327 758 670 577 496 35 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 363
Size: 3887
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 187 659 804 901 1679 1869 2661 187
Size: 472 145 97 778 190 792 1413
Fragments arranged by size:
1413 792 778 472 190 145 97
BsmI GAATG_Cn'
Cuts at: 388 780 388
Size: 392 3495
Bsp1286I G_dGCh'C
Cuts at: 187 627 659 804 901 1512 1605 1679 1869
Size: 440 32 145 97 611 93 74 190
Cuts at: 1869 1931 2661 187
Size: 62 730 1413
Fragments arranged by size:
1413 730 611 440 190 145 97 93
74 62 32
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 414 3063 414
Size: 2649 1238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 335 937 1151 1453 1834 335
Size: 602 214 302 381 2388
Fragments arranged by size:
2388 602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 491 1799 491
Size: 1308 2579
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 191 543 1264 191
Size: 352 721 2814
Fragments arranged by size:
2814 721 352
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 561 561
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 643 1130 643
Size: 487 3400
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 674 743 1472 1646 2037 2064 3409 674
Size: 69 729 174 391 27 1345 1152
Fragments arranged by size:
1345 1152 729 391 174 69 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 208 328 1910 2120 208
Size: 120 1582 210 1975
Fragments arranged by size:
1975 1582 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 487 598 673 678 790 847 1712 2144
Size: 297 111 75 5 112 57 865 432
Cuts at: 2144 2891 3159 190
Size: 747 268 918
Fragments arranged by size:
918 865 747 432 297 268 112 111
75 57 5
EcoNI CCTnn'n_nnAGG
Cuts at: 172 172
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 1026 1026
Size: 3887
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 1321 1569 2591 3570 1321
Size: 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1729 1759 1761 1791 1729
Size: 30 2 30 3825
Fragments arranged by size:
3825 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 815 2558 2742 815
Size: 1743 184 1960
Fragments arranged by size:
1960 1743 184
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 1876 2003 1876
Size: 127 3760
MspA1I CmG'CkG
Cuts at: 507 618 651 998 1312 1672 2685 2930 507
Size: 111 33 347 314 360 1013 245 1464
Fragments arranged by size:
1464 1013 360 347 314 245 111 33
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NspI r_CATG'y
Cuts at: 232 408 663 940 1440 2347 232
Size: 176 255 277 500 907 1772
Fragments arranged by size:
1772 907 500 277 255 176
PciI A'CATG_T
Cuts at: 404 2343 404
Size: 1939 1948
PflMI CCAn_nnn'nTGG
Cuts at: 576 576
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PstI C_TGCA'G
Cuts at: 539 548 934 1619 539
Size: 9 386 685 2807
Fragments arranged by size:
2807 685 386 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 618 651 1312 1672 618
Size: 33 661 360 2833
Fragments arranged by size:
2833 661 360 33
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 535 544 930 1615 2608 2799 33
Size: 502 9 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 502 386 191 9
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 511 1042 1957 2449 2711 511
Size: 531 915 492 262 1687
Fragments arranged by size:
1687 915 531 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StuI AGG'CCT
Cuts at: 241 241
Size: 3887
StyI C'CwwG_G
Cuts at: 746 1021 1375 746
Size: 275 354 3258
Fragments arranged by size:
3258 354 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 1470 1603 2062 2271 610
Size: 860 133 459 209 2226
Fragments arranged by size:
2226 860 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 615 1382 615
Size: 767 3120
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
XmnI GAAnn'nnTTC
Cuts at: 857 857
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI