Restriction map for
pF1KB0989
(Circular) (Six-base) MAPSORT of: pF1KB0989.seq Check: 9398 from: 1 to: 3518
pF1KB0989 3518 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 556 556
Size: 3518
Acc65I G'GTAC_C
Cuts at: 534 534
Size: 3518
AclI AA'CG_TT
Cuts at: 3358 3358
Size: 3518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1343 1775 2522 2790 1343
Size: 432 747 268 2071
Fragments arranged by size:
2071 747 432 268
AflIII A'CryG_T
Cuts at: 870 1974 2951 870
Size: 1104 977 1437
Fragments arranged by size:
1437 1104 977
AgeI A'CCGG_T
Cuts at: 1906 1906
Size: 3518
AlwNI CAG_nnn'CTG
Cuts at: 823 2390 823
Size: 1567 1951
ApaI G_GGCC'C
Cuts at: 155 345 155
Size: 190 3328
ApaLI G'TGCA_C
Cuts at: 2288 2288
Size: 3518
ApoI r'AATT_y
Cuts at: 522 2871 3127 522
Size: 2349 256 913
Fragments arranged by size:
2349 913 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
AvaI C'yCGr_G
Cuts at: 166 338 345 538 166
Size: 172 7 193 3146
Fragments arranged by size:
3146 193 172 7
BamHI G'GATC_C
Cuts at: 543 543
Size: 3518
BanI G'GyrC_C
Cuts at: 126 534 948 1196 1231 126
Size: 408 414 248 35 2413
Fragments arranged by size:
2413 414 408 248 35
BanII G_rGCy'C
Cuts at: 155 173 323 345 532 1562 155
Size: 18 150 22 187 1030 2111
Fragments arranged by size:
2111 1030 187 150 22 18
BbeI G_GCGC'C
Cuts at: 952 1200 952
Size: 248 3270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3484
BciVI GTATCCnnnnn_n'
Cuts at: 1416 2177 3518 1416
Size: 761 1341 1416
Fragments arranged by size:
1416 1341 761
BclI T'GATC_A
Cuts at: 1038 1038
Size: 3518
BglII A'GATC_T
Cuts at: 1033 3070 1033
Size: 2037 1481
BlpI GC'TnA_GC
Cuts at: 203 490 630 203
Size: 287 140 3091
Fragments arranged by size:
3091 287 140
Bme1580I G_kGCm'C
Cuts at: 155 345 1143 1236 2292 155
Size: 190 798 93 1056 1381
Fragments arranged by size:
1381 1056 798 190 93
BmrI ACTGGGnnnn_n'
Cuts at: 730 811 1146 3290 730
Size: 81 335 2144 958
Fragments arranged by size:
2144 958 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1885 1885
Size: 3518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 3518
Bpu10I CC'TnA_GC
Cuts at: 147 147
Size: 3518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1573 2065 2363 694
Size: 879 492 298 1849
Fragments arranged by size:
1849 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3222 3222
Size: 3518
BsaAI yAC'GTr
Cuts at: 871 871
Size: 3518
BsaBI GATnn'nnATC
Cuts at: 6 1032 3069 6
Size: 1026 2037 455
Fragments arranged by size:
2037 1026 455
BsaHI Gr'CG_yC
Cuts at: 949 1197 1899 3395 949
Size: 248 702 1496 1072
Fragments arranged by size:
1496 1072 702 248
BsaWI w'CCGG_w
Cuts at: 707 1228 1906 2180 2327 2738 707
Size: 521 678 274 147 411 1487
Fragments arranged by size:
1487 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 310 340 310
Size: 30 3488
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 180 180
Size: 3518
BseYI C'CCAG_C
Cuts at: 224 943 1701 2278 2774 224
Size: 719 758 577 496 968
Fragments arranged by size:
968 758 719 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 380
Size: 3518
BsiEI CG_ry'CG
Cuts at: 104 1060 1106 2314 104
Size: 956 46 1208 1308
Fragments arranged by size:
1308 1208 956 46
BsiHKAI G_wGCw'C
Cuts at: 173 491 532 1310 1500 2292 173
Size: 318 41 778 190 792 1399
Fragments arranged by size:
1399 792 778 318 190 41
Bsp1286I G_dGCh'C
Cuts at: 155 173 323 345 491 532 1143 1236 1310
Size: 18 150 22 146 41 611 93 74
Cuts at: 1310 1500 1562 2292 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 730 611 190 150 146 93 74
62 41 22 18
BspEI T'CCGG_A
Cuts at: 2738 2738
Size: 3518
BspHI T'CATG_A
Cuts at: 2694 2694
Size: 3518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 568 782 1084 1465 568
Size: 214 302 381 2621
Fragments arranged by size:
2621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 202 1810 1864 3348 3513 50
Size: 152 1608 54 1484 165 55
Fragments arranged by size:
1608 1484 165 152 55 54
BsrDI GCAATG_nn'
Cuts at: 1430 1430
Size: 3518
BsrFI r'CCGG_y
Cuts at: 1516 1697 1906 1516
Size: 181 209 3128
Fragments arranged by size:
3128 209 181
BssSI C'ACGA_G
Cuts at: 1789 2147 1789
Size: 358 3160
BstAPI GCAn_nnn'nTGC
Cuts at: 895 895
Size: 3518
BstBI TT'CG_AA
Cuts at: 1879 1879
Size: 3518
BstYI r'GATC_y
Cuts at: 1 446 543 1010 1033 1368 1614 2626 2712
Size: 445 97 467 23 335 246 1012 86
Cuts at: 2712 2724 3070 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 246 97
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1468 1649 1812 1468
Size: 181 163 3174
Fragments arranged by size:
3174 181 163
BtsI GCAGTG_nn'
Cuts at: 761 761
Size: 3518
DraI TTT'AAA
Cuts at: 517 517
Size: 3518
DrdI GACnn_nn'nnGTC
Cuts at: 1224 2082 1224
Size: 858 2660
EaeI y'GGCC_r
Cuts at: 1103 1277 1668 1695 3040 1103
Size: 174 391 27 1345 1581
Fragments arranged by size:
1581 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1103 1103
Size: 3518
EarI CTCTTCn'nnn_
Cuts at: 301 1541 1751 301
Size: 1240 210 2068
Fragments arranged by size:
2068 1240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 315 439 1726 2036 2182 3328 315
Size: 124 1287 310 146 1146 505
Fragments arranged by size:
1287 1146 505 310 146 124
EcoICRI GAG'CTC
Cuts at: 171 530 171
Size: 359 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1343 1775 2522 2790 337
Size: 1006 432 747 268 1065
Fragments arranged by size:
1065 1006 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 151 152 159 342 657 151
Size: 1 7 183 315 3012
Fragments arranged by size:
3012 315 183 7 1
EcoRI G'AATT_C
Cuts at: 522 522
Size: 3518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1537 1537
Size: 3518
FspI TGC'GCA
Cuts at: 463 1299 463
Size: 836 2682
HaeII r_GCGC'y
Cuts at: 314 952 1200 2222 3201 314
Size: 638 248 1022 979 631
Fragments arranged by size:
1022 979 638 631 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 415 447 1360 1390 1392 1422 310
Size: 32 73 32 913 30 2 30 2406
Fragments arranged by size:
2406 913 73 32 32 30 30 2
HincII GTy'rAC
Cuts at: 557 557
Size: 3518
KasI G'GCGC_C
Cuts at: 948 1196 948
Size: 248 3270
KpnI G_GTAC'C
Cuts at: 538 538
Size: 3518
MluI A'CGCG_T
Cuts at: 2951 2951
Size: 3518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 198 2189 2373 198
Size: 1991 184 1343
Fragments arranged by size:
1991 1343 184
MscI TGG'CCA
Cuts at: 1279 1279
Size: 3518
MslI CAynn'nnrTG
Cuts at: 1507 1634 1507
Size: 127 3391
MspA1I CmG'CkG
Cuts at: 123 181 207 420 629 943 1303 2316 2561
Size: 58 26 213 209 314 360 1013 245
Cuts at: 2561 123
Size: 1080
Fragments arranged by size:
1080 1013 360 314 245 213 209 58
26
NaeI GCC'GGC
Cuts at: 1699 1699
Size: 3518
NarI GG'CG_CC
Cuts at: 949 1197 949
Size: 248 3270
NgoMIV G'CCGG_C
Cuts at: 1697 1697
Size: 3518
NspI r_CATG'y
Cuts at: 254 571 1071 1978 254
Size: 317 500 907 1794
Fragments arranged by size:
1794 907 500 317
PasI CC'CwG_GG
Cuts at: 155 212 155
Size: 57 3461
PciI A'CATG_T
Cuts at: 1974 1974
Size: 3518
PmeI GTTT'AAAC
Cuts at: 517 517
Size: 3518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2689 2657
Size: 32 3486
PspOMI G'GGCC_C
Cuts at: 151 341 151
Size: 190 3328
PstI C_TGCA'G
Cuts at: 565 1250 565
Size: 685 2833
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3518
PvuII CAG'CTG
Cuts at: 123 207 943 1303 123
Size: 84 736 360 2338
Fragments arranged by size:
2338 736 360 84
RsrII CG'GwC_CG
Cuts at: 1713 1713
Size: 3518
SacI G_AGCT'C
Cuts at: 173 532 173
Size: 359 3159
SalI G'TCGA_C
Cuts at: 555 555
Size: 3518
SapI GCTCTTCn'nnn_
Cuts at: 301 1541 1751 301
Size: 1240 210 2068
Fragments arranged by size:
2068 1240 210
SbfI CC_TGCA'GG
Cuts at: 565 565
Size: 3518
ScaI AGT'ACT
Cuts at: 3035 3035
Size: 3518
SfcI C'TryA_G
Cuts at: 33 561 1246 2239 2430 33
Size: 528 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 191
SfoI GGC'GCC
Cuts at: 950 1198 950
Size: 248 3270
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
SmaI CCC'GGG
Cuts at: 340 347 540 340
Size: 7 193 3318
Fragments arranged by size:
3318 193 7
SmlI C'TyrA_G
Cuts at: 673 1588 2080 2342 673
Size: 915 492 262 1849
Fragments arranged by size:
1849 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3518
SphI G_CATG'C
Cuts at: 571 1071 571
Size: 500 3018
StyI C'CwwG_G
Cuts at: 652 1006 652
Size: 354 3164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1101 1234 1693 1902 1101
Size: 133 459 209 2717
Fragments arranged by size:
2717 459 209 133
TatI w'GTAC_w
Cuts at: 407 3033 407
Size: 2626 892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3486
Tth111I GACn'n_nGTC
Cuts at: 1315 1315
Size: 3518
XbaI T'CTAG_A
Cuts at: 549 549
Size: 3518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1013 1013
Size: 3518
XmaI C'CCGG_G
Cuts at: 338 345 538 338
Size: 7 193 3318
Fragments arranged by size:
3318 193 7
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI