Restriction map for
pF1KB0995
(Circular) (Six-base) MAPSORT of: pF1KB0995.seq Check: 1009 from: 1 to: 3965
pF1KB0995 3965 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1003 1003
Size: 3965
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1790 2222 2969 3237 605
Size: 1185 432 747 268 1333
Fragments arranged by size:
1333 1185 747 432 268
AflIII A'CryG_T
Cuts at: 199 1317 2421 3398 199
Size: 1118 1104 977 766
Fragments arranged by size:
1118 1104 977 766
AgeI A'CCGG_T
Cuts at: 2353 2353
Size: 3965
AlwNI CAG_nnn'CTG
Cuts at: 1270 2837 1270
Size: 1567 2398
ApaLI G'TGCA_C
Cuts at: 312 375 2735 312
Size: 63 2360 1542
Fragments arranged by size:
2360 1542 63
ApoI r'AATT_y
Cuts at: 457 969 3318 3574 457
Size: 512 2349 256 848
Fragments arranged by size:
2349 848 512 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 985 985
Size: 3965
BamHI G'GATC_C
Cuts at: 990 990
Size: 3965
BanI G'GyrC_C
Cuts at: 808 981 1395 1643 1678 808
Size: 173 414 248 35 3095
Fragments arranged by size:
3095 414 248 173 35
BanII G_rGCy'C
Cuts at: 979 2009 979
Size: 1030 2935
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BglII A'GATC_T
Cuts at: 690 1480 3517 690
Size: 790 2037 1138
Fragments arranged by size:
2037 1138 790
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 316 379 1590 1683 2739 316
Size: 63 1211 93 1056 1542
Fragments arranged by size:
1542 1211 1056 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 1177 1258 1593 3737 1177
Size: 81 335 2144 1405
Fragments arranged by size:
2144 1405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 420 2332 420
Size: 1912 2053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2020 2512 2810 1141
Size: 879 492 298 2296
Fragments arranged by size:
2296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 3669 805
Size: 2864 1101
BsaAI yAC'GTr
Cuts at: 1318 1318
Size: 3965
BsaBI GATnn'nnATC
Cuts at: 6 1479 3516 6
Size: 1473 2037 455
Fragments arranged by size:
2037 1473 455
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 940 1154 1675 2353 2627 2774 3185 940
Size: 214 521 678 274 147 411 1720
Fragments arranged by size:
1720 678 521 411 274 214 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 385 415 385
Size: 30 3935
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 393 393
Size: 3965
BseYI C'CCAG_C
Cuts at: 1390 2148 2725 3221 1390
Size: 758 577 496 2134
Fragments arranged by size:
2134 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 123
Size: 3965
BsiEI CG_ry'CG
Cuts at: 104 1507 1553 2761 104
Size: 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 316 379 403 979 1757 1947 2739 316
Size: 63 24 576 778 190 792 1542
Fragments arranged by size:
1542 792 778 576 190 63 24
BsmI GAATG_Cn'
Cuts at: 241 856 241
Size: 615 3350
BsmBI CGTCTCn'nnnn_
Cuts at: 713 713
Size: 3965
Bsp1286I G_dGCh'C
Cuts at: 316 379 403 979 1590 1683 1757 1947 2009
Size: 63 24 576 611 93 74 190 62
Cuts at: 2009 2739 316
Size: 730 1542
Fragments arranged by size:
1542 730 611 576 190 93 74 63
62 24
BspEI T'CCGG_A
Cuts at: 940 3185 940
Size: 2245 1720
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1015 1229 1531 1912 1015
Size: 214 302 381 3068
Fragments arranged by size:
3068 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2257 2311 3795 3960 50
Size: 2207 54 1484 165 55
Fragments arranged by size:
2207 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1877 1877
Size: 3965
BsrFI r'CCGG_y
Cuts at: 284 1963 2144 2353 284
Size: 1679 181 209 1896
Fragments arranged by size:
1896 1679 209 181
BssSI C'ACGA_G
Cuts at: 2236 2594 2236
Size: 358 3607
BstAPI GCAn_nnn'nTGC
Cuts at: 320 383 743 1342 320
Size: 63 360 599 2943
Fragments arranged by size:
2943 599 360 63
BstBI TT'CG_AA
Cuts at: 2326 2326
Size: 3965
BstXI CCAn_nnnn'nTGG
Cuts at: 934 934
Size: 3965
BstYI r'GATC_y
Cuts at: 1 267 690 990 1457 1480 1815 2061 3073
Size: 266 423 300 467 23 335 246 1012
Cuts at: 3073 3159 3171 3517 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 423 346 335 300 266
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 417 1208 417
Size: 791 3174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 528 563 528
Size: 35 3930
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DraIII CAC_nnn'GTG
Cuts at: 383 383
Size: 3965
DrdI GACnn_nn'nnGTC
Cuts at: 1671 2529 1671
Size: 858 3107
EaeI y'GGCC_r
Cuts at: 1550 1724 2115 2142 3487 1550
Size: 174 391 27 1345 2028
Fragments arranged by size:
2028 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1550 1550
Size: 3965
EarI CTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2173 2483 2629 3775 2173
Size: 310 146 1146 2363
Fragments arranged by size:
2363 1146 310 146
EcoICRI GAG'CTC
Cuts at: 977 977
Size: 3965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1790 2222 2969 3237 605
Size: 1185 432 747 268 1333
Fragments arranged by size:
1333 1185 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1104 1104
Size: 3965
EcoRI G'AATT_C
Cuts at: 969 969
Size: 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 936 1984 936
Size: 1048 2917
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 369 1399 1647 2669 3648 369
Size: 1030 248 1022 979 686
Fragments arranged by size:
1030 1022 979 686 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 1807 1837 1839 1869 102
Size: 32 1673 30 2 30 2198
Fragments arranged by size:
2198 1673 32 30 30 2
HincII GTy'rAC
Cuts at: 1004 1004
Size: 3965
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MfeI C'AATT_G
Cuts at: 248 248
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2820 2636
Size: 184 3781
MscI TGG'CCA
Cuts at: 1726 1726
Size: 3965
MslI CAynn'nnrTG
Cuts at: 293 758 1954 2081 293
Size: 465 1196 127 2177
Fragments arranged by size:
2177 1196 465 127
MspA1I CmG'CkG
Cuts at: 1076 1390 1750 2763 3008 1076
Size: 314 360 1013 245 2033
Fragments arranged by size:
2033 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NspI r_CATG'y
Cuts at: 203 1018 1518 2425 203
Size: 815 500 907 1743
Fragments arranged by size:
1743 907 815 500
PciI A'CATG_T
Cuts at: 199 2421 199
Size: 2222 1743
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3933
PstI C_TGCA'G
Cuts at: 1012 1697 1012
Size: 685 3280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 1390 1750 1390
Size: 360 3605
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 979 979
Size: 3965
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 3482 3482
Size: 3965
SfcI C'TryA_G
Cuts at: 33 515 681 1008 1693 2686 2877 33
Size: 482 166 327 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 327 191 166
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 987 987
Size: 3965
SmlI C'TyrA_G
Cuts at: 1120 2035 2527 2789 1120
Size: 915 492 262 2296
Fragments arranged by size:
2296 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
StyI C'CwwG_G
Cuts at: 356 881 1099 1453 356
Size: 525 218 354 2868
Fragments arranged by size:
2868 525 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1548 1681 2140 2349 1548
Size: 133 459 209 3164
Fragments arranged by size:
3164 459 209 133
TatI w'GTAC_w
Cuts at: 168 3480 168
Size: 3312 653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 3933
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1460 1460
Size: 3965
XmaI C'CCGG_G
Cuts at: 985 985
Size: 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI