Restriction map for
pF1KB0999
(Circular) (Six-base) MAPSORT of: pF1KB0999.seq Check: 6059 from: 1 to: 10463
pF1KB0999 10463 bp
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 365 2875 4979 5357 5883 6183 6455 365
Size: 2510 2104 378 526 300 272 4373
Fragments arranged by size:
4373 2510 2104 526 378 300 272
AatII G_ACGT'C
Cuts at: 218 3842 4891 6769 218
Size: 3624 1049 1878 3912
Fragments arranged by size:
3912 3624 1878 1049
AccI GT'mk_AC
Cuts at: 4891 5915 7501 4891
Size: 1024 1586 7853
Fragments arranged by size:
7853 1586 1024
Acc65I G'GTAC_C
Cuts at: 7479 7479
Size: 10463
AclI AA'CG_TT
Cuts at: 10303 10303
Size: 10463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 706 842 2140 2291 2528 3016 3025 3606
Size: 182 136 1298 151 237 488 9 581
Cuts at: 3606 4366 4372 4675 5418 6930 8288 8720 9073
Size: 760 6 303 743 1512 1358 432 353
Cuts at: 9073 9735 524
Size: 662 1252
Fragments arranged by size:
1512 1358 1298 1252 760 743 662 581
488 432 353 303 237 182 151 136
9 6
AfeI AGC'GCT
Cuts at: 1009 1009
Size: 10463
AflIII A'CryG_T
Cuts at: 1397 7815 9615 9896 1397
Size: 6418 1800 281 1964
Fragments arranged by size:
6418 1964 1800 281
AgeI A'CCGG_T
Cuts at: 546 546
Size: 10463
AleI CACnn'nnGTG
Cuts at: 138 3778 5590 5797 6548 138
Size: 3640 1812 207 751 4053
Fragments arranged by size:
4053 3640 1812 751 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 802 3515 3796 802
Size: 2713 281 7469
Fragments arranged by size:
7469 2713 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 982 1014 6227 6259 982
Size: 32 5213 32 5186
Fragments arranged by size:
5213 5186 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1498 2329 3255 4335 5071 6237 7768 9206 1498
Size: 831 926 1080 736 1166 1531 1438 2755
Fragments arranged by size:
2755 1531 1438 1166 1080 926 831 736
ApaI G_GGCC'C
Cuts at: 857 2408 3571 4342 7376 857
Size: 1551 1163 771 3034 3944
Fragments arranged by size:
3944 3034 1551 1163 771
ApaLI G'TGCA_C
Cuts at: 2703 4591 7204 9301 2703
Size: 1888 2613 2097 3865
Fragments arranged by size:
3865 2613 2097 1888
ApoI r'AATT_y
Cuts at: 7467 9816 10072 7467
Size: 2349 256 7858
Fragments arranged by size:
7858 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10463
AvaI C'yCGr_G
Cuts at: 328 385 730 2832 2960 3062 3164 3484 3885
Size: 57 345 2102 128 102 102 320 401
Cuts at: 3885 4441 4619 4683 4933 5315 5960 5984 7161
Size: 556 178 64 250 382 645 24 1177
Cuts at: 7161 7483 328
Size: 322 3308
Fragments arranged by size:
3308 2102 1177 645 556 401 382 345
322 320 250 178 128 102 102 64
57 24
BamHI G'GATC_C
Cuts at: 115 4205 7488 115
Size: 4090 3283 3090
Fragments arranged by size:
4090 3283 3090
BanI G'GyrC_C
Cuts at: 594 1571 2298 2428 2836 3186 3499 3615 3687
Size: 977 727 130 408 350 313 116 72
Cuts at: 3687 3780 3853 4492 4705 4773 5377 5899 6168
Size: 93 73 639 213 68 604 522 269
Cuts at: 6168 6578 6703 7479 7893 8141 8176 594
Size: 410 125 776 414 248 35 2881
Fragments arranged by size:
2881 977 776 727 639 604 522 414
410 408 350 313 269 248 213 130
125 116 93 73 72 68 35
BanII G_rGCy'C
Cuts at: 158 224 353 857 2035 2408 2495 2523 2690
Size: 66 129 504 1178 373 87 28 167
Cuts at: 2690 2833 3059 3184 3571 3736 4342 4480 4693
Size: 143 226 125 387 165 606 138 213
Cuts at: 4693 4750 4826 5807 5936 6044 6067 6074 6296
Size: 57 76 981 129 108 23 7 222
Cuts at: 6296 6313 6341 6362 6893 6907 7376 7412 7477
Size: 17 28 21 531 14 469 36 65
Cuts at: 7477 8507 158
Size: 1030 2114
Fragments arranged by size:
2114 1178 1030 981 606 531 504 469
387 373 226 222 213 167 165 143
138 129 129 125 108 87 76 66
65 57 36 28 28 23 21 17
14 7
BbeI G_GCGC'C
Cuts at: 1575 2432 2840 4709 6172 7897 8145 1575
Size: 857 408 1869 1463 1725 248 3893
Fragments arranged by size:
3893 1869 1725 1463 857 408 248
BbsI GAAGACnn'nnnn_
Cuts at: 512 1495 1742 1759 1842 512
Size: 983 247 17 83 9133
Fragments arranged by size:
9133 983 247 83 17
BbvCI CC'TCA_GC
Cuts at: 2651 2756 3143 3348 4101 5260 2651
Size: 105 387 205 753 1159 7854
Fragments arranged by size:
7854 1159 753 387 205 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2170 2204 2528 2562 2842 2876 4957 4991 2170
Size: 34 324 34 280 34 2081 34 7642
Fragments arranged by size:
7642 2081 324 280 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8361 9417 10463 8361
Size: 1056 1046 8361
Fragments arranged by size:
8361 1056 1046
BclI T'GATC_A
Cuts at: 3317 7307 7983 3317
Size: 3990 676 5797
Fragments arranged by size:
5797 3990 676
BglI GCCn_nnn'nGGC
Cuts at: 294 533 603 1178 1918 2758 2773 3167 3703
Size: 239 70 575 740 840 15 394 536
Cuts at: 3703 4085 5894 5979 294
Size: 382 1809 85 4778
Fragments arranged by size:
4778 1809 840 740 575 536 394 382
239 85 70 15
BglII A'GATC_T
Cuts at: 674 2099 7067 7978 10015 674
Size: 1425 4968 911 2037 1122
Fragments arranged by size:
4968 2037 1425 1122 911
BlpI GC'TnA_GC
Cuts at: 6595 7575 6595
Size: 980 9483
Bme1580I G_kGCm'C
Cuts at: 857 2303 2408 2707 3305 3571 3692 3785 3856
Size: 1446 105 299 598 266 121 93 71
Cuts at: 3856 4279 4342 4495 4595 4626 4776 4934 5366
Size: 423 63 153 100 31 150 158 432
Cuts at: 5366 6708 6839 7208 7376 8088 8181 9305 857
Size: 1342 131 369 168 712 93 1124 2015
Fragments arranged by size:
2015 1446 1342 1124 712 598 432 423
369 299 266 168 158 153 150 131
121 105 100 93 93 71 63 31
BmgBI CAC'GTC
Cuts at: 140 3534 3933 3960 5504 5579 140
Size: 3394 399 27 1544 75 5024
Fragments arranged by size:
5024 3394 1544 399 75 27
BmrI ACTGGGnnnn_n'
Cuts at: 181 2608 3987 5750 5902 6311 6665 7675 7756
Size: 2427 1379 1763 152 409 354 1010 81
Cuts at: 7756 8091 10235 181
Size: 335 2144 409
Fragments arranged by size:
2427 2144 1763 1379 1010 409 409 354
335 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 390 1938 6063 6984 7461 8830 390
Size: 1548 4125 921 477 1369 2023
Fragments arranged by size:
4125 2023 1548 1369 921 477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 607 727 1282 1501 2841 5188 5631 5728
Size: 360 120 555 219 1340 2347 443 97
Cuts at: 5728 6058 6397 6475 6734 6899 247
Size: 330 339 78 259 165 3811
Fragments arranged by size:
3811 2347 1340 555 443 360 339 330
259 219 165 120 97 78
Bpu10I CC'TnA_GC
Cuts at: 581 2651 2756 3143 3348 4023 4101 4724 5260
Size: 2070 105 387 205 675 78 623 536
Cuts at: 5260 581
Size: 5784
Fragments arranged by size:
5784 2070 675 623 536 387 205 105
78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2764 4453 4486 7639 8518 9232 9530 1729
Size: 1035 1689 33 3153 879 714 298 2662
Fragments arranged by size:
3153 2662 1689 1035 879 714 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 1414 1990 2590 4123 5519 5607 5745 6752 7125
Size: 576 600 1533 1396 88 138 1007 373
Cuts at: 7125 10167 1414
Size: 3042 1710
Fragments arranged by size:
3042 1710 1533 1396 1007 600 576 373
138 88
BsaAI yAC'GTr
Cuts at: 4221 7816 4221
Size: 3595 6868
BsaBI GATnn'nnATC
Cuts at: 6 7977 10014 6
Size: 7971 2037 455
Fragments arranged by size:
7971 2037 455
BsaHI Gr'CG_yC
Cuts at: 215 1572 2227 2429 2837 3137 3706 3839 4616
Size: 1357 655 202 408 300 569 133 777
Cuts at: 4616 4706 4888 4894 6169 6766 7234 7894 8142
Size: 90 182 6 1275 597 468 660 248
Cuts at: 8142 8844 10340 215
Size: 702 1496 338
Fragments arranged by size:
1496 1357 1275 777 702 660 655 597
569 468 408 338 300 248 202 182
133 90 6
BsaWI w'CCGG_w
Cuts at: 546 809 2466 2515 3672 6813 7025 7090 7652
Size: 263 1657 49 1157 3141 212 65 562
Cuts at: 7652 8173 8851 9262 9409 9683 546
Size: 521 678 411 147 274 1326
Fragments arranged by size:
3141 1657 1326 1157 678 562 521 411
274 263 212 147 65 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 871 901 982 1012 2839 2869 3631
Size: 30 600 30 81 30 1827 30 762
Cuts at: 3631 3661 3796 3826 4537 4567 5020 5050 5437
Size: 30 135 30 711 30 453 30 387
Cuts at: 5437 5467 5604 5634 6227 6257 7340 7370 241
Size: 30 137 30 593 30 1083 30 3334
Fragments arranged by size:
3334 1827 1083 762 711 600 593 453
387 137 135 81 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 631 724 727 978 1675 1717 1720 1732 1900
Size: 93 3 251 697 42 3 12 168
Cuts at: 1900 2197 2230 2263 2266 2278 2357 2552 2596
Size: 297 33 33 3 12 79 195 44
Cuts at: 2596 2605 2608 2635 2644 2668 2698 2734 2929
Size: 9 3 27 9 24 30 36 195
Cuts at: 2929 3450 4089 4438 4660 5832 6039 7188 7420
Size: 521 639 349 222 1172 207 1149 232
Cuts at: 7420 631
Size: 3674
Fragments arranged by size:
3674 1172 1149 697 639 521 349 297
251 232 222 207 195 195 168 93
79 44 42 36 33 33 30 27
24 12 12 9 9 3 3 3
3
BseYI C'CCAG_C
Cuts at: 290 390 559 1901 2322 2415 2932 3096 3228
Size: 100 169 1342 421 93 517 164 132
Cuts at: 3228 3475 3664 3769 4728 5243 5382 5426 5806
Size: 247 189 105 959 515 139 44 380
Cuts at: 5806 6021 6055 6539 6892 7132 7888 8646 9311
Size: 215 34 484 353 240 756 758 665
Cuts at: 9311 9719 290
Size: 408 1034
Fragments arranged by size:
1342 1034 959 758 756 665 517 515
484 421 408 380 353 247 240 215
189 169 164 139 132 105 100 93
44 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 808 812 2540 2894 211
Size: 597 4 1728 354 7780
Fragments arranged by size:
7780 1728 597 354 4
BsiEI CG_ry'CG
Cuts at: 104 466 1202 2001 2768 3906 4046 4465 4575
Size: 362 736 799 767 1138 140 419 110
Cuts at: 4575 6454 8005 8051 9281 104
Size: 1879 1551 46 1230 1286
Fragments arranged by size:
1879 1551 1286 1230 1138 799 767 736
419 362 140 110 46
BsiHKAI G_wGCw'C
Cuts at: 353 1217 2707 2833 4000 4595 4940 4970 5360
Size: 864 1490 126 1167 595 345 30 390
Cuts at: 5360 5600 6313 6362 7208 7477 8255 8445 9305
Size: 240 713 49 846 269 778 190 860
Cuts at: 9305 353
Size: 1511
Fragments arranged by size:
1511 1490 1167 864 860 846 778 713
595 390 345 269 240 190 126 49
30
BsiWI C'GTAC_G
Cuts at: 4548 4548
Size: 10463
BsmI GAATG_Cn'
Cuts at: 2191 2317 3764 2191
Size: 126 1447 8890
Fragments arranged by size:
8890 1447 126
BsmBI CGTCTCn'nnnn_
Cuts at: 1551 4033 1551
Size: 2482 7981
Bsp1286I G_dGCh'C
Cuts at: 158 224 353 857 1217 2035 2303 2408 2495
Size: 66 129 504 360 818 268 105 87
Cuts at: 2495 2523 2690 2707 2833 3059 3184 3305 3571
Size: 28 167 17 126 226 125 121 266
Cuts at: 3571 3692 3736 3785 3856 4000 4279 4342 4480
Size: 121 44 49 71 144 279 63 138
Cuts at: 4480 4495 4595 4626 4693 4750 4776 4826 4934
Size: 15 100 31 67 57 26 50 108
Cuts at: 4934 4940 4970 5360 5366 5600 5807 5936 6044
Size: 6 30 390 6 234 207 129 108
Cuts at: 6044 6067 6074 6296 6313 6341 6362 6708 6839
Size: 23 7 222 17 28 21 346 131
Cuts at: 6839 6893 6907 7208 7376 7412 7477 8088 8181
Size: 54 14 301 168 36 65 611 93
Cuts at: 8181 8255 8445 8507 9305 158
Size: 74 190 62 798 1316
Fragments arranged by size:
1316 818 798 611 504 390 360 346
301 279 268 266 234 226 222 207
190 168 167 144 138 131 129 129
126 125 121 121 108 108 105 100
93 87 74 71 67 66 65 63
62 57 54 50 49 44 36 31
30 28 28 26 23 21 17 17
15 14 7 6 6
BspHI T'CATG_A
Cuts at: 906 1391 8895 906
Size: 485 7504 2474
Fragments arranged by size:
7504 2474 485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 365 389 2875 3128 4250 4979 5090 5357 5883
Size: 24 2486 253 1122 729 111 267 526
Cuts at: 5883 6183 6455 7267 7513 7727 8029 8410 365
Size: 300 272 812 246 214 302 381 2418
Fragments arranged by size:
2486 2418 1122 812 729 526 381 302
300 272 267 253 246 214 111 24
BsrBI CCG'CTC
Cuts at: 50 1859 2240 2807 3016 4400 4467 4500 5517
Size: 1809 381 567 209 1384 67 33 1017
Cuts at: 5517 5541 5559 5979 6029 7239 8755 8809 10293
Size: 24 18 420 50 1210 1516 54 1484
Cuts at: 10293 10458 50
Size: 165 55
Fragments arranged by size:
1809 1516 1484 1384 1210 1017 567 420
381 209 165 67 55 54 50 33
24 18
BsrDI GCAATG_nn'
Cuts at: 4694 8375 4694
Size: 3681 6782
BsrFI r'CCGG_y
Cuts at: 546 2166 2727 2912 4356 4513 4661 4783 5174
Size: 1620 561 185 1444 157 148 122 391
Cuts at: 5174 5995 6165 7181 8461 8642 546
Size: 821 170 1016 1280 181 2367
Fragments arranged by size:
2367 1620 1444 1280 1016 821 561 391
185 181 170 157 148 122
BsrGI T'GTAC_A
Cuts at: 875 1373 3845 875
Size: 498 2472 7493
Fragments arranged by size:
7493 2472 498
BssHII G'CGCG_C
Cuts at: 4699 4699
Size: 10463
BssSI C'ACGA_G
Cuts at: 1216 4796 4898 5359 8734 9442 1216
Size: 3580 102 461 3375 708 2237
Fragments arranged by size:
3580 3375 2237 708 461 102
BstAPI GCAn_nnn'nTGC
Cuts at: 1641 3120 4780 5071 5214 7840 1641
Size: 1479 1660 291 143 2626 4264
Fragments arranged by size:
4264 2626 1660 1479 291 143
BstBI TT'CG_AA
Cuts at: 8824 8824
Size: 10463
BstEII G'GTnAC_C
Cuts at: 3039 3373 6256 6514 6528 3039
Size: 334 2883 258 14 6974
Fragments arranged by size:
6974 2883 334 258 14
BstXI CCAn_nnnn'nTGG
Cuts at: 3612 5238 5394 5592 6351 6900 3612
Size: 1626 156 198 759 549 7175
Fragments arranged by size:
7175 1626 759 549 198 156
BstYI r'GATC_y
Cuts at: 1 115 499 674 1124 2099 4205 4412 7067
Size: 114 384 175 450 975 2106 207 2655
Cuts at: 7067 7488 7955 7978 8313 8559 8865 8877 8963
Size: 421 467 23 335 246 306 12 86
Cuts at: 8963 10015 1
Size: 1052 449
Fragments arranged by size:
2655 2106 1052 975 467 450 449 421
384 335 306 246 207 175 114 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1767 5824 1767
Size: 4057 6406
BtgI C'CryG_G
Cuts at: 482 601 659 1575 1925 2201 2645 2762 3578
Size: 119 58 916 350 276 444 117 816
Cuts at: 3578 3596 3652 3807 3822 3900 4185 4379 4459
Size: 18 56 155 15 78 285 194 80
Cuts at: 4459 4738 4767 5046 5142 5237 5393 5816 6261
Size: 279 29 279 96 95 156 423 445
Cuts at: 6261 6699 6714 6974 7361 482
Size: 438 15 260 387 3584
Fragments arranged by size:
3584 916 816 445 444 438 423 387
350 285 279 279 276 260 194 156
155 119 117 96 95 80 78 58
56 29 18 15 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 946 2001 2531 6096 8413 8594 8757 946
Size: 1055 530 3565 2317 181 163 2652
Fragments arranged by size:
3565 2652 2317 1055 530 181 163
BtsI GCAGTG_nn'
Cuts at: 1188 2056 2322 2669 2877 3379 3401 5883 7417
Size: 868 266 347 208 502 22 2482 1534
Cuts at: 7417 7706 1188
Size: 289 3945
Fragments arranged by size:
3945 2482 1534 868 502 347 289 266
208 22
ClaI AT'CG_AT
Cuts at: 1235 1235
Size: 10463
DraI TTT'AAA
Cuts at: 7462 7462
Size: 10463
DraIII CAC_nnn'GTG
Cuts at: 1221 6673 1221
Size: 5452 5011
DrdI GACnn_nn'nnGTC
Cuts at: 1387 8169 9513 1387
Size: 6782 1344 2337
Fragments arranged by size:
6782 2337 1344
EaeI y'GGCC_r
Cuts at: 361 479 1199 1283 1355 1998 2012 2174 2729
Size: 118 720 84 72 643 14 162 555
Cuts at: 2729 2765 3903 4043 4376 4462 4572 4610 4663
Size: 36 1138 140 333 86 110 38 53
Cuts at: 4663 4785 5228 5971 5997 6099 6451 7183 8048
Size: 122 443 743 26 102 352 732 865
Cuts at: 8048 8222 8613 8640 9985 361
Size: 174 391 27 1345 839
Fragments arranged by size:
1345 1138 865 839 743 732 720 643
555 443 391 352 333 174 162 140
122 118 110 102 86 84 72 53
38 36 27 26 14
EagI C'GGCC_G
Cuts at: 1199 1998 2765 3903 4043 4462 4572 6451 8048
Size: 799 767 1138 140 419 110 1879 1597
Cuts at: 8048 1199
Size: 3614
Fragments arranged by size:
3614 1879 1597 1138 799 767 419 140
110
EarI CTCTTCn'nnn_
Cuts at: 2074 4651 6350 8486 8696 2074
Size: 2577 1699 2136 210 3841
Fragments arranged by size:
3841 2577 2136 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 312 1708 3761 4274 6649 6960 8671 9413 9559
Size: 1396 2053 513 2375 311 1711 742 146
Cuts at: 9559 10273 312
Size: 714 502
Fragments arranged by size:
2375 2053 1711 1396 742 714 513 502
311 146
EcoICRI GAG'CTC
Cuts at: 351 2831 6311 6360 7475 351
Size: 2480 3480 49 1115 3339
Fragments arranged by size:
3480 3339 2480 1115 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 524 607 706 727 842 1282 1501 2140
Size: 277 83 99 21 115 440 219 639
Cuts at: 2140 2291 2528 2841 3016 3025 3606 4366 4372
Size: 151 237 313 175 9 581 760 6
Cuts at: 4372 4675 5188 5418 5631 5728 6058 6397 6475
Size: 303 513 230 213 97 330 339 78
Cuts at: 6475 6734 6899 6930 8288 8720 9073 9735 247
Size: 259 165 31 1358 432 353 662 975
Fragments arranged by size:
1358 975 760 662 639 581 513 440
432 353 339 330 313 303 277 259
237 230 219 213 175 165 151 115
99 97 83 78 31 21 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 514 1744 3847 3922 4312 4840 514
Size: 1230 2103 75 390 528 6137
Fragments arranged by size:
6137 2103 1230 528 390 75
EcoO109I rG'GnC_Cy
Cuts at: 302 853 854 3264 3558 3568 4027 4305 4332
Size: 551 1 2410 294 10 459 278 27
Cuts at: 4332 4339 4368 5265 5311 5955 6406 7265 7373
Size: 7 29 897 46 644 451 859 108
Cuts at: 7373 7602 302
Size: 229 3163
Fragments arranged by size:
3163 2410 897 859 644 551 459 451
294 278 229 108 46 29 27 10
7 1
EcoRI G'AATT_C
Cuts at: 7467 7467
Size: 10463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 966 1440 8482 966
Size: 474 7042 2947
Fragments arranged by size:
7042 2947 474
FseI GG_CCGG'CC
Cuts at: 2731 2731
Size: 10463
FspI TGC'GCA
Cuts at: 8244 8244
Size: 10463
HaeII r_GCGC'y
Cuts at: 429 1011 1575 1913 2167 2432 2611 2779 2840
Size: 582 564 338 254 265 179 168 61
Cuts at: 2840 3797 4709 5167 6172 7897 8145 9375 10146
Size: 957 912 458 1005 1725 248 1230 771
Cuts at: 10146 429
Size: 746
Fragments arranged by size:
1725 1230 1005 957 912 771 746 582
564 458 338 265 254 248 179 168
61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 954 986 1228 1260 1750 1782 2839
Size: 32 681 32 242 32 490 32 1057
Cuts at: 2839 2871 3631 3663 4537 4569 5020 5052 5309
Size: 32 760 32 874 32 451 32 257
Cuts at: 5309 5341 7252 7284 8305 8335 8337 8367 241
Size: 32 1911 32 1021 30 2 30 2337
Fragments arranged by size:
2337 1911 1057 1021 874 760 681 490
451 257 242 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2334 4892 7502 2334
Size: 2558 2610 5295
Fragments arranged by size:
5295 2610 2558
HindIII A'AGCT_T
Cuts at: 6415 6415
Size: 10463
KasI G'GCGC_C
Cuts at: 1571 2428 2836 4705 6168 7893 8141 1571
Size: 857 408 1869 1463 1725 248 3893
Fragments arranged by size:
3893 1869 1725 1463 857 408 248
KpnI G_GTAC'C
Cuts at: 7483 7483
Size: 10463
MluI A'CGCG_T
Cuts at: 9896 9896
Size: 10463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 914 3464 5500 7472 9222 9406 914
Size: 2550 2036 1972 1750 184 1971
Fragments arranged by size:
2550 2036 1972 1971 1750 184
MscI TGG'CCA
Cuts at: 481 4378 6101 8224 481
Size: 3897 1723 2123 2720
Fragments arranged by size:
3897 2720 2123 1723
MslI CAynn'nnrTG
Cuts at: 138 1339 1369 1396 3778 3985 5236 5392 5590
Size: 1201 30 27 2382 207 1251 156 198
Cuts at: 5590 5696 5776 5797 6548 8452 8579 138
Size: 106 80 21 751 1904 127 2022
Fragments arranged by size:
2382 2022 1904 1251 1201 751 207 198
156 127 106 80 30 27 21
MspA1I CmG'CkG
Cuts at: 294 705 873 1176 1281 1577 2647 2755 3003
Size: 411 168 303 105 296 1070 108 248
Cuts at: 3003 3626 3645 3809 3835 3902 4187 4461 4599
Size: 623 19 164 26 67 285 274 138
Cuts at: 4599 4740 5048 5071 5144 5636 5645 5654 5797
Size: 141 308 23 73 492 9 9 143
Cuts at: 5797 6456 6486 6522 6641 6861 7363 7370 7574
Size: 659 30 36 119 220 502 7 204
Cuts at: 7574 7888 8248 9032 9277 294
Size: 314 360 784 245 1480
Fragments arranged by size:
1480 1070 784 659 623 502 492 411
360 314 308 303 296 285 274 248
245 220 204 168 164 143 141 138
119 108 105 73 67 36 30 26
23 19 9 9 7
NaeI GCC'GGC
Cuts at: 2168 2729 4358 4663 4785 6167 8644 2168
Size: 561 1629 305 122 1382 2477 3987
Fragments arranged by size:
3987 2477 1629 1382 561 305 122
NarI GG'CG_CC
Cuts at: 1572 2429 2837 4706 6169 7894 8142 1572
Size: 857 408 1869 1463 1725 248 3893
Fragments arranged by size:
3893 1869 1725 1463 857 408 248
NcoI C'CATG_G
Cuts at: 659 1925 3578 3596 5237 5393 6699 6974 659
Size: 1266 1653 18 1641 156 1306 275 4148
Fragments arranged by size:
4148 1653 1641 1306 1266 275 156 18
NgoMIV G'CCGG_C
Cuts at: 2166 2727 4356 4661 4783 6165 8642 2166
Size: 561 1629 305 122 1382 2477 3987
Fragments arranged by size:
3987 2477 1629 1382 561 305 122
NotI GC'GGCC_GC
Cuts at: 4462 6451 4462
Size: 1989 8474
NspI r_CATG'y
Cuts at: 1158 1401 2284 3337 3553 3984 7516 8016 9619
Size: 243 883 1053 216 431 3532 500 1603
Cuts at: 9619 1158
Size: 2002
Fragments arranged by size:
3532 2002 1603 1053 883 500 431 243
216
PasI CC'CwG_GG
Cuts at: 2424 3387 4335 6502 6631 2424
Size: 963 948 2167 129 6256
Fragments arranged by size:
6256 2167 963 948 129
PciI A'CATG_T
Cuts at: 1397 9615 1397
Size: 8218 2245
PflMI CCAn_nnn'nTGG
Cuts at: 633 1023 6237 6525 633
Size: 390 5214 288 4571
Fragments arranged by size:
5214 4571 390 288
PfoI T'CCnGG_A
Cuts at: 224 1427 1478 1988 6230 6650 6841 224
Size: 1203 51 510 4242 420 191 3846
Fragments arranged by size:
4242 3846 1203 510 420 191 51
PmeI GTTT'AAAC
Cuts at: 7462 7462
Size: 10463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 394 426 982 1014 1099 1131 6227 6259 8909
Size: 32 556 32 85 32 5096 32 2650
Cuts at: 8909 8941 394
Size: 32 1916
Fragments arranged by size:
5096 2650 1916 556 85 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3264 4027 4305 5311 3264
Size: 763 278 1006 8416
Fragments arranged by size:
8416 1006 763 278
PshAI GACnn'nnGTC
Cuts at: 6769 6769
Size: 10463
PspOMI G'GGCC_C
Cuts at: 853 2404 3567 4338 7372 853
Size: 1551 1163 771 3034 3944
Fragments arranged by size:
3944 3034 1551 1163 771
PstI C_TGCA'G
Cuts at: 1148 2872 3125 4841 7322 7510 8195 1148
Size: 1724 253 1716 2481 188 685 3416
Fragments arranged by size:
3416 2481 1724 1716 685 253 188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10463
PvuII CAG'CTG
Cuts at: 705 1281 3003 3645 3835 6641 7888 8248 705
Size: 576 1722 642 190 2806 1247 360 2920
Fragments arranged by size:
2920 2806 1722 1247 642 576 360 190
RsrII CG'GwC_CG
Cuts at: 8658 8658
Size: 10463
SacI G_AGCT'C
Cuts at: 353 2833 6313 6362 7477 353
Size: 2480 3480 49 1115 3339
Fragments arranged by size:
3480 3339 2480 1115 49
SacII CC_GC'GG
Cuts at: 1578 2648 3810 3903 4188 4462 4741 5049 5145
Size: 1070 1162 93 285 274 279 308 96
Cuts at: 5145 7364 1578
Size: 2219 4677
Fragments arranged by size:
4677 2219 1162 1070 308 285 279 274
96 93
SalI G'TCGA_C
Cuts at: 4890 7500 4890
Size: 2610 7853
SanDI GG'GwC_CC
Cuts at: 3264 5311 3264
Size: 2047 8416
SapI GCTCTTCn'nnn_
Cuts at: 4651 6350 8486 8696 4651
Size: 1699 2136 210 6418
Fragments arranged by size:
6418 2136 1699 210
SbfI CC_TGCA'GG
Cuts at: 4841 7510 4841
Size: 2669 7794
ScaI AGT'ACT
Cuts at: 1102 9980 1102
Size: 8878 1585
SexAI A'CCwGG_T
Cuts at: 3369 5616 3369
Size: 2247 8216
SfcI C'TryA_G
Cuts at: 33 1144 2868 3121 4837 6273 7318 7506 8191
Size: 1111 1724 253 1716 1436 1045 188 685
Cuts at: 8191 9159 9350 33
Size: 968 191 1146
Fragments arranged by size:
1724 1716 1436 1146 1111 1045 968 685
253 191 188
SfiI GGCCn_nnn'nGGCC
Cuts at: 2758 5979 2758
Size: 3221 7242
SfoI GGC'GCC
Cuts at: 1573 2430 2838 4707 6170 7895 8143 1573
Size: 857 408 1869 1463 1725 248 3893
Fragments arranged by size:
3893 1869 1725 1463 857 408 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10463
SgrAI Cr'CCGG_yG
Cuts at: 2166 4513 6165 2166
Size: 2347 1652 6464
Fragments arranged by size:
6464 2347 1652
SmaI CCC'GGG
Cuts at: 330 2962 3064 3166 4621 5962 7163 7485 330
Size: 2632 102 102 1455 1341 1201 322 3308
Fragments arranged by size:
3308 2632 1455 1341 1201 322 102 102
SmlI C'TyrA_G
Cuts at: 1744 2779 3484 4468 4501 7618 8533 9247 9509
Size: 1035 705 984 33 3117 915 714 262
Cuts at: 9509 1744
Size: 2698
Fragments arranged by size:
3117 2698 1035 984 915 714 705 262
33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10463
SphI G_CATG'C
Cuts at: 1158 3337 3553 7516 8016 1158
Size: 2179 216 3963 500 3605
Fragments arranged by size:
3963 3605 2179 500 216
SrfI GCCC'GGGC
Cuts at: 4621 4621
Size: 10463
StuI AGG'CCT
Cuts at: 848 1030 3325 4318 848
Size: 182 2295 993 6993
Fragments arranged by size:
6993 2295 993 182
StyI C'CwwG_G
Cuts at: 515 611 659 1079 1925 2384 2576 2732 2926
Size: 96 48 420 846 459 192 156 194
Cuts at: 2926 3510 3554 3578 3596 3923 4001 5237 5393
Size: 584 44 24 18 327 78 1236 156
Cuts at: 5393 5948 6699 6974 7115 7597 7951 515
Size: 555 751 275 141 482 354 3027
Fragments arranged by size:
3027 1236 846 751 584 555 482 459
420 354 327 275 194 192 156 156
141 96 78 48 44 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 662 2469 3392 3444 3510 3835 4202 4327 4892
Size: 1807 923 52 66 325 367 125 565
Cuts at: 4892 4958 5619 5896 6330 6599 6684 6759 6766
Size: 66 661 277 434 269 85 75 7
Cuts at: 6766 6973 7033 8046 8179 8638 8847 662
Size: 207 60 1013 133 459 209 2278
Fragments arranged by size:
2278 1807 1013 923 661 565 459 434
367 325 277 269 209 207 133 125
85 75 66 66 60 52 7
TatI w'GTAC_w
Cuts at: 875 953 1100 1373 3845 9978 875
Size: 78 147 273 2472 6133 1360
Fragments arranged by size:
6133 2472 1360 273 147 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 3949 3981 4190 4222 5437 5469 7340
Size: 32 3754 32 209 32 1215 32 1871
Cuts at: 7340 7372 9980 10012 163
Size: 32 2608 32 614
Fragments arranged by size:
3754 2608 1871 1215 614 209 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2043 8260 2043
Size: 6217 4246
XbaI T'CTAG_A
Cuts at: 7494 7494
Size: 10463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 126 1180 2022 3256 3561 5589 6234 7958 126
Size: 1054 842 1234 305 2028 645 1724 2631
Fragments arranged by size:
2631 2028 1724 1234 1054 842 645 305
XhoI C'TCGA_G
Cuts at: 3484 3484
Size: 10463
XmaI C'CCGG_G
Cuts at: 328 2960 3062 3164 4619 5960 7161 7483 328
Size: 2632 102 102 1455 1341 1201 322 3308
Fragments arranged by size:
3308 2632 1455 1341 1201 322 102 102
XmnI GAAnn'nnTTC
Cuts at: 1114 1114
Size: 10463
ZraI GAC'GTC
Cuts at: 216 3840 4889 6767 216
Size: 3624 1049 1878 3912
Fragments arranged by size:
3912 3624 1878 1049
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BfrBI BmtI BspEI
BstZ17I CspCI EcoRV FspAI HpaI MfeI NdeI NheI
NruI NsiI PacI PmlI PsiI PspXI PsrI SnaBI
SspI SwaI