Restriction map for
pF1KB0997
(Circular) (Six-base) MAPSORT of: pF1KB0997.seq Check: 212 from: 1 to: 3932
pF1KB0997 3932 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 948 948
Size: 3932
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1757 2189 2936 3204 542
Size: 1215 432 747 268 1270
Fragments arranged by size:
1270 1215 747 432 268
AflIII A'CryG_T
Cuts at: 1284 2388 3365 1284
Size: 1104 977 1851
Fragments arranged by size:
1851 1104 977
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2804 1237
Size: 1567 2365
ApaLI G'TGCA_C
Cuts at: 2702 2702
Size: 3932
ApoI r'AATT_y
Cuts at: 293 936 3285 3541 293
Size: 643 2349 256 684
Fragments arranged by size:
2349 684 643 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 160 184 483 952 160
Size: 24 299 469 3140
Fragments arranged by size:
3140 469 299 24
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 909 948 1362 1610 1645 909
Size: 39 414 248 35 3196
Fragments arranged by size:
3196 414 248 39 35
BanII G_rGCy'C
Cuts at: 160 656 946 1976 160
Size: 496 290 1030 2116
Fragments arranged by size:
2116 1030 496 290
BbeI G_GCGC'C
Cuts at: 1366 1614 1366
Size: 248 3684
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 1452 1452
Size: 3932
BglII A'GATC_T
Cuts at: 882 1447 3484 882
Size: 565 2037 1330
Fragments arranged by size:
2037 1330 565
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 1557 1650 2706 1557
Size: 93 1056 2783
Fragments arranged by size:
2783 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 619 1144 1225 1560 3704 619
Size: 525 81 335 2144 847
Fragments arranged by size:
2144 847 525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 3932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 863 420
Size: 443 3489
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1987 2479 2777 1108
Size: 879 492 298 2263
Fragments arranged by size:
2263 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3636 3636
Size: 3932
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 1363 1611 2313 3809 1363
Size: 248 702 1496 1486
Fragments arranged by size:
1496 1486 702 248
BsaWI w'CCGG_w
Cuts at: 338 1121 1642 2320 2594 2741 3152 338
Size: 783 521 678 274 147 411 1118
Fragments arranged by size:
1118 783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 393 423 393
Size: 30 3902
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 864 867 864
Size: 3 3929
BseYI C'CCAG_C
Cuts at: 706 718 1357 2115 2692 3188 706
Size: 12 639 758 577 496 1450
Fragments arranged by size:
1450 758 639 577 496 12
BsiEI CG_ry'CG
Cuts at: 104 1474 1520 2728 104
Size: 1370 46 1208 1308
Fragments arranged by size:
1370 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 200 656 946 1724 1914 2706 200
Size: 456 290 778 190 792 1426
Fragments arranged by size:
1426 792 778 456 290 190
BsmBI CGTCTCn'nnnn_
Cuts at: 340 340
Size: 3932
Bsp1286I G_dGCh'C
Cuts at: 160 200 656 946 1557 1650 1724 1914 1976
Size: 40 456 290 611 93 74 190 62
Cuts at: 1976 2706 160
Size: 730 1386
Fragments arranged by size:
1386 730 611 456 290 190 93 74
62 40
BspEI T'CCGG_A
Cuts at: 338 3152 338
Size: 2814 1118
BspHI T'CATG_A
Cuts at: 326 3108 326
Size: 2782 1150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 982 1196 1498 1879 982
Size: 214 302 381 3035
Fragments arranged by size:
3035 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 1844 252
Size: 1592 2340
BsrFI r'CCGG_y
Cuts at: 888 1930 2111 2320 888
Size: 1042 181 209 2500
Fragments arranged by size:
2500 1042 209 181
BssSI C'ACGA_G
Cuts at: 378 2203 2561 378
Size: 1825 358 1749
Fragments arranged by size:
1825 1749 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1309 1309
Size: 3932
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstYI r'GATC_y
Cuts at: 1 882 957 1424 1447 1782 2028 3040 3126
Size: 881 75 467 23 335 246 1012 86
Cuts at: 3126 3138 3484 1
Size: 12 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 86
75 23 12
Bsu36I CC'TnA_GG
Cuts at: 713 713
Size: 3932
BtgI C'CryG_G
Cuts at: 151 427 151
Size: 276 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1882 2063 2226 1882
Size: 181 163 3588
Fragments arranged by size:
3588 181 163
BtsI GCAGTG_nn'
Cuts at: 683 1175 683
Size: 492 3440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 450 485 450
Size: 35 3897
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 800 1517 1691 2082 2109 3454 800
Size: 717 174 391 27 1345 1278
Fragments arranged by size:
1345 1278 717 391 174 27
EagI C'GGCC_G
Cuts at: 1517 1517
Size: 3932
EarI CTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 2450 2596 3742 2140
Size: 310 146 1146 2330
Fragments arranged by size:
2330 1146 310 146
EcoICRI GAG'CTC
Cuts at: 654 944 654
Size: 290 3642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 542 863 1757 2189 2936 3204 420
Size: 122 321 894 432 747 268 1148
Fragments arranged by size:
1148 894 747 432 321 268 122
EcoO109I rG'GnC_Cy
Cuts at: 188 423 1071 188
Size: 235 648 3049
Fragments arranged by size:
3049 648 235
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
EcoRV GAT'ATC
Cuts at: 564 564
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 314 1951 314
Size: 1637 2295
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 615 1366 1614 2636 3615 615
Size: 751 248 1022 979 932
Fragments arranged by size:
1022 979 932 751 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 435 467 538 570 1774 1804 1806 1836 435
Size: 32 71 32 1204 30 2 30 2531
Fragments arranged by size:
2531 1204 71 32 32 30 30 2
HincII GTy'rAC
Cuts at: 971 971
Size: 3932
KasI G'GCGC_C
Cuts at: 1362 1610 1362
Size: 248 3684
KpnI G_GTAC'C
Cuts at: 952 952
Size: 3932
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 2603 2787 326
Size: 2277 184 1471
Fragments arranged by size:
2277 1471 184
MscI TGG'CCA
Cuts at: 802 1693 802
Size: 891 3041
MslI CAynn'nnrTG
Cuts at: 1921 2048 1921
Size: 127 3805
MspA1I CmG'CkG
Cuts at: 153 706 1043 1357 1717 2730 2975 153
Size: 553 337 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 553 360 337 314 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 1363 1611 1363
Size: 248 3684
NcoI C'CATG_G
Cuts at: 427 427
Size: 3932
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NspI r_CATG'y
Cuts at: 478 985 1485 2392 478
Size: 507 500 907 2018
Fragments arranged by size:
2018 907 507 500
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PflMI CCAn_nnn'nTGG
Cuts at: 247 699 247
Size: 452 3480
PfoI T'CCnGG_A
Cuts at: 397 397
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3900
PpuMI rG'GwC_Cy
Cuts at: 188 423 188
Size: 235 3697
PstI C_TGCA'G
Cuts at: 979 1664 979
Size: 685 3247
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 706 1357 1717 706
Size: 651 360 2921
Fragments arranged by size:
2921 651 360
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 656 946 656
Size: 290 3642
SacII CC_GC'GG
Cuts at: 154 154
Size: 3932
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SanDI GG'GwC_CC
Cuts at: 188 188
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 3449 3449
Size: 3932
SfcI C'TryA_G
Cuts at: 33 975 1660 2653 2844 33
Size: 942 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 191
SfoI GGC'GCC
Cuts at: 1364 1612 1364
Size: 248 3684
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 186 954 186
Size: 768 3164
SmlI C'TyrA_G
Cuts at: 1087 2002 2494 2756 1087
Size: 915 492 262 2263
Fragments arranged by size:
2263 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
StuI AGG'CCT
Cuts at: 541 541
Size: 3932
StyI C'CwwG_G
Cuts at: 427 1066 1420 427
Size: 639 354 2939
Fragments arranged by size:
2939 639 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1515 1648 2107 2316 1515
Size: 133 459 209 3131
Fragments arranged by size:
3131 459 209 133
TatI w'GTAC_w
Cuts at: 3447 3447
Size: 3932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 393 425 3449 3481 393
Size: 32 3024 32 844
Fragments arranged by size:
3024 844 32 32
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1427 1427
Size: 3932
XmaI C'CCGG_G
Cuts at: 184 952 184
Size: 768 3164
XmnI GAAnn'nnTTC
Cuts at: 297 297
Size: 3932
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI